Analytik Jena aj CYBERTRON InnuPure C12 User manual

InnuPure C12
Extraction system

Manufactured by:
AJ Cybertron GmbH
Louis-Blériot-Str. 5
12487 Berlin
Germany
Service: Analytik Jena AG
Kundendienst
Konrad-Zuse-Str. 1
07745 Jena
Germany
Phone: Hotline: + 49 (0) 3641 / 77 94 00
Fax: + 49 (0) 3641 / 77 76 77 76
E-Mail: [e-mail]
[e-mail]
General information about Analytik Jena AG
on the internet: http://www.bio.analytik-jena.de
Copyrights and Trademarks
Microsoft, Windows 2000 / XP / Vista, MS Excel are registered trademarks of Microsoft Corp.
Publication No.: 9332:002.23
Edition – January 2011
Technical documentation made by:
Analytik Jena AG
This documentation describes the state of this product at the time of publishing. It
need not necessarily agree with future versions of the product.
Subject to change!
© Copyright 2011 Analytik Jena AG

Contents
InnuPure C12 Issue 01/2011 1
Contents
1Introduction .....................................................................3
1.1 Use of the InnuPure C12.......................................................................3
1.2 Notes on the use of this manual ...........................................................3
2Safety Instructions ..........................................................5
2.1 Warning and safety symbols on the InnuPure C12 ..............................5
2.2 General warning information.................................................................5
3Device description and functional principle .................8
3.1 Construction and connections...............................................................8
3.2 Keypad functions...................................................................................9
3.3 Functional principle .............................................................................10
4Installation, transport, storage conditions..................13
5Commissioning and powering up................................15
5.1 Connecting and powering up / powering down ...................................15
5.2 Assembly instruction Priming Station..................................................16
5.3 Preparation of buffers and solutions ...................................................16
5.4 Preparation of the sample tray............................................................17
5.5 Position of the sample tray..................................................................18
6Packaging instruction...................................................19
7Operation .......................................................................22
7.1 Preparing and starting an isolation routine .........................................22
7.2 Software functions...............................................................................22
7.3 InnuPure C12 tools .............................................................................23
7.3.1Initialization..........................................................................................23
7.3.2 Waste tips............................................................................................24
7.3.3 Remove sample tray ...........................................................................25
7.3.4 Move magnets out...............................................................................25
7.4 Performance of an isolation routine ....................................................26
7.5 Cancel or continue a running program................................................27
8Up-date of the InnuPure C12 ........................................29
8.1 Installation of UpDateIP ......................................................................29
8.1.1 System requirements ..........................................................................29
8.1.2 Installation procedure..........................................................................29
8.2 Starting and finishing UpDateIP ..........................................................29
8.3 Language versions..............................................................................30
8.4 Help .....................................................................................................30

Contents
2 Issue 01/2011 InnuPure C12
8.5 The UpDateIP work place ...................................................................30
8.5.1 Menu bar .............................................................................................32
8.5.2 Toolbar and function buttons ..............................................................32
8.6 Loading of isolation protocols from PC ...............................................33
8.7 Download of isolation protocols from InnuPure C12...........................33
8.8 Transfer of isolation protocols .............................................................33
8.8.1 Copy isolation protocols ......................................................................34
8.8.2 Move isolation protocols......................................................................34
8.9 Up-load of isolation protocols to InnuPure C12 ..................................34
8.10 Delete isolation protocols ....................................................................35
9Care, maintenance ........................................................37
9.1 Cleaning ..............................................................................................37
9.2 Changing fuses ...................................................................................38
10 Waste disposal ..............................................................39
11 Technical data ...............................................................41
12 Table of figures..............................................................43
13 EC Declaration of Conformity ......................................45

