IBS MediaClave User manual

User Manual 3.4
Bedienungsanleitung 3.4
Mode d’emploi 3.4

© 2009 INTEGRA Biosciences AG, Switzerland
All rights reserved. This manual may not be copied or reproduced in whole or in part in any way without the prior express written permission
of INTEGRA Biosciences. The company reserves the right to make alterations without prior notice.
Notwithstanding the above statements, INTEGRA Biosciences accepts no liability for possible mistakes or their consequences arising from
this manual.
This manual is identified by Art.No. 135900
Declaration of conformity
INTEGRA Biosciences AG - CH-7000 Chur, Switzerland
declares on its own responsibility that the product,
in accordance with the EC directives
is in compliance with the following standards or normative documents:
Standards for Canada and USA
Description Type, model
MEDIACLAVE 135020, 135025, 135030, 135035
73/23/EEC Low voltage equipment
89/336/EEC Electromagnetic compatibility
97/23/EC Pressure equipment
2002/95/EC Restriction of Hazardous Substances
2002/96/EC Waste Electrical and Electronic Equip-
ment
EN 61010-1:2001-02 Safety requirements for electrical equip-
ment for measurement, control and lab-
oratory use - General requirements.
EN 61010-2-041:1998 Particular requirements for autoclaves
using steam for the treatment of medi-
cal materials and for laboratory proc-
esses.
EN 61326-1 +A1:1998+A2:2000 Electrical equipment for measurement,
control and laboratory use - EMC
requirements.
CAN/CSA-C22.2 No.1010.1-92 Safety requirements for electrical equip-
ment for measurement, control and lab-
oratory use - General requirements.
UL Std. No. 3101-1 Safety requirements for electrical equip-
ment for measurement, control and lab-
oratory use - General requirements.
FCC, Part 15, Class A Emission
Chur, Date
June 10, 2009 Elmar Morscher, CEO Thomas Neher, Quality Manager
This equipment has been
tested and found to com-
ply with the limits for a
Class A digital device,
pursuant to both Part 15
of the FCC Rules and the
radio interference regula-
tions of the Canadian De-
partment of
Communications.
Theselimitsaredesigned
to provide reasonable
protection against harm-
ful interference when the
equipment is operated in
a commercial environ-
ment.
This equipment gener-
ates,usesandcanradiate
radio frequency energy
and, if not installed and
used in accordance with
the instructions manual,
may cause harmful inter-
ference to radio commu-
nications.

User Manual 3.4
INTEGRA Biosciences PAGE I
ENGLISH
Table of contents
1 MediaClave overview ................................................................................................ 1
1.1 About the MediaClave ......................................................................................................1
1.1.1 Introducing the MediaClave ...................................................................................... 1
1.1.2 Intended use ............................................................................................................. 1
1.1.3 Basic concepts ......................................................................................................... 2
1.2 Using the manual ............................................................................................................. 2
1.2.1 About the manual ..................................................................................................... 2
1.2.2 User manual symbols ............................................................................................... 3
1.3 General information ......................................................................................................... 4
1.3.1 Safety precautions .................................................................................................... 4
1.3.2 Safety regulations concerning service and maintenance ......................................... 5
1.3.3 Equipment disposal................................................................................................... 5
1.3.4 Warranty and liability ................................................................................................ 5
2 Installation ................................................................................................................. 7
2.1 Introduction ...................................................................................................................... 7
2.2 Operating environment .................................................................................................... 7
2.3 MediaClave drawings .......................................................................................................8
2.3.1 MediaClave vessel overview .................................................................................... 8
2.3.2 MediaClave rear panel ............................................................................................. 9
2.4 Electricity and water ....................................................................................................... 10
2.4.1 Provision of electricity ............................................................................................. 10
2.4.2 Water supply .......................................................................................................... 10
2.5 Filter and tubing installation ........................................................................................... 11
2.5.1 Sterile filter installation ........................................................................................... 11
2.6 Moving the MediaClave ................................................................................................. 11
2.6.1 Moving procedure ................................................................................................... 11
3 Operating controls .................................................................................................. 13
3.1 Introduction .................................................................................................................... 13
3.2 Preparation for different operational modes ................................................................... 13
3.2.1 Mode MEDIACLAVE .............................................................................................. 13
3.2.2 Mode AUTOCLAVE ................................................................................................ 15
3.2.3 Mode WATERBATH ............................................................................................... 17
3.3 Switching On and Off the MediaClave ........................................................................... 17
3.3.1 MediaClave start display ........................................................................................ 17
3.3.2 Soft key menu options explained ........................................................................... 18
3.3.3 LANGUAGE menu ................................................................................................. 19
3.3.4 PARAMETER menu ............................................................................................... 20

