ibidi ibidi Pump System User manual

Version 2.6
Instruction Manual
ibidi Pump System
ibidi.com

ibidi Pump System
Contact
ibidi GmbH
Lochhamer Schlag 11
82166 Gr¨ afelfing
Germany
Phone: +49 89 / 520 46 17 - 0
Fax: +49 89 / 520 46 17 - 59
E-mail: [email protected]
Internet: ibidi.com
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ibidi Pump System
Contents
1 Preamble 7
1.1 Introduction ........................................... 7
1.2 Safety Symbols ......................................... 7
1.3 Nomenclature .......................................... 8
1.4 Specifications .......................................... 8
1.5 Disclaimer ............................................ 10
1.6 Safety Considerations ...................................... 10
1.7 Limited Warranty ........................................ 12
1.8 Transporting the ibidi Pump System ............................. 12
1.9 Repairing the ibidi Pump System ............................... 12
1.10 Waste Disposal – WEEE/RoHS Compliance Statement .................. 13
1.10.1 EU Directive WEEE ................................... 13
1.10.2 EU Directive RoHS ................................... 13
1.11 Regulatory Statement ...................................... 14
2 Intended Use of the ibidi Pump System 15
3 Equipment 16
3.1 Components of the ibidi Pump System ............................ 16
3.2 ibidi Pump ............................................ 17
3.3 Fluidic Unit ........................................... 18
3.3.1 The Valve Block ..................................... 20
3.3.2 The Pinch Valve ..................................... 20
3.4 Perfusion Sets .......................................... 21
3.5 ibidi µ-Slides ........................................... 22
3.6 Slide and Perfusion Set Selection Guide ........................... 25
3.7 Drying Bottle .......................................... 30
3.8 Computer with PumpControl Software ........................... 31
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4 Quick Start Guide 32
5 Basic Setup 33
5.1 General Setup of the Components .............................. 33
5.2 Connecting the Fluidic Unit to the Pump .......................... 34
5.3 Connecting the Drying Bottle ................................. 34
5.4 Installing the PumpControl Software ............................. 35
5.5 Connecting the ibidi Pump to the Computer ........................ 36
6 Setting Up an Experiment With Cells 37
6.1 Degassing of Slides, Tubing, and Medium .......................... 37
6.2 Mounting a Perfusion Set on the Fluidic Unit ........................ 38
6.3 Filling the Perfusion Set with Medium ............................ 41
6.4 Remove Air Bubbles from the Perfusion Set ......................... 42
6.5 Pinch Test ............................................ 43
6.6 Calibration of the Flow Rate .................................. 43
6.6.1 Connect the Calibration Slide to the Perfusion Set ................. 44
6.6.2 Flow Rate Measurement ................................ 45
6.6.3 Flow Calibration in the Software ........................... 47
6.7 Connect the Cell-Seeded Slide to the Perfusion Set ..................... 48
6.8 Start the Flow .......................................... 48
6.9 Observing Cells on the Microscope .............................. 48
6.10 Medium Exchange ....................................... 49
6.10.1 Medium exchange with left-over conditioned medium (option 1): ........ 49
6.10.2 Complete medium exchange (option 2): ....................... 49
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7 Installation of Special Setups 51
7.1 Working With Two or More Fluidic Units .......................... 51
7.1.1 Installation of Two or More Fluidic Units ...................... 51
7.1.2 Flow Calibration Of Two or More Fluidic Units .................. 52
7.2 Calibration of the Flow Rate with Several Slides in Serial Connection .......... 52
7.3 Oscillatory Flow Experiments ................................. 53
7.3.1 Setting up Oscillatory Flow with Two Fluidic Units ................ 53
7.3.2 Settings within the PumpControl Software ..................... 56
7.3.3 Oscillatory Experiment with Four Fluidic Units .................. 56
7.4 Pulsatile Flow Experiments .................................. 58
8 Technical Details 60
8.1 Working Principle of the ibidi Pump ............................. 60
8.2 Positive Versus Negative Air Pressure ............................ 62
8.3 Flow Characteristics ...................................... 64
8.4 Viscosity ............................................. 65
8.5 Shear Stress Calculations in ibidi Channel Slides ...................... 65
8.6 Working with Non-Implemented Flow Channels ...................... 68
9 Maintenance 71
9.1 Disinfection and Cleaning ................................... 71
9.2 Silica Beads from the Drying Bottle .............................. 71
9.3 Replacement Filters for Perfusion Sets ............................ 71
9.4 Fluidic Unit Filters ....................................... 71
9.5 Fluidic Unit Pinch Valves ................................... 71
