Human HuMax 14K User manual

HuMax 14K
|User Manual
Cat No. 17160/1

REVISION LIST OF THE MANUAL
Rev. /DATE. REVISION DESCRIPTION
01/2005-06 First edition
02/2008-11 New corporate design, correction of typing errors
03/2010-05 New RCF function considered
04/2011-09 Correction dimension
05/2011-10 Intended Use correction, Accelaration and Braking rates
changed, required Adapter corrected
06/2016-02 3.1 Technical Specifications Table, power supply update
07/2017-09 New design and new operation unit
08/2020-12 Technical drawing update
SYSTEM VERSION
COPYRIGHT
Copyright 2020, Human Gesellschaft für Biochemica und Diagnostica mbH, Wiesbaden,
Germany. All rights reserved.
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of HUMAN in
writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
SERVICE AND SUPPORT

CONTENTS
TABLE OF CONTENTS
1 SAFETY INSTRUCTIONS 5
1.1 INTRODUCTION 5
1.2 USER WARRANTY 5
1.3 INTENDED USE OF THE INSTRUMENT 6
1.4 GENERAL SAFETY WARNINGS 6
1.5 DISPOSAL MANAGEMENT CONCEPT 7
1.6 INSTRUMENT DISINFECTION 7
1.7 BIOHAZARD WARNING 8
2 MAIN PURPOSE OF THE UNIT AND DESCRIPTION 9
3 TECHNICAL SPECIFICATIONS 11
3.1 TECHNICAL SPECIFICATIONS TABLE 11
3.2 OPERATING PRINCIPLES 11
3.2.1 Drive System 11
3.2.2 Imbalance Detection System 12
3.3 ROTOR SELECTION TABLE 13
4 INSTALLATION PROCEDURE 15
4.1 LIFTING AND TRANSPORT 15
4.2 CONTENTS OF PACKAGE 15
4.3 MAINS SUPPLY 15
4.4 ENVIRONMENTAL CONDITIONS 15
4.5 POSITIONING 16
4.6 MANUAL LID OPENING 16
4.7 PREPARATION OF THE ROTOR TO RUN 16
4.7.1 Rotor Installation 17
4.7.2 Loading 17
5 CONTROL PANEL AND INDICATORS 19
5.1 CONTROL PANEL, DISPLAY 19
5.2 PREPARING TO RUN 21
5.3 MAKING A NEW PROGRAM 21
5.3.1 Selecting the memorized program 22
5.3.2 Pulse mode 23

5.4 END OF OPERATION 23
5.5 SAFETY INTERLOCK SYSTEM 24
6 CLEANING & PERIODICAL MAINTENANCE 25
6.1 PERIODICAL MAINTENANCE 25
6.2 DISINFECTION / STERILISATION 25
6.3 CORROSION INFORMATION 26
6.3.1 Chemical Corrosion 26
6.3.2 Stress Corrosion 26
6.4 CLEANING 27
6.5 ELECTRICITY 27
7 TROUBLESHOOTING 29
8 ELECTRICAL CIRCUIT DIAGRAMS 31
8.1 ELECTRICAL CIRCUIT DIAGRAM OF HUMAX 14K 31
8.2 ELECTRICAL CIRCUIT DIAGRAM OF HUMAX 14K R 32

CONTENTS

Safety InStructIonS 5
1 SAFETY INSTRUCTIONS
1.1 Introduction
This manual is considered as a part of the instrument; it has to be at the oper-
ator’s hand as well as at the maintenance operator’s availability. For accurate
installation, use and maintenance, please read the following instructions care-
fully. In order to avoid instrument damage or personal injury, carefully read the
”GENERAL SAFETY WARNINGS”, describing the suitable operating procedures. In
case of breakdowns or any troubles with the instrument, apply to the local Tech-
nical Service.
1.2 User warranty
HUMAN warrants that instruments sold by one of its authorised representa-
tives shall be free of any defect in material or workmanship, provided that this
warranty shall apply only to defects which become apparent within one year
from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item at no
charge, except for transportation expenses to the point of repair.
This warranty excludes the HUMAN representative from liability to replace
any item considered as expendable in the course of normal usage, e.g.: lamps,
valves, syringes, glassware, fuses, diskettes, tubing etc.
The HUMAN representative shall be relieved of any liability under this warranty
if the product is not used in accordance with the manufacturer‘s instructions,
altered in any way not specified by HUMAN, not regularly maintained, used
with equipment not approved by HUMAN or used for purposes for which it was
not designed.
HUMAN shall be relieved of any obligation under this warranty, unless a com-
pleted installation / warranty registration form is received by HUMAN within
15 days of installation of this product.
This warranty does not apply to damages incurred in shipment of goods. Any
damage so incurred shall be reported to the freight carrier for settlement or
claim.

