Endress+Hauser Analytik Jena APU 28 Series User manual

Operating instructions
Sample Preparation Systems of the
APU 28 Series

Analytik Jena AG
Konrad-Zuse-Str.1
07745 Jena Germany
Phone + 49 3641 77 70
Fax + 49 3641 77 92 79
Email [e-mail]
Analytik Jena AG
Konrad-Zuse-Str. 1
07745 Jena Germany
Phone + 49 3641 77 7407
Fax + 49 3641 77 7449
Email [e-mail]
http://www.analytik-jena.com
Microsoft and Windows are registered trademarks of Microsoft Corp. The use of the
symbols ® or TM is omitted in this manual.
11-0607-001-23
02.20
Analytik Jena AG
© Copyright 2020 Analytik Jena AG
Manufacturer
Service
General Information
Copyrights and
Trademarks
Documentation number
Edition
Technical documentation
made by:

APU 28 APU 28 S APU 28 SPE Content
3
Contents
1Basic information ........................................................................................................7
1.1 User manual notes............................................................................................................ 7
1.2 Intended use ..................................................................................................................... 8
1.2.1 Purpose.............................................................................................................................. 8
1.3 Warranty and liability....................................................................................................... 9
1.4 Product identification ....................................................................................................... 9
2Safety instructions.................................................................................................... 10
2.1 Safety markings .............................................................................................................. 10
2.2 Technical condition......................................................................................................... 12
2.3 Requirements for the operating personnel................................................................... 12
2.4 Safety instructions, transport and installation.............................................................. 13
2.5 Safety instructions - operation....................................................................................... 14
2.5.1 General ............................................................................................................................ 14
2.5.2 Safety instructions - Protection against explosion and fire ......................................... 14
2.5.3 Safety instructions - electrical equipment..................................................................... 14
2.5.4 Handling of auxiliary and operating materials ............................................................. 15
2.5.5 Safety instructions: service and repair........................................................................... 15
2.6 Behavior during emergencies ........................................................................................ 16
3Function and setup................................................................................................... 17
3.1 Description ...................................................................................................................... 17
3.1.1 Device-specific features.................................................................................................. 18
3.2 Hose system .................................................................................................................... 19
3.2.1 Hose connections/hose diagrams.................................................................................. 19
3.2.2 Hose diagram APU 28 .................................................................................................... 19
3.2.3 Hose diagram APU 28 S ................................................................................................. 20
3.2.4 Hose diagram APU 28 SPE............................................................................................. 21
3.2.5 Seals and connections .................................................................................................... 22
3.3 Metering head ................................................................................................................ 22
3.4 Cannula............................................................................................................................ 23
3.4.1 Venting the sample preparation system ....................................................................... 23
4Installation and commissioning............................................................................... 25
4.1 Site requirements............................................................................................................ 25
4.1.1 Installation conditions.................................................................................................... 25
4.1.2 Space requirement.......................................................................................................... 25
4.1.3 Energy supply.................................................................................................................. 25
4.1.4 Unpacking and setting up the sample preparation system.......................................... 26
5Operation ................................................................................................................. 27
5.1 Sample enrichment AOX ................................................................................................ 27
5.1.1 Preparation ..................................................................................................................... 27
5.1.2 Loading the rack for AOX ............................................................................................... 28
5.2 Sample enrichment SPE AOX......................................................................................... 29
5.2.1Preparation ..................................................................................................................... 29
5.2.2 Loading the rack for SPE-AOX ....................................................................................... 30
5.3 Programming.................................................................................................................. 31
5.3.1 Keypad description ......................................................................................................... 31
5.3.2 Programming an AOX sequence.................................................................................... 33
5.3.3 Programming a SPE sequence (APU 28 SPE) ............................................................... 34
5.3.4 Conditioning an SPE column with methanol ................................................................ 35
5.3.5 Backwash the entire sample range................................................................................ 36
5.3.6 Setting the offset coordinates/sampler adjustment..................................................... 37
5.3.7 Pump adjustment ........................................................................................................... 39
6Maintenance and care.............................................................................................. 40
6.1 Maintenance intervals.................................................................................................... 40

