Jasco V-530 User manual

P/N:0302-0256B November 2000
Model V-530/550/560/570
UV/VIS Spectrophotometer
Hard ware/Function Manual

i
Safet Considerations
To ensure operation safety, this instrument must be operated orre tly and maintained a ording to
a regular s hedule. Carefully read to fully understand all safety pre autions in this manual before
operating the instrument. Please take a moment to understand what the signal words WARNING!,
CAUTION, and Note mean in this manual.
(1) Safet s mbols
Instru tion manual symbol. If the produ t is marked with this symbol, refer to the
instrument manuals to prote t the instrument against damage.
WARNING! A WARNING! indi ates a potentially hazardous situation whi h, if not avoided,
ould result in serious injury or even death.
CAUTION A CAUTION indi ates a potentially hazardous situation whi h, if not avoided, may
result in minor or moderate injury. It may also be used to alert against damaging
the equipment.
Do not pro eed beyond a WARNING! or CAUTION noti e until you understand
the hazardous onditions and have taken the appropriate steps.
Note: A Note provides additional information to help the operator achieve optimal
instrument performance.

ii
(2) Warning Label
Warning labels are atta hed at several lo ations on this instrument. Do not
remove, defa e or damage the warning labels. If a warning label peels off the
instrument or be omes illegible, onta t your lo al JASCO distributor for a
repla ement label. Be sure to indi ate the part number on the label.
1) Warning for FUSE Fig.1 or Fig. 2
Part No.0822-xxxxA
2) Warning for GROUND Fig.1or Fig.2
Part No.0822-xxxxA
Warning for FUSE Warning for GROUND
Figure 1 Warning Labels on the V-530 Rear Panel
!
!!
!WARNING
FOR CONTINUED PROTECTION
AGAINST RISK OF FIRE, REPLACE
ONLY WITH FUSE OF THE SPECIFIED
TYPE AND CURRENT RATINGS.
Only use fuses of the designated rating to
prote t both operator and instrument from fire
and other hazards. When repla ing a fuse,
refer to the hardware manual (4.4.1 Repla ing
the fuse). The warning labels that pertain to
fuse ratings are lo ated on the rear panel of
the instrument.
!
!!
!WARNING
MAINS PLUG GROUND PIN
OR GROUND TERMINAL
MUST BE CONNECTED
TO GROUND.
Be sure to ground the mains plug ground pin.
Otherwise, ground the ground terminal on the
rear panel of the instrument.

iii
Warning for FUSEWarning for GROUND
Figure 2 Warning Labels on the V-550/560/570 Rear Panel
(2) Warning for carr ing
These instruments weigh as follows:
V-530: 16.0 kg V-550: 30.5 kg
V-560: 32.0 kg V-570: 33.0 kg
When arrying the instrument, hold the arrying handle at the bottom of the
instrument firmly (See Fig 3).
Carrying handle
Figure 3 Example: V-550/560/570 side view

iv
Regulator Statement
CE Notice
The symbol indi ates omplian e of this JASCO system to the EMC (Ele tromagneti
Compatibility) and Low Voltage Dire tives of the European Community. This symbol indi ates that
this JASCO system meets the relevant basi safety and health requirements of the EC Dire tive
based on the following te hni al standards:
• EN55011 -- "Industrial, s ientifi and medi al (ISM) radio-frequen y equipment – Radio
disturban e hara teristi s – Limits and methods of measurement" -- Group 1, Class A.
WARNING!: This is a Class A produ t. In a domesti environment this produ t may
ause radio interferen e, in whi h ase the user may be required to take
orre tive measures.
• EN61000-6-1 -- "Ele tromagneti ompatibility (EMC) Part 6-1:Generi standards – Immunity
for residential, ommer ial and light-industrial environments"
• IEC61000-3-2 -- "Ele tromagneti ompatibility (EMC) Part 3-2:Limits – Limits for harmoni
urrent emissions (equipment input urrent up to and in luding 16A per phase) "
• IEC61010-1 -- "Safety requirements for ele tri al equipment for measurement, ontrol and
laboratory use – Part 1:General requirements"
• A "De laration of Conformity" in a ordan e with the above standards has been made and is on
file at JASCO EUROPE srl, Via Confalonieri 25, 22060 CREMELLA (LC), Italy.
FCC Statement (for USA only)
Federal Communi ations Commission Radio Frequen y Interferen e Statement
WARNING!: This equipment generates, uses, and an radiate radio frequen y
energy. If it is not installed and used in a ordan e with the instru tion
manual, it may ause interferen e to radio ommuni ations. It has been
tested and found to omply with the limits for a Class A omputing devi e
pursuant to Part 15 of FCC Rules, whi h are designed to provide
reasonable prote tion against su h interferen e when operated in a
ommer ial environment. Operation of this equipment in a residential
area is likely to ause interferen e, in whi h ase the user at his own
expense will be required to take whatever measures may be required to
orre t the interferen e.