Introduction
Use of the InnuPure C12
InnuPure C12 Issue 01/2011 3
1 Introduction
1.1 Use of the InnuPure C12
The InnuPure C12 is a flexible and efficient extraction system for the completely
automated isolation and purification of nucleic acids. The system, which was
developed and produced in Germany, is designed for small and medium sample
throughput. A wide range of starting materials can be processed. The system
combines a unique way of performing liquid handling steps with an extremely
fast walk-away principle.
The InnuPure C12 is equipped with pre-installed application programs to
eliminate time-consuming programming. The high degree of flexibility of
InnuPure C12 allows the isolation of DNA or RNA from up to twelve samples in
parallel. The work intensive steps for sample lysis, that were often necessary up
to now are now incorporated into the automated extraction process (depending
on the starting material).
The nucleic acids to be isolated are adsorbed to surface-functionalized
magnetic particles. The required extraction chemistry is optimally adapted to the
application at hand and facilitates the isolation of very pure nucleic acids with
excellent yields.
The extraction principle effectively prevents cross contamination, a problem that
frequently occurs in vacuum-based extraction methods. The isolated nucleic
acids can be used immediately afterwards for downstream applications.
Extraction kits optimized for InnuPure C12 are available for the isolation of
genomic DNA, cellular RNA, viral or bacterial nucleic acids, as well as the
processing of forensic samples. The extraction process requires between 20
and 40 minutes, depending upon application.
1.2 Notes on the use of this manual
For easy reference and orientation, the manual uses the following warning and
information symbols as well as the shown methodology:
Danger! Dangerous touch voltage!
Caution!
This information must be observed under all circumstances to prevent
personal damage.
Attention!
This information must be observed to avoid damage to the device.
Disconnect mains plug!

Introduction
Notes on the use of this manual
4 Issue 01/2011 InnuPure C12
Formatted
text
In the description of the operating steps, menu commands, dialog boxes,
buttons and options, etc. are formatted in bold characters.
Menu commands of a command sequence are separated by a slash ( / ), e.g.
File / Open.
Buttons are marked by square brackets, e.g. [Save].
→
Cross-reference to other sections and illustrations (italic).
Note
Observe the notes marked in this way to ensure correct function of the device
and to avoid operating errors for obtaining correct results.
The following systematic approach is introduced in the manual:
•The chapters and figures are numbered consecutively.
•Each image has its own caption.
•A cross reference is indicated with an arrow (e.g. →"Information on
the use of this manual" p. 3).
•Working steps are numbered.

Safety Instructions
Warning and safety symbols on the InnuPure C12
InnuPure C12 Issue 01/2011 5
2 Safety Instructions
Read through this chapter carefully prior to commissioning the InnuPure C12 for
your own safety and for trouble-free operation.
Follow all the safety instructions explained in the manual, as well as all
messages and information displayed on the screen by the control firmware.
2.1 Warning and safety symbols on the InnuPure C12
The following warning and safety symbols are affixed on the InnuPure C12:
Disconnect mains plug!
This symbol is affixed on the back side of the device next to the mains input.
Electronic scrap! Dispose the device not into the domestic waste!
At the end of its service life, the InnuPure C12 and all its electronic components
must be disposed of as electronic scrap in accordance with valid regulations.
This Symbol is on the rear side of device.
2.2 General warning information
Proper use!
The InnuPure C12 including its original accessories may only be used for the
applications described in this instruction manual. No liability can be accepted by
the manufacturer for any other application, also for any individual component
groups or individual parts.
Repairs only from authorised personnel!
The manufacturer accepts no liability for service or repair work not carried out
by authorised service personnel. All warranty claims become null and void.
Local regulations!
Observe the local safety regulations applicable for the operation of the device
(e.g. occupational safety regulations, accident prevention regulations, accident
protection regulations).
References to possible hazards in the manual do not replace the local safety
regulations to be observed.
Personnel!
The InnuPure C12 may only be operated by qualified personnel.
Knowledge gained from this manual is absolutely essential and serves as a
prerequisite.

