Lab-Line 314 User manual
1/01
OPERATION MANUAL
MANUAL NO. 057-561-00
REV. A
LAB-LINE®
AIR-JACKETED COMPACT
AUTOMATIC & FLO-THRU®
CO2 INCUBATORS
MODEL NO. AUTOMATIC:
314, 314-1, 314-1CE
(Stainless Steel Interiors)
316, 316-1
(Copper Interiors)
FLO-THRU:
315, 315-1
(Stainless Steel Interiors)
317, 317-1
Copper Interiors)
DESIGNERS AND MANUFACTURERS
A SUBSIDIARY of Barnstead|Thermolyne
1999 North 15th Ave., Melrose Park, IL 60160-1491 USA
PHONE: (319) 556-2241 or (800) 522-5463; FAX: (319) 589-0516
2
TABLE OF CONTENTS
3
SECTION TITLE
1 Introduction
2 Description
3 Specifications
4 Features
5 Installation
6 Operation
7 Maintenance
8 Replacement Parts
Warranty
4
BE ADVISED:
IT IS MOST IMPORTANT THAT THE USER FOLLOW INSTALLATION
INSTRUCTIONS EXACTLY AS WRITTEN—FAILURE TO DO SO IS LIKELY TO
LEAD TO IMPROPER OPERATION, ERRONEOUS CALIBRATIONS AND
POSSIBLE DAMAGE TO THE EQUIPMENT. UNDER NO CIRCUMSTANCES
SHOULD THE USER ATTEMPT OPERATION WITHOUT THIS INFORMATION.
THE FOLLOWING EQUIPMENT IS TO BE SUPPLIED BY THE USER:
•Dry CO2gas (research grade or better).
•A dual-stage regulator for the CO2tank.
•¼" ID Flexible tubing (appropriate length from tank to incubators), and
connected per local codes.
•Fyrite or similar chemical-based CO2analyzer.
BE ADVISED: INSTALLATION AND PRE-OPERATION PROCEDURE WILL
TAKE AT LEAST 24 HOURS TO COMPLETE. DO NOT ATTEMPT TO RUSH
THE PROCESS WITH SHORT CUTS TO THE PROCEDURES DESCRIBED IN
THIS MANUAL.
5
CERTIFICATION OF DECONTAMINATION:
We cannot accept for service or credit a product that has been exposed
to or contaminated with chemically or biologically toxic or infectious substances
or subjected to radioactivity without first being certified as free from said
contamination.
Please have your Medical and/or Safety Officer sign this form certifying
that proper decontamination procedures have been followed to render the
product safe and free from hazards.
Any product forwarded to us, not accompanied by this form and a proper
Return Goods Authorization Number, will be returned to the sender. To obtain a
Return Goods Authorization Number, contact the Customer Relations
Department at (800) 522-5463.
We hereby certify that the LAB-LINE INSTRUMENTS, INC. product:
Model No. and Serial No. ,
which is being forwarded has been properly decontaminated and is free from all toxic
hazards, infectious agents, radioactivity and/or other hazards.
Company/Institution Name:
Street Address:
City: State Zip
Name (please print): Title
Signature:
Phone:
DECONTAMINATION PROCEDURE (Be Specific):
Nature of Hazard That Required Decontamination:
6
7
SECTION1
INTRODUCTION
THANK YOU
for selecting Lab-Line Instruments for your equipment needs. For maximum value and
ease of start-up,
PLEASE PROCEED AS FOLLOWS:
•Inspect the carton and contents for shipping damage. Notify the carrier immediately
if damage is found.
•Use the Accessory Checklist when unpacking to verify that the complete unit has
been received. Do not discard packing materials until all is accounted for.
•Read this Operation Manual thoroughly before deciding upon an appropriate location
for the unit: you will want to consider the availability of power and other unit
requirements, as well as user convenience.
•Insist that every operator of this unit becomes familiar with the Operation Section of
this manual.
•Be sure to fill out the Warranty Registration Card and mail it in to Lab-Line
Instruments within seven (7) days after receiving the unit.
IF
after reading this manual you should have any difficulties with the installation or
operation instructions, please call:
Lab-Line Customer Relations Department
(319) 556-2241 or (800) 522-5463
ALL RIGHT RESERVED
The information contained in this manual is the exclusive property of Lab-Line Instruments, Inc., and has been pro-
vided solely to enable the users of the equipment described herein to operate and maintain such equipment. Any
other use of this information, or the reproduction or transmission of all or any portion of this manual without prior
written consent of the manufacturer is expressly prohibited. © 2001, Lab-Line Instruments, Inc.