v
Preface
This instru tion manual is your guide for using this instrument. It instru ts first-time users on how to
use the instrument, and serves as a referen e for experien ed users.
Before using the instrument, please read this instru tion manual arefully, and make sure that the
ontents are fully understood. This manual should be easily a essible to the operator at all times during
instrument operation. When not using the instrument, keep this manual in a safe pla e. If this instru tion
manual be omes lost, order a repla ement from your lo al JASCO distributor.

vi
Installation Requirements
To ensure operation safety, observe the following onditions:
(1) Do not operate the instrument under voltage flu tuations ex eeding 10% of the
re ommended line voltage. Otherwise, the instrument may not fun tion properly.
(2) Frequen y or spike noise in the power supply should be minimal.
(3) Ensure that the instrument is grounded.
(4) Operate the instrument in a temperature range of 10 – 35 C.
(5) Operate the instrument in a humidity range of 35 - 85% (RH). If ambient humidity ex eeds
85% (RH), ondensation may deteriorate opti al omponents.
(6) Operate the instrument in an atmospheri pressure range of 750 ∼ 1060hPa.
(7) Avoid strong magneti fields and sour es of high frequen y. The instrument may not
fun tion properly when near a strong magneti field or high frequen y sour e.
(8) Avoid vibration from va uum pumps, ele tri motors, pro essing equipment and ma hine
tools.
(9) Avoid dust and orrosive gas. Do not install the instrument where it may be exposed to dust,
espe ially in lo ations exposed to outside air or ventilation outlets that dis harge dust
parti les.
(10) Do not install the instrument in a lo ation where it may be exposed to dire t sunlight.
(11) Install the instrument in a horizontal and stable position. (This in ludes a table or desk upon
whi h the instrument is installed.)
(12) Ensure that no air onditioner blows air dire tly onto the instrument. This may prevent
stable measurement.
(13) Install the instrument in a lo ation that allows easy a ess for maintenan e.
Note: The a ove conditions do not guarantee optimal performance of this
instrument.

vii
Servicing
Conta t your lo al JASCO distributor for instrument servi ing. In addition, onta t your JASCO
distributor before moving the instrument to another lo ation. Consumable parts should be ordered
a ording to part number from your lo al JASCO distributor. If a part number is unknown, give your
JASCO distributor the model name and serial number of your instrument.
Do not return contaminated products or parts that may constitute a health hazard to JASCO
employees.
Notices
(1) JASCO shall not be held liable, either dire tly or indire tly, for any onsequential damage
in urred as a result of produ t use.
(2) Prohibitions on the use of JASCO software
 Copying software for purposes other than ba kup
 Transfer or li ensing of the right to use software to a third party
 Dis losure of onfidential information regarding software
 Modifi ation of software
 Use of software on multiple workstations, network terminals, or by other methods (not
appli able under a network li ensing agreement on luded with JASCO)
(3) The ontents of this manual are subje t to hange without noti e for produ t improvement.
(4) This manual is onsidered omplete and a urate at publi ation.
(5) This manual does not guarantee the validity of any patent rights or other rights.
(6) If a JASCO software program has failed ausing an error or improper operation, this may be
aused by a onfli t from another program operating on the PC. In this ase, take orre tive
a tion by uninstalling the onfli ting produ t(s).
(7) In general, ompany names and produ t names are trademarks or registered trademarks of
the respe tive ompanies.
(8) JASCO and the JASCO logo are registered trademarks of JASCO Corporation
(C) JASCO Corporation, 2000. All rights reserved. Printed in JAPAN.

