ibidi Universal Fit 10918 User manual

10918 ibidi Heating System, Universal Fit for 1 Chamber
Version 2.2
Instruction Manual
ibidi Heating System, Universal Fit
Lid
I 42.1
S[ 42.0] Run
Plate Glass
37.0
37.0 N.C.
37.0
Setup
N.C.

ibidi Heating System, Universal Fit
Version 2.2 (May 23, 2019) 2 Instruction Manual

ibidi Heating System, Universal Fit
Contact
ibidi GmbH
Lochhamer Schlag 11
82166 Gr¨ afelfing
Germany
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-mail: [email protected]
Internet: ibidi.com
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ibidi Heating System, Universal Fit
Contents
1 Preamble 6
1.1 Introduction ........................................... 6
1.2 Safety Symbols ......................................... 6
1.3 Nomenclature .......................................... 7
1.4 Specifications .......................................... 7
1.5 Disclaimer ............................................ 10
1.6 Safety Considerations ...................................... 10
1.7 Regulatory Statement ...................................... 11
1.8 Limited Warranty ........................................ 12
1.9 Transporting the ibidi Heating System, Universal Fit .................... 12
1.10 Repairing the ibidi Heating System, Universal Fit ..................... 13
1.11 Waste Disposal – WEEE/RoHS Compliance Statement .................. 13
1.11.1 EU Directive WEEE ................................... 13
1.11.2 EU Directive RoHS ................................... 13
2 Intended Use 14
3 Principle 14
4 Equipment 15
4.1 Components of the ibidi Heating System, Universal Fit .................. 15
4.2 Combination Options ...................................... 16
4.3 Temperature Controller ..................................... 17
4.4 Heated Lid ............................................ 18
4.5 Heated Plate ........................................... 19
4.6 Heating Inserts ......................................... 20
4.6.1 Heating Insert µ–Slide ................................. 20
4.6.2 Heating Insert µ–Dish 35mm, high and Heating Insert µ–Dish 35mm, low ...... 20
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ibidi Heating System, Universal Fit
5 Operation 22
5.1 Installation and Connection of the Parts ........................... 22
5.2 Start the ibidi Heating System, Universal Fit ........................ 23
5.3 Setting Parameters in the Front Display ........................... 23
5.3.1 Run/Stop Mode .................................... 24
5.3.2 Incubation Parameters ................................. 24
5.3.3 Setup Menu ....................................... 24
5.4 Temperature Adjustment in the Sample ........................... 26
5.5 Sample Preparation ....................................... 27
6 IncuControl Software 28
7 Maintenance 29
7.1 Disinfection and Cleaning ................................... 29
7.2 Influence of Ambient Temperature and Ventilation ..................... 29
8 Troubleshooting 30
8.1 Focus not Stable ......................................... 30
8.2 Evaporation Is too High .................................... 30
8.3 Condensation Inside the Stage Top Incubator ........................ 30
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ibidi Heating System, Universal Fit
1 Preamble
1.1 Introduction
This manual is your guide to using the ibidi Heating System, Universal Fit for cell culture experi-
ments on an optical microscope. It instructs first-time users how to use the instrument, and serves as
a reference for experienced users.
Before using the ibidi Heating System, Universal Fit, please read this instruction manual carefully
and make sure that the contents are fully understood. This manual should be easily accessible to the
operator at all times during instrument operation. If this manual gets lost, order a replacement from
www.ibidi.com.
To ensure safe operation, the ibidi Heating System, Universal Fit must only be operated with the
supplied components and according to the instruction manual.
1.2 Safety Symbols
Note that the signal words WARNING,CAUTION and NOTE have specific meanings in this man-
ual. Do not proceed beyond a signal word until you have performed the indicated actions.
WARNING! A potentially hazardous situation which, if not avoided, could result in se-
rious injury or even death. Warning messages in the text are displayed in a
gray shaded box.
CAUTION A potentially hazardous situation which, if not avoided, could result in minor
or moderate injury. It is also used to alert against damaging the equipment
or the instrument.
NOTE Additional information to help achieve optimal instrument and assay perfor-
mance.
Symbols on the product identification label and back panel of the device:
CE Marking: This symbol indicates the product’s compliance with EU legis-
lation.
This label is positioned on the back of the device and prompts you to read the
manual before using the device.
Product disposal: The symbol indicates that this product must be recy-
cled/disposed of separately from other household waste. See page 13 for
details.
