Human HumaClot Junior User manual

!
Service Manual
!"#$%&'()*"+,'-
Cat.-No.: 18680/2

%./01/02
1 REVISIONS 4
1.1 Software 4
1.2 Service Manual (Document) 4
2 INSTRUMENT VIEWS 5
3 INSTALLATION 7
3.1 Equipment 7
3.2 Technical Data 8
3.3 Safety Standard Approvals 8
4 OPERATION INSTRUCTION 9
4.1 Warm Up 9
4.2 Test Selection 9
4.3 Stop Watch 10
4.4 Calibration 10
4.5 Default Test Values 11
4.6 Measurement 12
4.7 Measurement Stop 12
4.8 Autostart 12
5 SERVICE 13
5.1 Service Functions 13
5.2 Total Default Values 14
5.3 Temperature Adjustment 15
5.4 Optic Adjustment 16
5.4.1 OD Correction 16
5.4.2 COAG CORRECTION 16
5.4.3 OPTIC CHECK 17
5.4.4 Interface RS232 17
6 TROUBLESHOOTING GUIDE 18
7 DISASSEMBLY / ASSEMBLY 20
7.1 Disassembling the Instrument 20
7.2 Assembling the Instrument 23
8 SPARE PARTS 24
9 TECHNICAL DRAWING (3D-EXPLOSION) 25

2/26 Service Manual HumaClot Junior
03,4)5$67)&78()9&$+:

Service Manual HumaClot Junior 3/26
!"#$%$&
'()"*)+#)",+-.%*+*)#/%0)+1"$2"(+%$+%-*+)$-%#)-3+4#%5#+-5+*)#/%0%$&+-.)+621"7(5-+82$%5#9+:$+5#,)#+-5+)$*2#)
"+.%&.+()/)(+5;+4)#;5#1"$0)<+"((+="#$%$&*+"$,+#);)#)$0)*+-5+-)0.$%0"(+*";)-3+%$+-.%*+*)#/%0)+1"$2"(+12*-
>)+;5((5=),9+ ?)4"%#*+-5+-.)+%$*-#21)$-+ 1"3+ 5$(3+>)+ 0"##%),+ 52-+ >3+ -#"%$),+ 4)#*5$$)(<+ "$,+ #)4("0)1)$-
4"#-*+12*-+0514(3+=%-.+%$*-#21)$-+*4)0%;%0"-%5$*9
@.)+621"7(5-+ 82$%5#+%*+ %$-)$,),+;5#+ 2*)+ =%-.+.21"$+4("*1"9+A*+ -.)#)+ %*+ $5+B$5=$+ -)*-+ -."-+ 0"$+ 5;;)#
0514()-)+"**2#"$0)+ -."-+ 4#5,20-+,)#%/),+ ;#51+ .21"$+ >(55,+ =%((+ $5-+ -#"$*1%-+ .)4"-%-%*<+ A:CD<+ 5#+ 5-.)#
%$;)0-%52*+ ,%*)"*)*<+ "44#54#%"-)+ 4#)0"2-%5$*+ *.52(,+ >)+ -"B)$+ >3+ -.)+ %$*-#21)$-+ 54)#"-5#9+ :$+ 0"*)+ 5;
4("*1"+*4%((),+5$+-.)+%$*-#21)$-<+0()"$+=%-.+"+4"4)#+-5=)(+*5"B),+%$+EFG+>()"0.9
The !"#$%&'()*"+,'- is manufactured for HUMAN GmbH.
Company Address: HUMAN GmbH
Max-Planck-Ring 21
D-65205 Wiesbaden
Germany
Contact: Phone: [phone]
Fax: [phone] (or - 99)
e-Mail: [e-mail]
Internet: http://www.human.de

4/26 Service Manual HumaClot Junior
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;?;) 2'8(@$-7
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C 5.14 Test adaptations
For PC/AT3/DD
First release
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<7A? <787-7+D7)2'8(@$-7 <7#$-:
1 C 5.14 First edition

Service Manual HumaClot Junior 5/26
E) >/20<IB1/0)=>1J2
Figure: Front view
Figure: Rear view
Optic
!"#$%"&
Reagents
Keyboard
Display
Interface for printer
or data transmission
Sub D9 pole
Pin 2 data send
Pin 3 data receive
Pin 5 ground
DC-Input 12V/1A
Screws (2) to
open instrument
Serial No.