viii
Limited Warrant
Produ ts sold by JASCO, unless otherwise spe ified, are warranted for a period of one year from
the date of shipment to be free of defe ts in materials and workmanship. If any defe ts in the
produ t are found during this warranty period, JASCO will repair or repla e the defe tive part(s) or
produ t free of harge.
THIS WARRANTY DOES NOT APPLY TO DEFECTS RESULTING FROM THE FOLLOWING:
(1) MPROPER OR INADEQUATE INSTALLATION
(2) IMPROPER OR INADEQUATE OPERATION, MAINTENANCE, ADJUSTMENT OR
CALIBRATION
(3) UNAUTHORIZED MODIFICATION OR MISUSE
(4) USE OF CONSUMABLE PARTS NOT SUPPLIED BY AN AUTHORIZED JASCO
DISTRIBUTOR
(5) CORROSION DUE TO THE USE OF IMPROPER SOLVENTS, SAMPLES, OR DUE TO
SURROUNDING GASES
(6) ACCIDENTS BEYOND JASCO'S CONTROL, INCLUDING NATURAL DISASTERS
This warranty does not over the onsumable parts listed below:
(1) Tungsten lamp, and other light sour es
(2) Mirrors in the light sour e se tion, and ell windows
(3) Fuses, batteries, glassware, hart paper and ink
THE WARRANTY FOR ALL PARTS SUPPLIED AND REPAIRS PROVIDED UNDER THIS
WARRANTY EXPIRES ON THE WARRANTY EXPIRATION DATE OF THE ORIGINAL
PRODUCT. FOR INQUIRIES CONCERNING REPAIR SERVICE, CONTACT YOUR JASCO
DISTRIBUTOR AFTER CONFIRMING THE MODEL NAME AND SERIAL NUMBER OF YOUR
INSTRUMENT.
JASCO Corporation
2967-5, Ishikawa-ma hi, Ha hioji-shi
Tokyo 192-8537
JAPAN

ix
Contents
Sa ety Considerations...........................................................................................................i
Regulatory Statement.......................................................................................................... iv
Pre ace...................................................................................................................................... v
Installation Requirements .................................................................................................. vi
Servicing ................................................................................................................................vii
Notices.................................................................................................................................... vii
Limited Warranty.................................................................................................................viii
1 Speci ications......................................................................................................................1
1.1 V-530 UV/VIS Spectrophotometer Specifications..................................................... 1
1.2 V-550 UV/VIS Spectrophotometer Specifications..................................................... 2
1.3 V-560 UV/VIS Spectrophotometer Specifications..................................................... 3
1.4 V-570 UV/VIS/NIR Spectrophotometer Specifications............................................. 4
2 General Description o Instrument ................................................................................ 7
2.1 V-530 Spectrophotometer Optical S stem ................................................................. 7
2.1.1 Opti al System................................................................................................................. 7
2.1.2 Ele tri al System............................................................................................................. 7
2.2 V-550/560/570 Spectrophotometers Optical S stem................................................ 9
2.2.1 Opti al System................................................................................................................. 9
2.2.2 Ele tri al System........................................................................................................... 11
3 Nomenclature and Functions o Components .........................................................12
3.1 V-530 Spectrophotometer: Overview ......................................................................... 12
3.1.1 Overview......................................................................................................................... 12
3.1.2 Rear Panel ..................................................................................................................... 13
3.2 V-550/560/570 Spectrophotometers: Overview ....................................................... 14
3.2.1 Overview......................................................................................................................... 14
3.2.2 Rear Panel ..................................................................................................................... 15
3.3 Standard Sample Chamber........................................................................................... 16
3.4 Intelligent Remote Module ............................................................................................ 17
4 Maintenance.......................................................................................................................18
4.1 Cautions for Routine Operation .................................................................................. 18
4.2 Cleaning the Sample Chamber .................................................................................... 18
4.3 Performance Check: Data Station T pe .................................................................... 19
4.3.1 Baseline (Abs 0 line) Flatness..................................................................................... 19
4.3.2 Wavelength Repeatability ............................................................................................ 20
4.3.3 Wavelength A ura y................................................................................................... 22
4.4 Performance Check: Intelligent Remote Module T pe.......................................... 24