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ibidi Heating System, Universal Fit
1.3 Nomenclature
Temperature Controller Heated Lid Heated Plate
1.4 Specifications
Only operate the Heating System in the range of the specifications given below:
Table 1 – Specifications of the ibidi Heating System, Universal Fit
Electrical Specifications Power Supply
Protection class I
International protection marking
(IEC 60529)
IP 20
Overvoltage category II
External power supply AC 100-240 V, 50/60 Hz, 2 A
Input line voltage Temperature
Controller
DC 24 V, 6.67 A, 160 W
Output voltage to channel 1
(Heated Lid), Universal Fit
DC 10 V, max. 5 A
Output voltage to channel 2
(Heated Plate), Universal Fit, for 1
Chamber
DC 12 V, max. 5 A
Operating and Storage Conditions
Operating site Indoor use only
Operating temperature 18-30°C/64-86°F (min 5°C/9°F less than set temperature)
Operating humidity max. 80% relative humidity (RH)
Operating altitude max. 2000 m (atmospheric pressure 800-1060 hPa/11.6-15.4
psi)
Storage conditions -5-50°C/23-122°F, humidity <60% relative humidity (RH)
Outer Dimensions and Characteristics of the Components
Temperature Controller 90 mm × 170 mm × 230 mm
Weight: 1720 g/3.8 lbs
Heated Lid 19 mm × 85.5 mm × 127.5 mm (134.5 mm with cover ridge)
Length of cable: 1.5 m
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ibidi Heating System, Universal Fit
Table 1 – (continued)
Connector to Gas Incubation: Female Luer Lock
Heated Plate 12 mm × 85.5 mm × 127.5 mm
Observation area: 40 mm × 82 mm
Length of cable: 1.5 m
Heated Plate assembled with
Heated Lid
Height: 25.5 mm
Weight: 330 g/0.46 lbs
Heating Inserts all 47 mm × 97 mm
Heating Insert 35 mm Dish high Height without blank holder: 8 mm
Height with blank holder: 16-18 mm
Observation area: 22 mm
Weight insert: 50 g/0.11 lbs
Weight blank holder: 72 g/0.16 lbs
Heating Insert 35 mm Dish low Height without blank holder: 8 mm
Height with blank holder: 13-15 mm
Observation area: 22 mm
Weight insert: 50 g/0.11 lbs
Weight blank holder: 47 g/0.11 lbs
Heating Insert Slides Height without blank holder: 8 mm
Height with blank holder: 13-15 mm
Observation area: 49 mm × 22 mm
Weight insert: 42.5 g/0.94 lbs
Weight blank holder: 38.5 g/0.85 lbs
USB cable Length: 1.8 m
Power supply cable Length: 2.0 m (power supply to wall)
Length: 1.2 m (power supply to device)
Temperature Control Range
Heated Lid Ambient temperature (min. 18°C) to +45°C
Accuracy: ±0.2°C (at sensor location)
±5°C (entire heated glass)
Heated Plate Ambient temperature (min. 18°C) to +45°C
Accuracy: ±0.2°C (at sensor location)
±0.5°C (entire heated plate)
Recommended Temperatures before Adjustment
See Section 5.4
USB Interface
Connector type USB 2.0 Connector Type B
Recommended USB cable Tripp Lite UR022-006 (shielded)
Driver FTDI VCP driver
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ibidi Heating System, Universal Fit
Table 1 – (continued)
Microscope Requirements
Working distance condenser ≥26 mm
Stage holder Holder for standard multi-well plates
Optical Properties Glass Lid
Glass Selected HQ Floatglass
Thickness of the glass plate 1.1 mm
Refractive index glass nD= 1.520 (588 nm)
ITO coating Thickness: 100 nm
Passivation layer Thickness: 20-25 nm
Refractive index ITO with passiva-
tion layer
1.95
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ibidi Heating System, Universal Fit
1.5 Disclaimer
• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of
product use.
• The contents of this manual are subject to change without notice for product improvement.
• This manual is considered complete and accurate at publication.
• This manual does not guarantee the validity of any patent rights or other rights.
• If an ibidi software program doesn’t function properly, this may be caused by a conflict from
another program operating on the computer. In this case, take corrective action by uninstalling
the conflicting product(s).
• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
1.6 Safety Considerations
WARNING!
• Only operate the ibidi Heating System, Universal Fit with the supplied components.
• Only use the cables and plugs delivered with the system. The power plug of the control unit
must be inserted in an outlet with a ground (earth) contact.
• Do not replace detachable power cables by power cables with inadequate specifications. By
violating these instructions you risk electric shock and fire.
• Only use extension cables that have a protective ground wire.