SECTION 2
8
DESCRIPTION
314/316 SERIES AUTOMATIC CO2INCUBATORS:
BASIC COMPONENTS, AN INITIAL OVERVIEW:
1 13
14
2
3
4
5
6
7
8
15 9 10 11 12
1. Control panel
2. CO2sensor
3. CO2inlet port
4. Temperature sensor
5. Chamber gasket
6. Chamber shelves
7. Sample port inlet
8. Leveling feet
9. Inner glass door, handle shaft
10. Inner glass door
11. Inner glass door, handle
12. Outer door gasket
13. CO2sample port
14. Fan Cover
15. Humidity Trough
DESCRIPTION:(Con’t)
9
314/316 SERIES AUTOMATIC CO2INCUBATORS: (Con’t)
BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T)
CHAMBER CEILING, INTERIOR
CIRCULATING FAN
REAR PANEL VIEW, EXTERIOR:
1 4
2 5
3 6
1. GAS INLET PORT
2. CORDSET
3. CIRCUIT BREAKERS
4. RS232 DATA PINS CONNECTOR
5. POWER SWITCH
6. OPTIONAL ALARM RELAY OUTPUT
DESCRIPTION:(Con’t)
314/316 SERIES AUTOMATIC CO2INCUBATORS:
10
CO2levels are automatically set at 5%. These units control CO2
concentrations, as well as temperature, to create a growth environment ideal for
tissue cultures, plants, cells, viruses and related organisms.
Incoming 100%, CO2, from an external source provided by the user,
passes into the gas inlet port located on the rear panel of the unit. Heated air is
moved uniformly throughout the chamber by a circulating fan located in the top of
the chamber. A CO2access port on the control panel permits sampling of the
chamber atmosphere.
These incubators provide precise and reliable control of carbon dioxide
through the use of a TC, thermal conductivity, sensor coupled with an easy-to-
use command control console. All required operating commands are
immediately accessible in a functional format on the front of the unit and permit
rapid start-up, close monitoring and efficient control of incubation protocols.
Temperature is measured by a platinum RTD, resistance temperature
detector, producing excellent control within the range, ±0.1ºC. This is combined
with the exclusive SmartGas® algorithm method for controlling CO2within the
range ±0.1%. The result is a chamber that produces excellent environmental
conditions for the most sensitive of incubations.
Audible and visual alarms provide a signal to the user of any deviation in
the chamber temperature of ±1.0ºC, or carbon dioxide of ±1.0% CO2. In
addition, other signals alert the user to power failures and door openings.
An independent, adjustable hydraulic high limit thermostat will limit the
temperature in the event of a problem with the main temperature controller.
To conserve gas, the unit’s CO2solenoid valve, as well as the circulating
fan motor, shuts off automatically any time the door is opened.
A double-door system has a tempered glass inner door that provides
unobstructed viewing of the entire chamber.
314 series chambers are constructed of seamless stainless steel for
cleaning ease and maintenance; 2 stainless steel shelves are standard—a total
capacity is 5 shelves. 316 series incubators feature a fungus and bacteria
resistant copper chamber.
Both models have a stainless steel outer cabinet with a powder coated
finish for easy cleaning.
DOUBLE-DOOR CONSTRUCTION WITH INNER DOOR DEFOGGER: 314/316
MODELS:
A tempered glass inner door allows observation of a chamber without
disturbing the chamber environment. Inside the steel outer door are heaters
that can be turned on to defog the glass door when necessary. Each door
seals to a one-piece gasket for a leak-tight environment.
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DESCRIPTION:(Con’t)
315/317 SERIES FLO-THRU CO2INCUBATORS:
BASIC COMPONENTS, AN INITIAL OVERVIEW:
1
2 3 4 5 6 7 8 9 10
1. AIR & CO2
FLOWMETERS
2. UNIT FEET
3. CO2 ACCESS PORT
4. CHAMBER SHELVES
5. TEMPERATURE SENSOR
6. CHAMBER GASKET
7. INNER GLASS DOOR, HANDLE SHAFT
8. INNER GLASS DOOR
9. INNER GLASS DOOR, HANDLE
10. OUTER STEEL DOOR/GASKET
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DESCRIPTION:(Con’t)
315/317 SERIES FLO-THRU CO2INCUBATORS: (Con’t)
BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T)
CHAMBER CEILING, INTERIOR
CIRCULATING FAN
REAR PANEL VIEW, EXTERIOR:
CO2
1 2
1. CO2 INLET PORT
2. CORDSET
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DESCRIPTION:(Con’t)
315/317 SERIES FLO-THRU CO2INCUBATORS:
CO2 levels are manually adjusted utilizing flowmeters. Flo-Thru control of
CO2concentrations and temperature create a growth environment ideal for tissue
cultures, plants, cells, viruses and related organisms.
