HP Compaq d300 Series User manual

HP D300 Digital Dispenser
Operating Manual

HP D300 Digital Dispenser
2
Legal notices
Copyright 2008-2012 Hewlett-Packard Company, L.P.
The HP D300 Digital Dispenser, software and documentation manufactured by the Hewlett-Packard Company, 1000
Circle Boulevard, Corvallis, Oregon 97330. All products made in the U.S.A.
The information contained herein is subject to change without notice.
The only warranties for HP products and services are set forth in the express warranty statements accompanying such
products and services. Nothing herein should be construed as constituting an additional warranty. HP shall not be liable
for technical or editorial errors or omissions contained herein.

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Contents
1Product description
1-1 System requirements .....................................................................................................................................6
1-1-1 Hardware...............................................................................................................................................6
1-1-2 Software ................................................................................................................................................6
1-1-3 Environmental operating conditions ......................................................................................................6
1-1-4 Shipping and storage conditions ...........................................................................................................6
2Safety
2-1 General safety rules.......................................................................................................................................7
2-2 Personal protective equipment.......................................................................................................................7
2-3 Possible inhalation hazard.............................................................................................................................7
2-4 Pinch hazard ..................................................................................................................................................7
2-5 Laser safety....................................................................................................................................................8
2-6 Electrical safety..............................................................................................................................................8
2-7 Electrical emissions safety.............................................................................................................................8
2-8 Decontamination prior to servicing.................................................................................................................9
2-9 Safe disposal of Dispensehead Cassettes and pipette tips ...........................................................................9
2-10 Ergonomic safety .........................................................................................................................................9
2-11 Additional assistance ...................................................................................................................................9
3Clean the dispenser
4Get started
4-1 Start up the dispenser..................................................................................................................................11
4-2 Get familiar with the software.......................................................................................................................12
4-3 Load an existing plate configuration.............................................................................................................14
4-4 Get to know the Options dialog box.............................................................................................................16
4-4-1 Options: General .................................................................................................................................17
4-4-2 Options: Edit: Units..............................................................................................................................17
4-4-3 Options: Edit: Fluid Defaults................................................................................................................18
4-4-4 Options: Edit: Assay Defaults..............................................................................................................19
4-4-5 Options: Plate Jitter.............................................................................................................................19
4-4-6 Options: Report ...................................................................................................................................20
4-5 Plate dispense procedure ............................................................................................................................21
4-5-1 Initiate plate dispense..........................................................................................................................21
4-5-2 Scan and load the well plates..............................................................................................................22
4-5-3 Load a Dispensehead Cassette ..........................................................................................................22
4-5-4 Load fluid into the Dispensehead........................................................................................................23
4-6 View the report.............................................................................................................................................27
4-7 Turn off the HP D300 Digital Dispenser.......................................................................................................30
5Create plate configurations
5-1 Create the fluids table..................................................................................................................................31
5-2 Edit the Fluids List........................................................................................................................................32
5-3 Edit the well plate.........................................................................................................................................33
5-4 Set concentrations or volumes in wells........................................................................................................35
5-5 Use the Paste Special dialog box ................................................................................................................38
5-6 Normalize.....................................................................................................................................................39
5-7 Randomize...................................................................................................................................................41
5-8 Save the plate configuration.........................................................................................................................42
5-9 Plate Layout Wizard basics..........................................................................................................................43
5-9-1 Create single zone titration1layouts ....................................................................................................43
5-9-2 Position NoDispense controls..............................................................................................................47
5-10 Plate Layout Wizard advanced functions...................................................................................................49

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5-10-1 Create multizone titration1 layouts ....................................................................................................49
5-10-2 Create modified range titration1 layouts............................................................................................52
5-10-3 Create titration2 layouts: two fluids per well......................................................................................55
6Troubleshooting and error messages
6-1 Troubleshooting ...........................................................................................................................................58
6-1-1 Problems with software .......................................................................................................................58
6-1-2 Computer issues..................................................................................................................................58
6-1-3 Unable to home stage .........................................................................................................................59
6-1-4 Check the A1 location..........................................................................................................................59
6-1-5 A1 alignment procedure ......................................................................................................................59
6-1-6 Check the fuses and replace if necessary...........................................................................................61
6-2 Error messages............................................................................................................................................64
Appendix A
HP D300 Digital Dispenser software basics.......................................................................................................70
Appendix B
Glossary.............................................................................................................................................................71
Appendix C
Quick Start Guide...............................................................................................................................................72
Appendix D
Revision History .................................................................................................................................................74

