Homogenizer PowerLyzer 24 User manual

Sample to Insight__
May 2022
PowerLyzer®24
Homogenizer
User Manual
For lysis and homogenization of biological samples
contained in bead beating tubes at variable speeds

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PowerLyzer 24 Homogenizer User Manual 05/2022
Introduction
Before using the PowerLyzer 24 Homogenizer, it is essential you read this user manual
carefully and pay particular attention to the safety information. The instructions and safety
information in this user manual must be followed to ensure safe operation of the PowerLyzer
24 Homogenizer and to maintain the PowerLyzer 24 Homogenizer in a safe condition.
Intended use of the PowerLyzer 24 Homogenizer
The PowerLyzer 24 Homogenizer is intended for molecular biology applications. This product
is neither intended for the diagnosis, prevention, or treatment of a disease, nor has it been
validated for such use either alone or in combination with other products. Therefore, the
performance characteristics of the product for clinical use (i.e., diagnostic, prognostic,
therapeutic or blood banking) are unknown.
Requirements for PowerLyzer 24 Homogenizer users
This table covers the general level of competence and training necessary for transportation,
installation, use, maintenance and servicing of the PowerLyzer 24 Homogenizer.
Task Personnel Training and experience
Transportation No special requirements No special requirements
Installation, routine use and
maintenance
Laboratory technicians or equivalent Appropriately trained and
experienced personnel
Servicing QIAGEN Instrument Service
Specialists only
–

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General Description
The PowerLyzer 24 Homogenizer has been designed to lyse and homogenize biological
samples contained in bead beating tubes at variable speeds. It can simultaneously process
up to 24 samples in 2 ml tubes at very high speed.
Features of the PowerLyzer 24 Homogenizer system
Simultaneous homogenization of 24 x 2 ml tubes
Flexibility for any type of sample processing; broad speed range, number of cycles, time,
dwell/pause
Unique front-loading tube position for ease of use and optimal bead beating interaction
Processing of hundreds of samples per day
Up to 99 custom programmable settings
Disposable tubes to ensure no threat of cross-contamination or sample degradation
Installation Procedures
Parts
Prior to operation, remove all parts from the shipping container and inspect for damaged or
missing parts. If any parts are found to be damaged or missing, please contact QIAGEN
Technical Support.

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PowerLyzer 24 Homogenizer User Manual 05/2022
The PowerLyzer 24 Homogenizer consists of the following parts.
Description Quantity
Motor Drive Unit (115V or 230V) 1
Finger Plate (black plastic) 1
Tube Holder (blue anodized aluminum) 1
Power Cords (US, EU, UK, AUS) 1 Each
Spare Fuse 1
User Manual 1
Unpacking the PowerLyzer 24 Homogenizer
WARNING
Risk of personal injury and material damage [W6]
Do not connect the unit to the main power supply until installation is
complete and the correct voltage is selected. Do not turn the unit upside
down. The PowerLyzer 24 Homogenizer must always rest on its four feet to
avoid damaging internal components or breaking the housing.

PowerLyzer 24 Homogenizer User Manual 05/2022
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Note: Do not discard the crate and packaging foam. This packaging must be used in the
event the PowerLyzer 24 Homogenizer needs to be returned to QIAGEN for any reason.
Shipping the PowerLyzer 24 Homogenizer in any other packaging will void all warranties.
1. Using a screwdriver or power drill, remove the 16 screws around the bottom edge of the
crate.
2. Lift the lid from the crate.
3. Unscrew the two external knob screws from the back of the unit to release from the
support plate.

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PowerLyzer 24 Homogenizer User Manual 05/2022
4. Slide the PowerLyzer 24 Homogenizer away from the knob screw holes.
5. Lift the unit up and off of the crate bottom. Do not lift the unit by holding the lid.
Note: This is a heavy item: use caution when lifting to avoid back injury.

