Labcyte Echo 650 Series User manual

This guide provides site preparation information for the Echo® 650 Series Liquid Handler. This includes models 650, 655, 650T
and 655T. The topics below describe the site preparation requirements and instrument specifications for the Echo Liquid
Handlers.
Pre-Installation Requirements
lSite Requirements
lUser Supplied Equipment Requirements
lCommunication Requirements
lAccommodating Your Echo Liquid Handler
lNoise Ratings
lPower Ratings
What You Will Be Receiving
lShipping Dimensions
lEcho Liquid Handler Components
lChiller
lJunair Compressor (Optional and sold separately by Labcyte)
lVacuum Pump (Optional and sold separately by Labcyte)
lClient PC (Sold separately by Labcyte)
Pre-Installation Requirements Verification Form
LABCYTE INC. SITE PREP GUIDE |Echo® 650 Series Liquid Handler
© 2019 LABCYTE INC. All rights reserved.
QSG-2019-1
FEB 2019
Echo® 650 Series Liquid Handler
(Models: 650, 655, 650T, 655T)
SITE PREP GUIDE
Server Version 3.1 | FEBRUARY 2019

Pre-Installation Requirements
The pre-installation requirements are described in the topics below:
lSite Requirements
lUser Supplied Equipment Requirements
lCommunication Requirements
lAccommodating Your Echo Liquid Handler
lNoise Ratings
lPower Ratings
Site Requirements
The following site requirements must be met before the Echo Liquid Handler can be installed:
Operating environment
Temperature: 21°C ± 5°C (70°F± 9°F)
Relative humidity: 10-80%, non-condensing for the Echo Liquid Handler and 35-80% for the
SMC Chiller
ACPower (2 options)
Option 1: 100-120 V, 50/60 Hz, > 10A
Option 2: 200-240 V, 50/60 Hz, > 5A
A minimum of two electrical sockets within 183 cm (6 feet) of the Echo Liquid Handler
supporting a minimum of 800 W are required.
The Echo Liquid Handler and SMC chiller maximum power consumption is 600W and 500W
respectively.
Up to four additional electrical sockets are required for the client PC, monitor, vacuum pump
(optional) and air compressor (optional).
Note: The Echo Liquid Handler power configuration is factory set and requires a Field Service visit in order to be changed
on site.
Compressed air source
Clean, filtered, oil-free, dry air at 552 kPa (80 PSI) minimum, 1034 kPa (150 PSI) maximum
Maximum consumption by Echo Liquid Handler does not exceed 1 cfm at 150 PSI
Note: The Junair OF302-4B oil-less compressor can be purchased from Labcyte (Part #001-12164). For more information,
see the Junair Compressor (Optional and sold separately by Labcyte) topic.
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Vacuum source for source
plate dryer (2 options)
Option 1: House supply. Minimum 200 Torr (266 mbar, 22 in Hg Vac) minimum pumping speed
8.0 m3/hr
Option 2: Standalone vacuum with surge tank (10 L). 100 Torr (133 mbar, 26 in Hg Vac),
minimum pumping speed 2.0 m3/hr
Note: A standalone Vacuubrand MZ 2S-NT vacuum system can be purchased from Labcyte (Part #001-8104). For more
information, see the Vacuum Pump (Optional and sold separately by Labcyte) topic.
Note: The 10 L surge tank comes standard with the Echo Liquid Handler to ensure consistent vacuum.
Instrument mounting surface
(minimal requirements)
The Echo Liquid Handler can emit vibration while in operation and is susceptible to vibration
from other devices. For this reason, the Echo system should be placed on a surface that is
isolated from surrounding equipment or designed to suppress the transmission of vibration
from devices. The surface must be sturdy, level, and water-resistant with cross-bracing or
welded joints.
The surface must be capable of supporting a minimum of 142 kg (313 lbs) weight with
sufficient surface area to accommodate the Echo Liquid Handler in the recommended
operating envelope (see Echo Liquid Handler Dimensions). If the mounting surface is a table
top, the table legs should not rest on casters. If the table has casters, they should be locked.
