ISCO GLS User manual

GLS
Sampler
This pocket guide is not intended to replace
the instruction manual provided on CD. Read
the Glacier Manual on CD thoroughly before
operating the sampler.
COPYRIGHT © 2002 by
Isco, Inc., 4700 Superior St., Lincoln,
Nebraska, U.S.A. 68504
Phone: (402) 464-0231
Toll Free: (800) 228-4373
FAX: (402) 465-3022
Part #69-2953-030
Revision A, March, 2003


iii
GLS
Sampler
Table of Contents
1. Installation
1.1 Introduction . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Sampler Preparation Checklist . . . . . 1-2
1.3 Inspecting the Pump Tube . . . . . . . . 1-3
1.4 Discharge Tube . . . . . . . . . . . . . . . . . 1-4
1.5 Installing a Bottle . . . . . . . . . . . . . . . 1-5
1.6 Sample Cooling . . . . . . . . . . . . . . . . . 1-5
1.7 Installing a Power Source . . . . . . . . . 1-6
1.7.1 To install an Isco Model 900
power source: . . . . . . . . . . . . . 1-6
1.8 Suction Line and Strainer . . . . . . . . . 1-7
1.8.1 Cutting the Suction Line . . . . 1-8
1.8.2 Attaching the Strainer . . . . . . 1-8
1.8.3 Connecting the Suction Line . 1-9
1.9 Connecting External Devices . . . . . 1-10
1.10 Positioning the GLS . . . . . . . . . . . . . 1-11
1.10.1 Positioning the Strainer and
Suction Line . . . . . . . . . . . . . 1-12
1.11 Locking the GLS . . . . . . . . . . . . . . . 1-14
2. Programming
2.1 The Keypad . . . . . . . . . . . . . . . . . . . . 2-1
2.2 Programming the GLS . . . . . . . . . . . 2-2
2.3 One-button Programming . . . . . . . . . 2-3
2.3.1 Stored Program . . . . . . . . . . . . 2-3
2.4 Standard Programming . . . . . . . . . . . 2-4
2.4.1 Sample Pacing . . . . . . . . . . . . 2-5
2.4.2 Pacing Interval . . . . . . . . . . . . 2-6

GLS Sampler
iv
2.4.3 Bottle Volume . . . . . . . . . . . . . 2-7
2.4.4 Number of Samples . . . . . . . . 2-8
2.4.5 Sample Volume . . . . . . . . . . . . 2-9
2.4.6 Program Start Time . . . . . . . 2-10
2.4.7 Suction Line . . . . . . . . . . . . . 2-13
2.5 Storing a Program . . . . . . . . . . . . . . 2-14
2.6 Setting the Time and Date . . . . . . . 2-14
2.7 Calibration . . . . . . . . . . . . . . . . . . . . 2-15
3. Operation
3.1 Starting a Program . . . . . . . . . . . . . . 3-1
3.1.1 Program Start Times . . . . . . . 3-2
3.1.2 Sampler Inhibit . . . . . . . . . . . 3-2
3.2 Run State Displays . . . . . . . . . . . . . . 3-4
3.2.1 Collecting a sample . . . . . . . . 3-4
3.2.2 Waiting to sample . . . . . . . . . . 3-4
3.2.3 Errors . . . . . . . . . . . . . . . . . . . 3-5
3.2.4 Pausing or Stopping a
Program . . . . . . . . . . . . . . . . . . 3-5
3.3 Program Completion . . . . . . . . . . . . . 3-6
3.4 Post-sampling Activities . . . . . . . . . . 3-6
3.4.1 Recovering the Sampler . . . . . 3-7
3.4.2 Preparing the Full Sample
Bottle . . . . . . . . . . . . . . . . . . . . 3-7
3.4.3 Viewing the Log . . . . . . . . . . . 3-8
3.5 Errors . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.6 Grab Samples . . . . . . . . . . . . . . . . . . 3-11
3.7 Replacing the Pump Tube . . . . . . . . 3-12

GLS Sampler
1-2
1.2 Sampler Preparation
Checklist
The following checklist can be used as a guide
to prepare the GLS for each use.
1. Inspect the pump tube.
See Section 1.3.
2. Check the discharge tube.
See Section 1.4.
3. Install the bottle.
See Section 1.5.
4. Icing the bottle (optional).
See Section 1.6.
5. Connect a power source.
See Section 1.7.
6. Connect a suction line and
strainer. See Section 1.8.
7. Connect external devices
(essential for flow-paced
sampling or sampler
inhibiting). See Section 1.9.
8. Place the GLS in position. See
Section 1.10.
9. Calibrate sample volumes
(optional). See Section 2.7.
10. Lock the GLS (optional). See
Section 1.11.

