HYRIS bCUBE 2.0 User manual

Hyris Ltd
_________________________________________
Hyris Headquarters
Lower Ground Floor, One George Yard,
EC3V 9DF, London UK
Phone: [phone]
Mail: [email protected]
_________________________________________
Hyris Research Center
Corso Garibaldi, 60
20121 Milano, Italy
Phone: [phone]
Mail: [email protected]
_________________________________________
Hyris Asia Pac
38 Ang Mo Kio Industrial Park 2 #02-07A
569511 Singapore
Phone: [phone]
Mail: [email protected]
_________________________________________
www.hyris.net
London, September 2019
bCUBE® 2.0 Quick Start Guide
Hyris Platform guide
Version 1.2.5

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Copyright ©2019 Hyris Ltd. All rights reserved.
Reproduction of this document, either in printed or electronics form, is prohibited without the
permission of Hyris Ltd.
Information in this document is subject to change without notice. Hyris Ltd assumes no responsibility
for any incorrect information that may appear in this document.
Notice:
bCUBE® 2.0 is only for research purpose and for all other fields except for in vitro diagnostics.
HYRIS LTD DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,EXPRESSED OR IMPLIED,INCLUDING BUT NOT
LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.IN NO EVENT SHALL HYRIS LTD BE LIABLE,
WHETHER IN CONTRACT,TORT,WARRANTY,OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL,INCIDENTAL,
INDIRECT,PUNITIVE,MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT,
INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
Trademarks:
bCUBE® and bDATA® are registered trademarks of Hyris Ltd.
Microsoft® and Windows® are registered trademarks of Microsoft Corporation. FAM™, HEX™ and
ROX™ are a trademark of Thermo Fisher Scientific or its subsidiaries in the U.S. and certain other
countries.
Technical Support Contacts:
Hyris Ltd –Italian Branch
Corso Garibaldi 60, 20121 Milano (MI) –ITALY
Phone: [phone]
Mail: [email protected]
Website: https://www.hyris.net/

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Index
Hyris bCUBE® 2.0....................................................................................................................................4
Box Opening.........................................................................................................................................4
Device Overview ..................................................................................................................................5
Status LEDs...........................................................................................................................................6
First set up..............................................................................................................................................8
PC settings............................................................................................................................................8
bCUBE® 2.0 Connection .......................................................................................................................8
Interacting bCUBE® 2.0: bAPP and bPANEL .........................................................................................10
bAPP interface....................................................................................................................................10
Access the bAPP...............................................................................................................................10
Create a New Analysis: general information...................................................................................11
Create a new analysis: the cartridge ...............................................................................................13
Analysis details ................................................................................................................................15
bPANEL 2.0 interface .........................................................................................................................18
Installation.......................................................................................................................................18
Main view ........................................................................................................................................18
Settings: Log in/Sync and bCUBE® 2.0 connection..........................................................................19
New analysis creation......................................................................................................................20
Run the analysis...............................................................................................................................21
Old analyses view ............................................................................................................................21
Flow Chart ............................................................................................................................................23
Appendix-1: Set bCUBE® 2.0 Wi-Fi from bCUBEs® menu ....................................................................24
Appendix-2: Custom Recipe .................................................................................................................25
Appendix-3: Cartridge Preparation ......................................................................................................26
Load samples......................................................................................................................................26
Load/Unload the cartridge to/from bCUBE® 2.0...............................................................................26

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Hyris bCUBE® 2.0
Box Opening
The following items will be found in the provided box:
obCUBE® 2.0 (Figure 1a)
oEthernet cable (Figure 1b)
oPower Supply including European and North American power cords (Figure 1c).
Characteristics:
-Input: 100 ~ 240 V c.a. 1.4A 50/60Hz
-Output: 12V DC 5A
-Power: 60W
oUSB stick with Hyris bPANEL software installer and User Manuals (Figure 1d)
Note: Please check the presence of all these items. Remove the items carefully from the
shipping box and inspect them for any external damage. If any of the parts is missing or
damaged, contact the manufacturer prior to installation.

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Device Overview

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Description of the features highlighted in the pictures above:
oAccess Port Slider: opened to insert/remove the cartridge inside bCUBE® 2.0. To be kept
closed during operations.
oAccess port: insert the cartridge here. Always push the cartridge up to the end of the port.
oThermal Block: precisely controls the temperature of the cartridge and contained samples.
Heating/Cooling is obtained with a Peltier element.
oDetection block: features high brightness LEDs with dichroic filters for fluorophores
excitation. Detection is obtained thanks to a CMOS sensor with dichroic filters matched to
fluorophores emission wavelengths.
oTop venting holes: allows air exchange between the inside of the Instrument and the
ambient
oPower supply Plug: insert here the 12 VDC adapter jack
oPower-on/Power-off button: press shortly to wake up the instrument from Stand-By. When
the machine is running, long press (about 5 seconds) to safely power down the bCUBE®
oEthernet Plug: insert here the Ethernet cable for Windows PC / Network communication
oStatus LEDs: Give information about the status of the instrument
Status LEDs
The status of the bCUBE® and its functioning are communicated to the user by the different colour
combinations of the status LEDs on the top of the device.
obDATA®LED: corresponding to the symbol, it indicates the connection status to Hyris
server.
Ready colour: green
When Ready colour is on, bCUBE® 2.0 is connected to bDATA®
oThermal LED: corresponding to the symbol, it indicates if the cartridge is hot or not.
Ready colour: orange
When Ready colour is on, the internal temperature of the bCUBE® 2.0 is not hot. If