Safety Instructions
General warning information
6 Issue 01/2011 InnuPure C12
Shutdown in case of an emergency!
In an emergency the InnuPure C12 is to be isolated from the mains by
disconnecting the plug from the mains socket.
Make sure that the mains plug is easily accessible.
Electric shock!
The InnuPure C12 is supplied with electrical power. Extremely dangerous
electrical voltages occur at various points in the system!
The mains plug may only be connected to sockets with an earthing contact
such that Protection Class I (earthing connection) is ensured for the device. The
protective effect must not be nullified by using an extension cable without an
earthing connector.
Disconnect the mains plug before opening the device! The device hood may
only be opened by the Technical Customer Service department of Analytik Jena
AG or by trained personnel!
Before connecting the InnuPure C12 to the mains supply, check that the
operating voltage on the rating plate on the left side of the device agrees with
the mains voltage at the intended socket. Operation with any other operating
voltage than that specified can lead to the destruction of the device.
Only fuses of the specified type may be used.
Caution condensed water!
In case of large differences between the storage and installation temperatures,
before connecting wait until the InnuPure C12 has attained the ambient
temperature to avoid damage to the device caused by condensed water.
Operating substances, dangerous substances
The operator is responsible for the selection of substances used in the process
as well as for their safe handling. This is particularly important for radioactive,
infectious, poisonous, corrosive, combustible, explosive and otherwise
dangerous substances.
When handling dangerous substances local safety instructions and guidelines
must be observed.
Warnings on the labels must be always observed.
Only use clearly marked containers as well as protective goggles and rubber
gloves.
Biological samples have to be handled according to local guidelines regarding
the handling of infectious material.
Cleaning and decontamination measures
The operator is responsible for carrying out suitable
decontamination should the
device be contaminated externally or internally with dangerous substances.
Spots, drops or larger spillages should be removed and cleaned using an
absorbent material such as cotton wool, laboratory wiping cloths or cellulose.
The affected areas are then to be wiped with an Incidin Plus solution,
Decosept AF spray disinfectant (Dr. Schuhmacher GmbH) or Meliseptol HBV
– cloths (B. Braun).
.

Safety Instructions
General warning information
InnuPure C12 Issue 01/2011 7
Before another cleaning or decontamination procedure is used as that
prescribed by the manufacturer, the user shall check with the manufacturer that
the intended procedure will not damage the device.
Transportation!
In case of transportation only use the provided packaging and follow the
packaging instruction strictly. The weight of the InnuPure C12 is approx.
28.5 kg. Thus never bear up the device alone!
Before transportation remove all samples, reagents and the sample tray out of
the device.
No operation in areas subject to a risk of explosion!
Heat build-up
A heat build-up can cause overheating and faults in the device.
Ensure that the ventilation slits on the InnuPure C12 are kept free at all times!
Water
Ensure that no liquid can enter the InnuPure C12.
The device could be damaged.
Do not place any containers with liquids on the device!
Corrosion risk
Do not place the device in the immediate vicinity of aggressive vapours, e.g.
strongly corrosive acid or alkali vapours. The vapours could corrode the
connections and mechanical parts of the device.
Danger of tilting the InnuPure C12
The components of InnuPure C12 are connected with the interfaces of the
device via plug connections. In case of tilting device the disconnection of a
component could take place and the proper function of device can't be
guaranteed.
Device could be damaged!
Caution!
Do not run the InnuPure C12 with open front door! The door should be only
opened in case of any cleaning purpose!
Nickel allergy!
The sample tray is coated by nickel.

Device description and functional principle
Construction and connections
8 Issue 01/2011 InnuPure C12
3 Device description and functional principle
3.1 Construction and connections
Front view
1 Status LED's
2 Display
3 Switch for sample
compartment light
4 Front door
5 Sample tray
6 Admission of sample
Tray
Fig. 3-1 Front view of the InnuPure C12
1 Ceramic pistons
2 Removing tips
3 Tip adapter
4 Sample tray
Fig. 3-2 View inside of InnuPure C12
Via the admission (6 in Fig. 3-1) of the sample tray (5 in Fig. 3-1) in the front
door (4 in Fig. 3-1), all necessary pre-filled strips (containing all buffer
solutions), the tips, the Elution Tubes and the samples are placed to the right
position inside the device. The sample tray is moved inside the InnuPure C12,
by what the tips and the solutions can be taken up in the right order. Thereby
the sequence and volumes of the single pre-filled buffers and the sample are
depending on the isolation protocol, which is installed on the InnuPure C12.

Device description and functional principle
Keypad functions
InnuPure C12 Issue 01/2011 9
The InnuPure C12 is a stand alone device without the necessity of a PC with
control software. New isolation programs can be installed on the device by a
simple update tool as described in section "Up-date of the InnuPure C12" on
page 29. Therefore a PC is needed.
A green and a red LED status indicators (1 in Fig. 3-1) are mounted beside the
display (2) of the InnuPure C12 to show the active and working status of the
device.
Connections and switches on the back side of the device
1 Ventilation slit
2 Mains switch
3 Fuse holder
4 Mains connection
5 RS 232 interface
Fig. 3-3 Connections on the back side of the InnuPure C12
3.2 Keypad functions
Fig. 3-4 Keypad functions

Device description and functional principle
Functional principle
10 Issue 01/2011 InnuPure C12
The keys are configured with the following functions:
LED (red and
green)
Displays status of the device


