IBA FABian User manual

Document No.: 010
Version: 9
Modification Date: 2020-11-04 © 2020 IBA GmbH
Instruction Manual

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 2of 39
Product Specifications
Item:
FABian®
Product Version:
Laboratory Version
Software Version:
FABian firmware 1.0
Item-No.:
6-6100-260
Delivery Content:
FABian®, PSU & Power Cable, 50 ml Tube Holder, Syringe
Adapter 10 ml, Instruction Manual, SD-Card, Return Form,
Decontamination Declaration
Revision Index / -Date
Rev. 9, 2020/11/04
IBA GmbH
Bosestr. 4
04109 Leipzig
Germany
Phone: [phone]
Fax: [phone]
E-Mail: [e-mail]
Homepage: www.iba-lifesciences.com
On behalf of the IBA GmbH, the instruction manual has been designed and engineered by Cell.Copedia
GmbH.
©
This instruction manual may not be copied in whole or in part without the authorization of IBA
GmbH. The figures in this instruction manual may differ from the actual design and are not
displayed on full scale (1:1).

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 3of 39
Index
0Preface....................................................................................................................................................... 5
1Explanation of Symbols.............................................................................................................................. 5
2Product Description and Technical Data.................................................................................................... 6
2.1 General Product Information and Unit Design.................................................................................... 6
2.2 Delivery Content.................................................................................................................................. 7
2.3 Technical Data .................................................................................................................................... 7
3For your Safety........................................................................................................................................... 8
3.1 Warning Signs and Security Level...................................................................................................... 8
3.2 Intended Use and Foreseeable Improper Operation .......................................................................... 8
3.3 Target Group, Staff Qualifications....................................................................................................... 9
3.4 Personal Protective Equipment........................................................................................................... 9
3.5 Safety Instructions for Commissioning................................................................................................ 9
3.6 Operating Conditions of the Product................................................................................................... 9
3.7 Safety Instructions For Operation ..................................................................................................... 10
3.8 Safety Instructions for Cleaning, Maintenance ................................................................................. 10
3.8.1 Cleaning..................................................................................................................................... 10
3.8.2 Maintenance .............................................................................................................................. 11
3.9 Decommissioning, Dismantling, Disposal......................................................................................... 11
4Transport and Storage ............................................................................................................................. 12
4.1 Transport........................................................................................................................................... 12
4.2 Storage.............................................................................................................................................. 12
4.3 Storing and Protecting between Usage Periods............................................................................... 12
5Prepare FABian® for Use......................................................................................................................... 13
5.1 Select Installation Place.................................................................................................................... 13
5.2 Prepare Installation ........................................................................................................................... 13
5.3 Install Unit.......................................................................................................................................... 14
6Operation of FABian®.............................................................................................................................. 16
6.1 Commissioning.................................................................................................................................. 16
6.2 Normal Operation.............................................................................................................................. 16
6.2.1 Inserting the Syringe.................................................................................................................. 16
6.2.2 Inserting the Tube Holder .......................................................................................................... 18
6.2.3 Inserting the Tubes .................................................................................................................... 18
6.2.4 Operating Keys and Program Selection .................................................................................... 20
6.2.5 Program Run.............................................................................................................................. 20
6.2.6 Stopping the Program, Removal of the Sample and of the Syringe.......................................... 22
6.3 Fault Repair....................................................................................................................................... 24
6.4 Technical Support ............................................................................................................................. 24
7Maintenance and Cleaning....................................................................................................................... 25
7.1 Cleaning............................................................................................................................................ 25
7.2 Maintenance and Repair by the Customer Service .......................................................................... 26
8Provided Accessories, Consumables, Spare Parts ................................................................................. 27
8.1 Consumables .................................................................................................................................... 27
8.2 Spare- and Replacement Parts......................................................................................................... 28
9Information on Special Tools, Devices, Materials.................................................................................... 31
10 Decommissioning, Dismantling, Disposal............................................................................................. 34
11 Annexes................................................................................................................................................ 35

