Thermo Scientific Shandon ClearVue A79210100 Manual

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 1
DRAFT
Shandon ClearVue™
Operator Guide - English
A79210100 - Issue 2
S
HANDON
C
LEAR
V
UE

2 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
DRAFT
© 2007 Thermo Fisher Scientific. All rights reserved.
Thermo Shandon Limited is an ISO 9001 and TickIT Accredited Company
Thermo Fisher Scientific is the trading name of Thermo Shandon Limited
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries
Thermo Fisher Scientific makes every endeavour to ensure that the information
contained in its support documentation is correct and clearly stated but does not accept
responsibility for any errors or omissions. The development of Thermo Fisher products
and services is continuous. Make sure that any published information that you use for
reference is up to date and relates to the status of the product. If necessary, check with
Thermo Fisher or your local Thermo Fisher representative.
This manual may not, in whole or in part, be copied, photocopied, reproduced,
translated, or converted to any electronic or machine readable form without prior written
consent of Thermo Fisher.
All information contained in this manual is proprietary and confidential, and the exclusive
property of Thermo Fisher Scientific. This manual is protected by copyright and any
reproduction is prohibited. This manual is for use only by the individuals to whom it has
been made available by Thermo Fisher Scientific.
Contact addresses
Anatomical Pathology
93-96 Chadwick Road
Astmoor, Runcorn
Cheshire, WA7 1PR, UK
Tel: [phone]
Fax: [phone]
www.thermo.com/pathology
Anatomical Pathology
4481 Campus Drive
Kalamazoo,
MI 49008, USA
Tel: [phone]
Fax: [phone]
www.thermo.com/pathology
The Shandon ClearVue meets the following CE Mark requirements:
In Vitro Diagnostic Directive 98/79/EC
Low Voltage Directive 2006/95/EC, as amended by 93/68/EEC.
®
C US
e Shandon ClearVue™ is referred to throughout this document as the Shandon ClearVue.

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 3
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The following symbols and conventions are used throughout this
manual and on the instrument.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT,
TO WARN THAT INSTRUCTIONS MUST BE FOLLOWED FOR SAFE
AND CORRECT OPERATION. IF THIS SYMBOL APPEARS ON THE
INSTRUMENT, ALWAYS REFER TO THIS OPERATOR GUIDE.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT,
TO WARN THAT THERE MAY BE A BIOHAZARD ASSOCIATED
WITH THE INSTRUMENT. ALWAYS ACT WITH COMMON SENSE
AND BE AWARE OF THE SAMPLES USED. TAKE SUITABLE
PRECAUTIONS.
THIS SYMBOL IS USED ON THE EQUIPMENT, OR IN A DOCUMENT,
TO WARN THAT HARMFUL CHEMICALS ARE USED WITH THE
INSTRUMENT. REFER TO THE MATERIAL SAFETY DATA SHEETS
FOR THE CHEMICALS USED. ALWAYS ACT WITH COMMON
SENSE AND BE AWARE OF LOCAL LABORATORY PROCEDURES.
TAKE SUITABLE PRECAUTIONS.
A warning is given in the document if there is a danger of personal
injury or damage to samples or equipment.
Note
Notes give more information about a job or instruction but do not form
part of the instructions.
SymbolsSymbols

4 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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Contents
How to Use This Guide
1 - Introducing the Shandon ClearVue
1-1 - Introduction
1-2 - Coverslip Compatibility
1-3 - Identification of Parts
1-4 - System Interfacing
1-5 - System Specification
1-6 - Coverslipping Method
2 - Basic Operation
2-1 - Daily Tasks
2-2 - Weekly Tasks
2-3 - Changing the Carbon Filter
2-4 - Filling the Xylene Tray
2-5 - Filling the Mountant Bottle
2-6 - Changing the Purge Tray and Debris Tray
2-7 - Starting Up the Shandon ClearVue
2-8 - Filling the Dispense Head Cleaning Station
2-9 - De-Gassing the Mountant Bottle
2-10 - Flushing the System
2-11 - Purging the System
2-12 - Changing the Coverslip Hopper
2-13 - Loading Baskets
2-14 - Unloading Baskets
2-15 - Aborting a Basket
2-16 - Shutdown Procedure

