Homogenizer PowerLyzer 24 User manual

Sample to Insight__
May 2022
PowerLyzer®24
Homogenizer
User Manual
For lysis and homogenization of biological samples
contained in bead beating tubes at variable speeds

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PowerLyzer 24 Homogenizer User Manual 05/2022
Introduction
Before using the PowerLyzer 24 Homogenizer, it is essential you read this user manual
carefully and pay particular attention to the safety information. The instructions and safety
information in this user manual must be followed to ensure safe operation of the PowerLyzer
24 Homogenizer and to maintain the PowerLyzer 24 Homogenizer in a safe condition.
Intended use of the PowerLyzer 24 Homogenizer
The PowerLyzer 24 Homogenizer is intended for molecular biology applications. This product
is neither intended for the diagnosis, prevention, or treatment of a disease, nor has it been
validated for such use either alone or in combination with other products. Therefore, the
performance characteristics of the product for clinical use (i.e., diagnostic, prognostic,
therapeutic or blood banking) are unknown.
Requirements for PowerLyzer 24 Homogenizer users
This table covers the general level of competence and training necessary for transportation,
installation, use, maintenance and servicing of the PowerLyzer 24 Homogenizer.
Task Personnel Training and experience
Transportation No special requirements No special requirements
Installation, routine use and
maintenance
Laboratory technicians or equivalent Appropriately trained and
experienced personnel
Servicing QIAGEN Instrument Service
Specialists only
–

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General Description
The PowerLyzer 24 Homogenizer has been designed to lyse and homogenize biological
samples contained in bead beating tubes at variable speeds. It can simultaneously process
up to 24 samples in 2 ml tubes at very high speed.
Features of the PowerLyzer 24 Homogenizer system
Simultaneous homogenization of 24 x 2 ml tubes
Flexibility for any type of sample processing; broad speed range, number of cycles, time,
dwell/pause
Unique front-loading tube position for ease of use and optimal bead beating interaction
Processing of hundreds of samples per day
Up to 99 custom programmable settings
Disposable tubes to ensure no threat of cross-contamination or sample degradation
Installation Procedures
Parts
Prior to operation, remove all parts from the shipping container and inspect for damaged or
missing parts. If any parts are found to be damaged or missing, please contact QIAGEN
Technical Support.

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PowerLyzer 24 Homogenizer User Manual 05/2022
The PowerLyzer 24 Homogenizer consists of the following parts.
Description Quantity
Motor Drive Unit (115V or 230V) 1
Finger Plate (black plastic) 1
Tube Holder (blue anodized aluminum) 1
Power Cords (US, EU, UK, AUS) 1 Each
Spare Fuse 1
User Manual 1
Unpacking the PowerLyzer 24 Homogenizer
WARNING
Risk of personal injury and material damage [W6]
Do not connect the unit to the main power supply until installation is
complete and the correct voltage is selected. Do not turn the unit upside
down. The PowerLyzer 24 Homogenizer must always rest on its four feet to
avoid damaging internal components or breaking the housing.

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Note: Do not discard the crate and packaging foam. This packaging must be used in the
event the PowerLyzer 24 Homogenizer needs to be returned to QIAGEN for any reason.
Shipping the PowerLyzer 24 Homogenizer in any other packaging will void all warranties.
1. Using a screwdriver or power drill, remove the 16 screws around the bottom edge of the
crate.
2. Lift the lid from the crate.
3. Unscrew the two external knob screws from the back of the unit to release from the
support plate.

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PowerLyzer 24 Homogenizer User Manual 05/2022
4. Slide the PowerLyzer 24 Homogenizer away from the knob screw holes.
5. Lift the unit up and off of the crate bottom. Do not lift the unit by holding the lid.
Note: This is a heavy item: use caution when lifting to avoid back injury.