Content APU 28 APU 28 S APU 28 SPE
4
6.2 Instructions......................................................................................................................40
6.2.1 Replacing the hose connections.....................................................................................40
7Troubleshooting........................................................................................................41
7.1 Malfunction of the control module................................................................................41
7.2 Equipment faults and analytical problems....................................................................41
8Transport and storage...............................................................................................42
8.1 Transport.........................................................................................................................42
8.1.1 Preparing the sample preparation unit for transport...................................................42
8.1.2 Transport notes...............................................................................................................43
8.1.3 Moving the sample preparation system inside the laboratory ....................................43
8.2 Storage.............................................................................................................................44
8.3 Recommissioning after transport or storage.................................................................44
8.3.1 Assembling and connecting the sample preparation unit after transport or storage 44
9Disposal .....................................................................................................................46
10 Specification ..............................................................................................................47
10.1 Technical data .................................................................................................................47
10.2 Standards and directives ................................................................................................48

APU 28 APU 28 S APU 28 SPE Figures
5
Figures
Figure 1 Sample preparation system – front view ........................................................... 17
Figure 2 Double channel system APU 28 S....................................................................... 18
Figure 3 Hose diagram APU 28 ......................................................................................... 19
Figure 4 Hose diagram APU 28 S ...................................................................................... 20
Figure 5 Hose diagram APU 28 SPE.................................................................................. 21
Figure 6 Fingertight screw connection ............................................................................. 22
Figure 7 Metering head (cross section)............................................................................ 22
Figure 8 Cannula ................................................................................................................ 23
Figure 9 Venting the system.............................................................................................. 24
Figure 10 Main switch on the rear of the device............................................................ 27
Figure 11 Loading pattern and example (rack AOX) ..................................................... 28
Figure 12 Ejection SPE columns ...................................................................................... 30
Figure 13 Loading pattern for SPE-AOX......................................................................... 30
Figure 14 Keypad.............................................................................................................. 31
Figure 15 Display on the control panel ........................................................................... 32
Figure 16 Display indications........................................................................................... 38
Figure 17 Dosing head not at the final depth................................................................. 38
Figure 18 Display indications........................................................................................... 39
Figure 19 Dosierkopf – finale Position............................................................................ 39
Figure 20 Display „Pump adjust“ ..................................................................................... 39

Figures APU 28 APU 28 S APU 28 SPE
6

APU 28 APU 28 S APU 28 SPE Basic information
7
1Basic information
1.1 User manual notes
The user manual provides the required know-how for the safe handling of the sample
preparation system and its components. The user manual further includes instructions for
maintaining and servicing the sample preparation system and potential causes and
remedies of any faults.
The sample preparation system must only be operated by qualified specialist personnel
instructed in preparing samples. The instruction must also include conveying the content
of this user manual and the user manuals of other system components or add-on
equipment.
Instructions for actions which occur in chronological order are numbered and combined in
action units.
Warnings are indicated by warning triangles and a signal word. The type, source and
consequences of the danger are stated together with notes on preventing the danger.
The elements of the control and analysis program are indicated as follows:
Program terms are indicated by small caps (e.g., FILE menu).
Buttons are depicted as follows (e.g. <1>)
The user manual uses the following symbols and signal words to indicate hazards or
instructions. The warnings are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause death or very serious
injuries (deformities).
CAUTION
Indicates a potentially
hazardous situation which might cause light or minor injuries.
ATTENTION
Provides information on potential material or environmental damage.
User manual
Requirements to be met
by the user
Conventions
Symbols and signal words
used

