HYRIS bCUBE User manual

HYRIS System™
Quick-Start Guide
(English)
Version 2.1
Hyris Srl
Corso Garibaldi, 60
20121 Milano, Italy
Phone: [phone]
Mail: [e-mail]
Other contacts:
Hyris Ltd
Lower Ground Floor, One George
Yard, EC3V 9DF, London UK
Phone: [phone]
Mail: [e-mail]
Hyris Asia Pac
38 19 Cecil Street
#04-01 - The Quadrant at Cecil,
Singapore (049704)
Phone: [phone]
Mail: [e-mail]
Hyris Americas
Los Gatos
Phone: [phone]
Mail: [e-mail]

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Product info
[e-mail]
Ordering info
[e-mail]
bCUBE (REF: bCUBE 2.0)
bCUBE3 (REF: H0001)
bAPP (REF: bAPP, H00001)
bPANEL (REF: H00002)
Visit our website at
www.hyris.net
Hyris Srl
Corso Garibaldi, 60 20121
Milano (MI) Italy

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Copyright ©2022 Hyris S.r.l. All rights reserved.
Reproduction of this document, either in printed or electronics form, is prohibited without the permissionof Hyris S.r.l.
Information in this document is subject to change without notice. Hyris S.r.l. assumes no responsibility for any incorrect
information that may appear in this document.
Notice:
HYRIS S.R.L.DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,EXPRESSED OR IMPLIED,INCLUDING BUT NOT LIMITED TO THOSE OF
MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.IN NO EVENT SHALL HYRIS S.R.L.BE LIABLE,WHETHER IN CONTRACT,TORT,WARRANTY,OR
UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL,INCIDENTAL,INDIRECT,PUNITIVE,MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION
WITH OR ARISING FROM THIS DOCUMENT,INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
Trademarks:
HYRIS SystemTM, HYRIS bCUBETM, HYRIS bCUBE3TM, HYRIS bKITTM, HYRIS bAPPTM, HYRIS bDATATM and HYRIS bPANELTM
are a trademark of Hyris S.r.l.
Microsoft® and Windows® are registeredtrademarks of Microsoft Corporation. FAM™, HEX™ and CY5™ are a trademark
of Thermo Fisher Scientific or its subsidiaries in the U.S. and certainother countries.
Technical Support Contacts:
Hyris S.r.l
Corso Garibaldi 60, 20121 Milano(MI) –ITALY
Phone: [phone]
Mail: [e-mail]
Website: https://www.hyris.net/

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INDEX
About this Manual 5
HYRIS bCUBETM 6
Packing content.............................................................................................................................................................................6
Hyris bCUBETM Instrument overview.................................................................................................................................................7
Status LEDs...................................................................................................................................................................................8
FIRST SETUP 10
PC settings..................................................................................................................................................................................10
HYRIS bCUBETM Connection...........................................................................................................................................................10
INTERACTING WITH HYRIS bCUBETM: HYRIS bAPPTM AND HYRIS bPANELTM 13
HYRIS bAPPTM interface ................................................................................................................................................................13
System Requirements..................................................................................................................................................................13
Access the HYRIS bAPPTM............................................................................................................................................................13
Create a new analysis...................................................................................................................................................................15
General information 15
Create a new analysis: the samples 18
Create a new analysis: the Cartridge Layout 18
Analysis details 20
GENERAL tab 20
STEPS tab....................................................................................................................................................................................22
STEPS tab –advanced features 23
RESULTS Tab 24
HYRIS bPANELTM interface.............................................................................................................................................................26
System Requirements 26
Installation 26
Main view 26
Settings: Log in/Sync and HYRIS bCUBETM connection 27
New analysis creation 28
Run the analysis 29
Old analyses view 30
HYRIS bCUBETM operative procedure 31
APPENDIX-1: Set HYRIS bCUBETM Wi-Fi from bCUBEs menu 32
APPENDIX-2: Custom Recipe 33
APPENDIX-3: HYRIS Cartridge Preparation 34
Load samples ..............................................................................................................................................................................34
Load the cartridge inside HYRIS bCUBETM........................................................................................................................................34
Removing the cartridge................................................................................................................................................................34
Technical support / Service 36
Support Contacts.........................................................................................................................................................................36

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About this Manual
This manual is referred to bCUBE (REF: bCUBE 2.0) and bCUBE3 (REF: H0001). All references to bCUBE will be referred also
to bCUBE3, unless explicitly specified.
For HYRIS bCUBETM connectivity and functionalities, refer to the Hyris bCUBETM –User Manual document.
For HYRIS bPANELTM offline use, refer to the Hyris bPANELTM –User Manual document.
For the online use of the instrument using HYRIS bAPPTM, please read the HYRIS bAPPTM –User Manual guide.
The information contained in this Guide is proprietary. No part of this document can be copied or reproduced in any form
without written permission of Hyris S.r.l.
The information contained in this manual is subject to change/revision without notice.
This Guide has not to be intended as User Manuals substitute.
In the event the Quick Start Guide should be ruined, incomplete or inadequate, please contact Hyris S.r.l. in order to
immediatelyrestore or replace the incompliant Guide.
The official version of the Quick Start Guide, of which Hyris S.r.l. is directly responsible, is theEnglish version. For countries
in which languages other than Englishare spoken, the official Guide is the one in the Englishversion. Hyris S.r.l. does not
undertake any responsibility for any translations in other languages made by distributors or users.
The observance of the operating procedures and of the warnings described in this Guide is a basic requirement for the
correct working of the equipment and to guarantee the operator’s safety.
The Guide must be read in every part in front of the equipment before using it, in order to become familiar with the
operating procedures, the commands, and the precautions for a correct and safe usage.
The Quick StartGuide should be kept, complete and readable in every part, in a safe place, and, at the same time, it should
be rapidly accessible to the user when using the equipment.