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CAUTION
Damage to the instrument [C1]
Do not lift the instrument by holding the door. It must be lifted by holding
the bottom of the unit. Unpack the PowerLyzer 24 Homogenizer with care
and inspect it carefully.
6. Remove the warning label and make sure the voltage is set correctly for your location
before connecting the power cord (see “Installation”, below).
Installation
WARNING
Risk of personal injury and material damage [W6]
Do not connect the unit to the main power supply until installation is
complete and the correct voltage is selected. Do not turn the unit upside
down. The PowerLyzer 24 Homogenizer must always rest on its four feet to
avoid damaging internal components or breaking the housing.

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PowerLyzer 24 Homogenizer User Manual 05/2022
1. Check that the voltage selection switch located at the back of the unit matches the
voltage delivered by your electric power supply (15 = 115 V, 30 = 230 V). Use a small
screwdriver to slide the red switch up or down to select the correct power supply. Select
“15” for 110–120 V or “30” for 225–240 V power supplies. Please note that the slot
may not be visible at first when looking at the back of the instrument.
WARNING
Risk of personal injury and material damage [W7]
If the voltage supplied to the equipment is higher than what is indicated on
the voltage selection switch, the electronics can be irreversibly damaged
and the warranty will be voided.
2. Ensure that the air vents and the cooling fan are not covered. Allow at least 15 cm (6 in)
of space around air vents for proper motor ventilation.
3. Plug the PowerLyzer 24 Homogenizer into the power supply using the appropriate power
cord supplied.
In the event of a malfunction or breakdown, grounding provides a path of least
resistance for electric current to reduce the risk of electric shock. This instrument is
equipped with an electric cord which is grounded to the chassis housing. The plug must
be plugged in to an outlet that is properly installed and grounded in accordance with all
local codes and ordinances.

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PowerLyzer 24 Homogenizer User Manual 05/2022
Note: The PowerLyzer 24 Homogenizer must be shipped in an approved packaging
crate to avoid damage which may invalidate the warranty. Packaging crates may be
purchased for a minimal charge. For additional repair and shipping inquiries, contact
QIAGEN Technical Support.
Decontamination
Should an instrument or component that has been used with radioactive or pathogenic
material require factory or field service, comply with the following procedure to ensure the
safety of service personnel.
Clean the parts to be serviced of all encrusted material and decontaminate them.
Radioactivity must not be detectable by survey equipment.
Complete the Decontamination Certification Form (see Appendix C). Please include a copy
of the Decontamination Certification Form when shipping the returned item.
If a Decontamination Certification Form is not attached, and a potential radioactive or
biological hazard is detected or suspected by QIAGEN, the equipment will not be serviced
until proper decontamination and certification is completed. The sender will be contacted for
instructions as to the disposition of the equipment. Disposition costs will be charged to the
sender.
WARNING
Hazardous chemicals and infectious agents [W10]
It is a violation of law to transport biologically hazardous or radioactive
materials without proper packaging, labeling, and appropriate
warnings.

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Operating Procedures
This section describes how to operate the PowerLyzer 24 Homogenizer system. Before
proceeding, you should familiarize yourself with the features of the PowerLyzer 24
Homogenizer by referring to page 24.
Tube motion
Due to the equipment’s design and symmetry, the 24 tubes follow the same motion. This
ensures a consistent level of lysis and homogenization for each sample. The mixture
contained in the bead beating tubes moves throughout the tubes and moves primarily along
the vertical axis to allow for efficient homogenization. Tubes must be evenly balanced on the
tube holder.
The following bead beating tubes are recommended for use with the PowerLyzer 24
Homogenizer.
Tube product name Catalog number
PowerBead Tubes, Ceramic 1.4 mm (50) 13113-50
PowerBead Tubes, Ceramic 2.8 mm (50) 13114-50
PowerBead Tubes, Glass 0.5 mm (50) 13116-50
PowerBead Tubes, Metal 2.38 mm (50) 13117-50
PowerBead Tubes, Glass 0.1 mm (50) 13118-50
PowerBead Pro Tubes, Zirconia 19301

