Jasco P-1010 User manual

P/N: 0302-0411A April 1999
P-1010/1020/1030
Polarimeter
Hardware/Function Manual
(Data Station Type)

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Safety Considerations
To ensure operation safety, this instrument must be operated correctly and maintained
regularly according to schedule. Carefully read to fully understand all safety precautions in
this manual before operating the instrument. This manual denotes precautions against
actions that can result in hazardous situations or equipment damage by using the signal
words WARNING, CAUTION, and Note.
(1) Safety symbols
Instruction manual symbol. If the product is marked with this symbol, refer to
the instrument manuals to protect the instrument against damage.
WARNING A WARNING indicates an potentially hazardous situation which, if not
avoided, could result in death or serious injury
.
CAUTION A CAUTION indicates a potentially hazardous situation which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against
unsafe practices.
Do not proceed beyond a WARNING or CAUTION notice until you
understand the hazardous conditions and have taken the appropriate steps.
Note ANote provides additional information to aid the operator in obtaining
optimal instrument performance.

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(2) Warning Label
Warning labels are attached at several locations on this instrument. Do not remove,
deface or damage the warning labels. If a warning label peels off the instrument or
becomes illegible, contact your local JASCO distributor for a replacement label. Be
sure to indicate the part No. on the label.
1) Warning for FUSE (on Figure 1, 2 or 3)
WARNING
FOR CONTINUED
PROTECTION AGAINST
RISK OF FIRE, REPLACE
ONLY WITH FUSE OF THE
SPECIFIED TYPE AND
CURRENT RATINGS.
Part No.：0822-0102A
2) Warning for GROUND (on Figure 1, 2 or 3)
WARNING
MAINS PLUG GROUND
PIN OR GROUND
TERMINAL MUST BE
CONNECTED TO
GROUND.
Part No.：0822-0104A
Power
On
Off
AC Input
T3.1 5A( 10 0- 1 20V )
T2A (200-240V)
FUS E
Serial
iRM PS
AUX1
AUX2
LC Out
Temp. Contr ol
Analog
Out
GND
GND
1
2
Figure 1 Rear panel of P-1010
Only use fuses of the designated rating to protect
both operator and instrument from fire and other
hazards. The warning labels that pertain to fuse
ratings are located on the back panel of the
instrument .
Be sure to ground the mains plug ground pin.
Otherwise, ground the ground terminal on the
back panel of the instrument.

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Power
On
Off
50Hz
60Hz
AC Input
T5A (100-120V)
T3.1 5A( 20 0- 2 40V )
FUS E
iRM PS
AUX1
AUX2
LC Out
Temp. Contr ol
Analog
Out
Se
ri
a
l
GND
GND
1
2
Figure 2 Rear panel of P-1020
iRM PS
AUX1
AUX2
LC Out
Temp. Contr ol
Analog
Out
FUSE
T 10 A ( 100- 120V )
T 5A (20 0-240V)
AC Input
Power
On
Off
50Hz
60Hz
Hg
Na
Serial
GND
GND
1
2
Figure 3 Rear panel of P-1030