If the chiller will be installed on the bench surface, it must accommodate an additional 14 kg
(31 lbs).
Destination plate samples At installation, ensure common destination plates to be used with the instrument are
available for tuning the destination plate gripper.
Coupling Fluid Use only ASTM D-1193 Type 1 water in the fluidics system
Coupling Fluid Temperature: 22.1°C ± 0.9°C (71.8°F± 1.6°F)
User Supplied Equipment Requirements
The user must provide the following items:
Hardware
Compressed air fitting compatible with 0.170 in ID / 0.250 in OD tubing between the compressed
air supply and the Echo Liquid Handler tubing (Labcyte provides the compressed air line and
fitting with the Echo Liquid Handler)
Vacuum fitting that connects 3/8 in ID, 5/8 in OD tubing to either house vacuum or a vacuum
pump. The supplied vacuum line is HercoBraid, 3/8 in ID, 5/8 in OD (RyanHerco P/N 0512-072)
Client PC
Minimum Requirements: Microsoft® Windows® 7 using 32- or 64-bit OS, or Microsoft® Windows®
10 64-bit; Intel® Core™ 2 Duo processor or later; 1GB of RAM or greater; 1 GB of available disk
storage; display resolution of 1280 x 1024 (1920x1020 preferred); 2 x Gigabit ethernet ports
Note: Client PC can be purchased directly from Labcyte (Part #ECHO-Client Computer). For information on the Client PC
available from Labcyte, see the Client PC (Sold separately by Labcyte) topic.
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Note: If you are integrating the Echo Liquid Handler into an automated system and need to install the Client + Server
software onto a workstation, it must contain an Intel Core™ i5 processor or better.
Note: If you are setting up an Echo 650T or 655T Liquid Handler that will be using tubes, a capper/decapper is required
before Labcyte Service and Support can install or service the instrument.
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Communication Requirements
The user environment must support the following communication requirements:
Server connection Echo Liquid Handling Platform (Server Version 3.1.x)
Network connection 10/100/1000 BaseT
Network protocol TCP/IP
User account Labcyte (The client PC must have an account, with administrator privileges, that is accessible by
Labcyte Service and Support to service the Echo Liquid Handler system.)
Accommodating Your Echo Liquid Handler
The user must set aside the space to accommodate Echo Liquid Handler installation.
The Echo Liquid Handler
Dimensions 53.8 cm width x 74.6 cm depth x 91.5 cm height (21.2 x 29.3 x 36.0 inches)
Echo Liquid Handler working
envelope (minimal installation
requirements)
Top: It is recommended to have 25.4 cm (10 in) above the instrument to remove the top cover
for servicing.
Front: It is recommended to have 40.6 cm (16 in) to allow sufficient clearance to load and
unload microplates by hand or by robot.
Side: It is recommended to have 121.9 cm (48 in) minimum on left and right sides to allow for
serviceability. The Echo Liquid Handler should have sufficient space to be rotated, otherwise
it will need to be removable for service.
Rear: It is recommended to have 30.5 cm (12 in) minimum behind the instrument to allow for
cables, hoses, tubing, and maintenance access. The coupling fluid bottle and optional caddy
fit within the rear clearance but can be relocated if necessary.
SMCChiller Dimensions
30 cm plus fittings width x 34 cm Depth x 18 cm Height (11.8 in x 13.4 in x 36.4 in). It is
recommended that the chiller be installed within 121.9 cm (48 inches) of the Echo Liquid
Handler.
Echo Client PC Additional space for the client PC, monitor, keyboard and mouse.
Other
Space must be allocated for:
lAny robotics in the system
lWorkspace for microplates
Note: The instrument can be installed with a minimum of 2.5 cm (1 in) at the top if there is sufficient space to lift the top
and slide it all the way forward. Talk to your Labcyte service representative for additional details.
