Jasco V-530 User manual

P/N:0302-0304C January 2003
V-530/550/560/570
Spectrophotometer
Instruction Manual
V -500 for W indow s
Ò

i
Safety Considerations
To ensure operation safety, this instrument must be operated correctly and maintained
according to a regular schedule. Carefully read to fully understand all safety precautions in this
manual before operating the instrument. Please take a moment to understand what the signal
words WARNING!, CAUTION, and Note mean in this manual.
(1) Safety symbols
Instruction manual symbol. If the product is marked with this symbol, refer to
the instrument manuals to protect the instrument against damage.
WARNING A WARNING indicates an potentially hazardous situation which, if not avoided,
could result in death or serious injury
.
CAUTION A CAUTION indicates a potentially hazardous situation which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against
damaging the equipment.
Do not proceed beyond a WARNING or CAUTION notice until you understand
the hazardous conditions and have taken the appropriate steps.
Note A Note provides additional information to aid the operator in obtaining optimal
instrument performance.

ii
(2) Warning Label
Warning labels are attached at several locations on this instrument. Do not remove,
deface or damage the warning labels. If a warning label peels off the instrument or
becomes illegible, contact your local JASCO distributor and state the part number of
the label you want to replace.
1) Warning for FUSE (Fig.1 or Fig. 2)
Part No.: 0822-0120A
2) Warning for GROUND (Fig.1 or Fig.2)
Part No.: 0822-0125A
Warning for FUSE Warning for GROUND
Figure 1 Warning Labels on the V-530 Back Panel
!WARNING
FOR CONTINUED PROTECTION
AGAINST RISK OF FIRE, REPLACE
ONLY WITH FUSE OF THE SPECIFIED
TYPE AND CURRENT RATINGS.
Only use fuses of the specified rating to protect
both operator and instrument from fire and other
hazards. When replacing a fuse, refer to the
hardware manual (4.4.1 Replacing the fuse). The
warning labels that pertain to fuse ratings are
located on the back panel of the instrument.
!WARNING
MAINS PLUG GROUND PIN
OR GROUND TERMINAL
MUST BE CONNECTED
TO GROUND.
This instrument must be grounded correctly;
either the mains plug ground pin or through the
ground terminal on the rear panel of the
instrument.

iii
Warning for FUSEWarning for GROUND
Figure 2 Warning Labels on the V-550/560/570 Back Panel
(2) Warning for carrying
The weight (in kilograms) of each instrument is as follows:
V-530 V-550 V-560 V-570
16.0 30.5 32.0 33.0
When moving the instrument, hold the handgrips at the bottom of the instrument
firmly (see Fig. 3).
Handgrips
Figure 3 Example: V-550/560/570 side view

iv
Regulatory Statements
CE Notice
Marking by the symbol indicates compliance of this JASCO system to the EMC
(Electromagnetic Compatibility) and Low Voltage Directives of the European Community. This
symbol indicates that this JASCO system meets the relevant basic safety and health
requirements of the EC Directive based on the following technical standards:
· EN55011 ---- ”Limits and Methods of Measurement of Radio Interference Characteristics of
Information Technology Equipment.” ---- Group 1, Class A.
WARNING: This is a Class A product. In a domestic environment this product may cause
radio interference, in which case the user may be required to take adequate
measures.
· EN50082-1 -- "Electromagnetic compatibility -- Generic immunity standard Part 1:
Residential, commercial, and light industry."
· IEC61000-4-2 -- "Electromagnetic compatibility for industrial-process measurement and
control equipment Part 2: Electrostatic discharge requirements."-- Severity level 3.
· IEC61000-4-3 -- "Electromagnetic compatibility for industrial-process measurement and
control equipment Part 3: Radiated electromagnetic field requirements." -- Severity level 2.
· IEC61000-4-4 -- "Electromagnetic compatibility for industrial-process measurement and
control equipment Part 4: Electrical fast transient/burst requirements."-- Severity level 3.
· IEC1010-1: 1990 + Amd.1: 1992 + Amd.2: 1995 -- Safety requirements for electrical
equipment for measurements, control and laboratory use.
· IEC61000-3-2: 1995 + Amd.1: 1998 + Amd.2: 1998 --- "Electromagnetic compatibility:
Limits for harmonic current emissions (equipment input current up to and including 16A per
phase).
· A "Declaration of Conformity" in accordance with the above standards has been made and
is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (LC), Italy.
FCC Statement (for USA only)
Federal Communications Commission Radio Frequency Interference Statement
W A R N IN G : T his equipm ent generates, uses, and can radiate radio frequency energy. If it is not installed
and used in accordance w ith the instruction m anual, it m ay cause interference to radio
com m unications. It has been tested and found to com ply w ith the lim its for a C lass A com puting
device pursuant to P art 15 of F C C R ules, w hich are intended to provide reasonable protection
against such interference w hen operated in a com m ercial environm ent. O peration of this
equipm ent in a residential area is likely to cause interference, in w hich case the user at his ow n
expense w ill be required to take w hatever m easures m ay be required to correct the interference.