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3) Warning for ULTRAVIOLET RADIATION (on Figure 4)
Lamp house lid
Bottom inside lamp house
1
2
Figure 4 Left side panel
(1) Precautions
1) Always keep the polarimeter clean. Take particular care to keep the sample chamber
clean and do not leave sample cells in the chamber. When measuring samples that
may generate harmful gas, cover and remove the cell from the sample chamber
immediately after measurement.
2) If the instrument must be left for about an hour, be sure to leave the lamp on. The
service life is shortened when the lamp is frequently turned on and off. Note that the
lamp requires about 15 minutes to stabilize after being turned on.
3) Always keep the cells clean. Clean a cell immediately after use and store in a clean,
dust-free location.
4) When sample has spilled in the sample chamber, wipe out immediately using gauze or
other material. When acids or bases have been spilled, wipe out with gauze soaked in
water then allow to dry.
5) When the optical rotation of a sample exceeds ±50×, the correct optical rotation may
not be shown if the sample with the large optical rotation is placed in the sample
chamber first. In this case, use the following procedure.
1 Beforehand, prepare a known sample with an appropriate optical rotation range of the
+30 ∼+50 °or -30 ∼-50 °. As known samples, + and - polarizing plates are supplied
as special accessories. Also, saccharose solution (concentration of about 50%, +) or
fructose sugar (concentration of about 50%, -) can be used as easy samples. Place
the samples in the cell with a 100mm light path length and use.
2 when the optical rotation if the unknown sample to be measured is to the + side, set the
known sample with a + optical rotation prepared above in the sample chamber,
measure, and let the optical rotation value stabilize to +30 ∼+50 °.
WARNINH
ULTRAVIOLET RADIATION
WHEN Hg LAMP IS USED, HARMFUL
ULTRAVIOLET RADIATION IS EMITTED
THERERORE, TURN THE POWER SWITCH
OFF BEFORE OPENING THIS COVER.
Part No.：0822-0116A
4) Warning for HIGH
TEMPERATURE (on Figure 4)
WARNINH
HIGH TEMPERATURE
THE LAMP IS STILL VERY HOT
WHEN THE POWER SWITCH IS
TURNED OFF THEREFORE, WAIT
AT LEAST FIVE MINUTES BEFORE
TOUCHING THE LAMP.
Part No.：0822-0118A

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3 Next, in this condition, open the lit of the sample chamber, replace the known sample
with the unknown sample will then be shown.
Note: Once the lid of the sample chamber has been opened, do not close the lid until the
known sample has been replaced by the unknown sample.
4 After measuring the unknown sample, open the sample chamber lid, replace the
unknown sample with the known sample used above, close the lid, and measure until
the optical rotation stabilizes at +30 ∼+50 °.
5 Remove the known sample from the sample chamber and close the lid. The
measurement value will return to zero.
Note : When the sign of the optical rotation of the unknown sample is minus, use the same
operations with the known sample that is also minus.

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Regulatory Statements
CE Notice
Marking by the symbol indicates compliance of this JASCO system to the EMC
(Electromagnetic Compatibility) and Low Voltage Directives of the European Community.
This symbol indicates that this JASCO system meets the relevant basic safety and health
requirements of the EC Directive based on the following technical standards:
・EN55011 ---- “Limits and Methods of Measurement of Radio Interference Characteristics
of Information Technology Equipment.” ---- Group 1, Class A.
WARNING: This is a Class A product. In a domestic environment this product may
cause radio interference, in which case the user may be required to
take adequate measures.
・EN50082-1 ---- “Electromagnetic compatibility ---- Generic immunity standard Part 1:
Residential, commercial, and light industry.”
・IEC1000-4-2 ---- “Electromagnetic compatibility for industrial-process measurement and
control equipment Part 2: Electrostatic discharge requirements.” ---- Severity level 3.
・IEC1000-4-3 ---- “Electromagnetic compatibility for industrial-process measurement and
control equipment Part 3: Radiated electromagnetic field requirements.” Severity level 2.
・IEC1000-4-4 ---- “Electromagnetic compatibility for industrial-process measurement and
control equipment Part 4: Electrical fast transient/burst requirements.” ---- Severity level 2.
・IEC1010-1: 1990 + Amd.1:1992 + Amd.2: 1995 ---- Safety requirements for electrical
equipment for measurements, control and laboratory use.
・A “Declaration of Conformity” in accordance with the above standards has been made and
is on file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (Lc), Italy.

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Preface
This instruction manual serves as your guide book for using this instrument. It is intended to
instruct first-time users on how to properly use the instrument, and to serve as a reference
for experienced users.
Before using the instrument, please read this instruction manual carefully, and make sure you
fully understand its contents. This manual should be easily accessible to the operator at all times
during instrument operation. When not using the instrument, keep this manual stored in a safe
place. Should this instruction manual be lost, order a replacement from your local JASCO
distributor.