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10 Troubleshooting 72
10.1 Air Bubbles ........................................... 72
10.1.1 Air Bubbles Emerging When Connecting the Slide ................. 72
10.1.2 Air Bubbles Emerging After a Few Hours ...................... 72
10.2 Cells are Detaching ....................................... 73
10.2.1 Cells Detach Before Starting the Flow ........................ 73
10.2.2 Cells Detach When Connecting the Slide to the Perfusion Set ........... 73
10.2.3 Cells Detach under Flow Conditions ......................... 74
10.3 Imbalanced Medium ...................................... 74
10.4 Medium Runs in One Direction Only ............................. 75
10.5 No Connection Between ibidi Pump and PumpControl Software ............. 76
10.6 Pressure Kickback After Pressure Switch Off ........................ 76
10.7 Flow Rate is Too Low or Absent ................................ 77
10.8 Flow Rate is too High ...................................... 77
10.9 Total Medium Volume in the Perfusion Set is Decreasing ................. 78
10.10Flow Direction in the Channel is Changing ......................... 78
10.11Pressure Lost Error ....................................... 78
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1 Preamble
1.1 Introduction
This manual is your guide to using the ibidi Pump System for flow experiments with the ibidi Chan-
nel Slides. It instructs first–time users how to use the instrument, and serves as a reference for expe-
rienced users.
Before using the ibidi Pump System, please read this instruction manual carefully, and make sure
that the contents are fully understood. This manual should be easily accessible to the operator at all
times during instrument operation. If this manual gets lost, order a replacement from ibidi.com.
To ensure operation safety, the ibidi Pump System must only be operated and maintained with the
supplied components, and according to the instruction manual.
The ibidi Pump System is for research use only! It is not intended for use in diagnostic procedures.
1.2 Safety Symbols
Note that the signal words WARNING,CAUTION and NOTE have specific meanings in this man-
ual. Do not proceed beyond a signal word until you have performed the indicated actions.
WARNING – A potentially hazardous situation which, if not avoided, could result
in serious injury or even death. Warning messages in the text are displayed in a gray
shaded box.
CAUTION – A potentially hazardous situation which, if not avoided, could result in
minor or moderate injury. It is also used to alert against damaging the equipment or
the instrument.
NOTE – Additional information to help achieve optimal instrument and assay per-
formance.
Symbols on the product identification label and back panel of the device:
CE Marking: This symbol indicates the product’s compliance with EU legislation.
This label is positioned on the back of the device and prompts you to read the manual
before using the device.
Product disposal: The symbol indicates that this product must be recycled/disposed
of separately from other household waste. See page 13 for details.
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1.3 Nomenclature
ibidi Pump Fluidic Unit
1.4 Specifications
Table 1: Specifications of the ibidi Pump System
Electrical Specifications Power Supply
Protection class I
Ingress protection rating IP 20
Overvoltage category II
External power supply AC 100–240 V, 50/60 Hz, 36–40 W
Input line voltage and current ibidi
Pump
max. DC 14–15 V, 2.4–2.85 A
Output voltage and current (to Flu-
idic Unit)
min. DC 14 V, 590 mA
Standby current 60 mA
Max. current 1000 mA
Max. current with 4 Fluidic Units 1475 mA
Operating Conditions of the ibidi Pump System
Operating area Enclosed rooms
Environmental operating tempera-
ture
15–40°C/59–104°F
Operating humidity ibidi Pump 80% RH up to 31°C/88°F, 30% RH up to 40°C/104°F
Operating humidity Fluidic Unit ≤100% (non-condensing)
Operating altitude max. 2000 m (atmospheric pressure 800–1060 hPa/11.6–15.4
psi)
Storage conditions -5–50°C/23–122°F, humidity <60% relative humidity (RH)
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Table 1: (continued)
Outer Dimensions and Characteristics of the Components
ibidi Pump 17 × 23 × 9 cm3(w×d×h)
3000 g/6.6 lbs
Fluidic Unit 8.5 × 13.5 × 27 cm3(w×d×h)
1100 g/2.4 lbs
Fluidic Unit Quad 26 × 18.5 × 24 cm3(w×d×h)
4420 g/9.7 lbs
USB cable 1.8 m
Power supply cable 2.0 m (power supply to wall)
1.2 m (power supply to device)
Electrical cable (FU to pump) 2.0 m
Air pressure tubing 2.0 m
Air pressure tubing drying bottle 2.1 and 0.6 m
Pressure Range of the ibidi Pump
Total pressure range 0-100 mbar
Recommended pressure range 5-95 mbar
Electrical Input Fluidic Unit
Switching current 110 mA (1 Fluidic Unit), 200 mA (2-4 Fluidic Units)
Hold current (state 2) 120 mA
Typical current @ 20 mbar 130 mA (state 1)/250 mA (state 2)
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1.5 Disclaimer
• ibidi shall not be held liable, either directly or indirectly, for any damage incurred as a result of
product use.