Basic information APU 28 APU 28 S APU 28 SPE
8
1.2 Intended use
The sample preparation systems APU 28, APU 28 S and APU 28 SPE must only be used for
the sample preparation methods described in this user manual.
Any other use is not as intended!
Only the operator is liable for any damages that result from this.
Operational safety can only be guaranteed if the device is used in accordance with the
information in this user manual.
The intended use also includes the adherence to the installation conditions prescribed by
Analytik Jena AG which are available from the customer service address stated above.
1.2.1 Purpose
The APU 28 series consists of 6 sample preparation systems – 3 standard systems
conceived for working with AOX columns produced by Analytik Jena AG (APU 28,
APU 28 S and APU 28 SPE) and 3 flexible systems (APU 28 flexi, APU 28 S flexi and
APU 28 SPE flexi) which are conceived for the use of alternative AOX columns (40 x 9 mm
or 47 x 6 mm).
Operation, maintenance and servicing of the flexi systems do not differ from the
corresponding standard systems. Therefore, the following document does not contain
separate information for these systems.
Standard systems are not suitable to be used with alternative AOX tubes while flexi
systems are not suitable to be used with Analytik Jena columns. If required, the systems
can be upgraded with a suitable auxiliary tray.
The sample preparation system APU 28 is a fully automated single channel system
for preparing samples for the AOX determination according to the column method
defined in ISO 9562.
The individual samples are processed in sequential order.
The sample preparation system APU 28 S is a fully automated double channel system
for preparing samples for the AOX determination according to the column method
defined in ISO 9562.
Two samples are processed simultaneously in one step.
Compared to the APU 28 system, this translates into twice the sample throughput.
The sample preparation system APU 28 SPE is a fully automated single channel system
for preparing samples for the SPE-AOX determination and the AOX determination in
accordance with ISO 9562.
The individual samples are processed in sequential order.
APU 28
APU 28 S
APU 28 SPE

APU 28 APU 28 S APU 28 SPE Basic information
9
1.3 Warranty and liability
The warranty duration and liability comply with the legal requirements and the provisions
in the general terms and conditions of Analytik Jena AG.
Deviations from the intended use described in this user manual result in limitations of
warranty and liability in the event of a damage. Damage to wearing parts is not included
in the warranty.
Warranty and liability claims are excluded for personal injury and property damage due to
one or several of the following causes:
use of sample preparation systems other than intended
improper commissioning, operation and servicing
modifications without prior consultation with Analytik Jena AG
unauthorized intervention
operation of the sample preparation system with faulty safety equipment or
improperly fitted safety and protection equipment
inadequate monitoring of the equipment components subject to wear
use of other than original spare parts, wearing parts or consumables
improper repairs
faults due to the non-observance of this user manual
1.4 Product identification
The type plate contains the following information on the sample preparation system:
Manufacturer identification
Model name
Model number
Serial number
Year of manufacture
Information on conformity
Type plate

Safety instructions APU 28 APU 28 S APU 28 SPE
10
2Safety instructions
For your own safety and to ensure error-free and safe operation of the sample
preparation system, please read this chapter carefully before commissioning.
Comply with all safety instructions contained in this user manual and pay careful attention
to all messages and notes which are displayed on the display of the control unit.
2.1 Safety markings
Safety symbols have been attached to the sample preparation system and accessories
whose content must always be observed.
Damaged or missing safety symbols can cause incorrect actions leading to personal injury
or material damage! The safety symbols must not be removed! Damaged safety symbols
must be replaced without delay!
The following safety symbols are attached to the sample preparation system and its
accessories:
Warning of
electric voltage
Warning of hand injuries
Warning against
corrosive substances
Warning of cuts
Warning of
hand injuries
in gear drive
Unplug power cord
Follow instructions
Warning symbol
Mandatory sign