x
4.4.1 Baseline (Abs 0 line) Flatness..................................................................................... 24
4.4.2 Wavelength Repeatability ............................................................................................ 25
4.4.3 Wavelength A ura y................................................................................................... 26
4.5 Replacement of Consumables .......................................................................................... 27
4.5.1 Repla ement of Fuse...................................................................................................... 27
4.5.2 Lamp Repla ement and Adjusting the Light Sour e Mirror .................................... 28
5 Troubleshooting ...............................................................................................................33

1
1 Spe ifi ations
The spe ifi ations of ea h model overed in this manual are given below. Refer to the
Operations manual for software spe ifi ations.
1.1 V-530 UV/VIS Spe trophotometer Spe ifi ations
Opti al system Single mono hromator
UV/VIS region: 1200 lines/mm on ave grating
Rowland off- ir le arrangement
Double beam type
Resolution 2 nm
Light sour e Deuterium lamp: 190 to 350 nm
Halogen lamp: 330 to 1100 nm
Light sour e hangeover
wavelength
Any wavelength between 330 and 350 nm an
be sele ted.
Wavelength range 190 to 1100 nm
Wavelength repeatability ±0.1 nm
Wavelength a ura y ±0.3 nm
Spe tral bandwidth 2 nm fixed
Wavelength display In in rements of 0.1 nm
Photometri mode Abs, %T. %R
Photometri range -2.000 to 3.000 Abs (0 to 200 %T)
Photometri display ±10000 %T
-2 to 5 Abs
Four digits and (-)symbol
Photometri repeatability ±0.001 Abs (0 to 0.5 Abs)
±0.002 Abs (0.5 to 1 Abs)
Photometri a ura y ±0.002 Abs(0 to 0.5 Abs)
±0.004 Abs(0.5 to 1 Abs)
±0.3 %T
Note: Tested with NIST SRM 930D
Response Qui k, Fast, Medium, Slow
Number of integrations 2, 8, 32, 128 times (1 datum/30mse )
Stray light 0.04 % (220 nm: NaI 10g/L aqueous solution;
340 nm: NaNO
2
50g/L aqueous solution)
Wavelength s anning 40, 100, 200, 400, 1000, 2000, 4000 nm/min
Wavelength moving speed 8000 nm/min
Data pit h 0.1, 0.2, 0.5, 1, 2, 5, 10 nm/data
(spe trum measurement)
0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 se /data
(time ourse measurement)
Baseline stability ±0.001 Abs/hour
(value obtained more than one hour after
turning ON the power when temperature
variation is within 5°C, wavelength: 250 nm,
and response: Slow.)

2
Baseline flatness ±0.001 Abs
(value obtained after baseline orre tion when
temperature variation is within 5°C,
wavelength: 200 to 1100 nm, response:
Medium, and wavelength s anning: 100
nm/min.)
Dete tor Sili on photodiode (S1337)
Power requirements 100, 115, 200, 220, 230, 240 V ±10 %, 130 W
Dimensions and weight 484(W)×435(D)×205(H) mm (ex luding
protrusions)
16 kg
Note: Photometric repeata ility, photometric accuracy and aseline flatness are the
values o tained more than one hour after the “Power” switch is turned ON.
1.2 V-550 UV/VIS Spe trophotometer Spe ifi ations
Opti al system Single mono hromator
UV/VIS region: 1200 lines/mm plane grating
Czerny-Turner mount
Double beam type
Resolution 0.1 nm
Light sour e Deuterium lamp: 190 to 350 nm
Halogen lamp: 330 to 900 nm
Light sour e hangeover
wavelength
Any wavelength between 330 and 350 nm an
be sele ted.
Wavelength range 190 to 900 nm
Wavelength repeatability ±0.1 nm (at a spe tral bandwidth of 0.5 nm)
Wavelength a ura y ±0.3 nm (at a spe tral bandwidth of 0.5 nm)
Spe tral bandwidth 0.1, 0.2, 0.5, 1, 2, 5, 10 nm
L2, L5, L10 nm (low stray-light mode)
Wavelength display In in rements of 0.1 nm
Photometri mode Abs, %T. %R, Sample, Referen e
Photometri range -2 to 3 Abs
Photometri display 0.0 to 200.0 %T
-2 to 5 Abs
Four digits and (-) symbol
Displays up to 000.0 %T and 0.000 Abs
Photometri repeatability ±0.001 Abs (0 to 0.5 Abs)
±0.002 Abs (0.5 to 1 Abs)
Photometri a ura y ±0.002 Abs (0 to 0.5 Abs)
±0.004 Abs (0.5 to 1 Abs)
±0.3 %T
Note: Tested with NIST SRM 930D
Response Qui k, Fast, Medium, Slow
Stray light 0.015 % (220 nm: NaI 10g/L aqueous solution;
340 nm: NaNO
2
50g/L aqueous solution)