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cartridge is inserted, it can be safely handled
oConnection LED: corresponding to the symbol, it indicates the internet connection state
of the bCUBE® 2.0
Ready colour: blue
When ready colour is on, bCUBE® 2.0 is connected to a network
For a graphical representation, the symbol (from left to right: bDATA®, Thermal and
Connection LEDs) will be displayed in this document when colour code explanation is required.
Some examples are reported in the following table:
LEDs Colours
DESCRIPTION
INTERPRETATION
-bDATA® LED steady green
-Thermal LED steady
orange:
-Connection LED steady
blue:
-bDATA® connection
OK
-Cartridge not hot
-Network connection
OK
-bDATA® LED steady green
-Thermal LED blinking
orange and red
-Connection LED steady
blue
-bDATA® connection
OK
-WARNING: Cartridge
hot
-Network connection
OK
-bDATA® LED blinking
green and yellow
-Thermal LED steady
orange
-Missing bDATA®
connection
-Cartridge not hot

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-Connection LED blinking
red and yellow
-Missing network
connection
-bDATA® LED blinking
green and yellow
-Thermal LED steady
orange
-Connection LED steady
blue
-Missing bDATA®
connection
-Cartridge not hot
-Network connection
OK. bCUBE® 2.0 can
be used locally
-bDATA® LED multicolour
-Thermal LED multicolour
-Connection LED
multicolour
-Experiment is running
on bCUBE® 2.0
-bDATA® LED off
-Thermal LED off
-Connection LED off
-bCUBE® 2.0 off
First set up
PC settings
PC has to be connected to a stable internet connection.
bCUBE® 2.0 Connection
In order to properly work, the following steps have to be respected:
1. Make sure the bCUBE® 2.0 is set on a flat and stable surface
2. Make sure to have correctly provided the condition explained in the PC Set-up section
3. Connect one end of the Ethernet cable to the bCUBE® 2.0 and the other to the PC according
the following configuration
Note: not all the Status LEDs colour combinations are shown. For further information,
please read the Owner’s Manual.

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4. Power on the PC. Wait until PC is ready
5. Connect the PC to internet. Make sure internet connection is stable
6. Power on the bCUBE® 2.0. Status LEDs colours:
7. bCUBE® will start booting. Status LEDs colours:
8. Wait until Connection Status LED colour is steady blue: or
If LEDs colours are different from the above mentioned, try to repeat the steps. Otherwise,
check the Owner’s Manual
9. Access the bAPP (see the Access the bAPP section) and go to the bCUBEs® section. Select the
desired bCUBE® by checking the Serial number of the machine (see the Owner’s manual).
The “Connected” flag should be a green icon, indicating that the bCUBE® is connected to a
network. An action menu should be show
10. Click in the button to access the “Local settings page”, then go to the “Network” section.
Select one of the “Available WIFI Networks” and enter the related password, then click on
. See the Owner’s manual to perform WPS connection to the router or to manually add
a network. The saved Wi-Fi should immediately appear in the “Saved WIFI Networks”
column.
11. When all the desired Wi-Fi networks are saved, go back to the bAPP and unplug the Ethernet
cable from the bCUBE®. After some seconds, the Status LEDs should show the Hyris Ready
colours . If not, check the troubleshooting section on the Owner’s manual.
Note: Status LEDs may display different colours while bCUBE® 2.0 is looking for network
connection. If no connection is detected, the following LEDs colours may be displayed
after a minute of waiting time: . If the problem persists, see the Owner’s Manual.

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Interacting bCUBE® 2.0: bAPP and bPANEL
bCUBE® 2.0 can be controlled and monitored with two Hyris tools: bAPP and bPANEL.
obAPP: it’s a web app software and there’s no need to install it on the PC. Ready-to-use
recipes or custom recipes (see dedicated section) can be used to create and run analyses on
the available devices according to the account permissions. Account and devices
management are possible by this application. It can be run on PC or Smartphone. Internet
connection is mandatory.
obPANEL: this software interface is designed for offline usage of the bCUBE® device, while
also providing internet data sharing when connection is available. bPANEL 2.0 can start new
analyses by using the already existing recipes (created on bAPP).
bAPP interface
Access the bAPP
1. Make sure to have properly followed the instruction described in the bCUBE® 2.0
Connection section.
If bCUBE® 2.0 is ready for operations, open the internet browser (Google Chrome
browser recommended) on the PC and type https://bapp.hyris.net in the address bar. Then
press enter to access the bAPP.
2. The Home window will be shown. The screen is mainly divided in two sections:
oMain Menu: list of available bAPP sections:
-Home: Welcome Window. Log-in credentials required when starting a new
session on bAPP
-Analyses: all the available analyses are listed in this section. New analyses can be
created
-bKITs: bKITs are reagent sets used to analyse a specific genetic sequence. The
bKITs developed and sold by Hyris Ltd are listed in this section
-Recipes: a “Recipe” is a detailed list of steps and settings that the bCUBE® has to
execute while performing an analysis. New recipes can be customized from this
menu (see Appendix-2: Custom Recipe). All the available recipes are listed here
-Swarms: “Swarm” is used to define a collector of analyses and recipes. User can
have access to multiple swarms according to his permissions. Visible Swarms are
listed in this section
-Users: list of available users and relative permissions (see Owner’s Guide for
further information)
-bCUBEs®: list of available bCUBEs® with relative extra information (see Owner’s
Guide for further information)
-Certificates: list of requested certificates.