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1.3 Intended use of the instrument
The instrument is intended for laboratory application by professional us-
ers. It has to be used for the expected purposes and in perfect technical con-
ditions, by qualified personnel, in working conditions and maintenance
operations as described in this manual, according to the GENERAL SAFETY
WARNINGS. This manual contains instructions for professional qualified
operators.
1.4 General safety warnings
Use only chemical reagents and accessories specified and supplied by
HUMAN and/or mentioned in this manual. Place the product so that it has proper
ventilation.
The instrument should be installed on a stationary flat working surface, free
from vibrations.
Do not operate in area with excessive dust.
Work at room temperature and humidity, according to the specifications listed
in this manual.
Do not operate this instrument with covers and panels removed.
Only use the power cord specified for this product, with the grounding conduc-
tor of the power cord connected to earth ground.
Use only the fuse type and rating specified by the manufacturer for this instru-
ment, use of fuses with improper ratings may pose electrical and fire hazards.
To avoid fire or shock hazard, observe all ratings and markings on the
instrument.
Do not power the instrument in potentially explosive environment or at risk of
fire.
Prior to cleaning and/or maintaining the instrument, switch off the instrument
and remove the power cord.
For cleaning use only materials specified in this manual, otherwise parts may
become damaged. It is recommended always to wear protective apparel and
eye protection while using this instrument. Respective warning symbols, if
appearing in this manual, should be carefully considered.

Safety InStructIonS 7
1.5 Disposal management concept
The currently valid local regulations governing disposal must be observed. It is in
the responsibility of the user to arrange proper disposal of the individual
components.
All parts which may comprise potentially infectious materials have to be
disinfected by suitable validated procedures (autoclaving, chemical treatment)
prior to disposal. Applicable local regulations for disposal have to be carefully
observed.
The instruments and electronic accessories (without batteries, power packs etc.)
must be disposed off according to the regulations for the disposal of electronic
components.
Batteries, power packs and similar power source have to be dismounted from
electric/electronic parts and disposed off in accordance with applicable local
regulations.
1.6 Instrument disinfection
Analytical instruments for in vitro diagnostic involve the handling of human
samples and controls which should be considered at least potentially infec-
tious. Therefore every part and accessory of the respective instrument which
may have come into contact with such samples must equally be considered as
potentially infectious.
Before doing any servicing on the instrument it is very important to thorough-
ly disinfect all possibly contaminated parts. Before the instrument is removed
from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination should be performed by authorised well-trained personnel
only, observing all necessary safety precautions. Instruments to be returned
have to be accompanied by a decontamination certificate completed by the
responsible laboratory manager. If a decontamination certificate is not sup-
plied, the returning laboratory will be responsible for charges resulting from
non-acceptance of the instrument by the servicing centre, or from authority’s
interventions.

