Knauer Azura DAD 6.1L User manual

Detector DAD 6.1L, DAD 2.1L, MWD 2.1L
User Manual
Detektor DAD 6.1L, DAD 2.1L, MWD 2.1L
Benutzerhandbuch
V6700
HPLC

3
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Table of Contents
Note For your own safety, read the manual and always observe the
warnings and safety information on the device and in the 
manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Definition of Personal and Material Damage . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Contact Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installation Site and Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Operating Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Inserting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Inserting the Flow Cell with Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Connecting the Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Using PEEK Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Connecting the Device to the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . 25
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . 25
Pin Header Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Wiring the Pin Header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Integrator Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Integrating the Detector into a System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

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KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Switch-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operation with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . 32
Control with Control Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Control with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Re-Setting the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Optimizing the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Warm-Up Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Selecting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Flow Cell Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Path Length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Pressure Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Remote Operation (Fiber Optics) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Selecting the Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Bandwidth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Spectral Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Time Constant and Data Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Integration time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Subtraction of the Baseline Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . 41
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Installation Qualification (IQ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Operation Qualification (OQ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Possible Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Contact the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Putting the Device out of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Disconnect from Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Cleaning the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Rinsing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

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KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Replacing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Replacing the Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Removing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . 53
Installing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . 55
Removing a Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
DAD 6.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
DAD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
MWD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Conditions for Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Legal Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Flow Cells with Fiber Optics Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Table of Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
To whom it may concern In case you prefer a French language user manual for this prod-
uct, submit your request including the corresponding serial
number via email or fax to KNAUER:
[email protected]
[phone]
Thank you.
A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit
contacter KNAUER par email ou par fax avec le no. de série:
[email protected]
[phone]
Merci beaucoup.

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
6IntendedUse
Intended Use
Note Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equip-
ment of the device could fail.
Description The detector is suitable for analytical, semipreparative, and pre-
parative applications.
Scope of application The device can be used in the following areas:
Biochemistry analysis
Chemical analysis
Food analysis
Pharmaceutical analysis
Environmental analysis
Device Overview
Front View
On the front of the device, you find the flow cell and a lamp
cover for replacing the lamp.
Rear View
On the back of the detector, the connectors for LAN and power
plug are located as well as the serial number of the device and
the power switch (on/off).
RS-232, service interface Please note that the service interface is solely used for repair and
maintenance tasks performed by service technicians.
Legend
1Leak tray
2Capillary guide
3Lamp cover
4Serial number
5Leak Management
6Switch/Standby button
7Status LED
8Flow cell
Fig. 1 Front view (example DAD 6.1L)
8
7
2
3
2
2
4
1
6
5

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
7IntendedUse
Side View
DAD 6.1L On the side of the detector, you find the connector for the oper-
ating panel Control Unit (see See “Control with Mobile Control”
on page 32.).
Note The USB ports of the DAD 2.1L and the MWD 2.1L are not
active. These detectors cannot be operated with the Control
Unit.
Features
In an HPLC system, the detector serves to detect substances in
liquids and show their concentration. The sensitivity of the
detector depends on the installed flow cell.
Optical system The detectors measure the light absorption of the sample in the
ultraviolet and visual spectra. The entire spectrum permeates the
sample and afterwards is split up by a grate. The split light falls
onto a geometric array of 1024 (DAD 6.1L) or 256 (DAD 2.1L,
MWD 2.1L) separate photodiodes – the photodiode array.
Legend
1Device information
2Integrator outlets
3LAN connector
4Service Interface
5Pin header
6Fan
7Connection and
power switch
Fig. 2 Rear view (example DAD 6.1L)
2345 671
Legend
1USB port for the
Control Unit
Fig. 3 USB port for the Control Unit
1

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
8IntendedUse
The optical system of the AZURA DAD 6.1L detector is depicted
in figure 4. An active temperature control with 0.5°C sensitivity
ensures baseline stability for minimal baseline drift.
Lamps The lamps can be exchanged quickly and easily without the
need of a service technician.
DAD 6.1L Two light sources, a deuterium lamp and a halogen lamp, cover
the entire UV-Vis wavelength range of remove space 
190–1020 nm.
DAD 2.1L, MWD 2.1L A deuterium lamp covers the UV wavelength range of 190–500
nm.
Lamp mirror
DAD 6.1L
Maximum light intensity is provided by effectively mixing the
light sources from the deuterium and halogen lamps with a mir-
ror using Polka Dot technology.
Flow cell Various types of flow cells are available to the user and allow a
variety of applications. Flow cell cartridges with standard and
KNAUER LightGuide technology are available.
KNAUER LightGuide
flow cell cartridge
KNAUER LightGuide flow cell cartridges combine a maximum
light transmission (using total reflection) with a minimum cell
volume to offer an ideal signal-to-noise ratio.
Adapter for fiber optics are additionally available. Fiber optics
allow separating the flow cell from the detector. This way, work-
ing with dangerous, explosive, or toxic materials becomes safer.
Data rate The detector measures with a maximum data rate of 100 Hz.
During the measurement, approx. 100 spectra per second are
recorded.
Leak Management Integrated leak management drains liquids resulting from leaks.
Legend
1Deuterium lamp
2Halogen lamp
3Flow cell
4Diode array sensor
5Diffraction grating
6Mirror
Fig. 4 Optical system DAD 6.1L
3
5
1
2
4
6