v
Preface
This instruction manual is your guide for using this instrument. It instructs first-time users on
how to use the instrument, and serves as a reference for experienced users.
Before using the instrument, please read this instruction manual carefully, and make sure that the
contents are fully understood. This manual should be easily accessible to the operator at all times
during instrument operation. When not using the instrument, keep this manual in a safe place. If this
instruction manual becomes lost, order a replacement from your local JASCO distributor.
The instruction manual is divided into following volumes:
1) Hardware manual: Describes the principles of operation, specifications,
maintenance, troubleshooting of this instrument.
2) Operation manual: Describes the configuration of this measument program,
functions, and operating procedures.
3) Analysis: Describes the configuration of spectra analysis and file viewer program.
(DS type)
N ote: T he operating procedure varies w ith the type of instrum ent. O peration is described by type. R ead the
relevant portion.

vi
Installation Requirements
To ensure operation safety, observe the following conditions:
(1) Do not operate the instrument under voltage fluctuations exceeding 10% of the
recommended line voltage. Otherwise, the instrument may not function properly.
(2) Frequency or spike noise in the power supply should be minimal.
(3) Ensure that the instrument is grounded.
(4) Operate the instrument in a temperature range of 10 ~ 35°C.
(5) Operate the instrument in a humidity range of 35 ~ 85%(RH). If ambient humidity
exceeds 85%(RH), condensation may deteriorate optical components.
(6) Operate the instrument in an atmospheric pressure range of 950 ~ 1060hPa.
(7) Avoid strong magnetic fields and sources of high frequency. The instrument may not
function properly when near a strong magnetic field or high frequency source.
(8) Avoid vibration from vacuum pumps, electric motors, processing equipment and
machine tools.
(9) Avoid dust and corrosive gas. Do not install the instrument where it may be exposed to
dust, especially in locations exposed to outside air or ventilation outlets that discharge
dust particles.
(10) Do not install the instrument in a location where it may be exposed to direct sunlight.
(11) Install the instrument in a horizontal and stable position. (This includes a table or desk
upon which the instrument is installed.)
(12) Ensure that no air conditioner blows air directly onto the instrument. This may prevent
stable measurement.
(13) Install the instrument in a location that allows easy access for maintenance.
Note: The above conditions do not guarantee optimal performance of this instrument.

