Knauer Azura DAD 6.1L User manual

Detector DAD 6.1L, DAD 2.1L, MWD 2.1L
User Manual
Detektor DAD 6.1L, DAD 2.1L, MWD 2.1L
Benutzerhandbuch
V6700
HPLC

3
KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Table of Contents
Note For your own safety, read the manual and always observe the
warnings and safety information on the device and in the 
manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Definition of Personal and Material Damage . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Contact Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installation Site and Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Operating Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Space Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Inserting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Inserting the Flow Cell with Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Connecting the Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Using PEEK Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Connecting the Device to the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . 25
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . 25
Pin Header Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Wiring the Pin Header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Integrator Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Integrating the Detector into a System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

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KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Switch-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operation with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . 32
Control with Control Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Control with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Re-Setting the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Optimizing the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Warm-Up Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Selecting the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Flow Cell Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Path Length . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Pressure Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Remote Operation (Fiber Optics) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Selecting the Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Bandwidth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Spectral Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Time Constant and Data Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Integration time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Subtraction of the Baseline Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . 41
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Installation Qualification (IQ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Operation Qualification (OQ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Possible Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Contact the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Putting the Device out of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Disconnect from Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Cleaning the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Rinsing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

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KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
Replacing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Replacing the Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Removing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . 53
Installing the Deuterium or Halogen Lamp . . . . . . . . . . . . . . . . . . . . . . . . . 55
Removing a Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
DAD 6.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
DAD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
MWD 2.1L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Conditions for Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Legal Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Flow Cells with Fiber Optics Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Fiber Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Table of Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
To whom it may concern In case you prefer a French language user manual for this prod-
uct, submit your request including the corresponding serial
number via email or fax to KNAUER:
[email protected]
[phone]
Thank you.
A qui que ce soit Si jamais vous préfériez un manuel en francais pour ce poduit
contacter KNAUER par email ou par fax avec le no. de série:
[email protected]
[phone]
Merci beaucoup.

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
6IntendedUse
Intended Use
Note Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equip-
ment of the device could fail.
Description The detector is suitable for analytical, semipreparative, and pre-
parative applications.
Scope of application The device can be used in the following areas:
Biochemistry analysis
Chemical analysis
Food analysis
Pharmaceutical analysis
Environmental analysis
Device Overview
Front View
On the front of the device, you find the flow cell and a lamp
cover for replacing the lamp.
Rear View
On the back of the detector, the connectors for LAN and power
plug are located as well as the serial number of the device and
the power switch (on/off).
RS-232, service interface Please note that the service interface is solely used for repair and
maintenance tasks performed by service technicians.
Legend
1Leak tray
2Capillary guide
3Lamp cover
4Serial number
5Leak Management
6Switch/Standby button
7Status LED
8Flow cell
Fig. 1 Front view (example DAD 6.1L)
8
7
2
3
2
2
4
1
6
5

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
7IntendedUse
Side View
DAD 6.1L On the side of the detector, you find the connector for the oper-
ating panel Control Unit (see See “Control with Mobile Control”
on page 32.).
Note The USB ports of the DAD 2.1L and the MWD 2.1L are not
active. These detectors cannot be operated with the Control
Unit.
Features
In an HPLC system, the detector serves to detect substances in
liquids and show their concentration. The sensitivity of the
detector depends on the installed flow cell.
Optical system The detectors measure the light absorption of the sample in the
ultraviolet and visual spectra. The entire spectrum permeates the
sample and afterwards is split up by a grate. The split light falls
onto a geometric array of 1024 (DAD 6.1L) or 256 (DAD 2.1L,
MWD 2.1L) separate photodiodes – the photodiode array.
Legend
1Device information
2Integrator outlets
3LAN connector
4Service Interface
5Pin header
6Fan
7Connection and
power switch
Fig. 2 Rear view (example DAD 6.1L)
2345 671
Legend
1USB port for the
Control Unit
Fig. 3 USB port for the Control Unit
1