• Do not operate the ibidi Heating System, Universal Fit under conditions that pose a risk of
explosion, implosion, or the release of gases. Only operate the ibidi Heating System, Universal
Fit with aqueous solutions.
• Do not operate a damaged ibidi Heating System, Universal Fit. If the housing seems damaged
or something is rattling inside the controller, contact the ibidi service hotline for repair.
• Some accessible parts of the Heated Plate and Heated Lid can reach temperatures up to 55°C.
Avoid touching the temperature-controlled parts of the system when you have set the Temper-
ature Controller to high temperatures.
CAUTION
• Ensure that the external power supply is easily accessible. The ibidi Heating System, Universal
Fit must be installed in a manner such that none of its components hinders access to the external
power supply.
• Immediately replace damaged cords, plugs, or cables to avoid risk of personal injury or damage
to the instrument.
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ibidi Heating System, Universal Fit
• Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser-
vice the ibidi Heating System, Universal Fit.
• The external power supply should not be brought into contact with moisture. If the housing is
damaged, the external power supply should not be used.
• Avoid strong magnetic fields and sources of high frequency. The ibidi Heating System, Univer-
sal Fit might not function properly when located near a strong magnetic field or high frequency
source.
• Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
• Avoid dust and corrosive gas. Do not install the ibidi Heating System, Universal Fit where it
could be exposed to high levels of dust or to outside air or ventilation outlets.
• Install the ibidi Heating System, Universal Fit in a horizontal and stable position, such as a
table, bench, or desk upon which the instrument is installed.
• Install the ibidi Heating System, Universal Fit in a location that enables easy access for mainte-
nance.
• Do not place heavy objects on the instrument.
• The heated glass plates of the incubation chamber can break on mechanical impact. If so, the
glass shards can lead to injuries if handled.
• Be aware that when switched on, a 10 V DC voltage is applied to the underside of the glass on
the Heated Lid. Do not touch the underside or put it in contact with anything conductive. This
could cause a short circuit that may damage the Temperature Controller and/or the Heating
Devices.
1.7 Regulatory Statement
The ibidi Heating System, Universal Fit has been designed, produced and tested in compliance with
the European standard DIN EN 61010-1 (IEC 61010-1, ”Safety requirements for electrical equipment
for measurement, control and laboratory use”). Furthermore it meets the IEC 61326-1 (”Electrical
equipment for measurement, control and laboratory use - EMC requirements”) and CISPR 11 (”Inter-
national Standard for electromagnetic emissions (disturbances) from Industrial, Scientific and Medi-
cal (ISM) Equipment”) standards .
The device carries the CE mark.
The ibidi Heating System, Universal Fit meets the Low Voltage Directive 2014/35/EU and the
EMC Directive 2014/30/EC.
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ibidi Heating System, Universal Fit
1.8 Limited Warranty
Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects in the
product are found during this warranty period, ibidi will repair or replace the defective part(s) or
product free of charge.
This warranty does not apply to defects resulting from the following:
1. Improper or inadequate installation.
2. Improper or inadequate operation, maintenance, adjustment, or calibration.
3. Unauthorized modification or misuse.
4. Use of unauthorized tubing or fluidic connectors.
5. Use of consumables, disposables, and parts not supplied by an authorized ibidi distributor.
6. Corrosion due to the use of improper solvents, samples, or due to surrounding gases.
7. Accidents beyond ibidi’s control, including natural disasters.
This warranty does not cover consumables, such as cell culture chambers and dishes, tubes, fluidic
connectors, reagents etc.
The warranty for all parts supplied and repairs provided under this warranty expires on the warranty
expiration date of the original product.
1.9 Transporting the ibidi Heating System, Universal Fit
The weight of the Temperature Controller is approx. 1.7 kg/3.8 lbs. Moving the Temperature Con-
troller during operation can pose a risk of personal injury or damage to the instrument.
For transport, switch off the Temperature Controller and then disconnect the heated components
from the controller. Carry the devices carefully and avoid mechanical shocks.
WARNING!
Hot surface (max. 55°C)! Do not touch Heated Lid and Heated Plate when hot. Always disconnect
the instrument from the power supply before transport and leave the instrument to cool down
for approx. 5 minutes.
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ibidi Heating System, Universal Fit
1.10 Repairing the ibidi Heating System, Universal Fit
For inquiries concerning repair service, contact the ibidi service personnel and provide the model
name and serial number of your system.
ibidi GmbH
Service Hotline: [email protected]
CAUTION
Do not try to repair the ibidi Heating System, Universal Fit by yourself. Disassembly of the ibidi
Heating System, Universal Fit is not allowed. Disassembly poses a risk of personal injury or
damage to the devices. Contact ibidi service personnel if there is a need to disassemble a device.