• The contents of this manual are subject to change without notice for product improvement.
• This manual is considered complete and accurate at publication.
• This manual does not guarantee the validity of any patent rights or other rights.
• If an ibidi software program doesn’t function properly, this may be caused by a conflict from
another program operating on the computer. In this case, take corrective action by uninstalling
the conflicting product(s).
• ibidi is a registered trademark of ibidi GmbH in Germany and other countries.
1.6 Safety Considerations
WARNING
• Only operate the ibidi Pump System with the supplied components.
• Only use the cables and plugs delivered with the system. The power plug of the control unit
must be inserted in an outlet with a ground (earth) contact.
• Do not replace detachable power cables by power cables with inadequate specifications. By
violating these instructions you risk electric shock and fire.
• Only use extension cables that have a protective ground wire.
• Do not operate the ibidi Pump System under conditions that pose a risk of explosion, implosion,
or the release of gases.
• Only operate the ibidi Pump System with water.
• Do not place flammable solids, liquids, gases, or gas outlets near the system (e.g., matches,
ethanol, propane gas, solvents). Do not bring these products in contact with any other compo-
nent of the system either.
• Do not operate a damaged ibidi Pump System. If the housing seems damaged or something is
rattling inside the controller, contact the ibidi service hotline for repair.
CAUTION
• Ensure that the external power supply is easily accessible. The ibidi Pump System must be
installed in a manner such that none of its components hinders access to the external power
supply.
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• Immediately replace damaged cords, plugs, or cables to avoid risk of personal injury or damage
to the instrument.
• Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser-
vice the ibidi Pump System.
• The external power supply should not be brought into contact with moisture. If the housing is
damaged, the external power supply should not be used.
• Avoid strong magnetic fields and sources of high frequency. The ibidi Pump System might not
function properly when located near a strong magnetic field or high frequency source.
• Avoid vibrations from vacuum pumps, centrifuges, electric motors, processing equipment, and
machine tools.
• Avoid dust and corrosive gas. Do not install the ibidi Pump System where it could be exposed
to high levels of dust or to outside air or ventilation outlets.
• Install the ibidi Pump System in a location that enables easy access for maintenance.
• Do not place heavy objects on the instrument.
• The weight of the ibidi Pump is approx. 3 kg. Moving the pump during operation will pose a
risk of personal injury or damage to the instrument.
• The ibidi Pump can build pressure up to 100 mbar. Do not unplug the fluidic connections dur-
ing pump operation. Pressurized liquid could emerge from the tubes and damage surrounding
equipment. Excess moisture can cause the external power supply or nearby electrical equip-
ment to short circuit.
• Do not suction any liquid into the ibidi Pump.
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1.7 Limited Warranty
Products manufactured by ibidi, unless otherwise specified, are warrantied for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects in the
product are found during this warranty period, ibidi will repair or replace the defective part(s) or
product free of charge.
This warranty does not apply to defects resulting from the following:
1. Improper or inadequate installation.
2. Improper or inadequate operation, maintenance, adjustment, or calibration.
3. Unauthorized modification or misuse.
4. Use of unauthorized tubing or fluidic connectors.
5. Use of consumables, disposables, and parts not supplied by an authorized ibidi distributor.
6. Corrosion due to the use of improper solvents, samples, or due to surrounding gases.
7. Accidents beyond ibidi’s control, including natural disasters.
This warranty does not cover consumables, such as cell culture chambers and dishes, tubes, fluidic
connectors, reagents etc.
