Human Elisys Quattro User manual

HUMAN -
ELISYS QUATTRO
User Manual
Elisys Quattro
| User Manual
|
Cat.No. 16300/1

Edition:
System Manufacturer:
HUMAN Gesellschaft für Biochemica und Diagnostica mbH
Software-copy-
rights
The ELISYS QUATTRO software is the intellectual property of HUMAN GmbH .
Intellectual property rights shall remain with
HUMAN GmbH
You are entitled to use the ELISYS QUATTRO software and the printed accompany-
ing material at your place of work only.
Any violations of property rights or copyright or trademark may be subject to legal
action.
Rev. /DATE. REVISION DESCRIPTION
01/2004-12 First edition
02/2011-01 Update cover and back page
Copyright © 2010, HUMAN GmbH . All rights reserved.
HUMAN Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21
D-65205 Wiesbaden, GERMANY
Neither this manual nor any parts of it may be duplicated or transmitted in any way without the written
approval of
HUMAN GmbH .

ELISYS QUATTRO - User Manual HUMAN TOC-1
Table of Contents
1 Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.1 Typographical Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.1.1 Warning Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.1.2 Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.1.3 Symbol Keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.1.4 Special Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.2 Safety Instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.2.1 General Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1.2.2 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
1.2.3 Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1.2.4 Mechanical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1.2.5 Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
1.2.6 Cleaning the System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
1.2.7 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
1.3 Positions of Safety Labels and Type Label. . . . . . . . . . . . . . . . . 1-7
1.3.1 General Warning Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
1.3.2 Biological Hazard Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
1.3.3 Electrical Hazard Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
1.3.4 Laser Hazard Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
1.3.5 Type Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
2 System Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.1 Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 Top Level Instrument Modules. . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2.2.1 Tray for Tip Racks and Pre-dilution . . . . . . . . . . . . . . . . . . . . . . 2-3
2.2.2 Patient Sample and Reagents Unit with Bar Code Reader . . . . 2-4
2.2.3 Tip Ejection Station, Pipettor Wash Station, Pipetting Station. . . 2-4
2.2.4 Test Plate Compartment with Bar Code Scanner . . . . . . . . . . . . 2-5
2.3 Drawer Instrument Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.3.1 Wash Buffer Container and Waste Bottles . . . . . . . . . . . . . . . . . 2-6
2.3.2 Wash Unit for Test Plates with Window . . . . . . . . . . . . . . . . . . . 2-6
2.3.3 Incubator Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2.3.4 Photometer (Back of Bottom Drawer). . . . . . . . . . . . . . . . . . . . . 2-7
2.4 Other Instrument Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
2.4.1 Guide Rail for Pipettor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
2.4.2 Pipettor (Z-Direction) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2.5 Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2.6 LED Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
2.7 Principles of Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
2.7.1 Absorbance Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
2.7.2 Bichromatic Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
3 Software Structure and Operation . . . . . . . . . . . . . . . . . . . 3-1
3.1 Basic Information about Operation . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Menu Guidance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.1 General Menu Guidance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.2 Worklist Menu Guidance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3.2.3 Result Menu Guidance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3.3 File Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
4 Performing a Test Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