APU 28 APU 28 S APU 28 SPE Safety instructions
11
The reservoir for sodium nitrate rinse solution with nitric acidity (pH ≈2) and the waste
container are marked with the hazard pictogram for corrosive substances:
Warning against
corrosive substances
The storage bottle for methanol is marked with the following warning symbols:
Highly flammable
Toxic
Health hazard
The device contains regulated substances (according to the directive "Management
Methods for the Restriction of the Use of Hazardous Substances in Electrical and
Electronic Products"). Analytik Jena AG guarantees that the substances will not leak within
the next 25 years and, therefore, will not cause any environmental danger or health risk
within this period of time if the device is used as intended.
China RoHS label
GHS pictogram
GHS pictogram
EFUP pictogram

Safety instructions APU 28 APU 28 S APU 28 SPE
12
2.2 Technical condition
Design and construction of the sample preparation unit correspond to the current
generally accepted rules of technology. Unauthorized modifications or changes, especially
such that affect the safety of the staff and the environment, are generally not allowed.
The following has to be observed:
Any manipulation of the safety equipment is prohibited! In case of an accident
manipulations of the safety equipment will be interpreted as deliberate!
The sample preparation unit must only be operated in sound and operationally safe
condition. The technical condition must always comply with the legal requirements
and regulations.
Prior to every use, the sample preparation unit must be checked for damage and sound
condition.
Any changes to the sample preparation system affecting its safety must be reported by
the operating personnel to the operator without delay.
The equipment components must only be connected to supply cables intended and
designed for this purpose.
All safety equipment and interlocks must be well accessible and regularly checked for
proper operation.
2.3 Requirements for the operating personnel
The APU 28 must only be operated by qualified specialist personnel instructed in
preparing samples. The instruction must also include conveying the content of this user
manual and the user manuals of other system components or add-on equipment.
When used by untrained personnel or in an improper manner or for improper use, the
sample preparation unit can pose a risk.
Therefore, every person tasked with the operation of the sample preparation unit must
have read and understood this user manual and the user manuals of any additional
equipment before carrying out the respective tasks. This also applies if the respective
person has already worked with or been trained on this kind of sample preparation unit.
It is recommended that the operator have the operating personnel confirm the knowledge
of the content of the user manual in writing. The ultimate responsibility for the accident-
free operation of the sample preparation unit rests with the operator or the specialist
personnel authorized by him.
In addition to the safety at work instructions in this user manual the generally applicable
safety and accident prevention regulations of the respective country of operation must be
observed and adhered to. The operator must ascertain the latest version of these
regulations.
The user manual must be accessible to the operating and service personnel at any time!

APU 28 APU 28 S APU 28 SPE Safety instructions
13
The following has to be observed:
The sample preparation unit must only be commissioned, operated and serviced by
trained personnel instructed in technical safety.
The operation or servicing of the sample preparation unit by minors or individuals
under the influence of alcohol, drugs or medication is not permitted.
It must be ensured that only authorized personnel works with the sample preparation
unit.
The operating personnel must be familiar with the dangers arising from measuring
liquids. The appropriate protective equipment must be used.
Prior to pauses or at the end of the work appropriate skin cleaning and protection
measures must be carried out.
Eating, drinking, smoking or handling open flames is prohibited in the operating room
of the sample preparation unit!
2.4 Safety instructions, transport and installation
The APU 28 is always installed by the customer service department of Analytik Jena AG or
its authorized and trained specialist personnel.
Unauthorized assembly and installation are not permitted. Incorrect installation can
create serious hazards.
The following has to be observed:
When placing the device on the laboratory desk consider the movement range of the
arm during operation. Ensure that the whole potential movement range behind the
device is unobstructed.
Insufficiently secured components pose a risk of injury!
During transport the components of the equipment must be secured in accordance
with the instructions in the user manual.
Only transport the sample preparation unit in its original packaging!
Ensure that all transport locks and safety devices have been fitted and that the sample
preparation unit is completely empty.
To prevent health damage the following must be observed when moving the sample
preparation unit in the laboratory (lifting and carrying):
For safety reasons, two persons are required to transport the sample preparation unit who
must hold the unit by either side of the equipment.
Because the sample preparation unit does not have carrying handles, hold the device
firmly with both hands by its lower end.
The guide values and statutory limits for lifting and carrying loads without auxiliary
equipment must be observed and adhered to.