1.11 Waste Disposal – WEEE/RoHS Compliance Statement
The European Union (EU) has enacted two directives, the first on product recycling (Waste Electrical
and Electronic Equipment, WEEE) and the second on limiting the use of certain substances (Restric-
tion on the use of Hazardous Substances, RoHS).
1.11.1 EU Directive WEEE
The ibidi Heating System, Universal Fit must be disposed of in compliance with the WEEE Directive
2012/19/EC.
This symbol on the product is in accordance with the European Union’s Waste Electrical and Elec-
tronic Equipment (WEEE) Directive. The symbol indicates that this product must be recycled/dis-
posed of separately from other household waste. It is the end user’s responsibility to dispose of this
product by taking it to a designated WEEE collection facility for the proper collection and recycling of
the waste equipment. The separate collection and recycling of waste equipment will help to conserve
natural resources and protect human health and the environment. For more information about recy-
cling, please contact your local environmental office, an electrical/electronic waste disposal company
or distributor where you purchased the product.
1.11.2 EU Directive RoHS
The ibidi Heating System, Universal Fit meets the requirements set forth in the RoHS Directive
2011/65/EU and the additional terms as specified in RoHS Directive 2015/863/EU.
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ibidi Heating System, Universal Fit
2 Intended Use
The ibidi Heating System, Universal Fit is a stage top incubator for live cell imaging that fits onto
inverted microscope stages with a universal mounting frame for multiwell plates. The geometry of
the chamber, with a heated glass plate above the sample, provides a platform for keeping microscopy
slides and dishes at a constant temperature during an experiment on a microscope.
For full control of the incubation environment, the ibidi Heating System, Universal Fit can be com-
bined with the ibidi Gas Incubation System to regulate the CO2and O2concentrations as well as the
humidity.
The modular concept of the Heating Inserts enables the use of a variety of microscopy labware rang-
ing from 35 mm dishes to regular slides.
3 Principle
Physiological Conditions in Live Cell Imaging
The Heated Lid and Heated Plate are designed to keep cells on-stage at 37°C in microscopy slides
and dishes (Figure 1). To achieve this, the glass top of the Heated Lid and the Heated Plate are
actively heated by the Temperature Controller. The Heated Lid prevents condensation effects inside
the incubation system (Figure 2).
Heated Plate
Heated Lid
40°C37°
Gas mixture,
Humidity
Humidity Sensor
Humidity
O2
Levels
CO
2
Levels
Temperature
Cell culture vessel
Figure 1 – Schematic cross-sectional view of the ibidi Heating System, Universal Fit.
Heated Plate
Heated Lidibidi solution:
40 °C
37 °C
Heated Plate
Unheated Lid
34 °C
37 °C
Figure 2 – The Heated Lid prevents condensation effects on the lid of the cell culture vessel.
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ibidi Heating System, Universal Fit
4 Equipment
This section provides a brief overview over all parts of the ibidi Heating System, Universal Fit in-
cluding a description of the characteristics.
4.1 Components of the ibidi Heating System, Universal Fit
The components of the ibidi Heating System, Universal Fit are listed below.
Description Drawing
Temperature Controller
Heated Lid with electrical cable and D-sub connector to
connect to the Temperature Controller
Heated Plate with electrical cable and D-sub connector to
connect to the Temperature Controller
Heating Insert µ–Dish 35mm, high*
Heating Insert µ–Dish 35mm, low*
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ibidi Heating System, Universal Fit
Description Drawing
Heating Insert µ–Slide*
Heating Insert LabTek*
Temperature Adjustment Set:
1 temperature sensor
1 perforated ibidi µ–Slide 8 Well
1 perforated ibidi µ–Dish 35mm, high
1 perforated ibidi µ–Dish 35mm, low
USB cable to connect the Temperature Controller with a
computer
Country specific power cord to connect the external power
supply to the wall socket
External power supply for the Temperature Controller
USB flash drive with IncuControl software
*One Heating Insert of your choice is delivered with the ibidi Heating System, Universal Fit. If
needed, more Heating Inserts can be ordered separately.
4.2 Combination Options
The parts of the ibidi Heating System, Universal Fit are combined as shown in Figure 3. The Heated
Lid and Heated Plate fit on an inverted microscope stage equipped with a universal mounting frame
for multiwell plates. They are connected to the Temperature Controller.