vii
Servicing
Contact your local JASCO distributor for instrument servicing. In addition, contact your JASCO
distributor before moving the instrument to another location. Consumable parts should be
ordered according to part number from your local JASCO distributor. If a part number is
unknown, give your JASCO distributor the model name and serial number of your instrument.
Do not return contaminated products or parts that may constitute a health hazard to
JASCO employees.
Notices
(1) JASCO shall not be held liable, either directly or indirectly, for any consequential damage
incurred as a result of product use.
(2) Prohibitions on the use of JASCO software
· Copying software for purposes other than backup
· Transfer or licensing of the right to use software to a third party
· Disclosure of confidential information regarding software
· Modification of software
· Use of software on multiple workstations, network terminals, or by other methods
(not applicable under a network licensing agreement concluded with JASCO)
(3) The contents of this manual are subject to change without notice for product improvement.
(4) This manual is considered complete and accurate at publication.
(5) This manual does not guarantee the validity of any patent rights or other rights.
(6) In general, company names and product names are trademarks or registered trademarks
of the respective companies.
(7) JASCO and the JASCO logo are registered trademarks of JASCO Corporation
ã JASCO Corporation, 2003. All rights reserved. Printed in JAPAN.

viii
Limited Warranty
Products sold by JASCO, unless otherwise specified, are warranted for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects in
the product are found during this warranty period, JASCO will repair or replace the defective
part(s) or product free of charge.
THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE FOLLOWING:
1) IMPROPER OR INADEQUATE INSTALLATION
2) IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT OR
CALIBRATION
3) UNAUTHORIZED MODIFICATION OR MISUSE
4) USE OF CONSUMABLE PARTS NOT SUPPLIED BY AN AUTHORIZED JASCO
DISTRIBUTOR
5) CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR DUE TO
SURROUNDING GASES
6) ACCIDENTS BEYOND JASCO’S CONTROL, INCLUDING NATURAL DISASTERS
This warranty does not cover the consumable parts listed below:
1) Deuterium lamp, tungsten lamp, xenon lamp and other light sources
2) Mirrors in the light source section, and cell windows
3) Fuses, batteries, glassware, chart paper and ink
The LC system only contains:
4) Plunger seals, needle seals, cell window gaskets, valve seals, disk seals and other seal
materials
5) Tubing and fittings (e.g., ferrules, compression screws), and filters (e.g., inlet filters, line
filters, other solvent filters)
6) Pre-columns and guard columns
THE WARRANTY FOR ALL PARTS SUPPLIED AND REPAIRS PROVIDED UNDER THIS
WARRANTY EXPIRES ON THE WARRANTY EXPIRATION DATE OF THE ORIGINAL
PRODUCT. FOR INQUIRIES CONCERNING REPAIR SERVICE, CONTACT YOUR JASCO
DISTRIBUTOR AFTER CONFIRMING THE MODEL NAME AND SERIAL NUMBER OF YOUR
INSTRUMENT.
JASCO Corporation
2967-5, Ishikawa-machi, Hachioji-shi
Tokyo 192-8537
JAPAN

ix
Contents
Safety Considerations................................................................................................................. i
Regulatory Statements.............................................................................................................. iv
Preface ....................................................................................................................................... v
Installation Requirements ........................................................................................................ vi
Servicing .................................................................................................................................. vii
Notices...................................................................................................................................... vii
Limited Warranty.................................................................................................................... viii
1. Introduction ........................................................................................................................... 1
1.1 Layout of this Manual....................................................................................................... 1
1.2 Foreword and Notation Used............................................................................................ 1
1.3 Overview of [Spectra Manager] ....................................................................................... 2
2. Starting and exiting programs and [Spectra Manager] ....................................................... 4
2.1 Startup............................................................................................................................... 4
2.1.1 Turning ON the spectrophotometer........................................................................................ 4
2.1.2 PC and WindowsÒ startup ..................................................................................................... 4
2.1.3 [Spectra Manager] startup ...................................................................................................... 4
2.2 Exiting .............................................................................................................................. 6
2.2.1 Exiting measurement or spectra analysis program ................................................................. 6
2.2.2 PC and spectrophotometer shutdown ..................................................................................... 7
2.3 [Spectra Manager] Menu .................................................................................................. 8
3. Quantitative Analysis and Spectrum Measurement........................................................... 10
3.1 Quantitative Analysis Introduction................................................................................. 10
3.1.1 Quantitative analysis program overview.............................................................................. 10
3.1.1.1 Quantitative analysis program....................................................................................... 10
3.1.1.2 Quantitative analysis operation ..................................................................................... 11
3.1.2 Program startup .................................................................................................................... 11
3.1.3 Calibration curve creation .................................................................................................... 12
3.1.4 Calibration curve modification............................................................................................. 16
3.1.5 Saving quantitative analysis method .................................................................................... 17
3.1.6 Unknown sample measurement............................................................................................ 18
3.1.7 Saving data sheet.................................................................................................................. 19
3.1.8 Printing results...................................................................................................................... 19
3.1.9 Exiting quantitative analysis ................................................................................................ 20
3.2 Spectrum Measurement .................................................................................................. 21
3.2.1 Procedural overview............................................................................................................. 21
3.2.2 [Spectrum measurement] program startup............................................................................ 21
3.2.3 Setting measurement parameters.......................................................................................... 22
3.2.4 Setting the baseline(or Measurement).................................................................................. 23
3.2.5 Sample measurement............................................................................................................ 25
3.2.6 Spectrum save....................................................................................................................... 26
3.2.7 Printing results...................................................................................................................... 26
3.2.8 Instrument shutdown ............................................................................................................ 27
4. [Quantitative Analysis]........................................................................................................ 28

