Kontron SFM25 User manual

KONTRON
INSTRUMENTS
SFM
Instruction manual

18 Instruction manual SFM
TABLE OF CONTENTS
0. GENERALINFORMATION
1. INSTALLATION
l . l Unpacking 2
1.2 Checking the Instrument ' s serialnumber 2
1.3 Checking the accessories 2
1.4 Checking the mains supply 3
1.5 Installation 4
1.5.1Installation ofthe xenon lamp 6
1.5.2 Lamp adjustment 7
1.5.3 Removal ofthe xenon lamp 8
1.5.4 Fitting the sample compartment accessories 9
1.5.5Adjustment ofthe HPLC flow cell 10
1.5.6Printer,Recorder 11
2. INSTRUMENT DESCRIPTION
2.1 Specifications 2
2.2 Construction ofthe Instrument 4
2 . 3 Keyboard 6
2.4 Display 8
2.5 Parameterplan 9
2.5.1 Wavelength control(group 2)11
2.5.2Datacontrol(group 3)12
2.5.3 Printer/Plotter (group 4)14
2.5.4Interfacecontrol RS-232C (group 5)16
3. OPERATION
3.1 Operatinghints 2
3.2 Initialisation 2
3 . 3 Programming 3
3.3.1 Generalremarks 3
3.3.2Wavelength programming 4
3.3.3Calibration 5
3.3.4Blank compensation 6
3.3.5 Calculation of average value 7
3.3.6Emission scan 8
3.3.7Exciationscan 10
3.3.8 Synchroscan 12
3.3.9Parameterviewing 14
3.3.10 Printout of parameter 15
3.3.11 AutomaticdataOutput Lambda-FIX 16
3.3.12 AutomaticdataOutput Lambda-SCAN 17
3.4 Measurement examples 18
3.4.1 Fixed wavelength 18
3.4.2Emissionscan 21
3.4.3Excitationscan 24
3.4.4Synchroscan 27
3.5 Changing the spectral bandwidth 30
3.6 Switching offthe Instrument 31
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Instruction manual SFM
TA
4.
4.1
6.
6.1
6.2
6.3
6.4
6.5
6.6
BLE OF CONTENTS (cont. )
ERROR DETECTION
Errormessages
OPTIONS
Thermostatted 4 cell changer
for 10x10 mm Standardcells 91-00431
HPLC flow cellwithholder 91-00430
Slit holderwithslit 5 and10nm 91-00432
Slit holderwithslit 10and20nm 91-00481
Filter holder for excitation and emission 91-00434
Softwareextensionpack (Instr. 84-00095)
Plotter/Printer and method files 91-00441
6.6.1 Storing a method
6.6.2Recall ofa method file
6.6.3Intallationinstruction
6.6.4Operation
6.6.5 P800 used äs plotter in TIMEDRIVE
6.6.6 P800 used äs plotter in LAMBDADRIVE
6.6.7 Howto produce an overlay onthe P800
6.6.8 Automaticcurveoverlaywith P800
2
2
2
3
4
5
6
8
10
11
(for SW Version 8603 and up)
6.6.9Baselinecorrection 13
(for SW Version 8603 andup)
6.7 Photomultiplier R446 (200-800 nm) 91-00433
6.8 Polarization kit (Instr. 84-00219)91-00493
7. APPENDIX
7.1 Opticaldiagram 2
7.2 Mechanicalconstruction 3
s^ 7.3 Blockdiagram 4
7.4 Softwareblock 5
7.5 Samplecells 6
7.6 Functioncontrol 7
7.7 Communication viaRS23211
7.7.1Format of data-out-string 11
7.7.2 Code Table - Interface RS23212
7.7.3 SFM25 entrymask 13
07.86 0 GENERALINFORMATION - 3 -

K3 Instruction manual SFM
1. INSTALLATION
l. l Unpacking 2
1.2 Checking the Instrument's serial number 2
1.3 Checking the accessories 2
1.4 Checking the mainssupply 3
1.5 Installation 4
1.5.1Installation ofthe xenon lamp 6
1.5.2 Lamp adjustment 7
1.5.3 Removal ofthe xenonlamp 8
1.5.4 Fitting the samplecompartmentaccessories 9
1.5.5 Adjustment ofthe HPLC flow cell 10
1.5.6 Printer,Recorder 11
09.84 l INSTALLATION
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Instruction manual
SFM
1.1 UNPACKING
Compare the consignment with- the- delivery note. If parts are missing or
damaged, please inform your local KONTRONrepresentativeimmediately.