TOC-2 ELISYS QUATTRO - User Manual HUMAN
4.1 System Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.2 Input of Patient Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.2.1 Manual Input of Patient Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.2.2 Input of Bar Coded Patient Samples . . . . . . . . . . . . . . . . . . . . . . 4-4
4.3 Worklist Definition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.3.1 Create New Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.3.2 Defining Several Assays per Plate. . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.3.3 Defining Several Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.3.4 Input of Reagent Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.4 Start a Worklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4.4.1 The Worklist Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4.4.2 Loading Samples and Reagents . . . . . . . . . . . . . . . . . . . . . . . . 4-12
4.4.3 Loading other Required Resources . . . . . . . . . . . . . . . . . . . . . . 4-15
4.4.4 Reagent Volume Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
4.4.5 Plate Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-17
4.4.6 Starting and Processing a Run . . . . . . . . . . . . . . . . . . . . . . . . . 4-18
4.4.7 Pausing or Cancelling a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-20
4.5 Results of a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-20
4.5.1 Results Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-21
4.6 Re-Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-23
4.7 Quality Control Analysis Report (Levey Jennings Plot) . . . . . . 4-25
4.8 Patient Result Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-25
5 Loading - Unloading - Disposal . . . . . . . . . . . . . . . . . . . . . 5-1
5.1 Loading (Instrument at Hold). . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.1 Refill System Liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.2 Refill Wash Liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.3 Position Dilution Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.4 Load Tip Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.1.5 Load Sample and Reagent Racks . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5.1.6 Load Test Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.2 Unloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
5.2.1 Remove Dilution Tubes / Take Tip Racks off . . . . . . . . . . . . . . . . 5-6
5.2.2 Remove Racks with Patient Samples and Reagents. . . . . . . . . . 5-6
5.2.3 Remove Test Plate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
5.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
5.3.1 Liquid Waste from Pipettor and Wash Unit . . . . . . . . . . . . . . . . . 5-7
5.3.2 Disposable Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
5.3.3 Replace Waste Container for Disposable Tips . . . . . . . . . . . . . . 5-7
5.3.4 Disposal of Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
5.3.5 Disposal of Packaging Material . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
6 Service and Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.1 Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.1.1 Daily Maintenance Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.1.2 Weekly Maintenance Procedure . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
6.1.3 Monthly Maintenance Procedure . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
6.2 Service Plan (to be performed by authorised Service Engineers)6-
2
6.3 Cleaning and Disinfecting the Instrument . . . . . . . . . . . . . . . . . . 6-4
6.3.1 Cleaning and Disinfecting the Housing Surface. . . . . . . . . . . . . . 6-5
6.3.2 Cleaning and Disinfecting the Interior of the Instrument . . . . . . . 6-5
6.3.3 Maintenance of Pipettor System . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
6.3.4 Maintenance of Washer System . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
6.4 Cleaning the Wash Head Needles . . . . . . . . . . . . . . . . . . . . . . . . . 6-6
6.5 Replacement of Mains Fuse. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-7
6.6 Backup of Result Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-8
6.7 Putting System Out of Action . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-8

ELISYS QUATTRO - User Manual HUMAN TOC-3
7 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.1 General Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
7.2 Error Messages of Components. . . . . . . . . . . . . . . . . . . . . . . . . . 7-6
8 Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1

TOC-4 ELISYS QUATTRO - User Manual HUMAN

Intended Use
ELISYS QUATTRO - User Manual HUMAN 1-1
1 Intended Use
The ELISYS QUATTRO system consists of the ELISYS QUATTRO instrument and
the Phoenix software. It is a fully automated microplate analyzer performing the
complete sample processing (sample dilutions, sample and reagent dispensing, in-
cubations, wash processes, plate transports) as well as the photometric measure-
ment and evaluation.
For in Vitro Diagnostics only!
Prior the use of the ELISYS QUATTRO system for IVDs, any test methods (assays)
and kits must be validated by the user in combination with the system according to
common clinical laboratory practice, local legislation, and the state of the art.
1.1 Typographical Conventions
Please consider the following typographical conventions.
1.1.1 Warning Messages
Warning messages are indicated with a safety symbol and printed in special types.
For special situations the following security symbols were used.
Caution, risk of danger! Consult instructions for use!
Biohazard!
Electrical hazard!
Caution, hot surface!
Mechanical hazard!
Automatic start-up!
Laser hazard!