x
4.1 [File] menu ..................................................................................................................... 30
4.1.1 [New...] ................................................................................................................................. 30
4.1.2 [Open...]................................................................................................................................ 32
4.1.3 [Save].................................................................................................................................... 32
4.1.4 [Save As...] ........................................................................................................................... 33
4.1.5 [Page Setup...] ...................................................................................................................... 34
4.1.6 [Print Setup...] ...................................................................................................................... 34
4.1.7 [Print...] ................................................................................................................................ 35
4.1.8 [Exit]..................................................................................................................................... 35
4.2 [Method] menu ............................................................................................................... 36
4.2.1 [New...] ................................................................................................................................. 36
4.2.2 [Open...]................................................................................................................................ 40
4.2.3 [Save As...] ........................................................................................................................... 41
4.2.4 [Modify...]............................................................................................................................. 41
4.2.5 [Information...] ..................................................................................................................... 42
4.3 [Measurement] menu...................................................................................................... 42
4.3.1 [Measurement...]................................................................................................................... 42
4.3.2 [Parameters...]....................................................................................................................... 43
4.3.3 [Blank Correction...]............................................................................................................. 43
4.4 [Edit] menu ..................................................................................................................... 44
4.4.1 [Copy Picture] ...................................................................................................................... 44
4.4.2 [Copy Bitmap]...................................................................................................................... 44
4.4.3 [Copy Text]........................................................................................................................... 44
4.4.4 [Invalid]................................................................................................................................ 44
4.4.5 [Data Input...] ....................................................................................................................... 44
4.4.6 [Title...]................................................................................................................................. 44
4.5 [View] menu ................................................................................................................... 44
4.5.1 [Font...]................................................................................................................................. 45
4.5.2 [Format...]............................................................................................................................. 46
4.5.3 [Cell Width...]....................................................................................................................... 46
4.5.4 [Scale...]................................................................................................................................ 46
4.5.5 [Pattern...]............................................................................................................................. 47
4.5.6 [Grid...]................................................................................................................................. 47
4.5.7 [Style...] ................................................................................................................................ 48
4.5.8 [Marker...]............................................................................................................................. 49
4.6 [Window] menu .............................................................................................................. 49
4.7 [Help] menu.................................................................................................................... 49
5. [Spectrum Measurement].................................................................................................... 50
5.1 [Measurement] menu...................................................................................................... 51
5.1.1 [Start].................................................................................................................................... 51
5.1.2 [Parameter...] ........................................................................................................................ 51
5.1.2.1 [Parameters] dialog box................................................................................................. 51
5.1.2.2 [Data File] dialog box.................................................................................................... 55
5.1.3 [Baseline...]........................................................................................................................... 56
5.1.4 [Move Wavelength...] ........................................................................................................... 58
5.1.5 [Auto Zero]........................................................................................................................... 59
5.1.6 [Exchange Wavelength...]..................................................................................................... 59
5.1.7 [Wavelength for Grating Exchange...].................................................................................. 59
5.1.8 [Exit]..................................................................................................................................... 59
5.2 [Help] menu.................................................................................................................... 59
6. [Time Course Measurement] .............................................................................................. 60
6.1 [Measurement] menu...................................................................................................... 61