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viii
Installation Conditions
To ensure operation safety, observe the following conditions:
(1) Do not operate the instrument under voltage fluctuations exceeding 10% of the
recommended line voltage. Otherwise, the instrument may not function properly.
(2) Use a three-prong electrical outlet with a ground.
(3) Operate the instrument under a temperature range of 10∞∼35∞C.
(4) Operate the instrument under a humidity range of 35% ∼85%(RH). If ambient
humidity exceeds 85%(RH), condensation may deteriorate optical components.
(5) Operate the instrument under atmospheric pressure of 750 ∼1060hPa.
(6) Avoid strong magnetic fields and sources of high frequency. The instrument may not
function properly when near a strong magnetic field or high frequency source.
(7) Avoid vibration from vacuum pumps, electric motors, processing equipment and
machine tools.
(8) Avoid dust and corrosive gas. Do not install the instrument where exposed to dust,
especially in locations exposed to outside air or ventilation outlets that discharge dust
particles.
(9) Do not install the instrument where exposed to direct sunlight.
(10) Ensure that no air conditioner blows air directly on the instrument. This may inhibit
stable measurement.
Note: The above conditions do not ensure optimal performance of this instrument.

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Servicing
To service your instrument, contact your local JASCO distributor. Also contact your JASCO
distributor before moving the instrument to another location.
Consumable parts should be ordered according to part Nos. from your local JASCO
distributor. If a part No. is unknown, give your JASCO distributor the model name and serial
No. of your instrument.
Do not return contaminated products and parts that may constitute health
hazards to JASCO employees.
Notice
(1) JASCO SHALL NOT BE HELD LIABLE, EITHER DIRECTLY OR INDIRECTLY, FOR
ANY CONSEQUENTIAL DAMAGE INCURRED AS A RESULT OF PRODUCT USE.
(2) PROHIBITIONS ON THE USE OF JASCO SOFTWARE
COPYING SOFTWARE FOR PURPOSES OTHER THAN BACKUP
TRANSFER OR LICENSING OF THE RIGHT TO USE SOFTWARE TO A THIRD
PARTY
DISCLOSURE OF CONFIDENTIAL INFORMATION REGARDING SOFTWARE
MODIFICATION OF SOFTWARE
USE OF SOFTWARE ON MULTIPLE WORKSTATIONS, NETWORK TERMINALS, OR
BY OTHER METHODS (NOT APPLICABLE UNDER A NETWORK LICENSING
AGREEMENT CONCLUDED WITH JASCO)
(3) THE CONTENTS OF THIS MANUAL ARE SUBJECT TO CHANGE WITHOUT
NOTICE FOR PRODUCT IMPROVEMENTS.
(4) THIS MANUAL IS CONSIDERED COMPLETE AND ACCURATE AT PUBLICATION.
(5) THIS MANUAL DOES NOT GUARANTEE THE VALIDITY OF ANY PATENT RIGHTS
OR OTHER RIGHTS.
(6) IN GENERAL, COMPANY NAMES AND PRODUCT NAMES ARE TRADEMARKS OR
RESISTARED TRADMARKS OF THE RESPECTIVE COMPANIES.
(7) JASCO AND THE JASCO LOGO ARERESISTERED TRADEMARKS OF JASCO
CORPORATION.