Move the cursors to a certain position on the display.
ENTER / Start Confirm an entry.
Start a chosen isolation protocol or an option of the
tool list.
ESC / Stop Abort an isolation protocol in progress.
Return to superordinate menu level.
3.3 Functional principle
Lysis
In dependence on the used starting material or type of starting material, the
lysis is processed inside of the device or has to be performed manually outside
of the device.
The lysis routine for each starting material is described in detail in the manual of
the extraction kit.
Lysis outside the device Lysis inside the device
Fig. 3-5 Lysis of the starting material
The MAG-Suspension, which contains the magnetic particles, is added to the
starting material. Thereby it is really important, that the MAG – Suspension is
homogenized well (by using a vortexer) to get the right amount of magnetic
particles inside the sample.
Sample processing
After starting the isolation protocol, the InnuPure C12 is taking the provided
MFR-Tips from the accordant row of the sample tray.
The mixture of the lysed sample and the MAG-Suspension is homogenized by
up and down pipetting, followed by the transfer of the solution to the Binding
Buffer.

Device description and functional principle
Functional principle
InnuPure C12 Issue 01/2011 11
Due to the Binding Buffer, the nucleic acids are bound to the magnetic particles.
During the next step a bar magnet enters the plastic insert within the MFR –
Tips, by what the magnetic particles with the bound nucleic acid will be fixed on
the insert in consequence of the magnetic field. Beside this, the contamination
of the bar magnets could be restricted completely by the plastic cover.
Fig. 3-6 Sample processing
Washing
The magnetic particles with the bound nucleic acid are moved to the next tubes.
By up and down pipetting of different washing buffers the nucleic acid is
purified.
Thereby the number, the amount and the kind of washing buffers is depending
on the type of used starting material. Additional the buffers differs in case of
DNA or RNA isolation.
Fig. 3-7 Washing of the nucleic acids
Removing of Ethanol
A special Ethanol Removing Buffer ensures by up and down pipetting the
complete elimination of ethanol traces on the magnetic particles and thus inside
the nucleic acid.
Fig. 3-8 Removing of Ethanol

Device description and functional principle
Functional principle
12 Issue 01/2011 InnuPure C12
Elution
The last tube contains the Elution Buffer. The magnetic particles are left inside
the tube, the nucleic acid is eluted in a defined volume and the magnetic
particles are removed again from the sample.
The nucleic acid is now purified, pipetted to the final Elution Tube and ready for
other down-stream applications.
Fig. 3-9 Elution of DNA/RNA

Installation, transport, storage conditions
InnuPure C12 Issue 01/2011 13
4 Installation, transport, storage conditions
Installation conditions
Attention! Observe the following installation conditions under all
circumstances!
Keep the ventilation slits on the back side of device free!
Do not place any objects on the device!
No operation in areas subject to a risk of explosion!
No aggressive vapours, e.g. highly corrosive acids or alkalis in the immediate
vicinity of the device.
The installation site must satisfy the following conditions:
•The InnuPure C12 site of operation must be free of draughts, dust,
corrosive vapours and vibrations.
•Do not install the InnuPure C12 in the vicinity of electromagnetic
fields (e.g. motors).
•Avoid water drips, splashes and spray close to the InnuPure C12.
•Do not expose the InnuPure C12 to direct sunlight or radiation from
heaters.
•Temperature range in operation: + 5 ... + 40 °C
•Humidity in operation: up to 80 % (at + 30 °C)
Place requirements
The dimensions of the InnuPure C12 are displayed in the following:
•Closed device (W x D x H): 380 mm x 530 mm x 370 mm
•With open front door (W x D x H): 380 mm x 920 mm x 370 mm
•Sample tray (W x D X H): 200 mm x 250 mm x 23 mm
•Priming station (W x D x H): 210 mm x 260 mm x 160 mm
The pre-loaded tray has to be moved to the device via the front slit. Additional
the door of the InnuPure C 12 must be opened for cleaning purposes (
"Cleaning" p. 37) and has to be added to the dimensions of the device.
The shortest distance between the ventilation slits and the nearest object is 50
cm to guarantee the air exchange of device and environment. Do not place
device on a soft surface.