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Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 4of 39
Tables of Figures
Figure 1 –Overview Unit Design....................................................................................................................... 6
Figure 2 –Insert SD-Card ............................................................................................................................... 14
Figure 3 –Power Connection to the Device.................................................................................................... 15
Figure 4: FABian® Syringe.............................................................................................................................. 17
Figure 5: Insertion of the Syringe (1)............................................................................................................... 17
Figure 6: Insertion of the Syringe (2)............................................................................................................... 18
Figure 7 - Notch for Locking Mechanism......................................................................................................... 18
Figure 8 - Tube-Contents ................................................................................................................................ 19
Figure 9 –Operating Keys............................................................................................................................... 20
Figure 10 –Program Selection –Green Illuminated Keys.............................................................................. 20
Figure 11 –Program Selection........................................................................................................................ 21
Figure 12 - Program A Is Running................................................................................................................... 21
Figure 13 –Removal of the Target Product: Tube 9, 10 und 11..................................................................... 23
Figure 14: Image of a Tube............................................................................................................................. 27
Figure 15: Image of a Syringe......................................................................................................................... 27
Figure 16 –Image of the Tube Holder, SD-Card, PSU & Power Cable.......................................................... 29
Figure 17: Image of Syringe Adapter incl. Tool............................................................................................... 30
Figure 18: Removal of the Screws From the Bottom Part of the Adapter....................................................... 31
Figure 19: Unscrewing the Screws From the Upper Part of the Adapter........................................................ 31
Figure 20: Removal of the Syringe Adapter .................................................................................................... 32
Figure 21: Insertion of the Bottom Part of the Adapter.................................................................................... 32
Figure 22: Installation of the Bottom Part of the Adapter ................................................................................ 32
Figure 23: Insertion of the Upper Part of the Adapter ..................................................................................... 33
Figure 24: Tightening the Upper Part of the Adapter ...................................................................................... 33
List of Tables
Table 1 –Explanation of the Appliance Switches and Symbols ....................................................................... 5
Table 2 –List of Supplied Parts......................................................................................................................... 7
Table 3 –Overview Technical Data................................................................................................................... 7
Table 4 - Checklist........................................................................................................................................... 13
Table 5 –Key Functions.................................................................................................................................. 20
Table 6 –List of Consumables........................................................................................................................ 27
Table 7 –List of Spare parts and Accessories................................................................................................ 28
Abbreviations
AC Alternating Current PSU Power Supply Unit
°C Degree Celsius SELV Safety Extra Low
Voltage
DC Direct Current UPS Uninterruptible Power
Supply
EU European Union V Volt
F Fahrenheit W Watt
g Gram
Hz Hertz
Ib Pound
kg Kilogram
m Meter
ml Milliliter
mm Millimeter
PBS Phosphate-Buffered Saline

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 5of 39
0 Preface
In order to ensure a safe and appropriate usage, read the instruction manual and further product-
related documents carefully and keep them in safe custody for future use!
This instruction manual contains important information, which must be read and understood in full
before the device is used. A precondition for safe operation is the observance of all safety and
handling instructions specified in this instruction manual.
This instruction manual is part of the product, must always be kept near the device and should be
available for the personnel at any time.
In case of loss or damage of the instruction manual, please request a replacement.
In addition to the instructions in this manual, the regulations of the employers’ liability insurance
association for health protection and industrial safety apply.
Exclusion of Liability:
The manufacturer will not assume any liability, if:
-the regulations in this manual are not being followed,
-the device has been used improperly or has not been used for the specified purpose,
-spare parts, accessories or consumables are being used, that are not approved by the manufacturer
-the device has not been operated by trained and instructed personnel,
-the housing has been opened or removed,
-the device has been repaired or maintained by a non-authorized person.
Furthermore, users are forbidden to modify FABian®. If there is still any modification, the product warranty
will cease. IBA GmbH is not responsible for possible consequences caused by a modification and will be
exempted from all kinds of customer care commitments. In this case, the safety of FABian® cannot be
guaranteed anymore. The manufacturer will not accept any liability for damages resulting from modifications.
1 Explanation of Symbols
Table 1 –Explanation of the Appliance Switches and Symbols
Symbols und Appliance Switches
Type Plate (placed on the bottom side of the
device)
Do not dispose of the device with normal
household garbage!
Symbol Protection Class III (SELV)
On / Off-Switch →Device is turned on and off;
Initialization of the device
Start-Stop-Button →Program is started / stopped;
device is in transport position
Program Selection Keys