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LIMITED WARRANTY
Products sold by JASCO, unless otherwise specified, are warranted for a period of one year
from the date of shipment to be free of defects in materials and workmanship. If any defects
in the product are found during this warranty period, JASCO will repair or replace the
defective part(s) or product free of charge.
THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE
FOLLOWING:
1) IMPROPER OR INADEQUATE INSTALLATION
2) IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT OR
CALIBRATION
3) UNAUTHORIZED MODIFICATION OR MISUSE
4) USE OF CONSUMABLE PARTS NOT SUPPLIED BY AN AUTHORIZED JASCO
DISTRIBUTOR
5) CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR DUE
TO SURROUNDING GASES
6) ACCIDENTS BEYOND JASCOíS CONTROL, INCLUDING NATURAL DISASTERS
This warranty does not cover the consumable parts listed below:
1) Deuterium lamp, tungsten lamp, xenon lamp and other light sources
2) Mirrors in the light source section, and cell windows
3) Fuses, batteries, glassware, chart paper and ink
The LC system only contains:
4) Plunger seals, needle seals, cell window gaskets, valve seals, disk seals and other
seal materials
5) Tubing and fittings (e.g., ferrules, compression screws), and filters (e.g., inlet filters, line
filters, other solvent filters)
6) Pre-columns and guard columns
THE WARRANTY FOR ALL PARTS SUPPLIED AND REPAIRS PROVIDED UNDER THIS
WARRANTY EXPIRES ON THE WARRANTY EXPIRATION DATE OF THE ORIGINAL
PRODUCT. FOR INQUIRIES CONCERNING REPAIR SERVICE, CONTACT YOUR JASCO
DISTRIBUTOR AFTER CONFIRMING THE MODEL NAME AND SERIAL NUMBER OF
YOUR INSTRUMENT.
JASCO Corporation
2967-5, Ishikawa-cho, Hachioji-shi
Tokyo 192, JAPAN

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Contents
Safety Considerations ........................................................................ i
Regulatory Statements..................................................................... vi
Preface .............................................................................................vii
Installation Conditions .....................................................................viii
Servicing........................................................................................... ix
LIMITED WARRANTY....................................................................... x
1. General Description and Specifications ....................................... 1
1.1 General Description...................................................................................1
1.2 Specifications.............................................................................................1
1.2.1 P-1010 Polarimeter..............................................................................1
1.2.2 P-1020 Polarimeter..............................................................................2
1.2.3 P-1030 Polarimeter..............................................................................2
1.3 Hardware Structure....................................................................................3
2. Names and Functions of Each Part.............................................. 5
2.1 Overview....................................................................................................5
2.2 Rear panel .................................................................................................5
2.3 Filter...........................................................................................................8
3. Maintenance................................................................................. 9
3.1 Cautions in Use .........................................................................................9
3.2 Cleaning Sample Chamber .......................................................................9
3.2.1 Dismounting the sample cell unit..........................................................9
3.2.2 Cleaning the sample cell unit..............................................................10
3.2.3 Remounting the sample cell unit ........................................................10
3.3 Consumables...........................................................................................11
3.4 Performance Check.................................................................................12
3.5 Replacing Light Sorce .............................................................................14
3.5.1 Replacing of Halogen Lamp for P-1010 .............................................14
3.5.2 Replacing of Lamp for P-1020............................................................14
3.5.3 Replacing of Lamp for P-1030............................................................15
3.5.4 Replacing the Glow Discharge Tube ..................................................16
3.6 Replacing Fuses......................................................................................17

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1. General Description and Specifications
1.1 General Description
The Model P-1010/1020/1030 are polarimeters designed to measure the optical
rotation of optically active substances. These instruments also enable the direct
measurement of specific optical rotation, concentrations, sugar scales, Brix purity
and optical purity. Substances that that can be measured include saccharides,
amino acids, pharmaceuticals, perfumes and many other physiologically active
substances. These instruments are also used for the quantitative analysis
discrimination of D-optical isomer, L-optical isomer and others.
1.2 Specifications
1.2.1 P-1010 Polarimeter
Light source Halogen lamp (WI)
Polarizer Polaroid
Faraday cell Pb glass
Measurement wavelength 589 nm
Maximum cell length 100 mm
Detector Photomultiplier tube
Printer Equipped with Centronics parallel interface
Display Liquid crystal panel
Language Japanese, English
Temperature measurement Within cell holder/cell (sample)
Temperature measurement range 0∼40°C (Minimum display temperature: 0.1°C)
Measurement method Optical zero method with symmetric angle oscillation
Measurement Angle range ±90°
Minimum display angle 0.0002°
Response speed 6°/sec.
Integration time 1∼100 sec.
Measurement accuracy ±0.002°(±1°or less), 0.2% (more than ±1°)
Measurement repeatability ±0.002°
Data modes Optical rotation, Specific optical rotation ,
Concentration, Sugar scale,
Brix purity, Optical purity
Data processing Edit data
Statistical calculation: average value,
standard deviation
coefficient of variation
Other functions Recognizes light source, filter
External terminal External start, External auto zero
External output Analog output: ±1V±1%, full scale: 1°, 10°, 100°
LC output: 0.5°→5V±3% : 589 nm (WI)
Dimensions 563(W)×350(D) ×210(H)mm
Weight 27 kg
Power requirements 100, 115, 200, 220, and 240 VAC ±10%,
50/60 Hz, 80 W