6/26 Service Manual HumaClot Junior
!"#$%&'()*"+,'-
Figure: The Front panel
On/Off
Optic start
Cursor up
Cursor down
Timer / Stopwatch
Enter
Test
Menu
Service / Mailfunction
Ready to use
Optic channel

Service Manual HumaClot Junior 7/26
K) >/20LMML0>./
No special precautions are necessary when starting up the !"#$%&'() *"+,'-. However, the following is
recommended:
!Place on a level surface in an area free from excessive temperature fluctuations.
!Avoid vibration during measurement.
!Protect the instrument from direct sunlight, moisture and dust.
!Check that the voltage and frequency data on the identification plate of the instrument agree
with the local power rating before starting the instrument for the first time.
The instrument is connected to the power supply by the mains cable (supplied). If obvious damage has occurred
during shipping, C')+'()"47. Contact your local distributor for replacement or repair.
K?;) 1N",5#7+(
Standard delivery package
!1 Pc !"#$%&'()*"+,'-
!1 Pc Power supply
!25 Pcs Single cuvettes
!5 Pcs Reagent tubes Ø 22.5 mm
!5 Pcs Reagent tubes with cap Ø 16.0 mm
!1 Pc Reagent adaptor Øa 23.4 / Øi 16.0 mm
!1 Pc Reagent adaptor Øa 24.1 / Øi 22.6 mm
!1 Pc Operating manual
!1 Pc Warranty card
Consumables:
Human
REF Product Content
18690 Single cuvette 500
19120 Pipette, 5 µL – 50 µL 1
19130 Pipette, 20 µL – 200 µL 1
19910/20 Pipette tips 2 - 200 µL 1.000

8/26 Service Manual HumaClot Junior
K?E) 07D3+,D$&)G$($
Dimensions (LxBxH): 205 x 150 x 75 cm
Weight: 0.51 kg (without power supply)
Ambient Temperature: 18 - 23°C
Power Supply Input : 90-264 V~ Output : 12 V, 1.0 A
Device: Micro controller board
14 Bit ADC ; on-chip controlling of LCD,
RS232, keyboard, charging,
temperature, optic.
Interface: Serial - 2400 baud, 8 bits, 1 stop, no parity
used in print or debug mode.
Optic Cell: Photometer with pulsed 400 nm LED
Variable pulse modulation
Variable detector amplification
Linear range 0.001 - 1.000 OD
Keyboard: Foil keyboard with 8 keys and 2 LED's
Display: 2 lines x 16 characters, liquid crystal
Incubation block: 1 measuring, 8 sample, 2 reagent wells; warmed to 37°C.
K?K) 2$87(O)2($+C$-C)L55-'A$&4
Manufacturer fulfills 1/)>2.)PQQ;REQQQ and 1/)>2.);KSTUR;;VEQQQ

Service Manual HumaClot Junior 9/26
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This section provides general instructions necessary for the user to achieve maximal use and benefit from the
!"#$%&'()*"+,'-. For specific test applications refer to sections in the user manual.
S?;) J$-#)I5
The first visible screen gives the operator the information of the installed software before changing to the warm-
up screen.
Info screen:
Software revision
(here: Rev. C 5.14)
Red LED lights:
It takes approx. 5 minutes
for the instrument to equilibrate to 37°C
Green LED lights:
The !"#$%&'()*"+,'- is
ready to operate.
(active test stop watch)
During the warm-up period no functions are available. The hidden service - menu can, however, be activated
during the warm-up period (refer to section "SERVICE“). The regular operator should not enter this menu.
S?E) 074()27&7D(,'+
Eight different tests can be performed on the !"#$%&'()*"+,'-
(PT, PTT, TT, FIB, FAC, DD, AT3, PC).
Enter test selection with the
:7O)X074(X?
Change active test with the
D"-4'-):7O4.
Confirm active test with the
:7O)X1+(7-X.
To alternate among the tests, press key X074(X to activate test selection, D"-4'-):7O4 to change and key 1+(7-
to confirm?
SOFTWARE C 5.14
PT: 000
PT: 000
PT: 000
FIB: 000
TEST PT:

10/26 Service Manual HumaClot Junior
S?K) 2('5)J$(D3
A stop watch function helps the operator to control the correct incubation times.
Stopwatch
To start the stopwatch press the :7O)X0,#7-X?
To stop and reset press the :7O)X0,#7-X again.
S?S) %$&,9-$(,'+
The specific parameters for the tests can be entered into the !"#$%&'()*"+,'-)and stored.
Enter calibration with the
:7O)XB7+"X?
Change parameter
with the D"-4'-):7O4.
Confirm with X1+(7-X
Change parameter (100% value = Normal value)
with the D"-4'-):7O4.
Confirm with "1+(7-X
Change parameter (50% value)
with the cursor keys
Change parameter (25% value)
with the D"-4'-):7O4
(0,0 s = no calculation to % activity)
active test is
calibrated.
?)1"#BH+ @.)+ %$42-+ 5;+ /"(2)*+ %*+ ,5$)+>3+ 4#)**%$&+ -.)+ 24I,5=$+ B)3*<+ ;%#*-+ %$+ *-)4*+ 5;+ EF+ "$,+ -.)$+ =%-.
0."$&)+5;+,%#)0-%5$+%$+*-)4*+5;+E
PT: 123
PT: PT (25%)
25,0
PT: PT (100%)
12.0
PT: ISI
1.12
PT: 000
PT: 000
PT: PT (50%)
16,7

Service Manual HumaClot Junior 11/26
S?U) G78$"&()074()=$&"74
PT: ISI = 1.10
(1) 100% (Normal) = 12.8 s (2) 50% = 16.7 s (3) 25% = 25.0 s
PTT: Normal = 30.0 s
TT: Normal = 15.0 s
FIB: (1) 300 mg/dL = 12.0 s (2) 150 mg/dL = 23.0 s (3) 75 mg/dL = 36.0 s
FAC: (1) 100% = 32.5 s (2) 10% = 60.0 s (3) 5% = 70.0 s
DD: (1) 1600 ng/ml = 150 mE (2) 200 ng/ml = 30 mE (3) 0 ng/ml = 0 mE
AT: (1) 100% = 400 mE (2) 50% = 800mE (3) 1% = 1100 mE
PC: (1) 100% = 62 mE (2) 50% = 23 mE (3) 1% = 3 mE

12/26 Service Manual HumaClot Junior
S?Y) B7$4"-7#7+(
Reconstitute reagent according to the reagent description and pre-warm (if required) in the reagent positions of
the instruments. Insert the cuvettes in the incubation area (6 positions) and pipette the required volume of
plasma into the cuvettes. To start a measurement, place the specific cuvette in the “Optic” position.
Activate the optic with the
:7O)X.5(,DX?
This screen appears only for a few seconds
and switches automatically to next screen
the measurement starts automatically with the addition
of reagent. In case of problems: press the key X.5(,DZ to start
The measurement is running.
Don’t touch the cuvette during a run!
The value in the second line shows the optical density [OD].
If a clot is found, the result is displayed
and printed (if printer is connected)
(% = 00, if 25% value is set as zero !!!)
Note: Once started a faint beeping sound is followed by a scrolling arrow. The current light absorbance (OD) can
be read on the display. Avoid contact with the cuvette while this message is shown. A beeping sound will be
heard again if a clot-reaction was detected and the result will be displayed. If a printer is attached, the result will
be also printed. If the clot reaction takes more than the maximum reading time of 300 s, the optic will stop and
display „+++.+“, which means „no clot detected“.
S?[) B7$4"-7#7+()2('5
The measurement can be canceled at any time by pressing the key X.5(,DZ again.
S?T) L"('4($-(
The measurement will start automatically when the reagent is added, if the optic is set to active. For the some
tests ( i.e. fibrinogen, thrombin ) this feature does not work properly because the change in the signal is too
small. In this case, inject the reagent quickly and with more force into cuvette and/or press the key Z'5(,D\
again.
PT: 12.8 s 000
I = 1,04 % = 00
PT: !!!! 000
0.023
PT: ACTIVE 000
PT: 000
PT: WAIT ! 000

Service Manual HumaClot Junior 13/26
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W&7$47)-7$C)(3,4)47D(,'+),+),(4)7+(,-7(O)5-,'-)(')'57-$(,+6)'+)(37)!"#$%&'()*"+,'-?)>+)'-C7-) (') 7+4"-7
$DD"-$(7)$+C)-7&,$9&7)57-8'-#$+D7)'8)(37),+4(-"#7+(^)'+&O)$"(3'-,47C)57-4'++7&^)43'"&C)57-8'-#)$+O
47-A,D7)8"+D(,'+4)'+)(37)!"#$%&'()*"+,'-),+4(-"#7+(?
U?;) 27-A,D7)_"+D(,'+4
To enter the hidden service submenu, press :7O)X0,#7-X and):7O)X1+(7-X simultaneously.
key "0,#7-" `"1+(7-"
The following service procedures can be performed on the !"#$%&'()*"+,'-R
<747()(')C78$"&()A$&"74
07#57-$("-7)$Ca"4(#7+(
.GbD'--7D(,'+
%'$6?bD'--7D(,'+
.5(,D)D37D:
27()<2EKE
Use the D"-4'-):7O4 to change and the :7O)X1+(7-X to confirm !
>+D'--7D()$Ca"4(#7+(4)@,&&),+8&"7+D7)(37)#7$4"-7#7+()4,6+,8,D$+(&Oc)d78'-7)D3$+6,+6)$+O(3,+6^)97)$@$-7
'8)(37)D'+47N"7+D74c
WAIT FOR 37°C
LOAD DEFAULT ?
NO
PT: 000