Safety instructions APU 28 APU 28 S APU 28 SPE
14
2.5 Safety instructions - operation
2.5.1 General
Before starting the sample preparation unit, the operator must verify the proper condition
of the sample preparation unit including its safety equipment. This is particularly
important each time the sample preparation system was amended, upgraded or repaired.
The following has to be observed:
The sample preparation unit must only be operated if all protective equipment (e.g.
covers, drip pans for chemicals) are in place, properly installed and fully operational.
The sound condition of the protection and safety equipment must be checked
regularly. Any defects must be corrected as soon as they occur.
Protective and safety equipment must never be removed, modified or switched off
during operation.
Caution near the movement range of the arm guiding the cannula! Accident risk during
operation!
Free access to the power switch on the back of the enclosure and to the mains power
cord has to be ensured during operation.
2.5.2 Safety instructions - Protection against explosion and fire
The sample preparation unit must not be operated in an explosive environment. Smoking
or handling open flames is prohibited in the room in which the sample preparation unit is
operated!
The operating personnel must be familiar with the location of the fire-fighting equipment
in the operating room of the sample preparation unit.
2.5.3 Safety instructions - electrical equipment
Work on electrical components of the sample preparation unit may only be carried out by
a qualified electrician in accordance with the applicable electrical engineering rules.
The following has to be observed:
The sample preparation unit must always be switched off before extension modules
are electrically connected or disconnected.
Before opening the sample preparation unit, it must be switched off using its main
switch and the mains connector must be disconnected from the power outlet!
The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty or damaged cables, must be repaired without
delay.
Ensure that no liquid reaches the cable connections or the interior of the electrical
device! Danger of electric shock!
Immediately switch off the sample preparation unit using the main switch (on the rear
of the housing) and pull the mains connector from the power outlet if there is any
interference with the electric components.

APU 28 APU 28 S APU 28 SPE Safety instructions
15
Behavior after restoration of power supply (after a power failure):
The device will perform a number of automatic movements (initialization) after the
power supply was restored. Therefore, the operator must take particular care within
the operating radius of the arm guiding the cannula! Risk of accidents!
2.5.4 Handling of auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well as
for their safe handling. This is particularly important for radioactive, infectious, poisonous,
corrosive, combustible, explosive and otherwise dangerous substances.
When handling dangerous substances local safety codes and guidelines must be observed.
The following general notes do not replace the specific local regulations or the regulations
in the EC safety data sheets of the manufacturers for the auxiliary and operating
materials.
The following has to be observed:
The relevant regulations and the notes in the EC safety data sheets of the
manufacturers have to be observed and complied with regards to storage, handing,
use and disposal for all auxiliary and operation materials used during the operation or
maintenance of the sample preparation unit.
Auxiliary and operation materials may never be placed in containers or vessels for
food. The approved containers for the relevant material are to be used and these have
to be labeled accordingly. The notes on the labels have to be observed!
Wearing protective goggles and rubber gloves is mandatory when handing reagents.
The notes on the labels have to be observed.
The regulations and notes on the safety data sheets for the handling of sodium nitrate
solution and methanol must be observed!
Biological samples must be handled according to local guidelines regarding the
handling of infectious material.
Caution when handling glass components. Risk of broken glass and therefore risk of
injury!
Auxiliary and operating materials as well as their containers may not be disposed in
domestic waste or enter the sewage system or the soil. The applicable regulations for
disposal of these materials must be meticulously observed.
Ensure good room ventilation in working rooms.
2.5.5 Safety instructions: service and repair
Maintenance of the sample preparation unit is generally performed by the customer
service department of Analytik Jena AG or its authorized and trained specialist personnel.
Unauthorized servicing can lead to maladjustment or damage to the sample preparation
unit. Therefore, the tasks that the operator is allowed to carry out are strictly limited to
the tasks listed in chapter →"Maintenance and care" on page 40.
The following has to be observed:
The exterior of the sample preparation unit may only be cleaned with a damp, not
dripping, cloth after the unit has been switched off.