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1 Product description
The HP D300 Digital Dispenser dispenses precise amounts of dilute small molecule compounds in
DMSO into standard 96- and 384-well plates. The dispenser is intended to be used in the lab
environment for drug discovery purposes with small molecules in DMSO.
1
Dispensehead Cassette
2
Pogo mechanism
3
Pogo block
4
Source plate holder
5
Motorized stage and destination plate holder
6
Destination plate
7
Deck
8
Power indicator light (HP logo)
9
Dispenser USB connection
10
Power switch
11
PC with D300 software
(Rear view)
(Rear view)
7
9
11
10
4
3
8
1
5
6
2

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1-1 System requirements
1-1-1 Hardware
Dimensions of Dispenser (W x D x H): 47cm x 44cm x 24cm
Grounded electrical outlet (2 required, one for dispenser, 1 for PC):
○120 V (US) or 230 V (Europe) AC power
1 GHz or faster 32 bit (x86) or 64 bit processor
1 GB (32-bit) or 2 GB (64 bit) RAM
16 GB (32-bit) or 20 GB (64-bit) available disk space
Display: 1024 x 768, 256 colors minimum
USB ports
1-1-2 Software
Microsoft Windows 7, Vista or XP
Microsoft .NET Framework 3.5
○.NET 3.5 is included with Windows 7
○.NET 3.5 is available for Windows XP and Vista (www.microsoft.com)
Microsoft Excel 2003 or later
Adobe Acrobat Reader (www.adobe.com/downloads)
Do not adjust text size in Windows –use 100% only
1-1-3 Environmental operating conditions
Temperature: 20C - 30C laboratory conditions (68 - 86F)
Humidity: 30% - 80% RH
1-1-4 Shipping and storage conditions
Temperature: 10C - 32C (50F –90F)
Humidity: 20% - 80% RH

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2 Safety
The HP D300 Digital Dispenser is intended to be used in the lab environment for drug discovery
purposes with small molecules in DMSO only. Other uses of the dispenser are not recommended at
this time because they may introduce safety or performance issues.
Under no circumstances should the dispenser be used to dispense bacteria, viruses, cells, bio-
fluids, biologicals, pathogens, contagions, infectious agents, potent toxins, oncogenes, radioactive
materials or other potentially hazardous substances. The dispenser should not be exposed to Bio
Safety Level 3 (BSL3) or greater conditions, contaminants or locations.
The dispenser should be used in accordance with all applicable safety rules that are in effect in the
lab in which it is installed. Usage of the dispenser should also follow common sense safety
guidelines for chemical, mechanical, and electrical safety.
NOTE: If this unit requires servicing, or you are unsure about any aspect of operation or safety,
contact your Tecan Service Help Desk. The phone number can be found at
http://www.tecan.com/customersupport.
CAUTION: Do not remove any of the dispenser covers. Contact your Tecan Service Help Desk if
the dispenser is not working properly. Do not attempt to repair the dispenser yourself.
2-1 General safety rules
Place the dispenser on a work surface that supports all four feet securely.
Plug the dispenser into a standard, grounded, electrical outlet. Note the power requirement
(120V in North America or 230V in Europe) listed on the back label of the dispenser.
Avoid the moving parts of the dispenser during operation.
2-2 Personal protective equipment
The HP D300 Digital Dispenser dispenses chemicals normally used by your company. The same
safety precautions and personal protective equipment (PPE) specified by your employer for fluid
transfer operations, such as pipetting into bio-assay well plates, should be used when operating the
dispenser and handling these materials.
2-3 Possible inhalation hazard
The dispenser was designed with chemical safety in mind; however, it does dispense picoliter
droplets of fluid and generates a small amount, less than 1% by volume, of very small aerosol
droplets that might not be trapped within the well plate that you are dispensing into. If the fluids are
known or suspected to be an inhalation hazard or contact hazard, consider using the dispenser in
an exhausted enclosure such as a lab fume hood.
2-4 Pinch hazard
The dispenser was designed with mechanical safety in mind; however, there are potential pinch
points between the pogo block and either the Dispensehead Cassette, the Dispenser deck or the
top cover. This pinch hazard is labeled on the pogo block.
CAUTION: Do not place fingers under or on top of the pogo block at any time.