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
9IntendedUse
GLP data The Mobile Control and the different software products (e.g.
ClarityChrom®or OpenLAB®) can be used to display or read out
the GLP data (e.g. operating hours, lamp operating hours, num-
ber of lamp ignitions, etc.) of the detector. For the DAD 6.1L,
the Control Unit is available for reading out the GLP data. You
find a detailed description on how to display or read out GLP
data in the respective user manuals.
Optional accessories The following optional accessories are available:
The Mobile Control app for control on Android and
Windows 8
The Control Unit for operating the DAD 6.1L
Solvents
Even small quantities of other substances, such as additives,
modifiers, or salts can influence the durability of the materials.
If there is any doubt, contact the Technical Support of the man-
ufacturer.
Suitable solvents
Acetate buffer solutions
Acetone at 4 °C–25 °C (39.2 °F–77.0 °F)1
Acetonitrile2
Benzene
Carbon dioxide (liquid 99.999 % CO2)
Chloroform
Dilute acetic acid (e.g. 0.1–1 %) at 25 °C/77.0 °F
Dilute ammonia solution
Dilute sodium hydroxide (1 M)
Ethyl acetate
Ethanol
Formiate buffer solution
Isopropanol
Methanol
Phosphate buffer solutions (0.5 M)
Toluol
Water
1. valid for the specified temperature range
2. not recommended in combination with PEEK small parts and PEEK capillary

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
10 Intended Use
Less suitable solvents
Diethylamine (0.1 %) (DEA)
Dilute phosphoric acid
Dimethyl sulfoxide (DMSO)
Methylene chloride1
Slightly volatile solvents
Tetrahydrofuran (THF)1
Triethylamine (0.1 %) (TEA)
Trifluoroacetic acid (0.1 %) (TFA)
1. not recommended in combination with PEEK small parts and PEEK capillaries
Not suitable solvents
Concentrated mineral and organic acids
Concentrated bases
Halogenated hydrocarbons, e.g. Freon®
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles
Solvents not suitable for degassers
Azides
Benzene
Carbon dioxide (liquid 99.999 % CO2)
Concentrated mineral and organic acids
Concentrated bases
Dilute sodium hydroxide (1 M)
Halogenated hydrocarbons, e.g. Freon®
Hexafluoroisopropanol
Hexanes (60 % n-Hexane)
Hydro fluoro solvents
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
11 Scope of Delivery
Scope of Delivery
Note Only use spare parts and accessories made by KNAUER or a
company authorized by KNAUER.
DAD 6.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kit DAD 6.1L
AZURA
DAD 2.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kit DAD 2.1L
AZURA
MWD 2.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kit MWD 2.1L
AZURA

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
12 Safety for Users
Safety for Users
Professional group The user manual addresses persons who are qualified as chemi-
cal laboratory technicians or have completed comparable voca-
tional training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a
limited extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by
the company KNAUER or an authorized company.
If you do not belong to this or a comparable professional group,
you may not perform the work described in this user manual
under any circumstances. In this case, please contact your supe-
rior.
Safety equipment When working with the device, take measures according to lab
regulations and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifica-
tions in the user manual
National and international regulations pertaining to labora-
tory work
Original spare parts, tools, and solvents made or recom-
mended by KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident
insurance companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other
areas of the HPLC system.
Only use a given PEEK fitting for one specific port and never
re-use it for other ports. Always install new PEEK fittings on
each separate port.
Follow KNAUER or manufacturer's instructions on caring for
the columns