Safety instructions APU 28 APU 28 S APU 28 SPE
16
The sample preparation unit must always be switched-off before carrying out any
service and repair work (unless stated otherwise).
Only use original accessories and original replacement parts from Analytik Jena AG.
The notes in the chapter "Service and care" must be observed.
All protective equipment must be reinstalled correctly immediately after completion of
the service and repair work and be checked for operation!
2.6 Behavior during emergencies
The sample preparation unit must be switched off using the main switch (on the
equipment backplate) and disconnected from the mains supply in case of dangerous
situations or accidents.
Because a rapid response can save lives during an emergency, the following has to be
ensured:
The operating staff has to be aware where safety equipment, accident and danger
alarms as well as first aid and rescue equipment are located and be familiar with their
handling.
The operator is responsible for the respective training of the operating staff.
All equipment for first aid (first-aid kit, eyewash bottles, stretcher, etc.) as well as
equipment for firefighting (fire extinguishers) must be within reach and easy to access.
All equipment has to be in a sound condition and should be checked regularly.

APU 28 APU 28 S APU 28 SPE Function and setup
17
3Function and setup
3.1 Description
Figure 1 Sample preparation system – front view
1 Pump unit
2 Warning label, other versions →"Safety markings" page 10
3 Cannula guide
4 Sample tray
5 Waste hose
(to the waste container/canister) positioning instructions relevant to the unit's functioning
→"ATTENTION" pages 27 and 29
6 Control unit with display and keypad →"Programming" page 31
7 Basic device
8 Storage container for nitrate solution
9 Position 28 (scraper SPE columns)
10 Storage bottle for methanol (only APU 28 SPE)
11 Rack with 4 feet
12 Circular spirit level →"Unpacking and setting up the sample preparation system" page 26

Function and setup APU 28 APU 28 S APU 28 SPE
18
The standard version of the sample preparation system APU 28 consists of the following
components:
Basic device including power supply
Control unit – used for programming sequences, controls the running gear and the
pump unit
Each of the three sample preparation systems APU 28, APU 28 S and APU 28 SPE is
delivered with one tray and its accessories.
Before the initial start-up of the unit, the operator must align the running gear with the
tray or verify the alignment. The offline coordinates are stored in the control unit which
means that the control units and the trays of two different systems must not be mixed up.
The maximum sample container volume is 120 ml.
AOX columns and sample containers must always be positioned in accordance with the
loading plan supplied with the unit. Columns must be fastened by rotating them by
30 degrees (locking device).
3.1.1 Device-specific features
The sample preparation system APU 28 S is a double channel system which allows
processing two samples at the same time.
Figure 2 Double channel system APU 28 S
Additional accessories to the sample preparation system APU 28 SPE are a scraper, a
receptacle and 12 inserts for the SPE columns.
Basic device
Control unit
Sample cup
APU 28 S
APU 28 SPE

APU 28 APU 28 S APU 28 SPE Function and setup
19
The scraper for the SPE columns is placed in position 28 and the receptacle is placed
underneath this position; for further information →page 30.
3.2 Hose system
3.2.1 Hose connections/hose diagrams
The connection between the individual components is made with labeled hoses. The
encircled numbers and letters in the hose diagram correspond to the labels on the hoses
in the sample preparation system.
To prevent carry-over, hoses and hose connections are flushed automatically →
"Backwash the entire sample range" page 36.
The effectiveness of this function can only be guaranteed when using hoses supplied by
Analytik Jena AG.
Please use the following hose numbers for replenishment orders.
3.2.2 Hose diagram APU 28
Figure 3 Hose diagram APU 28
Hose diagram

Function and setup APU 28 APU 28 S APU 28 SPE
20
3.2.3 Hose diagram APU 28 S
Figure 4 Hose diagram APU 28 S