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1.7 Biohazard warning
Analytical instruments for in vitro diagnostic application involve the handling of
human samples and controls which should be considered at least potentially in-
fectious. Therefore every part and accessory of the respective instrument which
may have come into contact with such samples must equally be considered as
potentially infectious.
For safety reasons, we have labeled instruments with the „BIOHAZARD“ warn-
ing label below.
FIGURE 1
Biological Hazard Symbol

MaIn PurPoSe of tHe unIt anD DeScrIPtIon 9
2 MAIN PURPOSE OF THE UNIT AND DESCRIPTION
The HuMax 14K centrifuges are designed to separate liquids of different
densities by applying centrifugal force.
Different types of rotors, swing-out and angle rotors, can be fitted to the
centrifuges.
The microprocessor control system also displays the RCF value during the run.
The ventilation system of the HuMax 14K limits temperature in the bowl at
max. +12°C.
The centrifuges are manufactured according to the following standards:
EN 61010-1, EN 50081-1, EN 980

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tecHnIcaL SPecIfIcatIonS 11
3 TECHNICAL SPECIFICATIONS
3.1 Technical Specifications Table
HuMax 14K
Max Speed 17161 rotor: 4100 rpm, 17163 rotor: 14000 rpm
Max. RCF* 17161 rotor: 3045 x g, 17163 rotor: 18407 x g
Max. Capacity 17161 rotor: 4x 200 ml, 17163 rotor: 24 x 1.5/2 ml
Control System Microprocessor controlled
Display Digital, High Visibility
No. of Memories 10
Speed Range 500-14000 rpm
Speed Step 10 rpm
Speed Accuracy ± 20 rpm
Timer Range 01-99 min + Hold Position
Timer Step 1 min
Acceleration Rate 10 acceleration rates, 0: slowest, 9: fastest
Braking Rate 10 braking rates, 0: slowest, 9: fastest
Power Supply 230 V ± 10%, 50/60 Hz
Max. Power 500 W
Average Power 300 W
Dimension WxDxH Instrument without any components :
465 x 550 x 400 mm
Space required for routine use:
565 x 650 x 870 mm
Packaging:
520 x 600 x 490 mm
Net Weight 43.4 kg
Gross Weight 47.6 kg
* RCF: Relative Centrifugal Force
3.2 Operating Principles
3.2.1 Drive System
The rotor is driven by a three phase asynchronous motor. The microprocessor
control system assures the correct drive speed.
The force applied to the rotor is directly related to the shape of the rotor, the
swing-out rotor receives more force than the angle rotor does.
TABLE 1

Gerätename | User manual
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Longer radius and more accessories increase the load of the rotor and decrease
the spinning speed.
The centrifuge does not allow the rotor to spin at a speed that it cannot resist
mechanically.
Please make that sure the correct type of rotor is selected during programming.
SAFETY INTERLOCK SYSTEM
The safety interlock system prevents opening of the lid while the rotor is
spinning.
The centrifuge does not operate until the lid is closed and the lid remains locked
until the rotor stops spinning. The “Lid Open” indicator turns on to warn the
user if the lid is not closed properly.
3.2.2 Imbalance Detection System
The imbalance detection system of the HuMax 14K centrifuges operates
electronically when an unacceptable imbalance occurs. In this case the brake is
applied immediately.
The “Err 1” imbalance error is displayed and the imbalance indicator turns on.
The lid cannot be opened until the rotor stops spinning. The centrifuge can only
be started after the lid has been opened and the rotor has been re-loaded cor-
rectly.
To avoid facing imbalance problems, please make sure to balance the load.
If power failure occurs, access to the samples is
possible by opening the lid
with a special tool. Please
see the manual lid opening
section (4.6) for further in-
formation.

tecHnIcaL SPecIfIcatIonS 13
3.3 Rotor Selection Table
Rotor Description Capacity Max. Tube
Dim. mm
Rad mm Max. Speed
Rpm
Max RCF xg
17161 Swing-Out Rotor 4x 200 ml Ø 57 162 4100 3045
17161/1 Set of 4 buckets 200 ml Ø 57 162 4100 3045
17161/3 4 PP bottles with cap 200 ml Ø 56.5 162 4100 3045
17161/4 Set of 4 inserts 1x100 ml Ø 45 162 4100 3045
17161/5 Set of 4 inserts 1x50 ml Conical Ø 30 162 4100 3045
17161/6 Set of 4 inserts 7x15 ml Ø 17 162 4100 3045
17161/7 Set of 4 inserts 3x15 ml Conical Ø 17 162 4100 3045
17161/8 Set of 4 inserts 7x5/7ml Ø 13.5 162 4100 3007
17162 Angle rotor 30x15 ml Ø 17 160 4100 3007
17157 Adapter 1 x 5 ml Ø 13 160 4100 3007
17156 Adapter 1 x 7 ml Ø 13 160 4100 3007
17155 Adapter 1 x 1.5/2 ml Ø 11 160 4100 3007
17163 Angle Rotor 24x 1.5/2 ml Ø 11 84 14000 18405
17161/1*Adapter 1x500/800 µl Ø 8 84 14000 18405
17161/2*Adapter 1x 200 µl PCR Ø 6.5 84 14000 18405
17161/3*Adapter 1x250/400/700µl Ø 6.5 84 14000 18405
* For 17163 is required to use 24 adapters.
Insert set is not needed for 200 ml tubes.
TABLE 2
FIGURE 2