An incoming gas mixture passes through the plenum and is gently
circulated throughout the chamber. Separate flowmeters adjust air and CO2
rates and an access port on the control panel permits sampling of the chamber
atmosphere.
2 hydraulic thermostats: one is an adjustable setpoint control unit, the
other is a non-adjustable, over-temperature-limiting unit. It is factory pre-set to
regulate temperature in order to protect the chamber.
Heaters on the outside of the chamber inner walls are positioned for
uniform heat distribution, while high-density fiberglass insulation minimizes heat
loss.
A double-door system has a tempered glass inner door that provides
unobstructed viewing of the entire chamber.
Model 315 and 315-1 interiors are constructed of seamless stainless steel
for cleaning ease and maintenance. 2 stainless steel shelves are standard—a
total capacity is 5 shelves.
Models 317 and 317-1 have a seamless copper interior that acts to reduce
the growth of fungus and bacteria thereby minimizing contamination of
cultures. 2 copper shelves are standard with room for 3 additional shelves.
All models have a steel outer cabinet with a powder coated finish for easy
cleaning.
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SECTION 3
SPECIFICATIONS
314/316 SERIES AUTOMATIC CO2 INCUBATORS:
ELECTRICAL REQUIREMENTS:
314, 316: 120 V, 60 Hz, 200 Watts, 1.7 Amps,
314-1, 316-1: 230/240 V, 50 Hz, 200 Watts, 0.8 Amps,
TEMPERATURE:
RANGE: Ambient +5ºC to 60ºC
CONTROL: ±0.1ºC at 37ºC.
UNIFORMITY IN CHAMBER: Within the chamber, ±0.25ºC
ALGORITHM: TEMPERATURE: PID
CO2: SmartGas®
CARBON DIOXIDE TENSION: Range: 0% to 20%
Control: ±0.1%
VOLUME: 1.2 cubic feet (34 liters)
EXTERIOR: 22½" W x 18" D x 19" H
(57 x 46 x 48 cm)
INTERIOR: 14" W x 12" D x 12" H
(36 x 31 x 31 cm)
SHELVES: 2 standard; space for 3 additional shelves.
DIMENSIONS: 13-3/8” (tapering back to) 12-1/8" W x 11½" D
(34~30.7 x 29.2 cm)
SHIPPING WEIGHT: 70 lbs. (32 kg)
SPECIFICATIONS:(Con’t)
15
315/317 SERIES FLO-THRU CO2INCUBATORS:
ELECTRICAL REQUIREMENTS:
315, 317: 120 V, 60 Hz, 200 Watts, 1.7 Amps,
315-1, 317-1: 230/240 V, 50 Hz, 200 Watts, 0.8 Amps,
TEMPERATURE:
RANGE: Slightly above ambient to 60ºC.
CONTROL: ±0.5ºC.
UNIFORMITY: ±1.0ºC
CARBON DIOXIDE RANGE: From 0 to 20% of the mixture.
VOLUME: 1.2 cubic feet (34 liters)
DIMENSIONS:
EXTERIOR: 23½" W x 17" D x 15" H
(60 x 43 x 38 cm)
INTERIOR: 14" W x 12" D x 12" H
(36 x 31 x 31 cm)
SHELVES: 2 standard; space for 3 additional shelves.
DIMENSIONS: 11"W x 13½"D x 7"H (28 x 34 x 18 cm)
SHIPPING WEIGHT: 70 lbs. (32 kg)
UNIT’S ENVIRONMENTAL OPERATING CONDITIONS:
POLLUTION DEGREE: 2
INSTALLATION CATEGORY: II
ALTITUDE: 2000 Meters MSL (Mean Sea Level)
HUMIDITY: 80% maximum, non-condensing
ELECTRICAL SUPPLY: 120VAC or 240VAC
VOLTAGE TOLERANCE: ±10% of normal rated line
TEMPERATURE: 15ºC to 40ºC
PRODUCT USAGE: This product is intended for use indoors only
SECTION 4
16
FEATURES
314/316 series control panels have a series of command keys that enable
users to enter set point values for temperature, carbon dioxide, door heat and to
calibrate a unit as required. LED readout provides users with current status
information regarding the foregoing parameters.
CONTROL PANEL, 314/316 SERIES AUTOMATIC CO2 INCUBATORS:
SET CAL CO2 TEMP DOOR SCAN
ºC %CO2 HEAT HOLD HI LIMIT SAMPLE
1 2 3 4 5 6 7 8 9 10 11 12
1. LED READOUT: The LED displays current CO2tension and temperature
in the Incubator and, in the set mode, displays the set point values
for these two parameters as well as door heat setting. It also enables the
user to enter values necessary to calibrate temperature and CO2.