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Figure 1: Instrument Layout (Front View, Top View, Side View)
Noise Ratings
The noise ratings for the Echo Liquid Handler are below:
Noise Rating 64 dBA
Power Ratings
The power ratings for the Echo Liquid Handler are below:
Power Rating 600W without the chiller
Heat Rating 600W without the chiller
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

What You Will Be Receiving
Shipping Dimensions
The Echo Liquid Handler System is shipped in a crate with the following dimensions:
Instrument shipping crate weight 278 kg (577 lbs)
Instrument shipping crate
dimensions 114.3 cm width x 99.1 cm depth x 124.8 cm height (45 x 39 x 49.1 inches)
Accessory crates
Additional devices and/or consumables purchased together with the Echo Liquid
Handler may ship in the crate with the Echo Liquid Handler if there is sufficient room or
separately.
Echo Liquid Handler Components
The following components are included with the Echo Liquid Handler:
Vacuum line HercoBraid, 9.5 mm (3/8 in) ID, 9.5 mm (5/8 in) OD (RyanHerco P/N 0512-072)
HFCD 17612 coupling body (Colder Products)
Vacuum Surge Tank
Assembly (Optional) Usage dependent on configuration, but included with the Echo (P/N 001-5150)
Compressed air line
4.6 meters (15 ft) of polyurethane tubing; 6.35 mm (0.25 in) OD, 4.24 mm (0.170 in) ID (SMC
Pneumatics P/N TIUB07-BU)
LCD 13004 coupling body (Colder Products).
Coupling fluid bottle 1 L (P/N 001-5438)
Accessory kit Includes tubing and power cord for connecting the chiller (P/N 001-15272)
SMC chiller P/N 001-11149
Ethernet cable Crossover, 3 meters (10 ft)
Software Echo Client Utility installer provided on CD or USB drive
AC Power, Configuration, and Fuses
The following table describes the AC Power and Fuse requirements for the Echo Liquid Handler:
Voltage Frequency Power Fuse Part Number
100-120 V 50 Hz 8 A 250 VAC, 10 A, FAST 001-3966
200–240 V 50 Hz 5 A 250 VAC, 5 A, FAST 001-3967
Table 1: Electrical Configurations for the Echo Liquid Handler
Note: The power cord may need to be replaced with one conforming to local AC power receptacles. To determine which
AC power configuration you have, see the Power and Fuse Rating Label on the back panel of the Echo Liquid Handler.
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Chiller
The SMC chiller (P/N 001-11149) is included with your Echo system. The SMC chiller utilizes a stainless steel thermoelectric (Peltier)
heat exchanger that regulates fluid temperature. The SMC chiller ships configured for operation with Echo Liquid Handlers:
Power Supply
The SMC chiller uses an auto switching power supply capable of running any single-phase
AC voltage in the range 100-240V, 50-60 Hz
A power cord suitable for the install location is provided
Maximum power is 500W
Temperature Control
The SMC chiller controls the temperature to +/- 0.02 °C by cooling and heating as
appropriate
The SMC chiller is air cooled
Ambient Operating Conditions
Temperature: 10-35 °C
Relative Humidity: 35-80%
Figure 2: SMCChiller
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Junair Compressor (Optional and sold separately by Labcyte)
The Echo Liquid Handler requires a compressed air source that provides clean, filtered, oil-free, dry air at 552 kPa (80 PSI) minimum
and 1034 kPa (150 PSI) maximum. The Junair OF302-4B oil-less compressor can be purchased from Labcyte (Part # varies
dependent on geography.)
The user must also provide a fitting between the compressed air supply and the Echo Liquid Handler. Labcyte provides the
compressed air line and fitting with the Echo Liquid Handler, but the customer must provide a source compatible with 170 in
ID/.250 in OD tubing.