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INTEGRA Biosciences
PAGE II
4 Instruction for use .................................................................................................. 25
4.1 Introduction .................................................................................................................... 25
4.2 Safety precautions and specifications ............................................................................ 25
4.2.1 MEDIACLAVE and AUTOCLAVE sterilisation ....................................................... 25
4.2.2 WATERBATH ......................................................................................................... 25
4.3 Program operation .........................................................................................................26
4.4 MEDIACLAVE program operation ................................................................................. 26
4.4.1 AUTOCLAVE program operation ........................................................................... 30
4.4.2 WATERBATH program operation .......................................................................... 34
5 Cleaning instructions ............................................................................................. 37
5.1 Introduction .................................................................................................................... 37
5.2 Manually controlled cleaning routines ............................................................................ 37
5.2.1 General cleaning routine ........................................................................................ 37
5.2.2 Removing residual dirt ............................................................................................ 38
5.3 MediaClave Cleaning menu ........................................................................................... 39
5.3.1 Cleaning procedure ................................................................................................ 39
5.3.2 Overview ................................................................................................................39
5.3.3 EMPTY menu ......................................................................................................... 39
5.3.4 CLEAN menu ......................................................................................................... 40
6 Maintenance ............................................................................................................ 43
6.1 Introduction .................................................................................................................... 43
6.2 Paper handling ............................................................................................................... 43
6.2.1 Replacing the Printer paper roll .............................................................................. 43
6.3 Temperature calibration ................................................................................................. 45
6.3.1 Overview ................................................................................................................45
6.3.2 When to perform calibration ................................................................................... 45
6.3.3 Temperature calibration routine ............................................................................. 45
6.4 Troubleshooting + Error handling ................................................................................... 46
6.4.1 Possible solutions for problems .............................................................................. 46
6.4.2 Error handling ......................................................................................................... 47
6.5 Frequently Asked Questions .......................................................................................... 49
7 Accessories and Technical specifications ........................................................... 51
7.1 Introduction .................................................................................................................... 51
7.2 MediaClave accessories ................................................................................................ 51
7.2.1 MediaClave accessories list ................................................................................... 51
7.3 Country versions ............................................................................................................ 52
7.3.1 Electrical ratings ..................................................................................................... 52
7.4 Software default values .................................................................................................. 52
7.4.1 Overview ................................................................................................................52

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7.4.2 MEDIACLAVE programs 1–13 (1–9 L of medium) ................................................. 52
7.4.3 MEDIACLAVE program 14 (Without pressure test) ............................................... 53
7.4.4 MEDIACLAVE program 15 (Blood Agar) ................................................................ 53
7.4.5 General default values ........................................................................................... 54
7.5 Limitations and general notes ........................................................................................ 54
7.5.1 Overview ................................................................................................................54
7.5.2 Limit values within mode MEDIACLAVE ................................................................ 55
7.5.3 Limit values within mode AUTOCLAVE ................................................................. 55
7.5.4 Limit values within mode WATERBATH ................................................................. 55
7.6 Input / Output connectors ............................................................................................... 56
7.6.1 Overview ................................................................................................................56
7.6.2 First serial port RS232 ............................................................................................ 56
7.6.3 Second serial port RS232 ....................................................................................... 57
7.6.4 Interface for external safety cover lock switch and alarm ....................................... 58
7.7 Progress printouts .......................................................................................................... 59
7.7.1 Overview ................................................................................................................59
7.7.2 Test print ................................................................................................................ 59
7.7.3 Progress print ......................................................................................................... 60
7.8 MediaClave physical properties .................................................................................... 61
7.8.1 MediaClave data .................................................................................................... 61
7.8.2 Installation data ...................................................................................................... 61
8 Manufacturer and Customer Service .................................................................... 63
8.1 Manufacturer: ................................................................................................................. 63
8.2 Customer Service: .........................................................................................................63
9 Glossary ................................................................................................................... 65

Table of contents
INTEGRA Biosciences
PAGE IV

User Manual 3.4
INTEGRA Biosciences Page 1
ENGLISH
1 MediaClave overview
1.1 About the MediaClave
1.1.1 Introducing the MediaClave
Figure 1.1: The MediaClave
1.1.2 Intended use
The MediaClave is a highly versatile piece of equipment. It operates as a stand-alone device used to pro-
gram and control three integrated operation modes: MEDIACLAVE, AUTOCLAVE and WATERBATH.
The MEDIACLAVE program is used to prepare culture media. Insertion of a stainless steel base plate and
flexible PT100 temperature probe converts the MediaClave into the AUTOCLAVE. The AUTOCLAVE pro-
gram is used as a bench-top autoclave for sterilisation of culture media in single containers. The WATER
BATH program is used as a conventional water bath.
NOTE: Consult section 7.2.1 for a list of all standard and optional MediaClave accessories.
Safety Cover Lock
Safety Valve
Vessel Lid
Safety Cover
Outlet Cap
Inlet Cap
Form Feed Button
Printer Manometer Magnetic Front Panel
Graphic User
Interface (GUI)