PowerLyzer 24 Homogenizer User Manual 05/2022
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CAUTION
Damage to the instrument [C1]
Do not lift the instrument by holding the door. It must be lifted by holding
the bottom of the unit. Unpack the PowerLyzer 24 Homogenizer with care
and inspect it carefully.
6. Remove the warning label and make sure the voltage is set correctly for your location
before connecting the power cord (see “Installation”, below).
Installation
WARNING
Risk of personal injury and material damage [W6]
Do not connect the unit to the main power supply until installation is
complete and the correct voltage is selected. Do not turn the unit upside
down. The PowerLyzer 24 Homogenizer must always rest on its four feet to
avoid damaging internal components or breaking the housing.

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PowerLyzer 24 Homogenizer User Manual 05/2022
1. Check that the voltage selection switch located at the back of the unit matches the
voltage delivered by your electric power supply (15 = 115 V, 30 = 230 V). Use a small
screwdriver to slide the red switch up or down to select the correct power supply. Select
“15” for 110–120 V or “30” for 225–240 V power supplies. Please note that the slot
may not be visible at first when looking at the back of the instrument.
WARNING
Risk of personal injury and material damage [W7]
If the voltage supplied to the equipment is higher than what is indicated on
the voltage selection switch, the electronics can be irreversibly damaged
and the warranty will be voided.
2. Ensure that the air vents and the cooling fan are not covered. Allow at least 15 cm (6 in)
of space around air vents for proper motor ventilation.
3. Plug the PowerLyzer 24 Homogenizer into the power supply using the appropriate power
cord supplied.
In the event of a malfunction or breakdown, grounding provides a path of least
resistance for electric current to reduce the risk of electric shock. This instrument is
equipped with an electric cord which is grounded to the chassis housing. The plug must
be plugged in to an outlet that is properly installed and grounded in accordance with all
local codes and ordinances.

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PowerLyzer 24 Homogenizer User Manual 05/2022
Note: The PowerLyzer 24 Homogenizer must be shipped in an approved packaging
crate to avoid damage which may invalidate the warranty. Packaging crates may be
purchased for a minimal charge. For additional repair and shipping inquiries, contact
QIAGEN Technical Support.
Decontamination
Should an instrument or component that has been used with radioactive or pathogenic
material require factory or field service, comply with the following procedure to ensure the
safety of service personnel.
Clean the parts to be serviced of all encrusted material and decontaminate them.
Radioactivity must not be detectable by survey equipment.
Complete the Decontamination Certification Form (see Appendix C). Please include a copy
of the Decontamination Certification Form when shipping the returned item.
If a Decontamination Certification Form is not attached, and a potential radioactive or
biological hazard is detected or suspected by QIAGEN, the equipment will not be serviced
until proper decontamination and certification is completed. The sender will be contacted for
instructions as to the disposition of the equipment. Disposition costs will be charged to the
sender.
WARNING
Hazardous chemicals and infectious agents [W10]
It is a violation of law to transport biologically hazardous or radioactive
materials without proper packaging, labeling, and appropriate
warnings.

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Operating Procedures
This section describes how to operate the PowerLyzer 24 Homogenizer system. Before
proceeding, you should familiarize yourself with the features of the PowerLyzer 24
Homogenizer by referring to page 24.
Tube motion
Due to the equipment’s design and symmetry, the 24 tubes follow the same motion. This
ensures a consistent level of lysis and homogenization for each sample. The mixture
contained in the bead beating tubes moves throughout the tubes and moves primarily along
the vertical axis to allow for efficient homogenization. Tubes must be evenly balanced on the
tube holder.
The following bead beating tubes are recommended for use with the PowerLyzer 24
Homogenizer.
Tube product name Catalog number
PowerBead Tubes, Ceramic 1.4 mm (50) 13113-50
PowerBead Tubes, Ceramic 2.8 mm (50) 13114-50
PowerBead Tubes, Glass 0.5 mm (50) 13116-50
PowerBead Tubes, Metal 2.38 mm (50) 13117-50
PowerBead Tubes, Glass 0.1 mm (50) 13118-50
PowerBead Pro Tubes, Zirconia 19301