The warranty for all parts supplied and repairs provided under this warranty expires on the warranty
expiration date of the original product.
1.8 Transporting the ibidi Pump System
The weight of the ibidi Pump is approx. 3 kg/6.6 lbs. The weight of the Fluidic Unit is approx. 1.1
kg/2.4 lbs. Moving the devices during operation will pose a risk of personal injury or damage to the
instrument.
For transport, switch off the ibidi Pump and then disconnect all cables and tubing from the controller
and peripheral components. Carry the devices carefully and avoid mechanical shocks.
1.9 Repairing the ibidi Pump System
For inquiries concerning repair service, contact the ibidi technical support and provide the model
name and serial number of your system.
ibidi GmbH
Service Hotline: [email protected]
CAUTION – Do not try to repair the ibidi Pump System by yourself. Disassembly of the
ibidi Pump System is not allowed. Disassembly poses a risk of personal injury or damage to the
devices. Contact ibidi technical support if there is a need to disassemble a device.
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1.10 Waste Disposal – WEEE/RoHS Compliance Statement
The European Union (EU) has enacted two directives, the first on product recycling (Waste Electrical
and Electronic Equipment, WEEE) and the second on limiting the use of certain substances (Restric-
tion on the use of Hazardous Substances, RoHS).
1.10.1 EU Directive WEEE
The ibidi Pump System must be disposed of in compliance with the WEEE Directive 2012/19/EC.
This symbol on the product is in accordance with the European Union’s Waste Electrical and Elec-
tronic Equipment (WEEE) Directive. The symbol indicates that this product must be recycled/dis-
posed of separately from other household waste. It is the end user’s responsibility to dispose of this
product by taking it to a designated WEEE collection facility for the proper collection and recycling of
the waste equipment. The separate collection and recycling of waste equipment will help to conserve
natural resources and protect human health and the environment. For more information about recy-
cling, please contact your local environmental office, an electrical/electronic waste disposal company
or distributor where you purchased the product.
1.10.2 EU Directive RoHS
RoHS conformity is declared in the EU-conformity in Section 1.11.
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1.11 Regulatory Statement
EG-Konformitätserklärung
EC Declaration of Conformity
Diese Erklärung bescheinigt die Übereinstimmung mit den genannten Richtlinien,
ist jedoch keine Zusicherung von Eigenschaften. Die Sicherheitshinweise der
Produktdokumentation sind zu beachten.
This declaration certifies the conformity to he specified directives but not includes
any warranted quality of the instrument. The safety documentation of the product shall
be considered in detail
Wir / We
ibidi GmbH
Lochhamer Schlag 11
D-82166 Gräfelfing
erklären hiermit die Übereinstimmung des genannten Produktes mit der Richtlinie 2014/35/EU - Niederspannungsrichtlinie
und mit der Richtlinie 2014/30/EU über die Elektromagnetische Verträglichkeit.
Bei Änderungen am Produkt, die nicht von uns autorisiert wurden, verliert diese Erklärung ihre Gültigkeit.
We declare the compliance of the product with the requirements of the Directive 2014/35/EU - Low Voltage Directive
and with the Directive 2014/30/EU about the Electromagnetic Compatibility.
Any modification to the product, not authorized by us, will invalidate this declaration.
Laborgerät / laboratory equipment:
ibidi Pump System ibiPump 2
Der oben beschriebene Gegenstand erfüllt die Vorschriften der Richtlinie 2011/65/EU vom 08. Juni 2011
zur Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro- und Elektronikgeräten.
The object of the declaration described above is in conformity with Directive 2011/65/EU of 8 June 2011
on the restriction of the use of certain hazardous substances in electrical and electronic equipment.
Das Produkt entspricht den unten aufgeführten Normen:
The product meets the requirements of the following standards:
Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und Laborgeräte - Teil 1: Allgemeine Anforderungen Safety
requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements
Elektrische Mess-, Steuer-, Regel und Laborgeräte. EMV-Anforderungen. Allgemeine Anforderungen
Electrical equipment for measurement, control and laboratory use. EMC requirements. General requirements
Das Produkt ist gekennzeichnet mit/ The product is marked with
Gräfelfing, den 27.06.2019 Dr. Valentin Kahl
Gräfelfing, 2019-06-27 Geschäftsführer
Ort/Datum Name, Funktion Unterschrift
Place/date Name, Function Signature
DIN EN 61010-1:2011
DIN EN 61326-1:2013
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ibidi Pump System
2 Intended Use of the ibidi Pump System
The ibidi Pump and Fluidic Unit(s) create unidirectional long-term flow of medium within a channel
slide (e.g., ibidi Channel Slides). This constant flow mimics physiological conditions for cell types,
like endothelial cells of the blood or lymphatic system, which experience constant flow in vivo.