1-2 ELISYS QUATTRO - User Manual HUMAN
Intended Use
1.1.2 Notes
Notes are indicated with a symbol and printed in special types. For special situations
were used the following symbols.
Consult instructions for use!
Notes are indicated by this symbol and printed in special types.
Disconnect mains power connector before servicing!
1.1.3 Symbol Keys
1.1.4 Special Types
Manufactured by
In Vitro Diagnostic Medical Device
Lot Number
Expiration Date
Storage Temperature
CE Mark
Catalogue Number
Serial Number
LEDs LEDs (light emitting diode) are printed in spe-
cial type.
Example:
PWR LED, ERR LED

Intended Use
ELISYS QUATTRO - User Manual HUMAN 1-3
1.2 Safety Instructions
Read all of these instructions! Save these instructions for later use!
Please always act according to the following safety instructions, before as
well as during operation of the system!
The operating manual must be kept near the system and must be accessible
for the user at any time.
ELISYS QUATTRO is a fully automated microplate analyzer including functions such
as sample preparation, test performance, photometric measurement and data eval-
uation.
ELISYS QUATTRO is designed and manufactured in accordance with the safety re-
quirements for electronically and IVD measuring devices. If the local law lays down
regulations on the installation and/or operation of microplate analyzers, then it is the
operators responsibility to adhere to them.
The manufacturer has done everything possible to guarantee that the equipment
works safely, both electrically and mechanically. The systems are tested by the man-
ufacturer and supplied in a condition that allows safe and reliable operation.
The user has to observe the information and warnings contained in this operating
manual in order to ensure safe operation of the instrument.
1.2.1 General Safety
Follow all warnings and instructions marked on the instrument and in this
manual.
The instrument must only be operated by personnel who have been trained on
the use of the system.
It is strongly recommended that all first time personnel read this manual prior to use.
Use the system only for the designated application.
Use only the consumables and accessory prescribed herein (e. g. microplates,
tubes, disposable tips etc.).
The manufacturer assumes no liability for any damages, including those to third par-
ties, caused by improper use or handling of the system.
Menu items and fields Menu items and fields are printed in bold type.
Example:
File Menu
Buttons Buttons are printed in spaced type.
Example:
Open button.
Keys Keys are printed in slanted type.
Example: Press Enter

1-4 ELISYS QUATTRO - User Manual HUMAN
Intended Use
The operator may only perform the maintenance work described in this manu-
al.
Use only the parts prescribed in this manual for servicing.
The tests and maintenance work defined by the manufacturer should be performed
to make sure that the operator remains safe and that the instrument continues to
function correctly.
Any service and maintenance work not described in this manual must be performed
by authorised service personnel and technicians.
Any changes on the system which are not authorised by the manufacturer lead to the
loss of guarantee obligation.
The system was developed and tested according to the regulations of the IvD
directive
Any changes on the system which are not authorised by the manufacturer lead to the
loss of the validity of the conformity with the applicable regulations the manufacturer
has declared. In this case, the customer is responsible for the fulfilment of the appli-
cable regulations.
The instrument should be opened, serviced and repaired by qualified and au-
thorised service personnel only.
1.2.2 Electrical Safety
Country-specific regulations regarding safe electrical operation of the in-
stalled system have to be observed.
Check that the operating voltage is set correctly on all system components be-
fore you connect the system to the mains supply.
This product must be operated from the type of power source indicated on the type
label. If you are not sure of the type of power available, consult an authorised sales
person or your local electric power company.
Use a 3-wire grounding type plug to connect all instrument and peripherals to
mains supply.
Use only extension cables with a protective conductor and grounded contact.
Grounding of the instrument, computer and its peripheral devices to the same pro-
tective earth potential must be ensured.
Never knowingly interrupt the grounding contacts.
There is the risk of an electrical shock if the protective conductor is interrupted within
or outside the device, or has been disconnected.
The instrument must be connected with a delivered connection cable to dedi-
cated socket. The use of a multi plug is not allowed!
Do not allow anything to rest on the power cord.
If you can see that the instrument has become unsafe to use, switch it off and
disconnect it from the mains supply.