Transport the Instrument only in its originalpacking box.
1.2 CHECKING THE INSTRUMENTS SERIAL NUMBER
The plate with the serialnumber is mounted onthe left side ofthe
Instrument.Thisnumber must be identicalwith the serialnumber onthe
delivery note.
1.3 CHECKING THE ACCESSORIES
The following Standardaccessories are included:
Number
of items
1
1
1
1
1
1
1
1
Description
Mains cable Schuco
Recorder connectioncable
4 cell changer(installed) or
HPLC-flow cell
Hex wrench size 3
Fuse set
Xenon lamp 150W
Instruction manual
InstallationReport
Order no.
55-65601
93-00325
89-00200
91-00430
40-30003
91-00435
54-01001
95-00161
84-00046
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Instruction manual
SFM
1.4 CHECKING THE MAINS SUPPLY
TheSFM25is factory setfor 220V 50/60HzL.- For.Operationwithother
mains voltages, the correctvoltagemust besetonthe voltageselector
(see picture below).
Possible voltages:100,120,
220und240VAC
The fuses onthe voltageselector (1)and XENON lamp (2) have tobe
adjustedaccording tothe mainssupply.
Frequency adjustment for 50/60Hz isnot necessary.
_7
WARNING
DISCQNNF.CT FROM MAINS BEFORE OPENiHQ
FUSES
VDLTAQE
HANGE
MOTECTXWfiEMlCE
war WPH TUE S*WE
TYPE AXQ RITWO
Unplug mains cable
Open the snap-cover (A) with a screw
driver atthetop
Pull marker wheel (B)and turn itto
the requiredposition
Push (B) backintoposition
Pull out fuseholders (C) insert the
correctfuse
Pushholders (C) backintoposition,
obey arrows
Close snap-cover (A)
Check ifthe correctvoltage is
displayed
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INSTALLATION
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K< Instruction manual SFM
For your notes:
09.83 l INSTALLATION - 4 -

Instruction manual
SFM
1.5 INSTALLATION
The Installation site ofthe Instrument should be suchthat itisnot
subject to dust or vapours of solvents and acids. The ambient
temperatureshould be between 10°C and 35°C.
It is most important thatthere is sufficientfreespace around the
Instrument for adequateVentilation.
air outlet
mini mum 30cm
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09.83
l INSTALLATION
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Hü Instruction manual
SFM
1.5.1 INSTALLATION OFTHE XENON LAMP
ATTENTION: The xenon lamp isa highpressure lamp. Handle it with
care.
EXPLOSION HAZARD!
The lampprotectioncover must be fittedbefore working on
or around the lamp.
Wear a faceprotectionshield while fitting the lamp
protectioncover.
Procedures:
fS
- disconnect mains cable onthe Instrument
-to remove the rear panel unscrew the four
screws (A). Screw one into (B)and remove
the rearpanelcarefully by pulling onthe
screw (B).
- wearfaceprotection
remove the lamp housing by screwing outthe
screws (C)
- carefullyscrew inthe lamp withprotective
cover, anöde (+)onthe top,into the lamp
holder
Turn the centralscrew (D) with thehex
wrench so that the glass ridge (E)onthe
lamp points tothe right.Perform a coarse
positioning ofthe lamp with help ofthe
table (chap. 1.5.2)
- Wind the cathodeconnection cable (K) around
the lamp and with the knurled nut clamp it
onto the cathode (-).
- Remove carefully the lamp protectioncover
Screwdown the cover tothe lamp housing.
Tighten all4 screws (C) equally.
- Faceprotectionshield canbe removed
Remount the backpanelwith the4 screws
(A).
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Instruction manual
SFM
1.5.2 LAMP ADJUSTMENT
Switch onthe Instrument and waitabout 10min
Open the sample compartment cover and remove the accessory (cell
holder, LC-Cell holder)
Adjust the excitation wavelength to540 nm
Hold a white paper or card onthe lens tube inthe excitation beam
The lamp should be adjusted with
the adjusting screws (G,H,I)so
that the highest light intensity is
seen onthe card.
The possibleadjustments ofthe
lamp holder are described below.
