LabCo 550.001.305 User manual

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Bottle Top Dispenser
User Manual
Product Code
Description
550.001.305
1 to 5mL
550.001.310
2 to 10mL
550.001.325
2.5 to 25mL
550.001.350
10 to 50ml
Please read the User Manual carefully before use, and follow all operating
and safety instructions!
Technical specifications and outline are subject to change without prior notice.
Version 1.1

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Contents
Introduction
3
Warranty
3
Delivery
3
1. Safety Instructions
3
2. Components
4
3. Operating Exclusions
5
4. Operating Limitations
5
5. Preparing instrument for use
6
6. Mounting instrument on bottle
8
7. Priming the instrument
8
8. Cleaning
11
9. Autoclaving
12
10. Checking the Volume
13
11. Trouble Shooting
14
12. Technical Data
15
13. Return for Repair
15

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Introduction
Welcome to the Bottle top dispenser User Manual. Users should read this manual carefully,
follow the instructions and procedures, and beware of all the cautions when using this
instrument.
Warranty
This instrument is warranted for a period of 12 months from the date of invoice. All warranties
are null and void if the product has been misused, modified or repaired by unauthorised
personnel, if the defects are caused by negligence (instruction manual, maintenance) or by
normal wear and tear. Use only original manufacturer’s accessory/spare parts.
Delivery
This unit is supplied with 1 x main unit, user manual, discharge tube, mounting tool, filling tube
and adapters of PP of the following sizes:
Nominal vol. tube
Adapter, PP
Filling
0.5 –5, 1 –10, 2.5 –25, 5 - 50
GL 25, GL 28, GL 32,GL 38, S40
220 mm
1. Safety Instructions
This instrument may be used with approved hazardous materials (refer to chemical
compatibility chart), operations, and equipment. This manual does not purport to address all
of the safety problems associated with its use. It is the responsibility of whoever uses this
instrument to consult and establish appropriate safety and health practices and determine the
applicability of regulatory limitations prior to use.
a. Follow the general instructions for hazard prevention and safety regulations, e.g., wear
protective clothing, eye protection and gloves.
b. Observe the reagent manufacturer’s information.
c. Every user must be acquainted with this Operating Manual before operation.
d. Use the instrument only for dispensing liquids, with strict regard to the defined Operating
Exclusions and Limitations. If in doubt, contact the manufacturer or supplier.
e. When dispensing, the discharge tube must always point away from the user or any other
person. Avoid splashes. Only dispense into suitable vessels.
f. Never carry the mounted instrument by the cylinder sleeve or the valve block. Breakage
or loosening of the cylinder may lead to personal injury from chemicals.
g. Never press down the piston when the closure cap is pushed on.
h. Never remove the discharge tube while the cylinder is filled.
i. Use smooth gentle movements to operate the piston upwards and downwards.
j. Use only original manufacturer’s accessories and spare parts. Don't carry out technical
modifications.
k. Before using check the instrument for visual damages. In case of trouble (e.g., piston
difficult to move, sticking valves or leakage), immediately stop dispensing. Clean the
instrument according to the cleaning instructions.

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2. Components
1. Piston seat
2. Volume adjustment knob
3. Scale pointer
4. Cylinder sleeve
5. Piston
6. Glass cylinder
7. Seal
8. Discharge valve
9. Discharge tube sleeve
10. Discharge tube
11. Closure cap
12. Filling seal
13. Filling valve
14. Filling tube
15. Air vent cap
16. Valve block
1.
2.
3.
4.
7.
8.
9.
11.
10.
12.
13.
14.
5.
6.
15.
16.
Application and Design
This instrument is designed for dispensing liquids, observing the following limits:
15 to 40 °C of instrument and reagent
vapor pressure up to 500 mbar
density up to 2.2 g/cm3
Materials
When the instrument is correctly used, the dispensed liquid comes into contact with only the
following chemically resistant materials: Borosilicate glass, PFA, FEP, PTFE, Hastelloy; PP
(closure cap).

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3. Operating Exclusions
Never use this instrument for
Liquids attacking FEP, PFA, PTFE or Hastelloy.
Liquids attacking borosilicate glass (e.g., hydrofluoric acid)
Liquids which are decomposed catalytically by Hastelloy (e.g., H2O2)
Explosive liquids.
Suspensions as solid particles may clog or damage the instrument.
4. Operating Limitations
Concentrated hydrochloric acid and concentrated nitric acid, chlorinated and fluorinated
hydrocarbons or liquids which form deposits may make the piston difficult to move or may
cause jamming. When dispensing flammable media, make provisions to avoid static charging,
e. g., do not dispense into plastic vessels; do not wipe instruments with a dry cloth.
Warning!
If there is a sign of a potential malfunction (e.g., piston difficult to move) never use force.
Immediately stop dispensing and follow cleaning instructions or contact the manufacturer.
Note:
Compatibility of the instrument for this special application (e. g., trace material analysis) must
be checked by the user or contact the manufacturer.