xi
6.1.1 [Start].................................................................................................................................... 61
6.1.2 [Parameter...] ........................................................................................................................ 61
6.1.2.1 [Parameter] dialog box .................................................................................................. 61
6.1.2.2 [Data File] dialog box.................................................................................................... 63
6.1.3 [Move Wavelength...] ........................................................................................................... 63
6.1.4 [Auto Zero]........................................................................................................................... 63
6.1.5 [Exit]..................................................................................................................................... 63
6.2 [Help] menu.................................................................................................................... 64
7. [Fixed Wavelength Measurement]...................................................................................... 65
7.1 [Measurement] menu...................................................................................................... 66
7.1.1 [Start].................................................................................................................................... 66
7.1.2 [Blank] ( ) [Blank Correct] ([Correct]) ...................................................................... 66
7.1.3 [Parameter...] ........................................................................................................................ 67
7.1.4 [Goto Wavelength...] ............................................................................................................ 69
7.1.5 [Auto Zero]........................................................................................................................... 69
7.1.6 [Exit]..................................................................................................................................... 69
7.2 [Data] .............................................................................................................................. 69
7.3 [Help] menu.................................................................................................................... 69
8. [Abs/%T Meter] ................................................................................................................... 70
9. [Environment] ..................................................................................................................... 72
9.1 [Hardware Setting] ......................................................................................................... 72
9.2 [Diagnosis] menu............................................................................................................ 73
9.3 [Accessories Setting] ...................................................................................................... 74
9.4 [Wavelength Correction] ................................................................................................ 75
10. Appendix ............................................................................................................................ 76
10.1 Spectra Manager Installation ........................................................................................ 76
10.1.1 Before installation .............................................................................................................. 76
10.1.2 Installing Spectra Manager from Floppy Disk ................................................................... 76
10.1.2.1 Starting up SETUP.EXE.............................................................................................. 76
10.1.2.2 Inputting operator name or company name................................................................. 79
10.1.2.3 Setting the program directory...................................................................................... 80
10.1.2.4 Setting the data directory............................................................................................. 80
10.1.2.5 Copying files to hard disk ........................................................................................... 81
10.1.3 Installing Spectra Manager from CD-ROM................................................................... 82
10.2 Setting the Serial Port (RS-232C) ................................................................................ 84

xii

1
1. Introduction
1.1 Layout of this M anual
T his section describes the layout and function of this m anual. T he V -500 for W indow s
Ò
instruction m anual
consists of 10 sections, including this one. R ead this m anual carefully in order to ensure a full
understanding of the operating procedures before using the V -530/550/560/570.
F or [S pectra A nalysis] program , refer to the "S pectra A nalysis/File V iew er Instruction M anual". For [JA S C O
C anvas] program , refer to the "JA S C O C anvas Instruction M anual".
H ereafter, this m anual w ill refer to the V -500 for W indow s as the V -500W , and to M icrosoft W indow s as
W indow s.
S ection 1. Introduction
T his section explains the w riting syntax and display configuration used in this m anual. R ead this section
first.
S ection 2. S tarting up/exiting program s and [S pectra M anager]
T his section outlines the procedures associated w ith operating the V -500W including starting up the
spectrophotom eter, P C , W indow s, and V -500W , as w ell as exiting V -500W and W indow s and shutting
dow n the spectrophotom eter. S pecific program operations are described in subsequent sections. T his
section also describes the m enu that appears w hen you start up JA S C O [S pectra M anager].
S ection 3. Introduction to quantitative analysis and spectrum m easurem ent
T his section describes quantitative analysis and spectrum m easurem ent. T his section introduces the
inexperienced user to W indow s, spectrophotom etry, and V -500W operation.
S ections 4. to 9. S tandard m easurem ent program reference
T his section provides a reference to explain the functions of each m easurem ent program . It also explains
the procedure for setting instrum ental hardw are and self-diagnostics.
S ection 10. A ppendix
T his section describes how to install the softw are and set the serial port.
1.2 F orew ord and N otation U sed
V -500W runs on W indow s 95/98/N T 4.0/2000, so you need to be fam iliar w ith basic W indow s operations.
T his m anual does not explain how to open m enus, select com m ands, or copy files. If necessary, read the
W indow s docum entation before operating V -500W .