The table below lists the specifications for the Junair OF302-4B compressor:
Voltage V 100 100 120 200 200 230 230 3x400 3x440
Frequency Hz 50 60 60 50 60 50 60 50 60
Motor
HP 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60 0.60
kW 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44 0.44
Displacement
I/min 108 138 138 108 138 108 138 108 138
CFM 3.81 4.87 4.87 3.81 4.87 3.81 4.87 3.81 4.87
FAD @ 8 bar
l/min 38 44 44 38 44 38 44 38 44
CFM 1.34 1.55 1.55 1.34 1.55 1.34 1.55 1.34 1.55
Maximum
pressure
bar 8 8 8 8 8 8 8 8 8
psi 120 120 120 120 120 120 120 120 120
Maximum current A 7.5 8.7 6.6 4.1 4.6 3.4 4.1 1.4 1.4
Tank size
liters 4 4 4 4 4 4 4 4 4
gallon 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1 1.1
Weight
kg 22 22 22 22 22 22 22 22 22
lbs 49 49 49 49 49 49 49 49 49
Noise level dB
(A)/lm 65 66 66 65 66 65 66 65 66
Thermal
protection Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes
Duty cycle 100% 100% 100% 100% 100% 100% 100% 100% 100% 100%
Note: Maximum consumption by Echo Liquid Handler for instrument pneumatics does not exceed 1 cfm at 150 PSI.
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Figure 3: Junair OF302-4B oil-less compressor
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Vacuum Pump (Optional and sold separately by Labcyte)
The Echo Liquid Handler requires a vacuum source. The vacuum dries source plates before they are unloaded. This requirement
can be met through a house vacuum or a vacuum pump.
The vacuum source should have the following specifications:
House supply Minimum 200 Torr (266 mbar, 22 in Hg Vac) minimum pumping speed 8.0
m3/hr
Standalone vacuum with surge tank (10 L) 100 Torr (133 mbar, 26 in Hg Vac), minimum pumping speed 2.0 m3/hr
Customers must provide a fitting that connects the vacuum source and the Echo Liquid Handler vacuum line. The supplied
vacuum line is HercoBraid, 9.5 mm (3/8 in) ID, 9.5 mm (5/8 in) OD (RyanHerco P/N 0512-072).
A standalone Vacuubrand MZ 2S-NT vacuum system can be purchased from Labcyte (Part #001-8104). This vacuum is a dual
voltage/cycle compatible system.
The table below lists the specifications for the Vacuubrand MZ 2S-NT vacuum system:
Permissible ambient temperature
storage/operation
14 to 140/50 - 104 F°
-10 to +60/ +10 - +40 C°
Permissible relative atmospheric moisture during
operation (no condensation) 30 to 85%
No-load speed 50/60 hZ 1500/1800 rpm
Device fuse Slow blow fuse 6.3 A
Maximum pumping speed 50/60 Hz 1.3/1.4 cfm
2.2/2.4 m3/h
Ultimate vacuum (absolute) 5.2 Torr
7 mbar
Maximum permissible outlet pressure (absolute) 29 psi
2 bar
Maximum permissible different between inlet and
outlet
29 psi
2 bar
Rated motor power 0.24 hp
0.18 kW
Maximum permissible range of supply voltage
(+10%)
100-115 V ~50/60 Hz
120 V ~ 60 Hz
230 V ~50/60 Hz
Dual voltage motor
100-115 V ~50/60 Hz
120 V ~ 60 Hz
200- 230 V ~50/60 Hz
Maximum rated current at:
100-115 V ~50/60 Hz, 120 V ~ 60 Hz
200- 230 V ~50/60 Hz, 230 V ~50/60 Hz
3 A
1.8 A
Inlet Hose nozzle for tubing I.D. 3/8 in
Hose nozzle DN 10 mm
Outlet Silencer / G1/4 in
Dimensions L X W X H (approximate) 9.6 X 9.4 X 7.8 in
243 X 239 X 198 mm
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Weight (approximate) 24.2 lbs
11 kg
Figure 4: Vacuubrand MZ 2S-NT vacuum system
Client PC (Sold separately by Labcyte)
Client PC specifications (Dell OptiPlex XE2):
Operating system Microsoft® Windows 7 32-bit OS
CPU Intel® Core™ XE2 i5-4570S 2.9 GHz Processor
Memory 4GB RAM or greater
Hard drive storage 1 TB hard drive
Video Resolution 1280 x 1024 resolution
Ethernet 2 Gigabit 10/100/1000 (Two Ethernet Cards)
Included Accessories:
lMicrosoft® Office 2016
lMonitor, 22 in, DVI-D/DP/VGA
lDell USB Keyboard, Black
lDell MS111 USB Optical Mouse, OptiPlex and Fixed Precision
lLabcyte Mousepad
Software specifications:
Optionally, the following Echo software applications are available for purchase:
lEcho Plate Reformat
lEcho Dose-Response
lEcho Cherry Pick
lEcho Array Maker
lEcho Plate Audit
lEcho Combination Screen
Note: Echo 650 Series Liquid Handlers require Echo software applications version 1.7 or higher.