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5. Preparing instrument for use
Attention! The adapters supplied with the instrument are made of polypropylene (PP), and can
only be used for media which does not attack PP.
Warning! Wear protective clothing, eye protection and gloves. Follow all Safety Instructions
and observe the Operating Exclusions and Limitations.
Figure 1
Warning! Always wear protective gloves when touching the instrument and the bottle,
especially when using dangerous liquids. Carry the mounted instrument only as shown in
figure 1.

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Connecting the discharge tube
Warning! Do not use any other type of discharge tube. Never use damaged or deformed
discharge tubes.
1.
2.
Figure 2
1. Firmly tighten the discharge valve.
2. Push the discharge tube as far as
possible into the discharge valve.
Connecting the filling tube
1.
2.
Figure 3
1. Cut off the bottom of the filling tube diagonally.
2. Push the filling tube into the valve block as far as possible –with the diagonal end down.

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6. Mounting instrument on bottle
The instrument can be screwed directly onto a GL 45 screw thread. For other bottles, use the
accompanying adapters.
Attention! The adapters supplied with the instrument are made of polypropylene (PP), and can
only be applied to media which do not attack PP.
7. Priming the instrument
Attention! For small bottles use a bottle stand to prevent tipping over.
Warning! Reagent may drop off the discharge tube and closure cap.
Figure 4
Hold the discharge tube and carefully remove the closure cap.
Slide the closure cap backwards on the tube support, away from the discharge tube
opening.

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Figure 5
1. Hold an appropriate collecting vessel under the discharge tube opening.
2. Gently pull the piston up, then press it down rapidly.
3. Repeat this procedure until most of the air bubbles in the glass cylinder and
discharge tube have been removed.
Note: Before using the instrument for the first time, ensure it is rinsed carefully or discard first
few samples dispensed.
Priming/Dispensing
Figure 6

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Warning! Follow all Safety Instructions and observe the Operating Exclusions and Limitations.
The discharge tube must always point away from the user or any other person. Never press
down the piston when the closure cap is pushed on. Reagent may drop off the discharge tube
and closure cap
Setting the volume
1.
2.
3.
Figure 7
Figure 8
Hold an appropriate collecting vessel under the discharge tube opening.
Pull the piston up slowly and evenly to the upper stop to draw reagent into the glass cylinder.
Push the piston down gently and evenly to dispense the reagent into the vessel.
Attention! After use, always leave the piston in the down position.

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8. Cleaning
The instrument must be cleaned in the following situations to assure correct operation:
if the piston becomes difficult to move
after dispensing liquids which form deposits
before changing the reagent
prior to long term storage
should liquid have accumulated in the closure cap
prior to sterilization
before replacing the valves
Warning! Follow all Safety Instructions. The glass cylinder, valves, filling and discharge tubes
contain reagent. Wear protective clothing, eye protection and appropriate hand protection.
Note: Never change the pistons of the instruments!
1) Push on the closure cap.
2) Empty and unscrew the instrument from the bottle and lift the instrument so that the
filling tube is out of the liquid but still in the bottle.
3) Carefully tap the filling tube against the bottle from the inside so that the reagent runs
back into the bottle.
4) Remove the closure cap, and dispense the remaining reagent into the bottle by repeated
piston operation.
5) Mount the instrument onto another bottle filled with an appropriate cleaning solution.
6) Flush the instrument.
7) Empty the bottle, flush it and fill it with water. Repeat step 6 and 7.
8) Empty the instrument.
9) Unscrew the piston seat (1).
10) Carefully pull the piston (5) out of the glass cylinder (6) by pulling on the piston seat only.
11) Carefully remove deposits at the edge of the glass cylinder with a screw driver. Clean
piston and cylinder with a bottle-brush.
12) Carefully reassemble the instrument in the reverse order. Flush instrument with distilled
water.
Figure 9 Figure 10 Figure 11
Note: Insert the piston straight into the cylinder, not at an angle.

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Cleaning/replacing the filling valve
1. Follow all cleaning instructions.
2. Remove the filling tube.
3. Unscrew the filling valve using the mounting tool. Remove the valve with its sealing
washer. Make sure the sealing washer does not remain in the valve block.
4. Rinse the filling valve in the cleaning solution, and clean with soft brush. Should valve
ball stick, push into filling opening of valve with pointed instrument, and release
valve ball.
5. Screw the cleaned/new filling valve together with its sealing washer into the valve
block and tighten it firmly using the mounting tool.
Figure 12
Cleaning/replacing the discharge valve
Figure 13
1. Follow all cleaning instructions.
2. Unscrew the discharge tube.
3. Remove valve locking ring.
4. Unscrew the discharge valve using the mounting tool. Remove the valve with its
sealing washer. Make sure the sealing washer does not remain in the valve block.
5. Rinse the discharge valve in the cleaning solution, and clean with soft brush. Should
valve ball stick, push into filling opening of valve with pointed instrument (e.g.,
disposable pipette tip), and release valve ball.
6. Screw the cleaned/new discharge valve together with its sealing washer into the
valve block and tighten it firmly using the mounting tool.
7. Slide on the valve locking ring making sure the two prongs properly align with the
notches.
9. Autoclaving
This instrument withstands steam sterilization at 121 °C, 2 bar absolute (15 psi) for 20 minutes
in accordance with widely used guidelines. It is the user’s responsibility to ensure effective
autoclaving.