MediaClave overview
INTEGRA Biosciences
Page 2
1.1.3 Basic concepts
The MediaClave offers many features to the user:
• Automatic initialisation and self-test execution
• Mode MEDIACLAVE – culture preparation of 1 to 9 L of agar containing medium or nutrient liquid
• Mode AUTOCLAVE – autoclave for sterilisation of culture media in single containers.
• Mode WATERBATH – fully running thermostatic water bath
• Preparation of highly sensitive culture media in a gentle and reproducible fashion
• Easy addition of growth additives, inhibitors and supplements
• Highly efficient intra-cooler – cool-down phase 60% faster than conventional jacket cooling; 60% less
water consumption
• Easy to follow, user-friendly menu operation via four soft keys
• Low energy costs
• Up to 15(x3) individual programs can be stored
• Alarmed error messages
• 4-level security system regarding temperature and pressure control. Over pressure valve and over-
heating sensor prevent danger to people and appliance.
• Full traceability of individual instrument functions and programmed processes – progress printouts
via the integrated printer
• Adjustment of the graphic user interface (GUI) to suit the laboratory environment
• Easy maintenance
• Space-saving compact design
• Host PC connection
• Interface for external serial printer (from software version 1.9.1 and with additional cable installed
1.2 Using the manual
1.2.1 About the manual
The MediaClave User manual has been designed to allow general users access to required information.
Any departure from the procedures outlined in theMediaClaveUsermanualmay leadto erroneous results
or instrument malfunctions and may adversely affect the safety of the MediaClave.
ATTENTION:The MediaClave is not suitable for sterilisation of medical devices.
NOTE:The MediaClave functionality, described in User manual version 3.4, is based on Media-
Clave software version 1.9.1 and higher. The interface for an external printer is only usable, if a
special cable is installed into MediaClave additinally to the software 1.9.1. (cable order number:
135245). MediaClave instruments with serial number 13505601 and later have this cable installed
by default.

User Manual 3.4
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The MediaClave User Manual is divided into 9 parts
1.2.2 User manual symbols
The signal words WARNING, ATTENTION and NOTE are included in this manual to emphasise im-
portant instructions and alert the user to potential hazards. They have a special hazard alert symbol.
Chapter Name Description
1MediaClave overview General information
2Installation Installation, moving
3Operating controls MediaClave preparation
4Instructions for use MEDIACLAVE, AUTOCLAVE, WATERBATH operation
5Cleaning instructions Cleaning procedures
6Maintenance Error- and Troubleshooting, Calibration, Paper-handling
7Accessories and Technical
specifications Accessories, default values, ports, physical properties
8Manufacturer and Customer
service Addresses
9Glossary Term definitions
Table 1.1: User manual overview
Symbol Definition
WARNING indicates a potentially hazardous situation that, if not avoided, could
result in damage to the instrument or personal injury. This signal word is used
only in extreme situations and, therefore, requires special attention.
ATTENTION indicates special problems or important information. Read the
accompanying text carefully as it is important for understanding the specific topic
or command.
NOTE indicates information that is useful, but not essential, to a task. Read the
accompanying text carefully as it can help to clarify particular issues.
Table 1.2: Legend of symbols

MediaClave overview
INTEGRA Biosciences
Page 4
1.3 General information
1.3.1 Safety precautions
1.3.1.1 General safety precautions
• The MediaClave User manual must be read in detail before operating the MediaClave
• In the event of a situation arising where safe operation of the MediaClave cannot be guaranteed, the
instrument must be switched off and locked until qualified personnel can repair it. Such a situation
may include one of the following scenarios:
• The MediaClave is visibly damaged
• The MediaClave stops working properly
• The mains power switch automatically switches to position 0
• Steam escapes through the lid
• The safety cover or vessel lid does not close
• Repairs must only be carried out by authorised personnel, using genuine spare parts. In particular,
only qualified personnel can connect and replace the mains plug.
• Theappliance must onlybe operated by appropriately trained andinstructed persons who are familiar
with all aspects of safety relating to this appliance.
• Apart from the safety notes in these instructions, the specific requirements for the prevention of ac-
cidents as well as the generally applicable rules for work place safety must also be observed.
• These installation and operating instructions must at all times be readily available where the Media-
Clave is used.
• The MediaClave must not be used for any purpose apart from the outlined applications.
• Do not open the MediaClave. Do not carry out any conversions and alterations on the device.
1.3.1.2 Precautions during operation
• Do not leave the MediaClave unattended when running a program.
• Do not open the vessel lid when pressure is greater than 0.1 bar and temperature exceeds 80°C.
• Do not spill liquids on the MediaClave. If the MediaClave requires cleaning, consult chapter 5.
ATTENTION:The MediaClave is subject to high thermal and compressive stress during the sterilisation
cycle. It is therefore imperative to observe the safety notes of this User manual.
WARNING:For personal security, the MediaClave must be switched off and disconnected from power
supply before any installation, repair or maintenance work is carried out.
WARNING:To prevent unintended damage to the appliance it is absolutely essential that you read and
understand the operatinginstructions. Furthermore, the owner is responsible for ensuring that appropriate
instructions are provided to users of the appliance.
WARNING:First carefully read these instructions before operating the MediaClave.
WARNING: No water must flow over the front panel!