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 6of 39
2 Product Description and Technical Data
2.1 General Product Information and Unit Design
Figure 1 –Overview Unit Design
FABian® is an automatic benchtop device for the selection of cells from a suspension (e.g. blood or other
single cell suspensions) and is designed for research use only. The system may be operated exclusively in a
laboratory environment by qualified and specially trained personnel. The selection process is performed with
the help of disposable products: max. 11 tubes on a round tube holder and the special FABian®-syringe are
necessary to gain the pure, minimal manipulated and non-activated target product. The operation of
FABian® is simple and intuitive. By pushing the on/off-Switch on the right side of the device FABian® starts
the initiation program. For the operation, FABian® is equipped with 5 operating keys –4 program selection
keys and one start-stop-button. After the insertion of the syringe and the tubes and after the program is
chosen, the device is passing the specific selection steps. Depending on the chosen program the whole
selection process takes about 30-90 minutes (duration depending on chosen program) to provide purified
target cells which can be removed from the tubes afterwards.

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 7of 39
2.2 Delivery Content
Table 2 –List of Supplied Parts
Amount
Item-No.
Description
Additional Information
1
6-6100-260
FABian® Device
Spare Part
1
6-6104-050
50 ml Tube Holder
Spare Part
1
6-6105-010 /
6-6106-010
Syringe Adapter 10 ml (already mounted
in the cover)
Spare Part
1
6-6103-000
SD-Card with preinstalled program
selection
Spare Part
1
6-6101-000
PSU
Spare Part
1
6-6102-000
Power Cable
Spare Part
1
FABian® Instruction Manual, english
Keep in safe custody!
1
Return Form
to complete by the customer
1
Decontamination Declaration
to complete by the customer
2.3 Technical Data
Table 3 –Overview Technical Data
Dimensions / Weight (Mass, Capacity, Power)
Measurements
Width
198 mm
Depth
315 mm
Height
230 mm
Operation Height
455 mm
Weight (net)
3840 g (Basic Unit 3088 g, Tube Holder 752
g)
Power Supply Data
Power Supply Unit
Input Voltage
90…264 V AC
Frequence
47 - 63 Hz
Power
max. 20 W
Input Voltage Device
24V DC
Safety Devices
integral, self-resetting
Protection Class (according to
DIN 61140)
III (SELV)
Protection Type (IP-Code)
IP30
Environmental Conditions
Environment
Use indoors only!
Ambient Temperature
Operation
+10 °C (50 F) - +35 °C (95 F)
Storage
+10 °C (50 F) - +35 °C (95 F)
Humidity
No condensating humidity
Operation
0% - 75%
Storage
0% - 75%
Air Pressure
Usage up to 2.000 m above sea level
Application Parameters
Device Temperature
25 °C
Maximum Load
Tubes
11 Tubes à 30 - 50 ml
Syringe
1 Syringe à 10 ml

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 8of 39
3 For your Safety
3.1 Warning Signs and Security Level
Security Level
DANGER
Will lead to serious injuries or
death
WARNING
Can lead to serious injuries or
death
CAUTION
Can lead to moderate or minor
injuries
ATTENTION
Can lead to property damage
Note
Indicates useful information
3.2 Intended Use and Foreseeable Improper Operation
FABian® is an automatic device for the selection of cells from a suspension (e.g. blood or other single cell
suspensions) with the help of a syringe and tubes and is designed for research use only. The selection
device is intended only for the purposes determined and any other application (e.g. therapeutic application)
or any further purposes shall be considered as improper.
IBA GmbH shall not accept liability for any damage arising as a result of the above mentioned modifications.
The intended use also includes the attention of all references of the instruction manual, the compliance with
maintenance and inspection work as well as the observation of the installation instructions and of the
cleaning and maintenance notes.
Residual Risks
Although the device is constructed and qualified in accordance with the current state of the art and with
recognized safety regulations and standards, the operation of the device involves a certain amount of risks.
Residual risks result from crushing fingers by the piston- and syringe lift stages in the event of non-
compliance with the safety regulations as well as from electric shock, if you operate the device with a power
supply unit which is not indicated here.
Faults which may adversely affect the safety shall be promptly notified to the IBA GmbH.
According to the fault type it will be decided individually, if the error can be resolved by the user or by the
service personnel of the IBA GmbH.
Warning Signs
General Danger
Electric Shock Danger
Risk of Crushing
Explosion Danger
Biological Hazard
Note
WARNING!
➔The non-compliance with the described safety regulation may result in health
damages or may lead to damages to the device. The manufacturer cannot be
made liable for any damage caused by the non-compliance with the safety
regulation.