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1.2.2 P-1020 Polarimeter
Light source Sodium lamp (Na)
Polarizer Polaroid
Faraday cell Pb glass
Measurement wavelength 589 nm
Maximum cell length 100 mm
Detector Photomultiplier tube
Printer Equipped with Centronics parallel interface
Display Liquid crystal panel
Language Japanese, English
Temperature measurement Within cell holder/cell (sample)
Temperature measurement range 0∼40°C (Minimum display temperature: 0.1°C)
Measurement method Optical zero method with symmetric angle oscillation
Measurement Angle range ±90°
Minimum display angle 0.0002°
Response speed 6°/sec.
Integration time 1∼100 sec.
Measurement accuracy ±0.002°(±1°or less), 0.2% (more than ±1°)
Measurement repeatability ±0.002°
Data modes Optical rotation, Specific optical rotation ,
Concentration, Sugar scale,
Brix purity, Optical purity
Data processing Edit data
Statistical calculation: average value,
standard deviation
coefficient of variation
Other functions Recognizes light source, filter
External terminal External start, External auto zero
External output Analog output: ±1V±1%, full scale: 1°, 10°, 100°
LC output: 0.5°→5V±3%：589 nm (Na)
Dimensions 563(W)×350(D) ×210(H)mm
Weight 33 kg
Power requirements 100, 115, 200, 220, and 240 VAC ±10%,
50/60 Hz, 270 W
1.2.3 P-1030 Polarimeter
Light source Sodium lamp (Na), Mercury lamp (Hg)
Polarizer Glan-Taylor polarizing prism
Faraday cell Pb glass
Measurement wavelength 589, 577, 546, 435, 365 nm
Light source exchange Computer control
Maximum cell length 100 mm
Detector Photomultiplier tube
Printer Equipped with Centronics parallel interface
Display Liquid crystal panel
Language Japanese, English
Temperature measurement Within cell holder/cell (sample)
Temperature measurement range 0∼40°C (Minimum display temperature: 0.1°C)
Measurement method Optical zero method with symmetric angle oscillation

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Measurement Angle range ±90°
Minimum display angle 0.0002°
Response speed 6°/sec.
Integration time 1∼100 sec.
Measurement accuracy ±0.002°(±1°or less), 0.2% (more than ±1°)
Measurement repeatability ±0.002°
Data modes Optical rotation, Specific optical rotation ,
Concentration, Sugar scale,
Brix purity, Optical purity
Data processing Edit data
Statistical calculation: average value,
standard deviation
coefficient of variation
Other functions Recognizes light source, Recognizes filter
External terminal External start, External auto zero
External output Analog output: ±1V±1%, full scale:1°, 10°, 100°
LC output: 0.5°→5V±3%：589 nm (Na)
Dimensions 563(W) ×350(D) ×210(H)mm
Weight 39.5 kg
Power requirements 100, 115, 200, 220, and 240 VAC ±10%,
50/60 Hz, 530 W
1.3 Hardware Structure
Figure 1.1 shows a block diagram of the polarimeter. Light from the light source
(lamp) passes through slit S, lens L, and filter F before entering polarizer P. The light
from the light source up through filter F is natural. This natural light is linearly
polarized by polarizer P. The linearly polarized light then passes through modulator
M, the sample chamber, and analyzer A before entering the photomultiplier tube PM
for detection.
The light incident on the detector (PM) is converted into an electrical signal, then
converted into a digital signal by the A/D converter for input to the microcomputer.
The signal processed by the microcomputer is output to the iRM display as optical
rotation, specific optical rotation, concentration, sugar scale, Brix purity or optical
purity according to the set data mode.