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PowerLyzer 24 Homogenizer User Manual 05/2022
Programming
The PowerLyzer 24 Homogenizer can be programmed for speed, time, number of runs and
the length of the dwell or pause between programs. The PowerLyzer 24 Homogenizer
memory can store up to 99 custom programs, allowing for maximum repeatability and
flexibility.
Programming parameters of the PowerLyzer 24 Homogenizer are shown in the table below.
Parameter Operating range
Speed 500 to 5000 rpm in increments of 100 rpm
Time 1 s to 9 min: 59 s in increments of 1 s
Number of cycles 1 to 10
Dwell/pause between cycles Dwell minimum* up to 9 min: 59 s
Storable programs 1 to 99
* Dwell minimum varies depending upon the speed and length of cycle.
Keypad layout
The keypad of the PowerLyzer 24 Homogenizer (see diagram, next page) consists of an LCD
screen, four arrow buttons and a “RUN” and “STOP” button.

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The user can adjust four different homogenization settings with this interface. The UP and
DOWN arrow buttons are used to navigate through the menu and the LEFT and 
RIGHT arrow buttons are used to adjust parameters.
Startup of the equipment
Turn on the PowerLyzer 24 Homogenizer by pressing the ON/OFF switch located on the
rear panel near the AC power input. When the unit is turned on, a welcome screen is
displayed for 5 s showing the following information.
QIAGEN
POWERLYZER 24
rev XX/XX/XXX

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PowerLyzer 24 Homogenizer User Manual 05/2022
Active variable System status display
Program: XX CLOSE DOOR or READY TO START
S RUN SPEED rpm
T RUN TIME
C CYCLES
D CYCLE DWELL
If the run is stopped or paused, the system status will read “RUN PAUSED.”
At the end of the program, “REMOVE SAMPLES” will be displayed followed by “PROGRAM
DONE.”
WARNING
Risk of personal injury and material damage [W12]
Wait until the unit stops and displays “REMOVE SAMPLES—PROGRAM
DONE” before opening the door.

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The user can change the number of cycles using the and arrow keys. The number of
cycles ranges from 1 to 10. When the desired value is reached, the user can proceed to the
last variable, or return to program (“PROG”) to start the run.
Adjusting the pause or “dwell” between cycles
Use the and arrow keys to highlight the “D” pause/dwell option.
PROG 04 20°C
S=4000 C=02
T=0:10 D=>0:10
CYCLE DWELL
The user can change the dwell time between 2 or more cycles using the and arrow
keys. The dwell time between cycles ranges from 1 s* to 9 min: 59 s in increments of 1 s.
Once all variables have been programmed, return the cursor (>) to “PROG” and press
“Start/OK”. The screen will then display “HUBLOCK ENGAGED?”.
PROG 04 20°C
S=4000 C=02
T=0:10 D=>0:10
HUBLOCK ENGAGED?
* Dwell minimum varies depending upon the speed and length of cycle.

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Recommended settings
Sample name
and type
Bead matrix (lysing
matrix)
Setting
(speed – number of cycles x time – pause) Application
Microbial
Bacteria Zirconia 2000 – 1 x 5 min – 0 s DNA isolation
Bacteria Glass 0.1 mm 2000 – 1 x 5 min – 0 s DNA isolation
Animal
Muscle Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Brain Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Liver Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Kidney Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Lung Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Heart Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Spleen Ceramic 2.8 mm 3500 – 2 x 45 s – 30 s RNA isolation
Plant
Leaves Ceramic 2.8 mm 4200 – 2 x 45 s – 30 s RNA isolation
Leaves Metal 2.38 mm 2,000 – 1 x 3 min DNA isolation
Seeds Metal 2.38 mm 2,900 – 1 x 3 min DNA isolation
Stems Ceramic 2.8 mm 4,200 – 2 x 45 s – 30 s RNA isolation
Stems Metal 2.38 mm 2,200 – 1 x 3 min DNA isolation
Roots Ceramic 2.8 mm 4200 – 2 x 45 s – 30 s RNA isolation
Root Metal 2.38 mm 2500 – 1 x 3 min DNA isolation
Flowers Ceramic 2.8 mm 4200 – 2 x 45 s – 30 s RNA isolation
Soil
Soil Zirconia 2000 – 1 x 5 min DNA isolation
Soil Glass 0.1 mm Clay: 4000 – 1 x 45 s – 0 s
Loose, high humic: 2500 – 1 x 45 s – 0 s
DNA isolation