3
Wavelength s anning 10, 20, 40, 100, 200, 400, 1000, 2000, 4000
nm/min
Wavelength moving speed 8000 nm/min
Data pit h 0.025, 0.05, 0.1, 0.2, 0.5, 1, 2, 5,10 nm/data
(spe trum measurement)
0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 se /data
(time ourse measurement)
Baseline stability ±0.0004 Abs/hour
(value obtained more than one hour after
turning ON the power when temperature
variation is within 5°C
, wavelength: 250 nm,
response: Slow, and spe tral bandwidth: 2nm.)
Baseline flatness ±0.001 Abs
(value obtained after baseline orre tion when
temperature variation is within 5°C,
wavelength: 200 to 850 nm, response: Medium,
spe tral bandwidth: 2 nm, and wavelength
s anning: 100 nm/min)
Dete tor Photomultiplier tube
Power requirements 100, 115, 200, 220, 230, 240 V ±10 %, 130 W
Dimensions and weight 460(W)×595(D)×260(H)mm (ex luding
protrusions)
30.5 kg
Note: Photometric repeata ility, photometric accuracy and aseline flatness are the
values o tained more than one hour after the “Power” switch is turned ON.
1.3 V-560 UV/VIS Spe trophotometer Spe ifi ations
Opti al system Double mono hromator
UV/VIS region: 600 lines/mm plane grating
Czerny-Turner mount
Double beam type
Resolution 0.1 nm
Light sour e Deuterium lamp: 190 to 350 nm
Halogen lamp: 330 to 900 nm
Light sour e hangeover
wavelength
Any wavelength between 330 and 350 nm an
be sele ted.
Wavelength range 190 to 900 nm
Wavelength repeatability ±0.1 nm (at a spe tral bandwidth of 0.5 nm)
Wavelength a ura y ±0.3 nm (at a spe tral bandwidth of 0.5 nm)
Spe tral bandwidth 0.1, 0.2, 0.5, 1, 2, 5, 10 nm
L2, L5, L10 nm (low stray-light mode)
Wavelength display In in rements of 0.1 nm
Photometri mode Abs, %T. %R, Sample, Referen e
Photometri range -2 to 5 Abs
Photometri display 0.0 to 200.0 %T
-2 to 5 Abs