Intended Use
ELISYS QUATTRO - User Manual HUMAN 1-5
If liquid gets inside the instrument, switch it off and disconnect it from the
mains supply. Clean, and dry the respective parts.
Surfaces (floors, work table) must be dry when you are working with the sys-
tem.
Only use the bottles, tubing and components supplied and recommended for con-
taining liquids on the instrument.
Spare fuses must match the values (nominal voltage, nominal current, and
type) specified by the manufacturer.
Always replace blown fuses, don't try to repair them.
Never short-circuit the fuse holder.
Switch the instrument off and disconnect it from the mains supply before serv-
icing.
Only when directed to do so, should power be applied. If power is applied while any
covers are removed, use extreme caution while servicing the system.
Never remove protective guards or secured components since you could ex-
pose live parts in this way.
Electrical connection contacts (plugs, sockets etc.) can be electrically live.
Even after the device has been switched off, components (e. g. capacitors) can be
under voltage as the result of an electrical charge.
All current-carrying parts are sources of danger for an electrical shock.
Don't position the instrument so that it is difficult to operate the disconnecting
device (power supply switch, mains power connector)!
1.2.3 Laser Safety
Care must be taken when operating and testing the bar code scanners as they
use a laser class 2. Never look directly into the laser beam!
Output causes iritations of the eye if stared into the beam for long periods of time.
See for technical data of the laser (bar code scanner).
For operating and testing the laser, no optical devices may be used.
For operating and testing the laser, watches and mirroring jewellery should be re-
moved.
Use of controls or adjustments or performance of procedures other than those
specified herein may result in hazardous radiation exposure.
1.2.4 Mechanical Safety
Do not place the instrument on a unstable table, cart, or similar.
The instrument may fall, causing serious damage to the system or injure the user.

1-6 ELISYS QUATTRO - User Manual HUMAN
Intended Use
Don't load the complete opened drawer! The instrument could tip forward!
Never open screw-attached housing parts while the instrument is on.
There is a risk of injury due to moving parts (fan, motor drives).
Only when directed to do so, should power be applied. If power is applied while any
covers are removed, use extreme caution while servicing the system.
Do not take off the protective cover during a run and do not reach into the
working area.
Improper handling may cause serious damage to the instrument or injure the user.
If you open the flap or cover, verify that the movement of the pipettorhas stopped be-
fore you reach in the working area.
Avoid touching the pipettorand other moving parts while the system is in operation.
Notice that it is possible that the instrument continues processes independently, even
without computer.
Slots and openings are provided for ventilation.
To ensure reliable operation of the instrument and to protect from overheating, these
openings must not be blocked or covered.
1.2.5 Biological Safety
Risk of infection! Handling of samples and reagents:
Avoid contact between skin/mucous membrane and samples/test reagents or parts
of the instrument which were in contact with samples/test reagents.
The above-mentioned parts are to be treated as being potentially infectious.
Reagents can lead to irritation of the skin and mucous membranes.
Use appropriate gloves!
Observe the instructions in the package inserts for a correct use of the reagents.
If sample material is spilled in the system, clean and disinfect it immediately
by the use of a validated method.
For reagent containers and tubings (system liquid and waste), no guarantee
can be provided for any resistance against organic solvents.
For this reason, do not use any organic solvents unless such solvents are expressly
authorised.
It is not possible to autoclave the containers for liquids and waste!
1.2.6 Cleaning the System
Switch the instrument off and disconnect it from the mains supply before
cleaning, disinfection or decontamination.

Intended Use
ELISYS QUATTRO - User Manual HUMAN 1-7
Liquid detergents, disinfectants or decontamination liquids may not be poured
into the instrument or sprayed inside the system.
For the cleaning, disinfection or decontamination, a cloth moistened with detergent,
disinfectant or decontamination liquid should be used.
Only approved detergents, disinfectants or decontamination liquids may be used.
Only approved cleaning, disinfection or decontamination methods may be used.
For cleaning, disinfection or decontamination, the regulations of the local authorities
must be observed.
1.2.7 Disposal
Potential infectious material and all parts that may come in contact with poten-
tial infectious material must be disposed according to national legislation.
All parts which have been replaced, must be disposed according to national
legislation.
Disposal of the instrument according to national legislation.
Disposal of the packaging material according to national legislation.
Containers intended for single-strip use may not be used repeatedly.
1.3 Positions of Safety Labels and Type
Label
If any label gets lost, replace it by an equivalent label!
1.3.1 General Warning Labels
General waring labels are positioned on:
• Patient sample and rack unit flap
1.3.2 Biological Hazard Labels
Biological hazard labels are positioned on:
• Waste bag cover
• Waste container
• Waste bottles (positioned in the drawer)