14/26 Service Manual HumaClot Junior
U?E) 0'($&)G78$"&()=$&"74
The !"#$%&'()*"+,'- can store test and system parameter permanent on board.
Calibration PT: ISI = 1.10
(1) 100% (Normal) = 12.8 s
(2) 50% = 16.7 s
(3) 25% = 25.0 s
Calibration PTT: Normal = 30.0 s
Calibration PT: Normal = 15.0 s
Calibration FIB: (1) 300 mg/dL = 12.0 s
(2) 150 mg/dL = 23.0s
(3) 75 mg/dL = 36.0 s
Calibration FAC: (1) 100% = 32.5 s
(2) 10% = 60.0 s
(3) 5% = 70.0 s
Calibration DD: (1) 1600 ng/ml = 150 mE
(2) 200 ng/ml = 30 mE
(3) 0 ng/ml = 0 mE
Calibration AT3: (1) 100% = 400 mE
(2) 50% = 800 mE
(3) 1% = 1100 mE
Calibration PC: (1) 100% = 62 mE
(2) 50% = 23 mE
(3 1% = 3 mE
Temperature °C=370 (9040)
OD Correction PT: 100
OD Correction FIB: 100
OD Correction FAC: 100
OD Correction PTT: 100
OD Correction DD: 100
OD Correction TT: 100
OD Correction AT3: 100
OD Correction PC: 100
Coag Correction PT: 100
Coag Correction FIB: 100
Coag Correction FAC: 100
Coag Correction PTT: 100
Coag Correction DD: 100
Optic check (mw): mw = approx. 11500 (range from 10xxx to 13xxx)
Integration time: approx 013 (from 011 to 020)
RS 232 Print Results (Data->RS232 = NO)

Service Manual HumaClot Junior 15/26
U?K) 07#57-$("-7)LCa"4(#7+(
The incubation block of the !"#$%&'()*"+,'- should maintain a temperature of 37,2 °C.
When the green LED is on, fill a reagent tube with 1 ml water and place it in a reagent position. Place a (digital
thermometer in the reagent tube and allow to warm up for approx. 10 min. before reading the temperature.
Example: The current temperature is 37,1°C and the digital target value is 9085.
Compare the temperature displayed by the system and the thermometer. If the temperature is different, adjust
the temperature on the !"#$%&'()*"+,'- by pressing the Up/Down cursor keys
Wait until a stable temperature of 37.0°C is displayed on the !"#$%&'()*"+,'-. Check and correct the system
temperature if not equivalent to the external thermometer.
Use the Up / Down keys to increase or decrease of temperature
SET TEMPERATURE
°C=371 (9085)

16/26 Service Manual HumaClot Junior
U?S) .5(,D)LCa"4(#7+(
U?S?;) .G)%'--7D(,'+
The optical density measured by the instrument can be corrected by a factor for each test. Therefore it is
possible to adapt other reagents.
(OD-Correction = 100 !optical density * 1.00 -> no effect)
(OD-Correction = 120 !optical density * 1.20)
OD-CORRECTION 97&'@);QQ)will cause:
- longer clotting times
- reduce sensitivity of the method ( more results will showas +++.+ s )
OD-CORRECTION $9'A7);QQ will cause:
- shorter clotting times
- increase sensitivity of the method, which can cause wrong results (short-time results !!!! )
JL</>/])R).Gb%.<<1%0>./)43'"&C)97)@,(3,+)UQ)b);UQ
special : OD-CORRECTION for fibrinogen test
Run a 300 mg/dL calibrator. The result should be close to 10 s. If it is above/below 10 s, in-/decrease the OD-
CORRECTION slightly.
U?S?E) %.L])%.<<1%0>./
With the COAG CORRECTION the instrument can correct the result for better correlation to other systems or
reagents.
Example: On another instrument a plasma sample measures with PT = 12.1 seconds; on the HumaClot Junior
the result is 11.0 seconds. To get equal results, the result has to be corrected by factor of 1.10 (+10%).
This can be done by entering COAG CORRECTION = 110 (Factor 1.10).
OD-CORRECTION
PT = ;QQ
COAG-CORRECTION
PT = ;QQ