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 5
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3 - Settings
3-1 - Selecting Coverslip Size Options
3-2 - Adjusting Coverslip Position
3-3 - Altering Coverslip Transfer Head Position
3-4 - Screen Options
3-5 - Time and Date Settings
3-6 - Disc Options
3-7 - Altering the Mountant Dispense Volume
3-8 - Changing Languages
4 - Troubleshooting
4-1 - Error Screens
4-2 - Identifying Problems
4-3 - Troubleshooting Tables
5 - Cleaning and Maintenance
5-1 - Cleaning Schedules
5-2 - User Cleaning and Maintenance
5-3 - Taking Out of Operation and Storage
Appendix A - Spares and Accessories
Appendix B - Reagent List
Appendix C - Transportation Instructions
C1 - Things to Do Before Packing
C2 - Packing Instructions
Appendix D - Screen Maps
Appendix E - Declaration of Conformity
Index

6 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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How to Use This Guide
The Shandon ClearVue is a high throughput, automated
coverslipper intended for use in laboratories by operators familiar
with coverslipping techniques and laboratory equipment.
Before operating this instrument the user should have read and
understood the Safety section of the Safety and Warranty
Booklet (A79910001).
Chapter 1 - Introducing the Shandon ClearVue - This chapter
gives a tour of the instrument and its features. It describes the
different parts of the instrument and gives general information
with regards to interfacing with the system.
Chapter 2 - Basic Operation - This chapter is intended to
supplement the information given in the Quick Start Guide
(A79210120).
Chapter 3 - Advanced Operation - This chapter covers the
more advanced features available on the Shandon ClearVue.
The information contained in this chapter is intended to allow
experienced operators to increase the versatility of the Shandon
ClearVue.
Chapter 4 - Troubleshooting - This chapter is intended to help
operators identify and cure common problems.
Chapter 5 - Cleaning and Maintenance - This chapter lists
the day-to-day cleaning routines required for the safe and
reliable operation of the Shandon ClearVue. It also contains the
methods required to perform many of the remedies listed in the
Troubleshooting section of this document.
IntroductionIntroduction
Chapter
Summary
Chapter
Summary

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 7
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1 - Introducing the Shandon ClearVue
The Shandon ClearVue is a high-throughput, precision engineered,
slide coverslipper, capable of coverslipping 400 slides every hour
in a precise and efficient manner.
The Shandon ClearVue has been designed for general laboratory
use, and can handle baskets from the following stainers:
Shandon Varistain®Gemini (Inc. ES Variant)
Shandon Varistain®24-4
Leica Autostainer
Sakura Tissue-Tek®DRS 2000™ Series
The Shandon ClearVue is capable of having multiple baskets
loaded at once in any order from any of the above stainers.
The Shandon ClearVue is compatible with the following sizes of
coverslips:
No. 1.0 x 24 x 40 mm
No. 1.0 x 24 x 50 mm
No. 1.5 x 24 x 40 mm
No. 1.5 x 24 x 50 mm
No. 1.5 x 24 x 55 mm
The following slide dimensional tolerances are permissible:
Length 74.5 -76.0 mm
Width 24.5 - 26.0 mm
Thickness 0.8 - 1.2 mm
•
•
•
•
•
•
•
•
•
1-1 - Introduction1-1 - Introduction
1-2 - Compatibility1-2 - Compatibility

8 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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1-3 - Identification
of Parts
1-3 - Identification
of Parts
1
23
4
5
17
12
16 15 14
13
8
11
6
7
10
1 - Load Door
2 - Unload Door
3 - Prep Door
4 - Touch Screen
5 - Mountant Bottle
6 - Load Rail
7 - Xylene Tray
8 - Unload Rail
9 - Downdraft Ventilation
10 - Prep Area
11 - Debris Tray Access Door
12 - Filter Cover
13 - Mains Power Switch and Connector
14 - 3½” Floppy Disk Drive
15 - Rating Label
16 - Vents for Electronics Enclosure
17 - Battery Isolation Switch
9
1
23
4
5
17
12
16 15 14
13
8
11
6
7
10
1 - Load Door
2 - Unload Door
3 - Prep Door
4 - Touch Screen
5 - Mountant Bottle
6 - Load Rail
7 - Xylene Tray
8 - Unload Rail
9 - Downdraft Ventilation
10 - Prep Area
11 - Debris Tray Access Door
12 - Filter Cover
13 - Mains Power Switch and Connector
14 - 3½” Floppy Disk Drive
15 - Rating Label
16 - Vents for Electronics Enclosure
17 - Battery Isolation Switch
9