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SITE PREP GUIDE | Echo® 650 Series Liquid Handler PN | 001-16480

Pre-Installation Requirements Verification Form
Please send this Pre-Installation Requirement Verification Form to the local Labcyte representative assigned to the installation.
Verification of the pre-installation requirements is required before the service representative will schedule the installation.
Verified Requirement Description Yes No
Operating environment Are the temperature and relative humidity operating environment
requirements met?
Sturdy, level, water-resistant
bench top
Is there a sturdy, level, water-resistant bench available for the Echo
6XX series instrument?
Operating space envelope Does the space available for the instrument meet the operating
space envelope requirement?
AC power Is AC power meeting the AC power specifications and located within
6 feet of the installation location?
Compressed air source Is there a source of compressed air meeting the compressed air
source specification?
Vacuum source Is there a vacuum source meeting the vacuum specification?
Client PC Is there a client PC available for the installation meeting the client
PC specification?
Destination plates Are commonly used destination plates available for tuning the
destination plate gripper at installation?
One installation visit is provided with the purchase of an Echo 650 Series Liquid Handler instrument. If the
pre-installation requirements are not met at the time of installation, it may delay the installation and require an additional visit.
If you have any questions regarding the lab environment setup or required equipment, please contact your local Labcyte
Representative or email [e-mail].
LABCYTE INC. SITE PREP GUIDE |Echo® 650 Series Liquid Handler
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. QSG-2019-1
FEB 2019
LABCYTE INC.
170 Rose Orchard Way
San Jose, CA 95134, USA
Toll-free: +1 877 742−6548
Fax: [phone]−2010
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All product names and brands are properties of their respective owners.
© 2019 Labcyte Inc. All rights reserved. Labcyte®, Echo®, MicroClime®, the Labcyte logo, and Access™ are
registered trademarks or trademarks ofLabcyte Inc., in the U.S. and/or other countries.
Echo® Liquid Handlers, Applications Software and Automation Systems from Labcyte Inc. are covered by one or more of the following patents:
United States: 6,416,164; 6,548,308; 6,603,118; 6,612,686; 6,642,061; 6,666,541; 6,707,038; 6,710,335; 6,746,104; 6,802,593; 6,808,934; 6,809,315; 6,849,423; 6,855,925; 6,869,551;
6,893,836; 6,893,115; 6,916,083; 6,932,097; 6,938,987; 6,938,995; 6,991,917; 7,070,260; 7,090,333; 7,185,969; 7,270,986; 7,354,141; 7,405,072; 7,405,395; 7,439,048; 7,454,958;
7,481,511; 7,717,544; 7,899,645; 7,900,505; 7,901,039; 8,107,319, 8,389,295; 8,503,266; 8,770,69; 9,212,250; 9,586,215; 9,908,133; 10,112,212; 10,118,186. European Patent EP: 1322430;
1324823; 1337325; 1352112; 1366356; 1348116, 1409254, 1534526; 1585636; 1879697. Japan: 4189964; 4309131; 4434581; 4559218; 4624644; 4990476; 4955901; 4990476; 5031875.
Additional patents are approved and pending in the United States and other countries.