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 9of 39
WARNING!
Risk of Biological and Chemical Contamination by Used Reagents
➔
If biological or chemical hazardous materials are involved, please observe the
relevant warnings and protection provisions for those materials and wear
appropriate personal protective equipment.
3.3 Target Group, Staff Qualifications
FABian® may be operated exclusively for commercial use by trained and qualified personnel, who have fully
read and understood the instruction manual. The user must be familiar with the unit´s functions. This
knowledge can be achieved by reading the instruction manual and by additional information- and training
offers by the IBA GmbH.
By purchasing the first devices we recommend training courses for a safe and appropriate usage.
3.4 Personal Protective Equipment
The device shall only be used with laboratory protective equipment. The users must observe the
occupational safety regulations of the operating company as well as the national regulations for occupational
safety.
In case of usage of biological or chemical hazardous materials protective gloves, protective clothing and
safety glasses shall be worn.
3.5 Safety Instructions for Commissioning
The power cable must be routed in such a way that it does not represent a tripping hazard and that no
damages can be caused by other devices. Use only the original power cable.
3.6 Operating Conditions of the Product
FABian® is designed to be used exclusively indoors. For a safe usage a dry condition is required (see
ambient temperature and humidity chapter 2.3), e.g. a ventilated laboratory.
DANGER!
Electric shock Caused by Device Damages or Power Cable
➔
Activate the unit only if it is secured that the device and the power cable are
intact.
➔Only start up devices, which are professionally installed or properly maintained.
WARNING!
Danger Causes by Improper Power Supply
➔
FABian® may only be operated with the voltage specified on the power supply
unit. Connect the device to a properly earthed and fused power socket only.
WARNING!
Danger of Injuries and Device Damages as a Result of Incorrect Operation by Non-
qualified Personnel
➔
Misapplications of FABian® and the non-compliance with security regulations
may result in injuries caused by e.g. electric shock or crushing.
➔
An improper operation of the device can lead to total loss of the target product,
accessories and consumables and can cause serious damages to the device.
Explosion Danger
➔Do not use FABian® in rooms susceptible to explosions.
➔Do not use FABian® with radioactive, explosive or corrosive materials.
DANGER
!

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Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 10 of 39
3.7 Safety Instructions For Operation
Protection Devices, Emergency Stop
An emergency stop, which is installed inside or outside of the laboratory, is desirable for disconnecting the
power supply in case of failures. We recommend the connection to an in-house emergency power unit that
will take over the power supply in case of an electricity failure. This can often be identified by green labeled
power sockets.
Precautions during operation
For the operation of the device movable components are required. The tube holder and the inserted tubes
can fall from accidental collisions. To avoid risks and accidents, do not touch the device during operation.
We recommend placing the device as far away as possible from table edges, to prevent a falling of the
device and personal injury. Do not under any circumstances connect an injection needle to the syringe for
device operation. There is a risk of puncturing the fingers, if you accidentally place your fingers under the
injection needle during operation.
Should your fingers nevertheless become trapped between the device levels, you can release your fingers
with your other hand or with the help of another person by lifting the device level manually.
Never leave the device unattended while in operation. Please stop the process in case of device damages,
abnormal sounds, odors or any other irregularities; switch the device off, pull out the power plug and call the
service technician. When unplugging the power plug hold it by the plug only and remove it carefully.
3.8 Safety Instructions for Cleaning, Maintenance
3.8.1 Cleaning
The device must always be cleaned after use.
DANGER!
Life-Threatening Voltage by Opening the Device
➔
Make sure, that the housing is intact and closed, so that no risks can arise from
the inside of the device.
➔
It is not allowed to remove the cover of the device. All work on the device should
be performed exclusively by service personnel of the IBA GmbH.
DANGER!
Electric Shock Caused by Damp Device and Leaking Liquids
➔
Switch off the device and disconnect the power supply previous to the cleaning
process.
➔Ensure that no liquids leak into the inside of the device.
➔
Only if the device and the power cord are completely dry, connect the device to
the power supply and switch it on again.
ATTENTION
Damaged Component Parts Can Lead to Injuries and to a Loss of the Target
Product
➔
Periodically check all component parts of the device and make sure that they
work perfectly.
➔
Damaged tubes and syringes can lead to damaged samples / target product and
can cause damages to the device.
➔
Be sure that there are no component parts in use, whose expiry date has passed.
CAUTION!
Crushing of Fingers by Device Levels
➔
Do not touch the device during commissioning and operation. Do not place your
fingers between the device levels. Your fingers may get between the device
levels and the process might be interrupted.