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 9
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Load Door
Unload Door
Access Door
Touch Screen
Mountant Bottle
Load Rail
Xylene Tray
Unload Rail
Downdraft Ventilation
Coverslip Preparation Area
Debris Tray Door
Filter Cover
Main Power Switch, Fuses and Connector
3½” Floppy Disk Drive
Rating Label
Vents for Electronics Enclosure
Battery Isolation Switch
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
1
23
4
5
17
12
16 15 14
13
8
11
6
7
10
1 - Load Door
2 - Unload Door
3 - Prep Door
4 - Touch Screen
5 - Mountant Bottle
6 - Load Rail
7 - Xylene Tray
8 - Unload Rail
9 - Downdraft Ventilation
10 - Prep Area
11 - Debris Tray Access Door
12 - Filter Cover
13 - Mains Power Switch and Connector
14 - 3½” Floppy Disk Drive
15 - Rating Label
16 - Vents for Electronics Enclosure
17 - Battery Isolation Switch
9
1
23
4
5
17
12
16 15 14
13
8
11
6
7
10
1 - Load Door
2 - Unload Door
3 - Prep Door
4 - Touch Screen
5 - Mountant Bottle
6 - Load Rail
7 - Xylene Tray
8 - Unload Rail
9 - Downdraft Ventilation
10 - Prep Area
11 - Debris Tray Access Door
12 - Filter Cover
13 - Mains Power Switch and Connector
14 - 3½” Floppy Disk Drive
15 - Rating Label
16 - Vents for Electronics Enclosure
17 - Battery Isolation Switch
9

10 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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Orientation
Indicator for
loading onto
ClearVue
Orientation
Indicator for
slide loading
Slider
Location
Hook
Slide
Shelves
Orientation
Indicator for
loading onto
ClearVue
Orientation
Indicator for
slide loading
Slider
Location
Hook
Slide
Shelves

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 11
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The Touch Screen panel is the primary user interface mode on
the Shandon ClearVue.
It is used to input data, operate the manual functions and inform
the user of instrument data.
In addition, the Shandon ClearVue will issue audible alerts when
appropriate.
General operation of the Shandon ClearVue is started by opening
and closing the Load Door.
Stopping and restarting the Shandon ClearVue is controlled by the
software to ensure safety, whilst at the same time making sure
that the samples are not compromised.
Opening any of the doors will not necessarily cause the Shandon
ClearVue to stop running; therefore care should be taken when
adding or removing baskets.
1-4 - System
Interfacing
1-4 - System
Interfacing

12 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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Dimensions
Height 500 mm 19.7”
Width 645 mm 25.4”
Depth 575 mm 22.6”
Weight 48 kg 106 lbs
Electrical Specification
Voltage 100 - 240 Vac
Frequency 50 / 60 Hz
Power (max) 300 VA
Earth Leakage < 500 mA at 110-120 Vac
Fuses T 6.3 A, 250 V
Internal Batteries Sealed Lead Acid type.
Not user replaceable.
Environmental Requirements
Warning - For Indoor Use Only
Temperature
(Operating Limits) +5°C to +40°C (+41°F to +104°F)
Temperature
(Recommended
Operation)
+15°C to +30°C (+59°F to +86°F)
Note: Performance may deteriorate when
operated outside of this temperature range.
Temperature
(Storage)
-25°C to +55°C (-13°F to 131°F)
+70°C (158°F) for short exposure
Relative Humidity Max. 80% RH up to 31°C
Decreasing linearly to 50% RH at 40°C
Altitude Up to 2000 m (6,500 ft)
Pollution Degree 2
Over Voltage
Category II
1-5 - System
Specification
1-5 - System
Specification