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
13 Safety for Users
More safety-relevant information is listed in alphabetical order in
the following table:
Where is use of the
device prohibited?
Never use the system in potentially explosive atmospheres with-
out appropriate protective equipment. For further information,
contact the Technical Support of KNAUER.
Taking the device out of
operation
At any time, take the device completely out of operation by
either switching off the power switch or by pulling the power
plug.
Opening the device The device may be opened by the KNAUER Technical Support or
any company authorized by KNAUER only.
Topic Explanations
Flammability Organic solvents are highly flammable.
Since capillaries can detach from their
screw fittings and allow solvent to escape,
it is prohibited to have any open flames
near the analytical system.
Solvent tray Risk of electrical shock or short circuit if
liquids get into the device's interior. For
this reason, place all bottles in a solvent
tray.
Solvent lines Install capillaries and tubing in such a way
that liquids cannot get into the interior in
case of a leak.
Leaks Regularly check if any system compo-
nents are leaking.
Power cable Defective power cables are not to be used
to connect the device and the power sup-
ply system.
Self-ignition
point
Only use eluents that have a self-ignition
point higher than 150 °C under normal
ambient conditions.
Power strip If several devices are connected to one
power strip, always consider the maxi-
mum power consumption of each device.
Power supply Only connect devices to voltage sources,
whose voltage equals the device's volt-
age.
Toxicity Organic eluents are toxic above a certain
concentration. Ensure that work areas are
always well-ventilated! Wear protective
gloves and safety glasses when working
on the device!

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
14 Safety for Users
Definition of Personal and Material
Damage
Possible dangers related to the device are divided into personal
and material damage in this user manual.
Decontamination
Contamination of devices with toxic, infectious or radioactive
substances poses a hazard for all persons during operation,
repair, sale, and disposal of a device.
DANGER! 
Danger if getting in contact with toxic, infectious
or radio-active substances. 
Before disposing off or sending away contami-
nated devices, commission an expert with the
decontamination.
All contaminated devices must be properly decontaminated by a
specialist company or the operating company before they can
be recommissioned, repaired, sold, or disposed of.
All materials or fluids used for decontamination must be col-
lected separately and disposed of properly.
Category Explanations
DANGER! Lethal or very serious injuries can occur.
WARNING! Serious or moderate injuries can occur.
CAUTION! Moderate injuries can occur. Device defects
can occur.

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
15 Symbols and Signs
Symbols and Signs
The following symbols and signs can be found on the device, in
the chromatography software or in the user manual:
Symbol Meaning
Warning signs High-voltage hazard
Electric shock hazard
Electrostatic discharge hazard, damages
to system, device, or components may
occur.
General warning sign, moderate injuries
may occur and also damages to system,
device, or components.
UV-light hazard, eye injuries may occur.
Burn hazard, hot surface
Hazardous substances.
Obey maximum load for leak tray during
transportation, installation and operation.
Mandatory signs Wear protective gloves to avoid skin irrita-
tions.
CE mark A device or system marked with CE fulfills
the product specific requirements of Euro-
pean directives. This is confirmed in a
Declaration of Conformity.
Testing seals in Canada and the USA at
nationally recognized testing centers
(NRTL). The certified device or system has
successfully passed the quality and secu-
rity tests.

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
16 Installation
Installation
This chapter describes all preparatory steps prior to the start-up.
If you encounter difficulties during installation, contact the
Technical Support.
Contact Data
Transport
Carefully prepare the device for transport or storage. If you want
to return your device to KNAUER for repairs, enclose the Service
Request Form which can be downloaded from our website.
Device data For a secure transport, note the weight and dimensions of the
device (see Technical Data).
CAUTION! 
Damage to the device by lifting on protruding
housing parts! 
Lift the device on the side of the housing only.
Lifiting Clasp the device at its side panels and lift it out of the packag-
ing. Do not hold onto front cover or leak tray.
Only if the requirements for ambient conditions of the operating
environment are met, can the intended use be ensured. You will
find the ambient conditions under Technical Data.
CAUTION! 
Defect of the device due to overheating possible! 
Protect the device against exposure to direct sun-
light. 
Keep clear at least 15 cm at the rear and 5–10 cm
at each side for air circulation.
Installation Site and Space Requirements
Position the device on a level and even surface.
Sunlight: Protect the device against direct exposure to sun-
light.
AC system: Set up the device at a location not exposed to air
drafts.
Vibration: Do not set up the device in the vicinity of other
machines that cause floor vibrations.
Technical Support Phone [phone]
Fax [phone]
E-mail suppo[email protected]

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
17 Installation
Lateral distance to other devices:
At least 5 cm if another device is set up on one side
At least 10 cm if further devices are set up on both sides
Leave the power plug on the rear of the device accessible
to be able to disconnect the device from the mains.
Avoid sources of high frequencies near the device. High-fre-
quency sources may compromise measuring values.
Operating Environment
Earth quake regions If the device is set up in a region where earth quakes are com-
mon, secure the device at the two fixing points 1. The fixing
points are situated on both sides of the device.
Space Requirements
Side clearance to other devices:
At least 5 cm, if there is another device on one side.
At least 10 cm, if there are devices set up on both sides.
At least 15 cm on the rear panel for the fan.
Setup
Prior to setting up the device, find a suitable place according to
the requirements and remove the packaging. The requirements
and a description can be found in the following section.
Unpacking
Prerequisite Check packaging for damage caused during transportation.
Tool Utility knife
CAUTION! 
Damage to the device by lifting on protruding
housing parts! 
Lift the device on the side of the housing only.
Fig. 5 Fixing points on the device
1