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2-5 Laser safety
A Class 1 laser is used in the dispenser to determine the height of the destination plate. This Class
1 Laser follows the requirements of Laser Notice No. 50 of the FDA (CDRH), Accession No.
0520365-02. This product complies with 21 CFR Part 1040.10 for laser products. See Figure 2-1.
WARNING: Do not look at the laser beam directly. Similarly, do not continue to look at laser light
reflected on a highly reflective substance for a long duration.
Figure 2-1 D300 Digital Dispenser rear placards indicating safety certifications for North America
(top) and Europe (bottom)
2-6 Electrical safety
The HP D300 Digital Dispenser has passed a TUV certification for a CUE marking signifying
compliance with applicable electrical regulations in the EU and North America. As shown in Figure
2-1, a placard is attached to the rear of the dispenser, noting this certification and the electrical
specifications of the dispenser: voltage 120V or 230V AC, 1 phase/3 wire, 50/60 Hertz, 2A full load
amperage, and short circuit interrupt rating 5000A. The dispenser includes two user-replaceable
T2A L250V (time lag, low breaking capacity 2A, 250V) fuses contained in the power inlet module at
the rear of the dispenser.
2-7 Electrical emissions safety
The HP D300 Digital Dispenser has passed an FCC/EMC test for electrical emissions safety. This
device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and (2) this device must accept any
interference received, including interference that may cause undesired operation. Any changes or
modifications to this equipment not expressly approved by the Hewlett-Packard Company may
cause harmful interference, and void your warranty.
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at their own expense. Also, this Class A digital
apparatus complies with Canadian ICES -003.

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2-8 Decontamination prior to servicing
You may be required by Tecan service personnel to certify that appropriate cleaning,
decontamination, and other abatement of potential threats to Tecan service personnel have been
performed prior to shipping the dispenser for servicing. Recommended cleaning and
decontamination procedures are described in the ―Clean the dispenser‖section of this manual.
2-9 Safe disposal of Dispensehead Cassettes
and pipette tips
Disposal of used pipette tips and HP Dispensehead Cassettes (DHCs) should be performed in
accordance with your company’s chemical waste procedures. The DHCs do not require any special
disposal procedures except for those required for the chemicals which have been placed in them by
your laboratory.
2-10 Ergonomic safety
The HP D300 Digital Dispenser typically requires a single manual pipetting operation for each
dispensed compound. In most situations, using the HP dispenser in the intended manner decreases
the frequency of shoulder, arm, and wrist motions and so decreases the stress on these joints.
As with any work task, ergonomics are heavily influenced by workstation setup. To minimize
ergonomic risks associated with the use of the dispenser, consider the following work area design
ideas:
Place the dispenser on a work bench at a height that allows for proper visual and physical
access for operators. Having the dispenser too high can lead to greater shoulder elevation
when pipetting into the Dispensehead and having the dispenser too low can lead to increased
neck and back flexion.
If an operator is too short to comfortably access the dispenser, consider moving the dispenser
to a lower bench height. Alternatively, use a footstool or high lab stool that adjusts to the proper
work height.
Place the dispenser close to the front of the work surface to minimize the distance the operator
must reach to pipette into the Dispensehead.
Place the pipette tip disposal receptacle close to the dispenser.
Place the computer keyboard close to the dispenser to minimize reach distance.
Adjust the computer monitor height and screen angle for good physical and visual access
during dispenser operation.
Use a separate mouse rather than the laptop’s touchpad whenever possible.
Perform data analysis at a separate ergonomically designed workstation whenever possible
rather than at the dispenser computer.
2-11 Additional assistance
Contact your local Tecan Service Help Desk for additional assistance. The phone number can be
found at http://www.tecan.com/customersupport.

/Clean the dispenser
HP D300 Digital Dispenser
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3 Clean the dispenser
WARNING: Observe the following cautions when cleaning the dispenser:
Do not spray cleaning fluid directly onto the Dispenser when a Dispensehead
Cassette is installed. Always avoid exposing unused Dispenseheads to cleaning
fluids.
Do not spray or wipe the ionizer bar that is mounted under the deck between the
plate opening and the Dispenseheads.
Do not spray or wipe the sensors under the Dispenser deck. Doing so could cause
the sensors to become misaligned.
Do not wipe the pogo pins. See Figure 3-2.
Figure 3-2 Pogo pin region of pogo block which should not be sprayed or wiped with cleaning fluid
As with any lab instrument, it is a best practice to routinely clean areas of the Dispenser that come
into contact with chemicals.
1. Turn off the dispenser as instructed in the ―Turn off the HP D300 Digital Dispenser‖ section
of this document.
2. Remove any unused Dispensehead Cassettes from the Dispenser.
CAUTION: Exposing unused Dispensehead Cassettes to cleaning fluids may compromise their
performance.
3. Spray a cleaning solution, such as a bleach or a 70% ethanol solution, onto a fabric Texwipe or
other cloth that does not generate particles.
4. Wipe down all accessible Dispenser surfaces, including the following (See the ―Product
description― section for the location of described areas):
○The top and bottom surfaces of the dispenser deck, avoiding the ionizer bar in the cutout
on the right side of the bottom of the deck and the sensors at the front corners of the deck
○The pogo mechanism housing, excluding the pogo pin area as described above
○The destination plate holder
○The source plate holder
○All of the outside surfaces of the dispenser