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Preparation prior to the autoclaving
Figure 14
Figure15
Unscrew filling valve (13) and discharge valve (8).
Replace built-in PTFE-rings (12) with the supplied elastic O-rings.
Screw in the filling valve (13) and discharge valve (8) and tighten them firmly using
the mounting tool.
1. Clean the instrument before sterilization.
2. Remove the discharge tube (10) with removed closure cap (11), filling tube (14), and
put all parts on a towel. Avoid contact with hot metal surfaces.
3. Lay the instrument with piston in down position on the towel.
4. Autoclave all parts.
Note:
Allow to cool down in the closed autoclave to avoid deformation through too rapid cooling.
After every autoclaving, inspect all parts for deformities or damage. If necessary, replace them.
Do not reassemble the instrument until it has cooled down to room temperature (cooling time
approx. 2 hours).
Mounting tool can be steam sterilized at 121 °C.
10. Checking the Volume
The accuracy and coefficient of variation of the instrument are determined gravimetrically as
follows:
Set to the nominal volume.
Dispense distilled H2O.
Weigh the dispensed quantity on an analytical balance.
Calculate the dispensed volume taking the temperature into account.
Perform at least 10 dispensing and weighing operations.
Calculate the accuracy (A%) and the coefficient of variation (CV%) by means of the
formula used in statistical quality control. The proceeding is described e.g., in DIN EN
ISO 8655-6. Observe the operating manual of the balance manufacturer and the
corresponding standards.

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Calculations (for the nominal volume)
Mean value 
,
xi= results of weightings
n = number of weightings
Mean volume 
,
Z = correction factor
(e. g. 1.0029 μl/mg at 20 °C, 1013 hPa)
Accuracy
Standard deviation



V0= nominal volume
󰇛󰇜

Coefficient of variation 


11. Trouble Shooting
Problem
Possible cause
Corrective action
Piston difficult to
move
-Formation of
crystals, dirty
-Stop dispensing immediately. Loose
piston by circular motion, but do not
disassemble. Follow all cleaning
instructions.
Filling not
possible
-Volume adjusted to
minimum setting
-Sticking filling valve
-Set to required volume.
-Clean the filling valve. If necessary
replace the valve with sealing washer.
Instrument does
not dispense
liquid
-Discharge tube
closure cap pushed
on
-Discharge tube not
firmly connected or
damaged
-Remove closure cap.
-Push on the closure cap. Hold the
piston in place, and push the original
manufacturer‘s discharge tube on
firmly. Replace a deformed or damaged
discharge tube.
Air bubbles in the
instrument
-Reagent with high
vapor pressure has
been drawn in too
quickly
-Slowly draw in reagent.

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-Seal not inserted,
knurled locking nuts
not firmly connected
-Air not expelled
from the instrument
-Filling tube not
firmly connected or
damaged
-Valves dirty, not
firmly installed or
damaged
-Double check that seal (12) is in place,
and that the knurled locking nuts at the
discharge tube are firmly seated and
finger tight.
-Priming the instrument.
-Push the filling tube on firmly. If
necessary cut off approx. 1 cm of tube
at the upper end and reconnect it or
replace filling tube.
-Cleaning procedure. Tighten the valves
using the mounting tool. If necessary,
replace the valves and sealing washers.
Dispensed
volume is too low
-Discharge tube not
firmly connected or
damaged
-Filling tube not
firmly connected or
damaged
-Valves not firmly
installed or damaged
-Sticking filling valve
-Push the original manufacturer‘s tube
on firmly.
-Cleaning procedure. Push the filling
tube on firmly. If this does not suffice,
cut off approx. 1 cm of tube at the
upper end and re-connect it or replace
filling tube.
-Clean the instrument. Tighten the
valves using the mounting tool, if
necessary replace the valves and
sealing washers.
-Clean or replace discharge tube, if
necessary.
12. Technical Data
Volume
ml
Graduation
ml
A* ≤±
CV*≤
% μl
% μl
0.5 - 5
0.1
0.5 25
0.1 5
1 - 10
0.2
0.5 50
0.1 10
2.5 - 25
0.5
0.5 125
0.1 25
5 - 50
1.0
0.5 250
0.1 50
* Error limits (A = Accuracy, CV = Coefficient of variation) relative to the nominal capacity
(maximum volume) indicated on the instrument, obtained at equal temperature (20 °C) of
instrument, ambience and dist. water, and with smooth operation.
13. Return for Repair
Attention:
For safety reasons only clean/decontaminated instruments can be checked/repaired.
Therefore: Clean and decontaminate the instrument carefully.
Return shipment has to be effected at the risk and cost of the sender.

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