4
Four digits and (-) symbol
Displays up to 000.0 %T and 0.000 Abs
Photometri repeatability ±0.001 Abs (0 to 0.5 Abs)
±0.002 Abs (0.5 to 1 Abs)
Photometri a ura y ±0.002 Abs (0 to 0.5 Abs)
±0.004 Abs (0.5 to 1 Abs)
±0.3 %T
Note: Tested with NIST SRM 930D
Response Qui k, Fast, Medium, Slow
Stray light 0.0003 % (220 nm: NaI 10g/L aqueous solution;
340 nm: NaNO
2
50g/L aqueous solution)
Wavelength s anning speed 10, 20, 40, 100, 200, 400, 1000, 2000, 4000
nm/min
Wavelength moving speed 8000 nm/min
Data pit h 0.025, 0.05, 0.1, 0.2, 0.5, 1, 2, 5,10 nm/data
(spe trum measurement)
0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 se /data
(time ourse measurement)
Baseline stability ±0.0004 Abs/hour
(value obtained more than one hour after
turning ON the power when temperature
variation is within 5°C
, wavelength: 250 nm,
response: Slow, and spe tral bandwidth: 2nm)
Baseline flatness ±0.001 Abs
(value obtained after baseline orre tion when
temperature variation is within 5°C,
wavelength: 200 to 850 nm, response: Medium,
spe tral bandwidth: 2 nm, and wavelength
s anning speed: 100 nm/min)
Dete tor Photomultiplier tube
Power requirements 100, 115, 200, 220, 230, 240 V ±10 %, 130 W
Dimensions and weight 460(W)×595(D)×260(H)mm (ex luding
protrusions)
32 kg
Note: Photometric repeata ility, photometric accuracy and aseline flatness are the
values o tained more than one hour after the “Power” switch is turned ON.
1.4 V-570 UV/VIS/NIR Spe trophotometer Spe ifi ations
Opti al system Single mono hromator
UV/VIS region: 1200 lines/mm plane grating
NIR region: 300 lines/mm plane grating
Czerny-Turner mount
Double beam type
Resolution 0.1 nm (UV/VIS region)
0.5 nm (NIR region)

5
Light sour e Deuterium lamp: 190 to 350 nm
Halogen lamp: 330 to 2500 nm
Light sour e hangeover
wavelength
Any wavelength between 330 and 350 nm an
be sele ted.
Dete tor hangeover
wavelength
Any wavelength between 750 and 900 nm an
be sele ted.
Wavelength range 190 to 2500 nm
Wavelength repeatability
UV/VIS region: ±0.1 nm (at a spe tral bandwidth of 0.5 nm)
NIR region: ±0.4 nm (at a spe tral bandwidth of 2.0 nm)
Wavelength a ura y
UV/VIS region: ±0.3 nm (at a spe tral bandwidth of 0.5 nm)
NIR region: ±1.5 nm (at a spe tral bandwidth of 2.0 nm)
Spe tral bandwidth 0.1, 0.2, 0.5, 1, 2, 5, 10 nm (UV/VIS region)
L2, L5, L10 nm (low stray-light mode)
0.4, 0.8, 1, 2, 4, 8, 20, 40 nm (NIR region)
Wavelength display In in rements of 0.1 nm
Photometri mode Abs, %T. %R, Sample, Referen e
Photometri range -2 to 3 Abs (UV/VIS region)
-0.3 to 3 Abs (NIR region)
Photometri display 0.0 to 200.0 %T
-2 to 5 Abs
Four digits and (-) symbol
Displays up to 000.0 %T and 0.000 Abs
Photometri repeatability ±0.001 Abs (0 to 0.5 Abs)
±0.002 Abs (0.5 to 1 Abs)
Photometri a ura y ±0.002 Abs (0 to 0.5 Abs)
±0.004 Abs (0.5 to 1 Abs)
±0.3 %T
Note: Tested with NIST SRM 930D
Response Qui k, Fast, Medium, Slow
Stray light
UV/VIS region: 0.015% (220 nm: NaI 10g/L aqueous solution;
340 nm: NaNO
2
50g/L aqueous solution)
NIR region: 0.1% (1690 nm: CH
2
Br
2
10 mm ell used)
Wavelength s anning speed 10, 20, 40, 100, 200, 400, 1000, 2000, 4000
nm/min
Wavelength moving speed 8000 nm/min(UV/VIS region)
32000 nm/min(NIR region)
Data pit h 0.025, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10 nm/data
(UV/VIR spe trum measurement)
0.1, 0.2, 0.5, 1, 2 , 5, 10 nm/data
(NIR spe trum measurement)
0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 se /data
(time ourse measurement)
Baseline stability ±0.0004 Abs/hour
(value obtained more than one hour after
turning ON the power when temperature
variation is within 5°
C, wavelength: 250 nm,

6
response: Slow, and spe tral bandwidth: 2nm)
Baseline flatness ±0.001 Abs
(value obtained after baseline orre tion when
temperature variation is within 5°C,
wavelength: 250 to 850 nm, response: Medium,
spe tral bandwidth: 2 nm, and wavelength
s anning speed: 400 nm/min, wavelength: 850
to 2500 nm, spe tral bandwidth: 8 nm,)
Dete tor Photomultiplier tube
Pbs photo ondu tive ell
Power requirements 100, 115, 200, 220, 230, 240 V ±10 %, 180 W
Dimensions and weight
Main unit 460(W)×595(D)×260(H) mm (ex luding
protrusions)
33 kg
Pbs power supply unit 100(W)×170(D)×125(H) mm (ex luding
protrusions)
1.9 kg
Note: Photometric repeata ility, photometric accuracy and aseline flatness are the
values o tained more than one hour after the “Power” switch is turned ON.