1-8 ELISYS QUATTRO - User Manual HUMAN
Intended Use
1.3.3 Electrical Hazard Labels
Electrical hazard labels are positioned on:
• Rear side of the instrument (near the mains switch)
1.3.4 Laser Hazard Labels
Laser hazard labels are positioned on:
• Patient sample and reagent unit bar code scanner on the right side at the
front).
• Test plate compartment (near the bar code scanner on the left side at the
front).
1.3.5 Type Label
The type label in positioned on:
• Rear side of the instrument (near the mains switch)

System Basics
ELISYS QUATTRO - User Manual HUMAN 2-1
2 System Basics
2.1 Overview
The ELISYS QUATTRO is a fully automated microtiter plate analyzer performing the
complete sample processing (sample pre-dilutions, sample and reagent dispensing,
incubations, wash processes, plate transports) as well as the photometric measure-
ment and evaluation. The instrument is controlled via the Windows PC software
Phoenix. This software, which was specifically designed for this purpose, allows the
user to process the pre-defined assays as well as assays programmed by the user.
The clear structure with intuitive user-guidance allows simple and quick operation of
daily routine jobs as well as programming of user-specific assays.
Figure 2-1: ELISYS QUATTRO - Instrument modules
1 Tray for tip racks and dilution tubes
2 Patient sample and reagent rack unit with bar code scanner
3 Tip eject station, pipettor wash station, pipetting station
4 Test plate compartment, plate transport unit
5 Drawer with wash unit and photometer
6 Position of incubators (below test plates), heated and for room
temperature (dark)
7 Waste bag for tips
8 Guide rail for pipettor (X and Y movement)
9 Pipettor (movement in Z-direction)

2-2 ELISYS QUATTRO - User Manual HUMAN
System Basics
2.2 Top Level Instrument Modules
Figure 2-2: ELISYS QUATTRO - View on top level
1a Dilution plates (see chapter 2.2.1 on page 2-3)selftest
1b Tip racks (see chapter 2.2.1 on page 2-3)
2 Patient sample and reagent unit (see chapter 2.2.2 on page 2-4)
3a Tip ejection station and waste bag (see chapter 2.2.3 on page 2-4)
3b Pipettor wash station (see chapter 2.2.3 on page 2-4)
3c Pipetting station (see chapter 2.2.3 on page 2-4)
4 Test plate compartment with plate transport unit (see chapter 2.2.4 on
page 2-5)

System Basics
ELISYS QUATTRO - User Manual HUMAN 2-3
2.2.1 Tray for Tip Racks and Pre-dilution
The tray is accessible via a drawer which is locked automatically as soon as a run
has been started. The tray includes 5 positions for tip racks (300 l or 1100 l tips,
see figure 2-2: position 1b) as well as a position for the dilution rack see figure 2-2: position
1a. 6 additional reagent positions are also available.
Move the tip rack from above straight into the specific position, the groove must fit
into the pin (see figure 2-3: and see chapter 5.1 on page 5-1). The clamp shall fix the tip rack.
Figure 2-3: Placement of tip racks: A1 is on the top left