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PowerLyzer 24 Homogenizer User Manual 05/2022
Homogenizer speed conversion table
Use the chart below to convert FastPrep®or Precellys®homogenizing speed settings to the
equivalent PowerLyzer 24 speed setting.
PowerLyzer 24 (rpm) FastPrep 24 (m/s) Precellys 24 (rpm)
500–2400 – –
2500 4 5000
2600 – 5200
2700 – 5400
2800 4.5 5600
2900 – 5800
3000 – 6000
3100 5 6200
3200 – 6400
3300 – 6600
3400 5.5 6800
3500 – –
3600 – –
3700 6 –
3800 – –
3900 – –
4000 6.5 –
4000–5000 – –
Note: Equivalent settings slower than 2500 rpm or faster than 4000 rpm on the PowerLyzer
24 Homogenizer cannot be obtained when using the FastPrep or Precellys homogenizers.

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Maintenance Procedures
Regular maintenance
This table lists the maintenance actions to be performed by the user on a regular basis to
ensure that the PowerLyzer 24 Homogenizer functions properly. Wearing parts are shown in
the table below.
Wearing parts Part number Frequency of replacement Reason
Finger plate 13158 1 year or to destruction Necessary to maintain tubes
during homogenization
Fuse PLFUSE To destruction Fuse(s) required to function
WARNING
Electrical hazard [W13]
Potentially dangerous voltage exists inside the instrument. In order to
ensure the user’s safety, the rubber containment seal should not be
damaged, particularly during the cleaning and/or decontamination
procedures.
Replacing the fuse(s)
WARNING
Electrical hazard [W14]
Turn off the unit and unplug the power cord before replacing a fuse.
There are 2 fuses located directly below the voltage selection switch on the back of the unit.
Replace fuse by inserting a flathead screwdriver into the slot on the fuse holder, depressing,
and turning 1/4 counter clockwise. Pull out the fuse holder and inspect the fuse. If the core
wire is burnt or broken, replace the fuse. Return each fuse holder to its original location.

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Decontamination procedure
If a tube breaks when a run is in progress, decontaminate parts that may have been
contaminated with an appropriate disinfectant. The decontamination procedure is the sole
responsibility of the user. Parts that may have been contaminated can be cleaned with a
sponge or a damp cloth moistened with 10% bleach solution.
Contact QIAGEN Technical Support if additional or alternative decontamination procedures
are required to ensure the compatibility of the new procedure with the instrument.

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PowerLyzer 24 Homogenizer User Manual 05/2022
Troubleshooting Guide
General troubleshooting
Comments and suggestions
Error message: CLOSE DOOR
a) Door not closed and locked properly
Check that nothing prevents the door from closing.
Press the door down and ensure that the handle is locked properly.
b) Detection system in the door is faulty
Turn off the unit.
Contact QIAGEN Technical Support.
Fan does not work
a) No power
Check main voltage.
Check that the voltage of the unit matches that being delivered by the main
power supply.
Make sure that the unit is plugged in properly.
b) Faulty fuse
Replace fuse.
c) Faulty cooling system
Turn off the unit.
Contact QIAGEN Technical Support.
No display on the screen
a) No power
Check main voltage.
Check that the voltage of the unit matches that being delivered by the
main power supply.
Make sure that the unit is plugged in properly.
b) Faulty fuse
Check and replace fuse(s).
c) Faulty display screen
Turn off the unit.
Contact QIAGEN Technical Support.
One or several tubes not sealed tightly
Cap is not properly sealed or the tube
is faulty
If a dangerous or potentially dangerous sample is contained in the tube,
apply the proper decontamination procedure.