Section 1 Installation
1-3
1.3 Inspecting the Pump Tube
Inspect the pump tube before running a
program. If the pump tube fails, the GLS will
not be able to collect samples.
WARNING
Moving parts can cause injuries. Remove power
before inspecting pump tube.
To inspect the pump tube:
1. Disconnect the power from the 12V Input
Power connector.
2. Remove the pump housing cover by
loosening the four thumbscrews.
3. Visually inspect the pump tube for cracks
where it is compressed by the rollers. If the
tube is cracked, it must be replaced. See
section 3.7.
4. Visually inspect the inside of the pump
housing. The housing and rollers should be
free from debris.
5. Replace the pump housing cover and
tighten the thumbscrews.
Note
The GLS will display a pump tube warning as part of the
View Log screens when it exceeds 500,000 pump
counts.

GLS Sampler
1-4
Note
The GLS automatically resets the pump tube count to
500,000 after it displays the warning message.
Note
Isco pump tubes are made from medical-grade
Silastic™ tubing. This tubing will not contribute any
organic material to the samples.
1.4 Discharge Tube
The discharge tube is located inside the center
section. It is a 3⁄8-inch (9 mm) I.D., 81⁄4-inch (210
mm) long piece of medical-grade Silastic™
tubing.
The discharge tube should be well fitted over
the bulkhead fitting and routed through the
tube guide. The tube must be free of twists or
kinks. The tube should extend about 11⁄2inches
(38 mm) past the end of the tube guide (even
with the bottom of the center section). This
length of tubing is necessary for the GLS to
detect an overfilled bottle.

Section 1 Installation
1-5
1.5 Installing a Bottle
The base section of the GLS is designed to hold
five different types of bottles:
• 2.5 gallon (10 liter) Polyethylene
• 2.5 gallon (10 liter) Glass
• 1 gallon (3.8 liter) Polyethylene (with
bottle deck)
• 1 gallon (3.8 liter) Glass (with bottle
deck)
• 2 gallon (7.6 liter) ProPak Liner
To install the bottle:
2.5 and 2 gallon (10 and 7.6 liter) - place the
bottle in the GLS base section.
1 gallon (3.8 liter) - place the bottle deck into
the GLS base section. Set the bottle on top of
the bottle deck.
1.6 Sample Cooling
You can cool the samples by placing crushed ice
around the outside of the bottle. When using a 2
or 2.5 gallon (7.6 or 10 liter) bottle, the base
section can hold 10 pounds (4.5 kg) of ice. When
a bottle deck and 1 gallon (3.8 liter) bottle is
installed, the base section can hold 14 pounds
(6.3 kg) of ice. Frozen gel packs are sometimes a
convenient alternative to ice. For maximum
cooling, fill the base (with the bottle and deck in
place) with water and freeze the base section
and its contents.

GLS Sampler
1-6
1.7 Installing a Power Source
The GLS must be powered by a 12-Volt DC
power source. Isco recommends using one of the
following Model 900 series power sources:
•Batteries-
· Model 934 Nickel Cadmium Battery,
4.0Ah
· Model 946 Lead-Acid Battery, 6.5Ah
• Power Packs -
· Model 913 High Capacity Power Pack,
120 Volts AC
· Model 923 High Capacity Power Pack,
240 Volts AC
· Model 914 Battery Backed Power Pack,
120 Volts AC
· Model 924 Battery Backed Power Pack,
240 Volts AC
1.7.1 To install an Isco Model 900 power
source:
1. Place the power source into the center
section recess.
2. Secure the power source by pulling the
elastic bands over it and attach the clip to
the two posts. Note that there are two sets
of holes - use the set that places the clip
against the power source.
3. Attach the two-pin connector to the Input
Power connector on the back of the
controller.