Movement of
the lamp
1 down
1 up
v. back
X~ f orward
» right
-« left
Screws
G, H, I
G, H,I,
H
H
I
I
Direction of rotation
clockwise(all 3 equally)
counterclockwise(all 3 equally)
clockwise
counterclockwise
clockwise
counterclockwise
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Instruction manual
SFM
1.5.3 REMOVAL OF THE XENON LAMP
ATTENTION: The xenon lamp isa highpressure lamp.
Handle it with care!
EXPLOSION HAZARD!
The lampprotectioncovermust be fittedbeforeworking on
or around the lamp.
Wear a faceprotectionshield while fitting the lamp
protectioncover.
Switch the Instrument offat least l hourprior tothe
lamp removal. Neverexchange hot lamps.
Procedure: - Disconnect mains plug onthe Instrument
-Letthe Instrument cooldown!
- Remove back panel (screws A)
- Use faceprotection
- Remove cover onthe lamp housing(screws C)
-Fitthe lampprotectioncoveraround the lamp
- Loosen the knurled nutonthe cathode (-)and remove the
cathodeconnection cable (never tryto unplug)
- Screw the lamp outofthe lamp holder andputit back
into the packingbox.
The Installation ofthe xenon lamp is described in 1.5.1.
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3 Instructionmanual
SFM
1.5.4 FITTING THE SAMPLE COMPARTMENT ACCESSORIES
The SFM25is delivered with a cell changer ora HPLC flow cell
The samplecompartmentaccessory is placed into the sample compartment
and fastened with the fixingscrew F.
The front plate P hasan easyremovablesnap fixing. Through the tube
guide, the necessarycables or tubes can easily be led into the sample
compartment.
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•B Instructionmanual
SFM
1.5.5 ADJUSTMENT OFTHE HPLC FLOW GELL
Flow direction out
Flow direction in
max. operatingpressure : 50bar
The flow cell has beenmounted and adjusted inthe factory. A re-
adjustment isnot required.
If there are difficulties while mounting the modul or when in use,then
please contactyourKONTRON representative.
09.84
INSTALLATION
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Kl Instructionmanual
SFM
1.5.6 CONNECTION OF ACCESSORIES
A variety of peripherialequipment canbe connected totheSFM25 e.g.:
Recorder(Y/t and X/Y), Printer, Printer/Plotter or Computer. The
following plugs are already prepared onthe rear panel.
l. Analogue out-
put for Recor-
der and inte-
grator
2. RS-232C for
Printer, Prin-
ter /Plotter
or Computer
Recorder cable
The recordercable canbe plugged in,onthe 15-pol socket (1)
Followingsignal are available:
REM violet(Remote ON/OFF)
GND yellow
REG grey
GND pink
Ared
GND black
Shield blue
INT
GND
white
brown
-a Shieldgreen
Not usedcableends aretobe insulated.
Pin assignment ofRS 232C-Interface (Socket 2)
Recorder
Integrator
GND
DSR
CTS
RxD
DTR
RTS
TxD"
Also see appendix,chapter 7.7
09.84
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Ifl Instructionmanual SFM
2. INSTRUMENT DESCRIPTION
2.1 Specifications 2
2.2 Construction ofthe Instrument 4
2.3 Keyboard 6
2.4 Display 8
2.5 Parameter plan 9
2.5.1 Wavelength control(group 2)11
2.5.2Datacontrol(group 3)12
2.5.3 Printer/Flotter (group 4)14
2.5.4 Interfacecontrol RS-232C (group 5)16
04.84 2 INSTRUMENT DESCRIPTION - l -

Instruction manual
SFM
The SFM25isa highperformanceSpectralfluorometer to measure in the
wavelength ränge of200- 800 nm. It is controlled by3 microprocessors which
allow simple Operationover the keyboard or completeexternal control. The
flexible conception ofthe Instrument gives numerous expansion possibilities.
Both the excitation- and emission monochromator are equippedwithholographic
stigmatic correctedblazedconcave gratings ina Seya-Nomioka-arrangement. The
spectral bandwidth ofthe monochromators is switchable from 10to15nm. Slits
with 5 and10nm bandwidth are available. The light source isa 150W xenon
high pressure lamp. The lamp house is sealed.