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WARNING!
Risk of Biological and Chemical Contamination by Used Reagents
➔
If hazardous materials were used, a careful decontamination of the device must
be carried out. It is strictly forbidden to use contaminated devices or accessories.
➔
The decontamination and disposal of consumables are performed according to
the applicable provisions.
3.8.2 Maintenance
Before each use, the device must be subjected to a visual inspection. If device damages are visible or the
function of FABian® is restricted, it must be maintained.
3.9 Decommissioning, Dismantling, Disposal
FABian® should be stopped immediately when a safe and proper function is no longer guaranteed. The
same applies to all accessories and spare parts.
It is prohibited to dismantle the device. Return the device to IBA GmbH or properly dispose of the device.
Do not dispose of the device with normal household garbage!
For more detailled information, see chapter 10.
WARNING
The user is not permitted to perform the maintenance
➔
If the device has been maintained by a non-authorized person, any claim under
warranty and any service will cease.

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4 Transport and Storage
4.1 Transport
The manufacturer is responsible for the first transport. The operator is however obliged to immediately check
the device upon receipt. If externally detectable transport damage is found refuse the delivery or accept it
under reserve. Specify the suspected damage and inform the IBA GmbH immediately.
The following instructions should be followed when transporting FABian®:
-transport the unit in its original packaging only
-do not place the carton upside down or on the side.
4.2 Storage
If the device needs to be stored until the commissioning, please note the following instructions:
- Store product in dry conditions. The relative humidity should not exceed 75 %.
- Device must not be stored outdoors. The floor of the storage room must be flat and dry.
- Do not expose the device to direct sunlight and moisture.
- Storage temperature should be between +10 °C (50 F) and +35 °C (95 F).
- Avoid mechanical shocks and damages. Store the device safe and stable; if necessary install
additional safety elements.
- If you store the device without original packaging, ensure a dust-free environment.
The used consumables must be stored in a cool place. Please note the storage conditions as indicated in the
instruction manual of the respective reagents.
4.3 Storing and Protecting between Usage Periods
If you do not use FABian® for a longer period, please ensure a safe and dust-free storage. The device
should be cleaned immediately after use (see chapter 7.1) and should be subsequently kept in the original
packaging. Even between usage periods the environmental conditions, as described in chapter 4.2, apply,
especially the provisions concerning temperature and space.

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5 Prepare FABian® for Use
5.1 Select Installation Place
The installation place for FABian® must meet the following criteria:
-power supply according to type plate available
- stable, flat and resonance free (laboratory) table, on which the device with a weight of 3,85 kg (basic
unit & tube holder) can be positioned safely
- put the device on a slip-resistant, non-flammable and water-resistant surface
- keep at least 30 cm of space on every side of the device; be aware that the device gets 23 cm
higher during operation; to avoid an additional external heating it is necessary to choose a well
ventilated environment which is protected against direct sunlight
- be sure, that the device does not come into contact with open flame; do not expose the device to
moisture and condensation water and ensure that the position of the device is not subjected to
vibrations and extreme temperature fluctuations
- ambient temperature is between +10 °C (50 F) - 35 °C (95 F)
- relative humidity of the device environment should not exceed 75 %.
5.2 Prepare Installation
To prepare the installation process it is important to select an appropriate place (see 5.1) and to remove the
package. While unpacking proceed as follows:
1. Put the box with the lid on top –open it.
2. Remove the first small box which contains single parts and accessories.
3. Remove the first styrofoam cover and lift the device out of the box.
Please keep the original packaging material for transport purposes, for a complaint and / or for the
storage between usage periods.
After unpacking, check the components for external integrity. The device should be delivered together with
the following components:
Table 4 - Checklist
Component
Included in scope of delivery
FABian® Device
(please tick off)
PSU / Power Cable
SD-Card
50ml Tube Holder
Syringe Adapter 10 ml (already
mounted in the cover)
Instruction Manual
Return Form
Decontamination Declaration
WARNING!
Danger in Case of Failure
➔
Provide an emergency switch close to the unit to disconnect the power supply in
case of failure.