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 13
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The Shandon ClearVue has been specifically designed to ensure
consistent, high quality, coverslipping.
The following diagram shows how the coverslip is laid:
This method of coverslipping has been proven, through rigorous
testing, to minimize bubbles and ensure complete adhesion to the
slide.
1-6 - Coverslipping
Method
1-6 - Coverslipping
Method

14 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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2 - Basic Operation
The following chapter describes the basic operational tasks
required to run the Shandon ClearVue on a daily basis.
2-1 - Daily Tasks
2-2 - Weekly Tasks
2-3 - Changing the Carbon Filter
2-4 - Filling the Xylene Tray
2-5 - Filling the Mountant Bottle
2-6 - Changing the Purge Tray and Debris Tray
2-7 - Starting up the Shandon ClearVue
2-8 - Filling the Dispense Head Cleaning Station
2-9 - De-Gassing the Mountant Bottle
2-10 - Flushing the System
2-11 - Purging the System
2-12 - Changing the Coverslip Hopper
2-13 - Loading Baskets
2-14 - Unloading Baskets
2-15 - Shutdown Procedure

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 15
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The following tasks should be carried out at least once a day:
Top-up the Dispense Head Cleaning Station -
see Section 2-8.
Wipe the Dispense Head with a Xylene damp
cloth.
Wipe the top of the Dispense Head Cleaning
Station with a Xylene damp cloth.
Check the number of Coverslips in the Hopper
and replace if necessary - see Section 2-12.
Check the level of Mountant and top-up if
necessary - see Section 2-5.
Ensure that the Suction Cup is clean and free
from debris - Replace if necessary - see Section
5-2-4.
2-1 - Daily Tasks2-1 - Daily Tasks

16 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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The following tasks should be carried out at least once a week:
Empty, clean and refill the Dispense Head
Cleaning Station - see Section 5-2-3.
Remove any discarded coverslips from the Slip
Dispense Area.
Remove the Coverslip Transfer Head - see
Section 5-2-4 - to check that the Pads and Suction
Cup are clean and free of Mountant.
Replace the Suction Cup if necessary - see
Section 5-2-4 - and wipe the Pads with a xylene
damp cloth to clean
Top up the Xylene Tray - see Section 2-4
Empty the Debris Tray - see Section 2-6.
Check the Purge Tray and replace if necessary
- see Section 2-6.
2-2 - Weekly Tasks2-2 - Weekly Tasks

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 17
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The Carbon Filter should be changed every 3 Months to ensure
it functions correctly.
To change the Carbon Filter:
Slide the Filter Cover off to gain access to the
Carbon Filter.
Lift the Carbon Filter out of the chamber and
dispose of according to local regulations.
Remove the cellophane wrapping from a new
Carbon Filter and place into the chamber.
1.
2.
3.
2-3 - Changing
the Carbon Filter
2-3 - Changing
the Carbon Filter

18 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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Slide the Filter Cover back into position making
sure it is firmly clipped into place.
The Carbon Filter should be changed every 3 months - write the
installation date on the Carbon Filter using a marker to ensure
proper record keeping.
4.

Shandon ClearVue™ Operator Guide A79210100 - Iss 2 19
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The Xylene Tray is intended to provide a xylene rich atmosphere
to prevent slides on the Load Rail from drying out.
The Xylene Tray should be checked every week and topped up
as required.
To fill the Xylene Tray:
Slide the Xylene Tray out about half-way, taking
care not to spill any remaining xylene.
Carefully pour xylene into the Xylene Tray.
1.
2.
2-4 - Filling the
Xylene Tray
2-4 - Filling the
Xylene Tray
Anti-Surge Baffle
Xylene - Harmful
Anti-Surge Baffle
Xylene - Harmful
Anti-Surge Baffle
Xylene - Harmful
Anti-Surge Baffle
Xylene - Harmful

20 A79210100 - Iss 2 Shandon ClearVue™ Operator Guide
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The depth of the xylene should be no higher
than the top of the Anti-Surge Baffle.
Slowly close the Xylene Tray completely to
ensure that the seal is properly engaged.
3.
4.
Anti-Surge Baffle
Xylene - Harmful
Anti-Surge Baffle
Xylene - Harmful
Anti-Surge Baffle
Xylene - Harmful
Anti-Surge Baffle
Xylene - Harmful