The mechanical force generated by fluid flow on cells is called (wall) shear stress ( dyn
cm2or Pa). Under
physiological conditions, a laminar flow leads to a shear stress on the cell layer on the vessel wall
which is proportional to the velocity of the fluid. The shear stress varies among different tissues and
organisms.
The ibidi Pump System offers the following advantage for cell culture under flow:
• Covering of the whole physiological range of shear stresses.
• Precise control of flow conditions with the PumpControl software.
• Defined shear stress calculation in the ibidi Channel Slides.
• Unidirectional flow for long-term studies (up to weeks).
• Oscillatory and pulsatile flow to mimic turbulent flow situations and pulsatile blood flow.
• Easy access to the culture vessel for imaging on the microscope during incubation.
• Experimental setup under sterile conditions possible.
• Minimization of the medium consumption with a circulating medium flow.
• Minimization of mechanical stress on suspended cells to avoid destruction and non-specific
cell-activation.
The ibidi Pump System is for research use only! It is not intended for use in diagnostic procedures.
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3 Equipment
The ibidi Pump System consists of the ibidi Pump, the Fluidic Unit(s), and disposable parts, such as
Perfusion Sets and Slides.
3.1 Components of the ibidi Pump System
An overview of the different ibidi Pump System versions is given in this section. Table 2lists all
available options of the ibidi Pump System.
Table 2: Overview of the ibidi Pump System Variants
Cat. No. Product Name Description
10902 ibidi Pump System Complete ibidi Pump System with 1 Fluidic Unit, 1 sterile
Perfusion Set and all cables and components needed. The
details are listed below.
10906 ibidi Pump System Quad Complete ibidi Pump System with 1 Fluidic Unit Quad,
2 sterile Perfusion Sets and all cables and components
needed. The details are listed below.
10903 Fluidic Unit Switching valves for various flow assays, suitable for all
Perfusion Sets and channel µ-Slides
10904 Fluidic Unit Quad 4 Fluidic Units on a stable plate, switching valves for vari-
ous flow assays, suitable for all Perfusion Sets and channel
µ-Slides
The following parts are included in the ibidi Pump System (#10902 and #10906).
• ibidi Pump
• External Power Supply (country-specific) for the ibidi Pump
• USB cable to connect the pump to your PC
• USB flash drive with the latest PumpControl software
• 1 Fluidic Unit with Reservoir Holder (#10902)/1 Fluidic Unit Quad with Reservoir Holders
(#10906)
• Fluidic Unit cable(s) to connect Fluidic Unit(s) and Pump (length 2 m); 1 per Fluidic Unit
• Non-sterile Perfusion Set (1 per Fluidic Unit)
• Drying bottles filled with orange Silica beads (2)
• Connection cap for the drying bottle
• Air pressure tubing (2 m)
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• Air pressure splitter set to connect the Fluidic Unit Quad (only #10906)
• Short, yellow-marked air pressure tube (0.6 m); rigid air tube to connect the pump to the drying
bottle
• Long, black-marked air pressure tube (2.1 m); rigid air tube to connect the drying bottle to the
inside of the incubator
• Filter bubbler for the drying bottle
• Sterile replacement filter (1 per Fluidic Unit)
• Hose clip (1 per Fluidic Unit)
• Laptop with pre-installed PumpControl software
The following parts are provided, but are only needed for experiments using more than one Fluidic
Unit:
• Air Pressure Splitter Set for 2 Fluidic Units
• Air Pressure Splitter Set for 3 Fluidic Units
• Air Pressure Splitter Set for 4 Fluidic Units
• Oscillatory Flow Kit for 2 Fluidic Units
• Oscillatory Flow Kit for 4 Fluidic Units
The following parts must be ordered separately:
• Sterile Perfusion Sets
• Sterile µ-Slides Luer Type
3.2 ibidi Pump
The ibidi Pump can generate air pressure up to 100 mbar. The most precise working range is 5 to 95
mbar. Additionally, the pump can set the air flow direction. When using positive pressure, the pump
will expel air from the front port, and intake it from the rear port. When using negative pressure
the pump will take in air from the front port and expel it from the rear port. The use of positive or
negative pressure or air flow is detailed in Section 8.2. Optimal conditions are achieved using positive
pressure for experiments. In addition to the generation of air pressure, the ibidi Pump controls the
switching times of the Fluidic Unit(s). Up to four Fluidic Units can be controlled simultaneously with
one pump. Communication with the computer is achieved via a double shielded USB interface (see
also Section 10.11).