2-4 ELISYS QUATTRO - User Manual HUMAN
System Basics
2.2.2 Patient Sample and Reagents Unit with Bar
Code Reader
(See figure 2-2: position 2)
CAUTION: Laser radiation - do not stare into beam!
The bar code scanner is located on the right side at the front of the patient sample
and reagent unit.
The patient sample and reagent unit, accessible via a flap, includes 12 tracks for
sample and reagent racks.
The following racks are supplied:
Each rack includes a contact pin; on racks occupying one track, this pin is located at
the top centre, and on the broader racks at the top right.
The software specifies which track is to be used for the respective rack. This is indi-
cated by a red LED. A reagent rack occupying 3 tracks must be inserted such that
the contact tappet is in contact with the lit up LED.
Each rack has to be inserted up to the limit stop. The respective LED on the rear pan-
el goes off (Loading/Unloading see chapter 5 on page 5-1).
Reloading of patient and reagent racks is possible when the instrument is in the in-
cubation mode.
Keep the flap of this unit closed during a run. It may be opened only for re-
loading (see chapter 5.2 on page 5-6). Opening the flap while transfer steps are
being performed results in an immediate stop of the pipettor and entry in the
event log.
2.2.3 Tip Ejection Station, Pipettor Wash Station,
Pipetting Station
Tip ejection station and waste bag (see figure 2-2: position 3a)
The opening serves as ejection station for disposable tips. The ejected tip is trans-
ported into the waste bag via a slide which is attached to the front side of the instru-
ment. The ejection station is closed by a cover plate which can be pulled off by hand.
With the cover plate removed, the waste bag can be taken out of the holding device
and replaced (Disposal see chapter 5.3 on page 5-7).
Pipettor wash station (see figure 2-2: position 3b)
The pipettor wash station is located next to the tip ejection station.
T: Sample rack for 20 patient samples (occupies one track).
R: Reagent rack for 18 bottles for reagents (occupies 1 tracks).
5: Reagent rack for 5 large bottles for reagents and 8 small
bottles for reagents (occupies 3 tracks).

System Basics
ELISYS QUATTRO - User Manual HUMAN 2-5
Pipetting station (see figure 2-2: position 3c)
The test plates are positioned by the system. Up to 4 plates can be positioned in this
station. A transport unit automatically moves them from the plate compartment to the
correct position where they are processed further (Loading/Unloading see chapter 5 on
page 5-1).
2.2.4 Test Plate Compartment with Bar Code
Scanner
(See see figure 2-2: position 4 or figure 2-5: position 4)
CAUTION: Laser radiation - do not stare into beam!
The bar code scanner is located on the left side at the front of the test plate compart-
ment.
The test plate compartment can be opened only on request by the software. The LED
indicator
LD lights up green.
Open the door and place the respective (bar coded) test plate with the holding frame
onto the transport unit so that both pins of the holding frame sit in the openings of the
slide. Position A1 is at the top left (see figure 2-4:). The plate is moved to the pipetting
station following the respective command on the screen.
Figure 2-4: Inserting a test plate with holding frame
From there the plate is transported - depending on the defined assay - to the various
instrument modules and then back again to the plate loading position (Loading/Unload-
ing see chapter 5 on page 5-1).
2.3 Drawer Instrument Modules
The drawer is automatically locked during a run. The green LED INSTR goes off.

2-6 ELISYS QUATTRO - User Manual HUMAN
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Figure 2-5: ELISYS QUATTRO - Bottom drawer pulled out
2.3.1 Wash Buffer Container and Waste Bottles
(See figure 2-5: position 5a)
A maximum of 3 bottles (1 x 1 litre, 2 x 2 litres) can be used for various wash buffers;
another position has to be reserved for the cleaning fluid (distilled water) to clean the
washer head.
The connection fitting consists of 4 colour-coded connection pairs: one tubing and
one level sensor each per bottle (Loading see chapter 5.1 on page 5-1).
Two waste bottles are available for the wash unit. One waste bottle contains the liquid
waste which is pumped to the waste container which is positioned below the instru-
ment. The second bottle serves as overflow protection.
2.3.2 Wash Unit for Test Plates with Window
(See figure 2-5: position 5b)
The test plates are moved into the wash unit by the system. The wash process and
the height adjustment of the washer head can be checked via the window.
2.3.3 Incubator Unit
(See figure 2-5: position 6)
4 Test plate compartment with plate transport unit (see chapter 2.2.4 on
page 2-5)
5a Wash buffer container (square) and waste bottles (round) (see
chapter 2.3.1 on page 2-6)
5b Wash unit for test plates (see chapter 2.3.2 on page 2-6)
6 Incubators (see chapter 2.3.3 on page 2-6)
7 Photometer (see chapter 2.3.4 on page 2-7)