Installation, transport, storage conditions
14 Issue 01/2011 InnuPure C12
Conditions for storage and transport
The following environment conditions must be observed for transport and
storage:
•Temperature range: -40...+70 °C
•Humidity: up to 80 %
Note
Before transportation and storage of the device, the sample tray has to be
emptied from all consumables to prevent any device damages.

Commissioning and powering up
Connecting and powering up / powering down
InnuPure C12 Issue 01/2011 15
5 Commissioning and powering up
5.1 Connecting and powering up / powering down
Electric shock!
Before connecting the InnuPure C12 to the mains supply, check that the
operating voltage on the power sign on the device agrees with the mains
voltage at the intended socket. Operation with any other operating voltage than
that specified can lead to the destruction of the device.
Caution condensed water!
In case of large differences between the storage and installation temperatures,
wait until the InnuPure has attained the ambient temperature before connecting
to avoid damage to the device caused by condensed water.
1. Remove the InnuPure C12 from its packaging
2. Connect the mains cable to the connector on the back side of the device
(Fig. 3-3 S. 9)
3. Switch on InnuPure C12 at the main switch (backside of the device). The
InnuPure C12 now will be initialized automatically.
4. After the first screen appears wait until a beep is audible.
5. Press [ENTER/Start] after the start-up screen appears to enter the main
menu of the InnuPure C12. The InnuPure C12 is now ready for operation.
Fig. 5-1 Main menu
Powering down
At the end of the isolation protocol you can power down the InnuPure C12 at
the mains switch.

Commissioning and powering up
Assembly instruction Priming Station
16 Issue 01/2011 InnuPure C12
5.2 Assembly instruction Priming Station
Position Description Quantity
1 Screws 1
2 Side panel 2
3 Cross brace 3
4 Baseplate 4
5 Allen key 5
Fig. 5-2 Assembly instruction Priming Station
5.3 Preparation of buffers and solutions
All buffers and solutions are provided in pre-filled and sealed strips regarding to
the isolation routine and starting material. The preparation of sample tray is
described in detail in each Kit manual and in section "Preparation of the sample
tray" on page 16.
Follow the instruction shown in the initial steps of the Kit components within the
Kit manual.
It is also recommended to pre-heat the used thermo mixer for special lysis steps
according to the Kit manual, shown in point "Recommended steps before
starting".

Commissioning and powering up
Preparation of the sample tray
InnuPure C12 Issue 01/2011 17
5.4 Preparation of the sample tray
Nickel allergy!
The sample tray is coated by nickel.
1, 3 Reagent Strip holder
2 Pre-filled Reagent Strips
4, 6 Clamp of the lock
5 Waste Box for MFR filter tips
7 MFR filter tips
8 Elution Tubes
Fig. 1 Sample Tray
Loading of sample tray
Before an extraction process can be started, the tray has to be pre-loaded with
additional equipment, as shown below or described in detail in the user manual
of each special purification / isolation kit.
1. Place the Waste Box into the Sample Tray.
2. Position the Reagent Strips inside the slots of the Sample Tray. Legen Sie
die Reagenzien-Strips in die Vertiefungen des Proben-Trays.
Note:
The label "AJ" on the Reagent Strip has to be positioned torwards to the
Waste Box.
Fig. 2 Correct position of the Reagent Strips inside the Sample Holder.

Commissioning and powering up
Position of the sample tray
18 Issue 01/2011 InnuPure C12
3. Press the clamps of the Strip Holder together, flap it down so that the ends
of the Reagent Strips are covered and release the clamps.
The Strip Holders are locked correctly if the pins at the ends of the Strip
Holders are latched in the holes of the Sample Tray and the red dot next to
the clamps are covered again.
Fig. 3 Lock the Strip Holders
4. Place the MFR filter tips and the Elution Tubes into the Sample Tray
Note:
The correct order of the Reagent Strips, MFR filter tips, Elution Tubes is
depending on the application (please refer to the description in the user
manual of the kit).
5.5 Position of the sample tray
The pre-loaded sample tray ("Preparation of the sample tray" p. 17) has to be
pushed to the device, so that the Elution Tubes are in the front of the device.
The tray has to be moved into the InnuPure C12 until perceiving a slight
resistance.
On pressing against this resistance, the tray will be drawn in. On completion of
an extraction routine, the sample tray with the consumables automatically
moves out again.
The initialization ("Initialization" p. 23") and movement of the sample tray to
the correct position inside the device is done automatically by the InnuPure
C12.