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FABian® is free-standing device and does not need an additional holder. Due to the low weight the
configuration and connection of the device can be performed by one person.
5.3 Install Unit
For the installation of the unit proceed as follows:
1. Put the device safely and stable on a flat (laboratory) table (see chapter 5.1 Select Installation
Place).
2. Locate the device in such a way that the program selection keys are pointing towards you.
3. Take the provided SD-card and insert it with the backside facing upwards into the corresponding slot
at the lower left of the basic unit (see figure 2) until you hear a clicking sound. The SD-card has to
remain in the unit during operation.
Figure 2 –Insert SD-Card
4. Make sure that the AC line voltage is the same as the operating voltage of the PSU before you
connect it up. If the ratings for voltage (V) and maximum current (A) do not match, the device must
not be connected.
5. Plug the low-voltage plug of the power cable in the low-voltage socket of the device. The low-voltage
socket is located on the bottom side of the device (see figure 3).
WARNING!
Danger Caused by Incorrect Power Supply
➔An incorrect power supply may result in malfunctions or in permanent damages.
ATTENTION!
Damages of FABian® or of Devices in the Immediate Environment
➔
To ensure a secure process and to avoid damages of devices in the immediate
environment, keep a safety distance of at least 30 cm during operation according
to EN 61010-2-020.
ATTENTION!
Device Damages Caused by Improper Transport and Condensation
➔
After the transport from a cooler to a warmer environment condensation is
possible. Please wait at least 3 hours, before connecting the device to the power
supply. Any transport-induced temperature differences could be compensated.
ATTENTION!
Defective Selection Process by Premature Removal of the SD-Card
➔
Do not remove the SD-card from the device during operation. Otherwise the
selection process can be interrupted.

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Figure 3 –Power Connection to the Device
6. Plug the power cable in a properly installed and fused power socket.
7. Make sure that the power cable is not subjected to tensile or pressure forces.
DANGER!
Electric shock Caused by Device Damages or Power Cable
➔Please check plug and power cable for damage before connection.
➔
Damaged parts must never be used for the connection and must be replaced by
original parts.

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6 Operation of FABian®
6.1 Commissioning
Before commissioning the device, the entire body as well as any accessories, which could compromise the
operational safety, should be checked thoroughly for any defects. Please check the power supply and the
power cable for damage regularly. If there is any damage, you should not use the device under any
circumstances. Disconnect the power plug and replace the damaged parts by original spare parts.
Connecting
Check, that no used syringe has remained from the last selection process
Please make sure that the cables are properly extended on the floor along the device and
ensure that the cables are routed away from moving parts (e.g. under lift stage I & II).
1. Activate the device with the on/off-switch. To do this, please press the silver button on the lower right
side of the device.
2. The device is initializing, which is recognizable by significant noises, by up- & down-movements of
the two lift stages as well as by the movement of the roundabout towards the zero position. All
switches flash in orange color.
3. The initialization is finished when the operation noises are gone and the roundabout is on zero
position. The program selection keys flash green.
Before first commissioning a check is required to ensure the completeness and functionality and to recognize
possible damages. This check includes a functional and visual inspection according to a checklist, which you
will find in Appendix A of the instruction manual. Enter your results into the checklist and keep it in your
product documentation for the length of the warranty.
The system is now ready for operation.
6.2 Normal Operation
6.2.1 Inserting the Syringe
The device has been positioned in such a way that the program selection keys are pointing towards you.
Only insert the syringe when the device is switched on (recognizable by the flashing of the program selection
keys), the device has initialized completely and is standing still.
Before use, ensure that the roundabout is on zero position.
ATTENTION!
Danger of Leaking Liquids by Premature Insertion of the Syringe
➔
If you insert the syringe too early –before the device is initialized –the liquids in
the syringe can be dispensed prematurely.
The device can become dirty, which can lead to the invalidity of the selection.
ATTENTION!
Danger of a Faulty Selection Process and of Leaking Liquids
➔
If the cable is not routed properly, it can slip between the device levels and
interrupt the process.
➔The leaking of liquids is possible.
WARNING!
Crushing of Fingers by Device Levels
➔
Do not touch the device during commissioning. Do not place your hand between
the device levels, your fingers can be crushed and the process can be aborted.