Indicating lights and legends serve to identify which parameter is being
displayed.
2. SET COMMAND KEY: The SET key is the beginning point for and
initiates subsequent actions of the other keys on the control panel.
3. CAL COMMAND KEY: The CAL key permits calibration of temperature
and CO2when in this command mode. The Up and Down arrow keys are
used to change the readout to correspond with the readings obtained from
known standards of temperature or CO2measurement. Both temperature
and CO2calibration use the zero-point shift method.
FEATURES:(Con’t)
CONTROL PANEL: (Con’t)
17
SET CAL CO2 TEMP DOOR SCAN
ºC %CO2 HEAT HOLD HI LIMIT SAMPLE
1 2 3 4 5 6 7 8 9 10 11 12
4. CO2COMMAND KEY: Pressing the SET key and then the CO2key
allows the user with the Up and Down keys to enter the desired carbon
dioxide level in the Incubator.
•High and low limit alarm set points are factory set at ±1% above and
below the user-established set point. In the event CO2exceeds the
indicated limits, an audible alarm will sound and indicating light above
the key will flash.
•Pressing any key will silence the alarm for a period of one hour. The
user should immediately investigate the reason for the alarm and
take steps to correct the problem. Note that the indicating light
continues to flash. If a problem is not solved the alarm resumes.
•By pressing the CAL (#3) and CO2(#4) keys sequentially users can
calibrate CO2. The Up and Down arrow keys will change the readout
to correspond with the readings obtained from known standards of
CO2measurement.
18
FEATURES:(Con’t)
CONTROL PANEL: (Con’t)
SET CAL CO2 TEMP DOOR SCAN
ºC %CO2 HEAT HOLD HI LIMIT SAMPLE
1 2 3 4 5 6 7 8 9 10 11 12
5. TEMP COMMAND KEY: Pressing the SET key and then the TEMP
command key allows the user with the Up and Down arrow keys to
establish the desired set point temperature in the Incubator.
•High and low limit alarm set points are factory-set at ±1ºC. If the
temperature deviates from these limits, an audible alarm (continuous
beep) will sound.
•While pressing any key will silence the alarm for a one-hour period,
the user should immediately investigate the reason for the alarm and
take steps to correct the problem. Note that indicating light continues
to flash. If problem is not corrected, the alarm will resume after one
hour has passed.
•By pressing the CAL (#3) and TEMP (#5) keys sequentially users can
calibrate temperature. The Up and Down arrow keys will change the
readout to correspond with the readings obtained from known
standards of temperature measurement.
NOTE: DECREASES IN TEMPERATURE AND CO2 DUE TO NORMAL DOOR OPENINGS ARE
TOLERATED WITHOUT INITIATING THE ALARMS.
19
FEATURES:(Con’t)
CONTROL PANEL: (Con’t)
SET CAL CO2 TEMP DOOR SCAN
ºC %CO2 HEAT HOLD HI LIMIT SAMPLE
1 2 3 4 5 6 7 8 9 10 11 12
6. DOOR HEAT COMMAND KEY:
•When the SET key is pressed and then the DOOR-HEAT key, enter
the level of heat with the Up and Down keys as a percentage value
being applied to the inner glass door.
•A flashing DOOR-HEAT indicator light above the key indicates that
heat is being applied to the inner glass door.
•A good starting point for the door heat control is 35%; if fogging still
occurs, increase in 10% increments until fogging disappears.
7. SCAN/HOLD COMMAND KEY: Permits holding the value of a single
parameter for continued viewing or scans alternately between the values
of the two parameters on a 2-second interval.
8. DOWN ARROW KEY: This key decreases numerical value of the
parameter which has been accessed.
9. UP ARROW KEY: This key increases the numerical value of the
accessed parameter.
NOTE: A 5 SECOND DELAY AFTER ANY FINAL ENTRY RESULTS IN AUTOMATIC ENTRY
OF THAT CHANGE.
20
FEATURES:(Con’t)
CONTROL PANEL: (Con’t)
SET CAL CO2 TEMP DOOR SCAN
ºC %CO2 HEAT HOLD HI LIMIT SAMPLE
1 2 3 4 5 6 7 8 9 10 11 12
10. HIGH LIMIT THERMOSTAT: This is a back-up high limit temperature
thermostat which functions should the microprocessor fail. The high limit
thermostat will then control the temperature at slightly above the set point.
11. HIGH LIMIT STATUS LAMP: Indicates when the high limit is active.
12. SAMPLE PORT: This access port is available for sampling the
atmosphere in the chamber when executing calibration procedure such as
might be done using a Fyrite or similar chemical-based CO2analysis
procedure.
FEATURES:(Con’t)
This manual suits for next models
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