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Figure 1: ibidi Pump front side with air pressure front port and status LEDs.
Rear air inlet USB Power connector
Fluidic Unit
cable ports
Power
USB
1
2
3
4
ibidi Air Pressure Pump 2
Fluidic Unit control ports
Rear air inlet
14 V
1.7 - 3.0 A
Type:
Serial number:
Production date:
ibiPump 2
P0600
11/2018
ibidi GmbH
Am Klopferspitz 19
D-82152 Martinsried
Germany
[phone]
www.ibidi.com
Figure 2: Rear view of the ibidi Pump with rear air port, USB and power supply port, and Fluidic
Unit cable ports.
3.3 Fluidic Unit
The Fluidic Unit holds the Perfusion Set (fluidic reservoirs and tubing), and performs the switching
operations to generate the unidirectional constant flow in the flow chamber. The Fluidic Unit’s (Fig-
ure 3) active components are the two switching valves (V1) and (V2). There are two connectors in the
rear of the Fluidic Unit, one electrical connection for the valve control and another for the pressurized
air. Both connect the Fluidic Unit to the ibidi Pump.
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Figure 3: Front and back view of the Fluidic Unit.
The Fluidic Unit can be equipped with a choice of one of three holder sizes, for the respective syringe
reservoir sizes. The Reservoir Holder can easily be exchanged by the user. The standard reservoirs
are indicated in Table 5on page 22.
Table 3: Overview of Reservoir Holders and compatible Perfusion Sets and Filter/Reservoir Sets
Reservoir Holder Cat. No. Compatible Perfusion Sets Cat. No.
for Fluidic Unit, 10 ml
for Fluidic Unit Quad, 10 ml
10976
10986
Perfusion Set RED
Perfusion Set YELLOW/GREEN
Perfusion Set ORANGE
Perfusion Set BLUE
Perfusion Set WHITE
Perfusion Set GREY
Filter/Reservoir Set, 10 ml
10962
10964
10969
10961
10963
10968
10971
for Fluidic Unit, 2 ml
for Fluidic Unit Quad, 2 ml
10977
10987
Perfusion Set YELLOW
Perfusion Set BLACK
Perfusion Set BROWN
Filter/Reservoir Set, 2 ml
10965
10966
10967
10972
for Fluidic Unit, 50 ml 10978 Filter/Reservoir Set, 50 ml 10974
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Figure 4: 2 ml syringe reser-
voirs
Figure 5: 10 ml syringe reser-
voirs
Figure 6: 50 ml syringe reser-
voirs
Important!
To clean the Fluidic Unit, wipe the outside with an alcohol soaked paper towel. Never spray
disinfectant directly on the unit, which could damage the valve electronics.
3.3.1 The Valve Block
The valve block (V1) is located on top of the aluminum block of the Fluidic Unit. The function of the
valve is to guide the air pressure either to the right or left reservoir. The valve block is a magnetic
valve. Be careful not to draw any liquid into this valve!
3.3.2 The Pinch Valve
The pinch valve (V2) at the front of the Fluidic Unit squeezes the tubing that is inserted into its slots
with a movable bar, pinching off the inserted tubing parts and thus blocking fluid flow. The valve
has four slots (two forward and two rear slots), so that four pieces of tubing can be inserted at once.
Either the front or the rear tubing parts are closed at a time (two switching states).
Important!
Be aware that the pinch valve can wear out over time. The performance of the valve can be
checked as follows: Perform the pinch test and observe the actuation of the bar in the pinch valve.
If you see the bar moving inside the valve and the pinch test is ok, the valve works properly. If
not, contact ibidi or your local supplier for repair.
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