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
8IntendedUse
The optical system of the AZURA DAD 6.1L detector is depicted
in figure 4. An active temperature control with 0.5°C sensitivity
ensures baseline stability for minimal baseline drift.
Lamps The lamps can be exchanged quickly and easily without the
need of a service technician.
DAD 6.1L Two light sources, a deuterium lamp and a halogen lamp, cover
the entire UV-Vis wavelength range of remove space 
190–1020 nm.
DAD 2.1L, MWD 2.1L A deuterium lamp covers the UV wavelength range of 190–500
nm.
Lamp mirror
DAD 6.1L
Maximum light intensity is provided by effectively mixing the
light sources from the deuterium and halogen lamps with a mir-
ror using Polka Dot technology.
Flow cell Various types of flow cells are available to the user and allow a
variety of applications. Flow cell cartridges with standard and
KNAUER LightGuide technology are available.
KNAUER LightGuide
flow cell cartridge
KNAUER LightGuide flow cell cartridges combine a maximum
light transmission (using total reflection) with a minimum cell
volume to offer an ideal signal-to-noise ratio.
Adapter for fiber optics are additionally available. Fiber optics
allow separating the flow cell from the detector. This way, work-
ing with dangerous, explosive, or toxic materials becomes safer.
Data rate The detector measures with a maximum data rate of 100 Hz.
During the measurement, approx. 100 spectra per second are
recorded.
Leak Management Integrated leak management drains liquids resulting from leaks.
Legend
1Deuterium lamp
2Halogen lamp
3Flow cell
4Diode array sensor
5Diffraction grating
6Mirror
Fig. 4 Optical system DAD 6.1L
3
5
1
2
4
6

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
9IntendedUse
GLP data The Mobile Control and the different software products (e.g.
ClarityChrom®or OpenLAB®) can be used to display or read out
the GLP data (e.g. operating hours, lamp operating hours, num-
ber of lamp ignitions, etc.) of the detector. For the DAD 6.1L,
the Control Unit is available for reading out the GLP data. You
find a detailed description on how to display or read out GLP
data in the respective user manuals.
Optional accessories The following optional accessories are available:
The Mobile Control app for control on Android and
Windows 8
The Control Unit for operating the DAD 6.1L
Solvents
Even small quantities of other substances, such as additives,
modifiers, or salts can influence the durability of the materials.
If there is any doubt, contact the Technical Support of the man-
ufacturer.
Suitable solvents
Acetate buffer solutions
Acetone at 4 °C–25 °C (39.2 °F–77.0 °F)1
Acetonitrile2
Benzene
Carbon dioxide (liquid 99.999 % CO2)
Chloroform
Dilute acetic acid (e.g. 0.1–1 %) at 25 °C/77.0 °F
Dilute ammonia solution
Dilute sodium hydroxide (1 M)
Ethyl acetate
Ethanol
Formiate buffer solution
Isopropanol
Methanol
Phosphate buffer solutions (0.5 M)
Toluol
Water
1. valid for the specified temperature range
2. not recommended in combination with PEEK small parts and PEEK capillary

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
10 Intended Use
Less suitable solvents
Diethylamine (0.1 %) (DEA)
Dilute phosphoric acid
Dimethyl sulfoxide (DMSO)
Methylene chloride1
Slightly volatile solvents
Tetrahydrofuran (THF)1
Triethylamine (0.1 %) (TEA)
Trifluoroacetic acid (0.1 %) (TFA)
1. not recommended in combination with PEEK small parts and PEEK capillaries
Not suitable solvents
Concentrated mineral and organic acids
Concentrated bases
Halogenated hydrocarbons, e.g. Freon®
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles
Solvents not suitable for degassers
Azides
Benzene
Carbon dioxide (liquid 99.999 % CO2)
Concentrated mineral and organic acids
Concentrated bases
Dilute sodium hydroxide (1 M)
Halogenated hydrocarbons, e.g. Freon®
Hexafluoroisopropanol
Hexanes (60 % n-Hexane)
Hydro fluoro solvents
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles

KNAUER DAD 6.1L, DAD 2.1L, MWD 2.1L User Manual V6700, Version 1.0
11 Scope of Delivery
Scope of Delivery
Note Only use spare parts and accessories made by KNAUER or a
company authorized by KNAUER.
DAD 6.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kit DAD 6.1L
AZURA
DAD 2.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kit DAD 2.1L
AZURA
MWD 2.1L Detector
Power cable
User manual English/German
Installation Qualification documents
Accessory kit MWD 2.1L
AZURA