2
T he follow ing notational conventions are used throughout this m anual:
General Notation
Notation Meaning
[Measurement] menu
[Parameters...] command
Names of menus, commands, and text boxes are enclosed in
square brackets [ ], followed by a description indicating whether
the function is a menu, command, text box, or other.
<OK>, <Cancel> Names of buttons are enclosed in angular brackets < >.
Keyboard Operations
Notation Meaning
Shift CTRL Names of keys found on the keyboard are enclosed in boxes.
Alt , F Keys that are to be pressed in succession are separated by commas. In the
example shown on the left, the Alt key is to be pressed and released,
followed by the F key.
Shift + ® Keys that are pressed simultaneously are linked by a plus sign. In the
example shown on the left, press the ® key while holding down the Shift
key.
Mouse Operations
Notation Meaning
Point Move the mouse pointer to the specified item.
Click Quickly press and release the mouse button.
Double-click Click the mouse button twice in rapid succession.
Drag Point to an item, click and hold down the mouse button. Move the mouse
with the button held down, and release the button when the pointer is where
you want it.
1.3 O verview of [S pectra M anager]
T he follow ing program s are registered in the [S pectra M anager] of the M odel V -500W as standard.
M easurem ent program s
(1) [Q uantitative m easurem ent] program
T his program creates a calibration curve by m easuring a standard sam ple w ith know n concentration
according to the com m on quantitative analysis m ethod and m easures an unknow n sam ple to find its
concentration.
(2) [S pectrum m easurem ent] program
T his program obtains the U V /V IS absorption spectrum of a sam ple. T he spectrum m easured by this
program is autom atically transferred to the [S pectra A nalysis] program .
(3) [T im e C ourse M easurem ent] program
T his program m easures the changes in a sam ple w ith tim e at a fixed w avelength. T he tim e course
data obtained by this program is transferred to the [S pectra A nalysis] program .
(4) [F ixed W avelength M easurem ent] program
T his program m easures the absorbance or transm ittance of a sam ple at a fixed w avelength. U p to

3
eight w avelengths can be set and m easured.
(5) [A bs/% T M eter] program
T his program reproduces the analog spectrophotom eter on the C R T screen, so you can read the
absorbance (A bs) or transm ittance (% T ) at an arbitrary w avelength on the m eter.
(6) [E nvironm ent] program
T his program sets the system hardw are, does self-diagnosis, sets optional accessories, and
calibrates w avelengths.
A nalysis program
(1) [S pectra A nalysis] program
T his program saves, prints, and processes (difference spectrum , peak picking, sm oothing, derivative,
vertical axis conversion, and so on) spectrum data or tim e course data.
(2) [F ile V iew er] program
Y ou use this program to search for a spectrum saved on the disk.
(3) [JA S C O C anvas] program
Y ou use this program to lay out and print spectra, m easurem ent param eters, com m ents, and so on.
Y ou can also create draw ings and enter characters.
N ote: This m anual describes the m easurem ent program . For the A nalysis program , refer to the S pectra A nalysis
P rogram M anual.