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 17 of 39
1. To insert the syringe remove it from the packaging and remove existing safety elements. The design
of the syringe and the designation are shown in figure 4.
Figure 4: FABian® Syringe
2. Insert the syringe into the round opening of the syringe adapter below the syringe lift stage with the
piston facing upwards (see figure 5). Push the syringe as far as possible towards the lid.
3. Ensure that the syringe piston is correctly placed in the
designated notch. Then push the syringe with the syringe body in the
direction of the housing until it locks into place and until the syringe
body is firmly attached in the adapter (see figure 6).
ATTENTION!
Danger of Damages by Turning the Mini-Column
➔Do not turn the mini-column to avoid a loosening.
➔
An opening of the syringe can lead to a loss of materials and liqiuds can leak into
the device.
WARNING!
Use Only Original Fabian® Syringes (Without Needle), Risk of Injury
➔FABian® may be operated exclusively with the original syringe.
➔
It is forbidden to use other syringes equipped with needles. These kinds of
syringes are not applicable for the selection process and can puncture your
fingers and other body parts.
Figure 5: Insertion of the Syringe (1)

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 18 of 39
6.2.2 Inserting the Tube Holder
Carefully place the tube holder on the device and fill it with the tubes afterwards. If you are already practiced
in the handling you can place an already filled tube holder on the device. Please place the tube holder in
such a way, that the opening is pointing toward the rear (zero position).
With the help of a notch (see figure 7) the tube holder has to be accurately locked to the roundabout.
Figure 7 - Notch for Locking Mechanism
6.2.3 Inserting the Tubes
Now you can load the tube holder with the tubes. The order of the tubes is already fixed in the manual of the
consumable kit and of great importance. Please comply with the requirements of your selected program. An
interchanging of the tubes can invalidate the selection.
ATTENTION!
Interruption of the Process by Incorrect Insertion of the Tube Holder
➔
Be sure, that the tube holder is properly inserted. If the tube holder is not properly
pointing toward the rear, interruptions of the process and device damages can
appear.
Figure 6: Insertion of the Syringe (2)
ATTENTION!
Risk of Injury from Sharp Edges of the Tube Holder
➔Maintain a distance as far as possible from the device and from moveable parts.
➔Use the device with caution, to avoid injuries.

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 19 of 39
ATTENTION!
Device Damages Caused by Spilling Liquids from Tubes or Syringe
➔Make sure, that the device is completely clean and dry before continuing.
➔Spilled liquids can cause device damages.
FABian® is equipped with preset programs. An exemplary program setting is shown in the following figure,
however it may vary. For that reason please refer to the instruction manual of the consumable kit for the
exact order.
In the example setting shown here, the following ingredients and tubes are necessary and to arrange as
follows:
4 x wash solution (PBS)
2 x biotin
1 x Fab-fragment
1 x blood / cells
Figure 8 - Tube-Contents
If you accidently spill liquids during the insertion of the tube holder or the tubes, an ordinary cleaning of the
device is sufficient:
1. Switch off the device.
2. Disconnect the device from the power supply.
3. Perform a comprehensive cleaning of the device and of all accessories according to the
specifications in chapter 7.1.
FABian® is now ready for program start.
WARNING!
Danger of Interchanging Tubes
➔
Every interchanging of the tubes can lead to an incorrect selection process and
can make the used reagents unusable.
➔
Your research results are being distorted, if the reagents / the tubes are
interchanged.

Product: FABian® Item-No.: 6-6100-260
Instruction Manual, Revision 9 Doc.-No.: 010 Date of Issue: 2020/11/04 Page 20 of 39
6.2.4 Operating Keys and Program Selection
The operating keys (see fig. 9) are used for program selection and to start and stop the unit.
Figure 9 –Operating Keys
Please refer to the following chart for the description of the different key functions.
Table 5 –Key Functions
Switches &
Buttons
Function
On / Off-Switch
Turns FABian® on and off and initializes the device; by pressing the on / off-switch
the emergency stop can be activated.
GO
1. Start-Stop-Button: Pushing the GO-button confirms the program selection; the
process starts
2. Pause-Button: Pushing the pause-button during operation interrupts the program;
it continues by pushing the GO-button again
3. By a long pressure of the GO-button during operation the program will be stoped
and the device turns back into its zero position
A, B
Program Selection Keys
1, 2
Program Selection Keys
1 + A
By a long pressure of this button-combination the device runs back into the compact
transport position
6.2.5 Program Run
1. If the syringe is inserted and the tube holder is loaded and locked the selection process can be
started. All program selection keys flash green (see figure 10).
Figure 10 –Program Selection –Green Illuminated Keys