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HYRIS bCUBETM
Packing content
The following items will be found in the provided box:
•HYRIS bCUBETM (Figure 1a)
•Ethernet cable (Figure 1b)
•Power Supply including European and North American power cords (Figure 1c).
Characteristics:
oInput: 100 ~ 240 V c.a. 1.4A 50/60Hz
oOutput: 12V DC 5A
oPower: 60W
•USB stick with HYRIS bPANELTM software installer and User Manuals (Figure 1d)
Note: Please check the presence of all these items. Remove the items carefully from the shipping box and inspect
them for any external damage. If any of the parts is missing ordamaged, contact the manufacturer prior to
installation.

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Hyris bCUBETM Instrument overview
Status LEDs
Access Port
Instrument Base
Upper Venting Holes
Power-on/Power-off
Button
Ethernet Plug
Power Supply
Plug

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Description of the features highlightedin the pictures above:
•Access Port Slider: opened to insert/remove the cartridge inside Hyris bCUBETM. To be kept closed during
operations.
•Access port: insert the cartridge here. Always push the cartridge up to the end of the port.
•Thermal Block: precisely controls the temperature of the cartridge and contained samples. Heating/Cooling is
obtained with a Peltier element.
•Detection block: features highbrightness LEDs withdichroic filters for fluorophores excitation. Detection is
obtained thanks to a CMOS sensor with dichroic filters matched to fluorophores emissionwavelengths.
•Top venting holes: allows air exchange between the inside of the Instrument and the ambient
•Power supply Plug: insert here the 12 VDC adapter jack
•Power-on/Power-off button: press shortly to wake up the instrument from Stand-By. When the machine is
running, long press (about 5 seconds) to safelypower down the bCUBE
•Ethernet Plug: insert here the Ethernet cable for Windows PC / Network communication
•Status LEDs: Give informationabout the status of the instrument
Status LEDs
The status of the bCUBE and its functioning are communicated to the user by the different color combinations of the Status
LEDs on the top of the device.
•bDATA LED: corresponding to the symbol, it indicates the connection status to Hyris server.
Ready color: green
When Ready color is on, HYRIS bCUBETM is connected to HYRIS bDATATM
•Thermal LED: corresponding to the symbol, it indicates if the cartridge is hot or not.
Ready color: orange
When Ready color is on, the internal temperature of the HYRIS bCUBETM is not hot. If
cartridge is inserted, it can be safelyhandled.
•Connection LED: corresponding to the symbol, it indicates the internet connection state of the HYRIS
bCUBETM
Thermal Block
Access Port
Detection Block
Access Port
Slider

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Ready color: blue
When ready color is on, Hyris bCUBETM is connected to a network
For a graphical representation, the symbol (from left to right: bDATA, Thermal and Connection LEDs) will be
displayed in this document when color code explanation is required.
Some examples are reported in the following table:
bDATA
LED
Thermal LED
Connection
LED

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LEDs Colours
DESCRIPTION
INTERPRETATION
•bDATA LED steady green
•Thermal LED steady orange
•Connection LED steady blue
•HYRIS bDATATM connection OK
•Cartridge not hot
•Network connection OK
•bDATA LED steady green
•Thermal LED blinking orange and red
•Connection LED steady blue
•HYRIS bDATATM connection OK
•WARNING: Cartridge hot
•Network connection OK
•bDATA LED blinking green and yellow
•Thermal LED steady orange
•Connection LED blinking red and yellow
•Missing HYRIS bDATATM connection
•Cartridge not hot
•Missing network connection
•bDATA LED blinking green and yellow
•Thermal LED steady orange
•Connection LED steady blue
•Missing HYRIS bDATATM connection
•Cartridge not hot
•Network connection OK. HYRIS bCUBETM
can be used locally
•bDATA LED multicolour
•Thermal LED multicolour
•Connection LED multicolour
•Experiment is running on HYRIS
bCUBETM
•bDATA LED off
•Thermal LED off
•Connection LED off
•HYRIS bCUBETM off
FIRST SETUP
PC settings
PC has to be connected to a stable internet connection.
HYRIS bCUBETM Connection
In order to properly work, the following steps have to be respected:
1. Make sure the Hyris bCUBETM is set on a flat and stable surface
2. Make sure to have correctly provided the condition explained in the PC settings section
3. Connect Hyris bCUBETM and PC to the same LAN according to the following configuration
Note: not all the Status LEDs colour combinations are shown. For further information, please read the
HYRIS bCUBE - User Manual.