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SO
S
L
F
P
M
Sample
A
PM
Pre
AMP
Generator
Ref.
AM
P
H.T.
AMP
Main
AMP
Servo
AMP
Accessory
Micro
Computer
Servo
Motor
G
PC
RS-232C
SO: Light source S: Slit L: Lens F: Filter
P: Polarizer M: Modulator A: Analyzer
PM: Photomultiplier tube G: Gear
Figure 1.1 Block diagram

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2. Names and Functions of Each Part
2.1 Overview
Main unit
2
3
4
1
Figure 2.1 Overview
Name
function
Lamp housing
Filter unit
Sample chamber
“Power” lamp
The lamp is housed here.
Contains a filter for changing over wavelength.
Includes the cell holder, light beam aperture changer, and
sensor for temperature measurement.
The aperture changer can be set at 8, 3, or 1.8 mm.
Goes on when the power switch (shown in Figure 2.2, 2.3 and
2.4) is turned on.
2.2 Rear panel
P-1010 rear panel
Figure 2.2 P-1010 rear panel

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P-1020 rear panel
Power
On
Off
50Hz
60Hz
AC Input
T5A (100-120V)
T3. 15 A( 20 0- 2 40V )
FUSE
iRMPS
Serial
AUX1
AUX2
LC Out
Tem p. Contr o l
Analog
Out
GND
GND
1
2
3
4
5
7
8
9
10
11
6
Figure 2.3 P-1020 rear panel
P-1030 rear panel
iRMPS
FUS E
T10 A (10 0- 12 0V)
T5A (200-240V)
AC Input
Power
On
Off
50Hz
60Hz
Hg
Na
Serial
AUX1
AUX2
LC Out
Tem p. Contr ol
Analog
Out
GND
GND
2
3
4
5
1
6
7
8
9
10
11
Figure 2.4 P-1030 rear panel

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Name
Function
“Power” switch
Frequency
change switch
Ground terminal
“AC Input” connector
“FUSE”
“iRM PS” connector
“Serial” connector
“AUX1”, “AUX2”
connector
“Analog Out” terminal
“Temp. Control”
connector
11
“LC Out” terminal
Power switch
Matches to the frequency of the power line.
Be sure to ground this terminal.
Connects the power cable.
The specified fuse for the voltage is contained here. Be
sure to match the voltage of the power source being
used.
Enables power to be supplied to the intelligent remote
module (iRM). Connector for connecting cable.
Connected to the ²COM 1 ²connector on PC.
Connector for connecting the quick flow sampler
(optional).
Outputs a 3 digit angle component corresponding to the
set measurement values in units of mV during normal
measurement.
Connector for the optional temperature controller.
Outputs a direct current voltage using 0.1°/V in LC
mode.

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2.3 Filter Unit
Remove the lid from the filter unit and replace the filter (see Fig. 2.5).
589
nm
Front of the instrument
Filter
Figure 2.5 Top view of filter unit
Note: Lightly pressing the left and right ends of the disk pops up the lid of the filter
unit.
The filter must be inserted in a specified direction. Insert it by noting the
wavelength indication at the top of the filter, as illustrated. If it is inserted an
incorrect orientation, ! ERROR ! will appear.
To keep out dust and foreign matter and to prevent light leakage, keep the lid
closed except when replacing.