Service Manual HumaClot Junior 17/26
U?S?K) .W0>%)%!1%e
The OPTIC CHECK is recommend, if problems with measurements arise. Remove the cuvette from optic.
The HumaClot Junior has adjusted this optic value (mw) to 11323 at an amplification (amp) of level 14.
Control range:
(ensure that no cuvette is placed in the optic)
#@R ;QQQQ)f);KQQQ
$#5R ;Qb;T
Press key “menu” to repeat the optic check, or the up/down keys to change the amplification.
U?S?S) >+(7-8$D7)<2EKE
The interface can used in two ways:
Print Mode !Results are printed
Debug Mode !Reaction curve can be portrayed on a PC
(NO !Print Mode ; YES !Debug Mode)
The interface port is set to 2400 Baud, 8 Bits, 1 Stop, No parity
W-'('D'&R))))B;))tab )/<))tab))0120))tab))21%))tab )#.G))tab))g))tab))>/<))tab))I+,()))M_
1h$#5&7)'8)$)-74"&(4)5-,+('"(R
run #01
run#02
run#03
run#04
DATA !RS232
NO
01 PT:
12.5 s
I = 1.03
02 PT:
13.2 s
I = 1.35
03 PT:
12.8 s
I = 1.22
04 PT:
12.6 s
I = 1.04
OPTIC CHECK
mw = ;;KEK Q;S

18/26 Service Manual HumaClot Junior
Y) )0<.IdM12!..0>/])]I>G1
/'(7)R L&@$O4)A7-,8O),+4(-"#7+()57-8'-#$+D7)9O)(74(,+6)$55-'5-,$(7)D'+(-'&)4$#5&74
2O4(7#)1--'-)B744$67 >+(7-5-7($(,'+)$+C)D'--7D(,A7)$D(,'+
.5(,D)_$,&"-7)c The !"#$%&'() *"+,'- is not operating in accordance with its optical
specifications.
This can happen, if
- there is not sufficient light (i.e. with very turbid reagents or lipemic samples).
Change to HUMAN reagent . Avoid extreme samples.
- there is too-intense light (i.e. direct sunlight). Protect against sunlight.
- LED is burned out. Replace device.
B7$4"-7#7+() C'74) +'(
4($-()$"('#$(,D$&&O
The change in the signal is too small.
This can happen with very clear reagents ( ie. fibrinogen, thrombin).
Try to pipette forcefully and quickly or start measurement by pressing the key
Z.W0>%\ after adding the reagent.
B7$4"-7#7+()4($-(4
,+D'--7D(&O
Signal noise and temperature drift can start the measurement incorrectly.
Stop the measurement and activate the optic shortly before starting.
<7C)27-A,D7)M1G),4)'+ Electrical power is not sufficient.
Use original power supply.
Charge battery pack, if used.
]-77+)<7$CO)M1G),4)'88 Temperature is out of range. Wait a few minutes. If the error persists, contact
technical service
07#57-$("-7)+'(
D'--7D(
Adjust the temperature.
```?`)4
X/')%&'()G7(7D(X
Always repeat the sample to verify the result
Possible reasons include the following
Clotting time is longer than 300 secs
Clotting time is shorter than 8 secs
Incorrect reagent
Fibrinogen level of sample is below 100 mg/dL ( i.e. sample dilutions )
Air bubbles
Debris in cuvette
Sample improperly collected
OD-Correction is set to zero
%&'((,+6)(,#7)('')43'-( Always repeat the sample to verify the result.
Possible reasons include the following:
Incorrect reagent
Air bubbles
Debris in cuvette
Sample improperly collected
Optic is incorrectly adjusted
Action:
Use recommended materials
Decrease OD Correction slightly
Increase OD Range

Service Manual HumaClot Junior 19/26
2O4(7#)1--'-)B744$67 >+(7-5-7($(,'+)$+C)D'--7D(,A7)$D(,'+
%&'((,+6)(,#7)('')&'+6 Always repeat the sample to verify the result.
Possible reasons includes the following:
Incorrect reagent
Air bubbles
Debris in cuvette
Low fibrinogen level
Sample improperly collected
Optic is incorrectly adjusted
Action:
Use recommended materials
Increase OD-Correction slightly
Decrease OD-Range