4
2. Starting and exiting programs and [Spectra Manager]
T his section describes how to start and exit program s and the [S pectra M anager].
2.1 S tartup
2.1.1 Turning O N the spectrophotom eter
T urn O N the pow er sw itch on the right side of the spectrophotom eter.
"Power" Switch
"Power" Lamp
F igure 2.1 S pectrophotom eter (V -530)
W hen the pow er is turned O N , the pow er lam p on the spectrophotom eter is lit.
T he light source needs about five m inutes to becom e stable. T hen m easurem ent m ay begin.
2.1.2 P C and W indow s
Ò
startup
T urn O N the pow er sw itches for the P C and C R T . M icrosoft W indow s should start autom atically. If
necessary, refer to the M icrosoft W indow s instruction m anual
2.1.3 [S pectra M anager] startup
(1) W hen W indow s is started, [Jasco]-[S pectra M anager] also starts. (S ee F ig. 2.2.) T he [S pectra
M anager] w indow appears. (S ee Fig. 2.3.)

5
F igure 2.2 W indow s screen
F igure 2.3 [S pectra M anager] w indow
(2) In the [S pectra M anager] w indow , m ake sure the Instrum ent in the [Instrum ents:] box is the
spectrophotom eter you are using. If not, click the arrow at the right side of the box and select the
correct spectrophotom eter. T he w indow changes to the m enu for that spectrophotom eter.
N ote: T he [S pectra M anager] w indow displays the available program s for the spectrophotom eter. F ig. 2.3 show s
an exam ple of the standard program m enu. If an optional application program has been included, it
w ill also appear in the [S pectra M anager] w indow . S ee S ection 2.3, [S pectra M anager] M enu for a full
explanation.
(3) D ouble-click a program on the m enu. T he selected program starts and the program w indow appears.
T he spectrophotom eter also starts autom atically, but needs about tw o m inutes to w arm up.
M essages appear throughout the procedure. F or exam ple, w hen the [S pectrum M easurem ent]
program is started, the [S pectrum M easurem ent] display show n in Fig. 2.5 appears after the display
show n in F ig. 2.4 appears.
N ote: S ee S ection 3 Introduction for a full description of the M easurem ent program operation.

6
F igure 2.4 M essage boxes during startup
N ote: C heck the follow ing if the m essage on the right in Fig. 2.4 appears.
· P ow er to the spectrophotom eter is turned O N .
· T he spectrophotom eter and P C are correctly interfaced.
· T he P C environm ent (for exam ple, com m unication port) is correctly set.
F igure 2.5 [S pectrum M easurem ent] display
2.2 E xiting
T his section describes how to exit the [M easurem ent] or [A nalysis] program s and how to shut dow n the
spectrophotom eter and P C .
2.2.1 E xiting m easurem ent or spectra analysis program
E nds the [M easurem ent] or [ A nalysis] program and exits it.
(1) E xiting the [S pectra A nalysis] program
C lick [F ile] - [E xit]. T he [S pectra A nalysis] w indow closes and the [S pectrum M easurem ent] w indow
appears.
N ote: If a spectrum has not been saved, a m essage appears to inform the operator. P roceed according to the
m essage. A m essage appears for each unsaved spectrum . R epeat procedure accordingly.
(2) E xiting the [S pectrum M easurem ent] program
C lick [M easurem ent] - [E xit]. T he [S pectrum M easurem ent] w indow closes and the [S pectra

7
M anager] w indow appears.
(3) E xiting the [S pectra M anager] program
C lick [A pplications] - [E xit].
(4) E xiting W indow s
E xit W indow s according to the W indow s U ser
’
s G uide.
2.2.2 P C and spectrophotom eter shutdow n
(1) T urn O FF the pow er to the P C and display. D o not forget to turn off the display.
(2) C heck that the sam ple cham ber is em pty. T hen turn O F F the pow er to the spectrophotom eter.