7
2 General Des ription of Instrument
2.1 V-530 Spe trophotometer Opti al System
2.1.1 Opti al System
The V-530 is designed to measure the absorption spe trum of a sample at
wavelengths in the range 190 to 1100 nm. The light sour es used in the V-530 are a
deuterium(D
2
) lamp (190 to 350 nm) for the UV region and a halogen (WI) lamp (340 to
1100 nm) for the VIS/NIR region. The light from the light sour e is onverged and
enters the mono hromator. The light is dispersed by the grating in the mono hromator
and onverges onto the exit slit. The light that has passed through the exit slit is
mono hromated. This light is split into two beams, one going to the sample to be
measured and the other to the referen e sample su h as a solvent. The light that has
passed through the sample and the referen e sample is alternately in ident upon the
sili on photodiode.
D
D
Ref
Sam
F
G
WI
M
5
M
2
M
(BS)
3
S
2
S
1
D
2
M
1
M
4
WI
D
2
S
F
G
BS
D
Sam
Ref
Deuterium lamp
halogen lamp
Slit
Filter
Grating
Bean splitter
Dete tor
Sample beam
Referen e beam
Figure 2.1 V-530 Opti al system
2.1.2 Ele tri al System
The onfiguration of the ele tri al system used in the V-530 is shown in Fig 2.2. The
light in ident upon the sili on photodiode is onverted into an ele tri al signal and,
after being subje ted to syn hronous re tifi ation, is onverted into a digital signal and
enters the mi ro omputer. The signal pro essed by the mi ro omputer is displayed on
the display unit as digital data or a spe trum.
A tions su h as light sour e hangeover, wavelength drive, filter drive, et . are
ontrolled by the mi ro omputer.

8
D2 lamp
CPU, ROM, RAM, I/O
D/A
A/D
Mirror
Filter
Mono hromator
Limiter
WI lamp
Serial 1
Serial 2
A essory input S essory output
Coarse
Adj.
Fine
Adj.
Dete tor Amp.
Battery
Main unit
320×240
10×6 キー
CPU
ROM, RAM
I/O
I/O
LCD
ROM Card
RAM Card
Autozero Goto WL File
Start Stop Print
iRM
Tou h key
LCD
Cintroller
Battery
Centroni s
Serial 1
Power supply
Figure 2.2 V-530 Ele tri al system

9
2.2 V-550/560/570 Spe trophotometers Opti al System
2.2.1 Opti al System
Figures 2.3 through 2.5 show the opti al systems of the V-550, V-560 and V–570
spe trophotometers, respe tively. The V-550 and V-560 measure the absorption
spe trum of a sample at wavelengths in the range 190 to 900 nm and the V-570
measures the absorption spe trum of a sample at wavelengths in the range 190 to
2500 nm. In ea h model, the light sour es used are a deuterium (D
2
) lamp (190 to 350
nm) for use in the ultraviolet region and Halogen (WI) lamp (340 to 2500 nm) for use in
the VIS/NIR region. The light from the light sour e is onverged and enters the
mono hromator. It is dispersed by the grating in the mono hromator and the light
passing through the exit slit is mono hromated. This light is split into two light paths by
a se tor mirror, one in ident on the sample to be measured and the other on the
referen e sample su h as a solvent. The light that has passed through the sample and
referen e sample is in ident on the photomultiplier tube or Pbs photo ondu tive ell.
Figure 2.3 V-550 Opti al system
WI, D
2
: Light sour e
M : Mirror
S : Slit
F : Filter
G : Grating
M8 : beam splitter
PM : Dete tor
Sam : Sample beam
Ref : Referen e beam
W : window