Section 1 Installation
1-7
4. Power Packs Only - Route the AC line cord
though the center section’s Tubing/Cable
port.
Note
If you use a battery to power the GLS, always install a
fully charged one before running a program.
Note
Refer to Isco’s Power Products Guide, P/N
60-9003-092, for a complete description of each power
source.
1.8 Suction Line and Strainer
The suction line carries the liquid from the
sampling point to the GLS pump tubing. The
GLS is designed to use:
•1⁄4-inch (6 mm) I.D. vinyl tubing
•3⁄8-inch (9 mm) I.D. vinyl tubing
•3⁄8-inch (9 mm) I.D. Teflon® tubing with a
protective polyethylene jacket
The strainer reduces the possibility of debris
plugging the suction line. Types available are:
• CPVC body (3⁄8-inch I.D. only)
• Stainless Steel (1⁄4- and 3⁄8-inch I.D.)
Your application will dictate the best
combination of suction line and strainer.
To prepare the suction line and strainer:
1. Cut the suction line to the shortest feasible
length.

GLS Sampler
1-8
2. Attach a strainer to the suction line (Section
1.8.1).
3. Connect the suction line to the pump tube
(Section 1.8.2).
Note
The vinyl suction line does contain a very low ppm
(parts per million) level of phenols. If this affects your
samples, use the Teflon suction line.
1.8.1 Cutting the Suction Line
The suction line should be cut to the shortest
feasible length. This reduces the possibility of
cross-contamination between sample volumes
and extends the battery life. The suction line
can be easily cut with a knife.
When cutting the suction line, keep in mind the
length must be cut to the nearest whole foot or
decimeter. The length is measured from end to
end, without the strainer or tubing coupler.
If you have altered the length, press the
Calibrate button, and enter the new suction
line dimensions.
1.8.2 Attaching the Strainer
Items required:
Strainer Suction line
To attach the strainer to the suction line:
1. Heat the end of the suction line to make it
more pliable.
2. Screw the threaded end of the strainer into
the suction line.

Section 1 Installation
1-9
1.8.3 Connecting the Suction Line
Vinyl Suction Line
To connect the 1⁄4-inch (6 mm) or 3⁄8-inch (9 mm)
vinyl suction line to the pump tube:
Items required:
Suction line with strainer attached
Tubing coupler
1. Insert the end of the tubing coupler with the
black clamp into the upper pump tube.
2. Position the black clamp around the pump
tube and squeeze the sides of the clamp
together.
3. Push the vinyl suction line onto the end of
the tubing coupler with the white clamp.
4. Position the white clamp around the suction
line and squeeze the ends together.
Teflon Suction Line
To connect the Teflon suction line to the pump
tube:
Items required:
Suction line with strainer attached
3⁄4-inch (19 mm) diameter hose clamp
Tool to tighten clamp
1. Place a hose clamp on the upper pump tube.
2. Insert about 1 inch (25 mm) of the Teflon
suction line into the upper pump tube.
3. Position the clamp over the joined area and
tighten it.

GLS Sampler
1-10
1.9 Connecting External Devices
The GLS can be used with external devices that
control the sampler pacing, sampler inhibiting,
or both. The sampler pacing input can control
the rate of sample collection so that it is
proportional to the flow rate of a channel. This
input must be used when the Flow Paced
program option is selected (see Sample Pacing,
section 2.4.1). The sampler inhibit input can
delay the GLS operation until a monitored
parameter meets user-defined conditions.
These devices connect to the 6-pin Flow Meter
Connector located on the back of the GLS
controller. Compatible Isco devices include:
• Pacing and Inhibiting devices:
· 4100 Series Flow Loggers
· 4200 Series Flow Meters
· 2100 Series Flow Meters
• Pacing devices (non-Isco device
interfaces):
· 4-20 mA Input Interface
· Pulse Duration Input Interface
• Inhibiting devices:
· Liquid Level Actuator
Note
Flow pacing input signal (pin C) requirements - a 5 to 15
volt DC pulse or isolated contact closure of at least 25
milliseconds in duration.