The ibidi Heating System, Universal Fit can be combined with the Gas Mixer unit, that provides CO2
and O2(optional) control and a defined humidified atmosphere. Detailed information on the Gas
Mixer unit is given in the Gas Mixer instructions.
To hold several geometries of Slides and Dishes, the Heated Plate can be equipped with different
Inserts (Section 4.6): Insert for µ–Dish 35mm, high, Insert for µ–Dish 35mm, low, Insert for µ–Slides, and
Insert for LabTek™ chambered coverglass.
For parallelization of experiments, the Heated Plate for 4 µ-Slides provides a platform to observe
4 Slides in parallel. Detailed information is given in the instructions of the ibidi Heating System,
Universal Fit for 4 µ-Slides (#10927).
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ibidi Heating System, Universal Fit
Figure 3 – Overview of the parts of the ibidi Heating System, Universal Fit with options to combine
4.3 Temperature Controller
The Temperature Controller is designed to control the different heated components (four channels
available).
The front display shows the set values (S) and the current values (I). The settings can be adjusted via
the control buttons (Section 5.3).
If you wish to control the Temperature Controller via PC, use the IncuControl software (Section 6).
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ibidi Heating System, Universal Fit
Figure 4 – Front view of the Temperature Controller.
The LEDs on the front indicate the status of the channels, connection to the power supply, and USB
connection (Figure 4).
Control LEDs Channel 1-4
LED off Channel inactive
LED on Channel active
LED fast blinking Channel error
All plugs for the electrical connections are integrated into the rear of the Temperature Controller
(Figure 5). The setup of the connections is shown in Section 11.
USB
Power supply
External
themperature
sensor
On/Off switch
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Type:
Serial number:
Production date:
ibidi Temperature Controller
ibiTC2-001
11/2018
ibidi GmbH
Am Klopferspitz 19
D-82152 Martinsried
Germany
[phone]
www.ibidi.com
Heated Lid
Heated Plate
Heated Glass Bottom
Heated Plate, 4 Slides
4 control channels
Figure 5 – Rear view of the Temperature Controller.
4.4 Heated Lid
The Heated Lid provides excellent optical quality, and also allows for the use of all standard mi-
croscopy techniques, including differential interference contrast (DIC). Due to the height of the lid,
we recommend using condensers with a working distance of ≥26 mm.
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ibidi Heating System, Universal Fit
The upper glass part of the Heated Lid is heated. The electrical cable is connected to the Temperature
Controller. The Heated Lid fits exactly and securely onto the Heated Plate.
For gas incubation, the Heated Lid is equipped with inlets for the gas flow and the humidity sensor.
Detailed information on the Gas Incubation System is given in its instruction manual.
Figure 6 – Heated Lid
4.5 Heated Plate
The Heated Plate provides the base for the ibidi Heating System, Universal Fit. The lower part of the
Heated Plate fits into any microscope stage with a universal mounting frame for multiwell plates.
The electrical cable (1.5 m) is connected to the Temperature Controller.
Figure 7 – Heated Plate, top view
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ibidi Heating System, Universal Fit
4.6 Heating Inserts
The Heating Insert holds the sample and fits into the Heated Plate. The Insert is held in place by
strong neodymium magnets. Use the handles to place/remove the insert into/from the Plate of the
ibidi Heating System, Universal Fit.
The Heating Inserts have a lower part (insert) that fit into the Heated Plate, and an upper part (holder)
to press the sample down. The two–part inserts have two functions that both rely on the force of the
integrated magnets. The first function is to hold the Slides or Dishes firmly in position, so as to avoid
displacement during microscope stage movements. The second is to create a tight contact between
the Slides or Dishes and the metallic insert, to maximize the heat transfer and ensure stable heating
of the sample. The Heated Plate passively heats the inserts.
WARNING!
Heated Plate, Heated Lid, and Heating Inserts all contain strong neodymium magnets! Please
contact us for a non-magnetic system, if permanent magnetic fields are detrimental to your ex-
periment.
4.6.1 Heating Insert µ–Slide
The Heating Insert µ–Slide fits 75 mm × 25 mm µ–Slides (e.g. all ibidi µ–Slides).
Figure 8 – Heating Insert µ–Slide
4.6.2 Heating Insert µ–Dish 35mm, high and Heating Insert µ–Dish 35mm, low
The Heating Insert µ–Dish 35mm, high fits only ibidi µ–Dish 35mm, high, the Heating Insert µ–Dish 35mm, low
fits only ibidi µ–Dish 35mm, low
Center the µ–Dish in the insert before you put the holder on.
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