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
18 Installation
Next steps Store packaging and keep the included packing list for repeat
orders.
Note The test cell is used during operation qualifications and must be
stored.
Inserting the Flow Cell
Prerequisite No flow cell or test cell is installed.
The device is switched off.
Procedure Insert the flow cell.
Note Pay attention to the compatibility of the flow cells.
WARNING! 
Irritation of retina through UV light! Concen-
trated UV light can leak out from the flow cell or
the fiber optic connectors.
Switch off the device or the lamps.
Next steps Connect the capillaries.
Process
1. Set-up the package in such a way that you can read the
label. Using the utility knife, cut the adhesive tape and
open the packaging.
2. Remove the foam insert. Take out the accessory kit and the
manual.
3. Open the accessory kit and check the scope of delivery. In
case any parts are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging
and place it on its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during
transport. In case you notice any damage, contact the
Technical Support.
6. Place the device in its site of operation and remove protec-
tive foil.
Procedure Process Figure
1. Place the flow cell into
the opening and slide
to the back until it
clicks into place inside
the device.
Fig. 6 Inserting the Flow Cell

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
19 Installation
Inserting the Flow Cell with Fiber Optics
You may also connect the flow cell with fiber optic cables. The
fiber optics are connected via an adapter.
UV light will cause the fiber optics to become blind with time
(solarization), making them no longer suitable for use.
Observe the following regarding the use of UV fiber optics:
Do not touch the ends of the fiber optics with your fingers, as
this could falsify the measurement.
Handle the fiber optics with care, avoid impacts or hard
actions.
Move the fiber optics carefully without using pressure or
bending it.
Prerequisites No flow cell or test cell is installed.
Auxiliary material Screw-type cap fittings for the fiber optics
WARNING! 
Irritation of retina through UV light! Concen-
trated UV light can leak out from the flow cell or
the fiber optic connectors.
Switch off the device or the lamps.
Next steps Connect the capillaries.
Procedure Process Figure
1. Remove the cap fittings
1from the fiber optics
adapter.
2. Place the fiber optics
adapter into the open-
ing and slide to the
back until it clicks into
place inside the device.
Fig. 7 Fiber optics adapter
3. Remove the screw-type
cap fittings 1from the
fiber optics.
4. Manually, screw the
fiber optics 2to the
fiber optics adapter.
Fig. 8 Screw-type cap fitting
on the fiber optics
5. Remove the cap fittings
from the flow cell.
6. Manually, screw the fit-
tings3of the fiber
optics to the flow cell.
Fig. 9 Fitting of the fiber
optics on the flow cell
1
1
1
3

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
20 Installation
Connecting the Capillaries
Capillaries connect the detector with the column and guides li-
quids. For the flow cell connectors, we recommend PEEK capil-
laries and PEEK connectors.
CAUTION! 
Damage to the PEEK threads of the flow cell
through metal fittings possible.
Attach capillaries with PEEK fittings only.
Observe torque of 0.5 Nm maximum.
Prerequisite The flow cell is inserted.
Screw-type cap fittings and cap fittings are removed.
Procedure Attach the capillaries with screw fitting.
Next steps Bring the device into operation.
Using PEEK Fittings
PEEK fittings withstand a maximum pressure of 400 bar. Note
the torque of 0.5 Nm.
Procedure Process Figure
1. Push the capillary 1
through the fitting 2.
2. Slide the lock ring 3
over the tubing. Note
that the tapered end of
the lock ring has to
point to the seal
ring 4.
3. Attach the seal ring 4.
Fig. 10 Capillary connectors
4. Fasten the capillary at
the flow cell 4finger-
tight.
Fig. 11 Flow cell with capillary
1324
13
24
4
4

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
21 Installation
Connecting the Leak Management
The leak management consists of the leak sensor and the drain-
age system. The drainage system ensures that escaping liquids
flow into a waste bottle. If there is too much liquid, the red LED
starts flashing. Both the device and the data acquisition via chro-
matography software are stopped.
Prerequisite The front cover has been removed.
Procedure Process Figure
1. Carefully push the fun-
nel 1into the center
opening of the capillary
guide 2.
Fig. 12 Funnel and capillary
guide
2. Push the long ending of
the first nozzle 4into
the hose 3.
Fig. 13 Hose and nozzle
3. Connect the nozzle and
the funnel.
4. Push the other end of
the hose onto the noz-
zle 5of the leak tray.
Fig. 14 Hose connected to
device
12
3
4
5