Section 1 Installation
1-11
Note
Sampler inhibit signal (pin F) requirements - a low
(grounded) level of at least 5 seconds inhibits the
operation. A high (or open) level of at least 5 seconds in
duration restores the operation.
1.10 Positioning the GLS
There are a few considerations when selecting a
site for the GLS. The foremost concern should
be personal safety.
WARNING
The installation and use of this product may
subject you to hazardous working conditions that
can cause you serious or fatal injuries. Take any
necessary precautions before entering the
worksite. Install and operate this product in
accordance with all applicable safety and health
regulations, and local ordinances.
The following points should also be considered:
•Level surface - The GLS should be placed
on a level surface to prevent tipping or
spills.
•Support - The surface or mounting
method must be able to support the GLS
at full capacity. A GLS with battery, full
sample bottle, and ice may weigh as
much as 63 pounds (28.6 kg).
•Environmental - The GLS is designed for
use in harsh environments. However, you
should avoid installing the GLS in

GLS Sampler
1-12
locations where its components are
subject to chemical attack. Also,
prolonged exposure to direct sunlight will
eventually damage the ABS exterior.
•Avoid submersion - Although its
controller will resist damage (rated
NEMA 4x, 6), the GLS cannot prevent
the liquid from entering the base and
center sections. Liquid entering the base
section while the GLS is submerged will
most likely invalidate the collected
samples.
•Accessibility - The GLS must be installed
in a location where it can be recovered
easily without tipping or difficult
maneuvering.
•Security - The location may need to
provide some degree of security to
prevent tampering or vandalism.
The GLS can be installed in a manhole using
the optional suspension harness (P/N
60-2954-033). Contact your sales
representative or Isco for more information.
1.10.1 Positioning the Strainer and Suction
Line
After the GLS is in place, the strainer and
suction line should be properly positioned.
The strainer should be placed in the stream so
that representative samples are collected. The
intake should be in the main flow, not in an

Section 1 Installation
1-13
eddy or at the edge of flow. Its depth in the
stream can also be important. An intake placed
at the bottom of the stream may result in
excess heavy solids, while placement at the top
may result in the opposite.
The suction line should always be cut to the
shortest possible length. Route the suction line
so that it runs continuously downhill. Loops of
coiled suction line or low areas where the liquid
can pool will hold residual amounts of liquid
that will cross-contaminate sample volumes.
The suction line will tend to float when
sampling from deep flow streams. Refer to
Table 1-1. If the depths listed in the chart are
exceeded, anchor the line securely so that the
suction line and strainer do not become
dislodged.

GLS Sampler
1-14
1.11 Locking the GLS
Access to the inside of the GLS can be secured
by placing a padlock on the carrying handle.
Because the carrying handle must be
repositioned before gaining access, locking the
handle in an upright position secures the top
cover, center section and controller, and the
base section.
Table 1-1 Safe limits for unanchored
suction lines a
Strainer
Vinyl
1/4-inch
(6 mm)
Vinyl
3/8-inch
(9 mm)
Teflon
3/8-inch
(9 mm)
Stainless
Steel —22 feet
(6.7 m)
15 feet
(4.5 m)
CPVC — 4 feet
(1.2 m) —
a.Anchor the line and strainer when depth is
exceeded.

2-1
GLS
Sampler
Section 2 Programming
2.1 The Keypad
On/Off Button – When in the Off state
the On/Off button turns the GLS on and
places it in the Standby state. In any
other state, the On/Off button will place
the sampler in the Off state.
Number Entry Buttons – The
number-entry buttons allow you to enter
numerical values when prompted by an
interactive screen.
Arrow Button – The Arrow button
changes the current selection in an
interactive screen. Pressing the Arrow
button causes a different option to blink.
Enter Button – In an interactive state,
the Enter button accepts the selected
(blinking) option. In non-interactive
states, pressing the Enter button will
scroll through any additional displays.

GLS Sampler
2-2
2.2 Programming the GLS
There are two ways to program the GLS:
• One-button Programming
• Standard Programming
Stop Button – The Stop button
interrupts the current task and the
display reverts to the previous screen or
state. If you press the Stop key while a
number-entry screen is displayed, the
GLS restores the previous value.
Pressing the Stop key while running a
program places the GLS in the Paused
state.
Calibrate Button – Pressing the
Calibrate button while in the Standby
state takes you to the Calibrate Sample
Volume sequence of the programming.
Grab Sample Button – The Grab
Sample Button allows you to collect a
grab sample outside of the programmed
number of samples.
Program Button – The Program button
is used for the GLS One-button
Programming (see One-button
Programming, section 2.3).
Go Button – Pressing the Go button
places the GLS in the Run state using
the current program.
Table of contents