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19
8
76
5
4
3
2
10
11
12
FIGURE 3
1 Lid
2 Lock Pin
3 Gas Spring
4 Lid Gasket
5 Rotor
6 Manual LID Opening Hole
7 Control Panel
8 Bowl
9 Observation Window
10 On/Off Switch
11 Electrical Socket And Fuses
12 Air Ventilation

InStaLLatIon ProceDure 15
4 INSTALLATION PROCEDURE
4.1 Lifting and Transport
Because of the heavy weight of the centrifuge, all lifting and transport must be
carried out by using proper handling equipment. The centrifuge should be lifted
from underneath and never be turned over.
4.2 Contents Of Package
The package includes:
-1 ea. HuMax 14K Centrifuge
-1 ea. User manual
-1 ea. Manual lid opening tool
-1 ea. Electrical cable
-1 ea. 24-Socket wrench (nut & lever)
-1 ea. Bucket lubrication grease
-1 ea. Puller tool
4.3 Mains Supply
Max. power requirement: 500 VA (average: 300 VA)
Max. power requirement: 800 VA (average: 500 VA)
The centrifuges should be connected to grounded plugs.
4.4 Environmental Conditions
The centrifuge is designed to operate safely under the following conditions:
-Indoor use only
-Ambient temperature: 5°C to 40°C
-Maximum relative humidity of 80% for temperature up to 22°C
-Maximum altitude: 2000 m
Maximum performance is obtained between 15°C and 25°C.

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4.5 Positioning
-Make sure that no damage has occurred during transport.
-Make sure that the positioning is suitable for users.
-Lift the centrifuge underneath and carry it to its place.
-The bench-top must be rigid enough to support the weight and vibrations.
-Leave 30 cm free space on all sides of the centrifuge.
-Open the lid using the unlocking tool and make sure that no objects are left
in the bowl. (See the manual lid opening section 4.6).
-Make sure that the centrifuge does not occupy the utilisation space of near-
by equipment or do damage to it.
-The user should check the centrifugation process during the operation.
-Make sure that the mains supply is suitable for the instruments’ power
consumption (230 V, 50 Hz, grounded plug).
4.6 Manual Lid Opening
In case of power cut or any breakdown, the centrifuge can be opened manually
to access the samples.
To open the lid manually:
-Switch off the instrument.
-Insert the manual lid opening tool into the hole on the left side of the
instrument (see the general view drawing).
-Push the tool downward, keeping it vertical until the lid opens.
4.7 Preparation of the Rotor to run
Before installation, check the rotor for corrosion and cleanliness.
Chemical or mechanical corrosion may cause severe damage to the rotor and
the centrifuge. Particles which are stuck inside the inserts cause the breakage of
tubes and lead to major imbalance please check to make sure that no particles
are left on the rotor.
The central hole of the rotor and the motor shaft should always be kept clean
and dry, especially before any centrifugal operations.
This operation must be
carried out by someone
who is informed of the dan-
ger and of the precautions
that must be taken. Before
opening the lid manually,
make sure that the rotor has
stopped spinning. Upon
opening the lid, lift it by
hand and observe the rotor.
If the rotor is still spinning,
close the lid and wait ap-
proximately 10 minutes be-
fore repeating the opera-
tion.