The Standardversionincludes a referencebeamSystem.Thisprovideshigh
signal stabilityevenwithtemporary energy variations ofthe lampcaused by
movement ofthe are, shimmering oftheair surrounding the lamp or other
thermal effects. The referencedetector isa photodiode. The lightemitted by
the sample is measured with a photomultiplier. The accessoriessuch äs 4 cell
changer, thermostattedcellholders,HPLC flow cell are built ona modular
basis andare easelyfittedinto the sample compartment.
2.1 SPECIFICATIONS
Optical System
Wavelength ränge
MonochromatorsExcitation and
Emission
Excitation
Emission
Band width
Wavelength accuracy
Wavelength precision
Lamp powersupply
Light sources
Lamp housing
highenergy throughput double
monochromatorSystemwithreference
beam compensation of sourceOutput
changes
200- 800nm
Concaveholographicgratings,
1200 line/mm;
f= 100mm,F 3.5;
linearedispersion = 8 nm/mm
Blazed at250nm
Blazed at450nm
10nm,15nm,20nm selectable
(5nm options)
1.0nm
0.1nm
switchingregulator,powerOutput
regulated
Xenon-Highpressure lamp 150W
sealed
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ä Instruction manual
SFM
2.1 SPECIFICATIONS
Sample detector
Referencedetector
Fluorescencedisplay
Display
Scanspeed
Scanränge
High voltage
Calibration
Blankcompensation
Response time
Sensitivity
Photomultiplier R 212
Range 200-650 nm
(Option:Photomultiplier R446
Range 200-800 nm)
Photodiode
0...+ 200.0
alphanumeric; 16-digit
20-500 nm/min in steps of5 nm/min
1-600 nmin steps ofl nm
100-999 volts automatically selected
by the microprocessor
automatic
automatic
0.5, l,2,4,and8 sec.
for 98%of füll scale
Raman band of water
Ex 350nm;Em397nm; slit 10nm;
Responsetime 8 sec;
S/N better than 70:1
AnalogOutputs:
Fluorescence
Integrator
Wavelength
Remote
DigitalInterface
Power requirements
Dimensions
Weight
Printer/Plotter
100mVfor200% rel. fluorescence
IV for200% rel. fluorescence
100mV/ 1000 nm
TTL - Signal (5V) for Start/Stop
RS-232C bidirectional
Baudrate 110-2400
Format 5-8 bits
Parity odd, even, off
Stopbits l or2
100, 120,220, 240VAC
50 / 60 Hz; 400VA
275mm high; 532mm wide;
517nun deep
37kg
Option
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SS Instruction manual
SFM
2.2 CONSTRUCTION OFTHE INSTRUMENT
Parameterplan
Display field
Keyboard
Sample compartment
PowerON/OFF
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i Instruction manual
SFM
2.2 CONSTRUCTION OFTHE INSTRUMENT
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l) AnalogOutput
2} RS-232C - Interface
3) Mains inlet withselector and fuses
4) Earth terminal
5) Fuses for lamp powersupply(xenon unit]
6)Air outlet
7) Cover plate memory board
8) Coverplate for spareplaces
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2 INSTRUMENTDESCRIPTION
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'•[i Instruction manual
SFM
2.3 KEYBOARD
The keys are divided into 2 groups:
a) Keys for numerical entries
clear
return
Clears the last number entered
Resets errors or error messages(seechapter 4)
AUTO ZERO
Stores the enterednumber and returns tothe measuring mode
Stores the enterednumber and advances tothe next line
to enternumericalvalues and ON/OFFstates
b) Function keys
A T
A-Search:
These keys are used to move the excitation or emission
monochromator manually. The monochromator canbe moved
in singlesteps by short,repeatedpressing onthe
key.
For continuous movement keep thekey pressed.
The monochromatorspeedduring the first 4 sec. is145
nm/min and then 930 nm/min. The monochromator moves
back tothe original value after pushing the "return"
key.
Automatic calibration
Afterpressing thekeythe Instrument sets all para-
meter values so, that the measured fluorescence
intensitycorrespondswith the referencevalueunder
mmm CALIBRATION. The highvoltage onthe photomulti-
plier is regulated andthe valuestoredunder •(*••'•
HIGH VOLTAGE, MM BLANK and •«• FACTOR aresetto
0and1.0 respectively.
10.85
2 INSTRUMENT DESCRIPTION
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