Labnet Enduro E0303 User manual

Instruction Manual
250 Volt
Electrophoresis Power Supply
A high current power supply for SDS-PAGE and DNA/RNA
electrophoresis applications
Catalog Number
110 volt: E0303
230 volt: E0303-230V
Copyright Nov., 2007
Version # 1

2
Table of Contents
FCC Warning 3
Safety Information 3
Package Contents 4
Product Specifications 5
Overview 6
escription of Buttons and Switches 7
Getting Started 8
Important Guidelines 8
Operation 9
Choosing Limiting Parameter Settings 11
Troubleshooting 12
Operational Electrical Parameters 13
Common Errors 15
Repair and Maintenance of EN URO 250V Power
Supply
16
Technical Service 17
Warranty 17
Equipment isposal 18

3
Warning
Federal Communications Commission Advisory
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio frequency energy and, if
not installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications. peration of this equipment in a residential area is likely
to cause harmful interference in which case the user will be required to correct the interference
at their expense. Changes or modifications not expressly approved by the party responsible
for compliance could void the user’s authority to operate the equipment.
Safety Information
Avoiding Electrical Shock
The ENDUR 250 Volt Power Supply produces up to 3 A or 250 voltage outputs which are
electrically isolated from ground to reduce the risk of electrical shock to the user. Follow the
guidelines below to ensure safe operation of the unit.
The ENDUR 250 Volt Power Supply has been designed for use with electrophoresis cells
with shielded banana plugs thus minimizing any potential shock hazard to the user. Labnet
recommends against the use of unshielded banana plugs.
To avoid electrical shock:
1. NEVER connect or disconnect wire leads from the power jacks when the red indicator light
at the Start/Stop key is on or when “RUNNING” is displayed on the screen.
2. WAIT at least 5 seconds after stopping a run before handling output leads or connected
apparatus.
3. ALWAYS make sure that hands, work area, and instruments are clean and dry before
making any connections or operating the power supply.
4. NLY connect the power supply to a properly grounded AC outlet.

4
Avoiding Damage to the Instrument
1. For proper ventilation, leave at least 10 cm of space behind the instrument, and at least 5
cm of space on each side.
2. Do not operate the power supply in high humidity environments (> 95%), or where
condensation may occur.
3. To avoid condensation after operating the power supply in a cold room, wrap the unit in a
plastic bag and allow at least 2 hours for the unit to equilibrate to room temperature before
removing the bag and operating the unit.
Symbols
Used on the ENDUR 250 Volt Power Supply to indicate an area where a
potential shock hazard may exist.
Product Contents
Types of Products
This manual is supplied with the following products:
ENDUR 250 Volt Power Supply (115 / 230 VAC switchable, 50/60 Hz)
Product Contents
Component Quantity
250V Power Supply 1 each
Instruction Manual 1 each
Extra Fuse 1 each
Power Cord 1 each
Warranty Card 1 each
pon Receiving the Instrument
Examine the unit carefully for any damage incurred during transit. Any damage claims must be
filed with the carrier, keep the supplied box for inspection. The warranty does not cover
in-transit damage.
To ensure safe, reliable operation, always operate the ENDUR 250 Volt Power
Supply in accordance with the manufacturer’s instructions. Always wear
protective gloves and safety glasses when working in a laboratory environment.

5
Product Specifications
END RO 250 Volt Power Supply Specifications
Input Power (Switchable) 115 VAC, 50-60 Hz
230 VAC, 50-60 Hz
Fuses ne 4A/250V, one extra fuse is provided
Output power max 300 watts
Output voltage range 5~250V
Output current range 10 mA~3 A
Timer ~99.99 per step
Terminal pairs 4: 4 positive voltage and 4 negative voltage
Operating Modes
Constant Voltage 1V step
Constant Current 1 mA step
Constant Power 1 watt step
Crossover Auto
isplay type LCD Graphic type
isplay size 53.64 x 15.64 mm (W x H)
Pause function Yes
Safety feature No Load Detection
Load Change Detection
verload detection
Ground Leak
Auto Restart
Programmable Yes
Store file no.: 10
Program: up to 10 steps
Stackable Yes
Housing material Flame retardant ABS
Housing size 200 x 290 x 70 mm (W x D x H)
Operating temp. 0-40°C
Operating environment 100% RH, 75 KPa-106 Kpa, Altitude not to exceed 2000 meters
Weight 2.6 kg
Certifications CE; TUV; UL
Warranty 3 years

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Introduction
Overview
The ENDUR 250 Volt Power Supply is a microprocessor-controlled power supply designed
to meet most electrophoresis needs in a single easy to use unit. The power supply’s small
foot print and stacking feature conserve valuable bench space. The power supply is capable of
running constant voltage, constant current or constant power applications and programming
mode concurrently. This instrument is ideal for DNA/RNA electrophoresis, SDS-PAGE, native
PAGE, and IEF applications. With four sets of output jacks that can be used simultaneously,
the ENDUR 250 Volt Power Supply is designed to run applications at maximal power and
efficiency. The ENDUR 250 Volt Power Supply offers three modes, Constant Voltage,
Constant Current and Constant Wattage Mode. This manual describes the setup and
operation of ENDUR 250 Volt Power Supply including important information on safety and
maintenance of the unit.
Features of the END RO 250 Volt Power Supply
Important features of the ENDUR 250 Volt Power Supply are listed below:
• Constant Voltage, Power or Current
• Capable of running multiple electrophoresis units
• Large LCD display with clear menu prompts for easy use
• Programming capabilities for limiting voltage (V), current (mA), power (W).
• Four sets of output terminals
• Capability to specify run durations by time or volt-hours
Purpose of the Manual
This manual includes the following information:
• Safety information
• Instructions for setting up the instrument
• perating instructions.
• Guidelines for repair and maintenance
Follow the recommendations and guidelines provided in this manual for your safety, best
results, and optimal performance of your ENDUR 250 Volt Power Supply.

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Operational keys
Key Functions
STOP key: Used to stop operation from the Running Screen
START/PAUSE key: Used to start operation / temporarily interrupt power to
an operation in progress without terminating electrophoresis and to resume
power after pausing without resetting the timer.
CONSTANT key: Used to set up constant voltage or current values
MO E key: Used to choose either Constant Voltage, Constant Current
or Constant Wattage mode
own Arrow key: Used to move cursor down between parameters and to
decrease numeric values
Up Arrow key: Used to move cursor up between parameters and to increase
numeric values
Getting Started
Installing the END RO 250 Volt Power Supply
1. Check the label located near the AC inlet to ensure that the unit is compatible with locally
provided voltage.
2. Place the ENDUR 250 Volt Power Supply on a level laboratory bench. Keep the area
around the power supply clear to ensure proper ventilation of the unit.
3. For your safety: Position the unit properly such that the On-Off switch and the AC inlet
located on the rear of the unit are easily accessible.
4. Ensure the AC power switch is in the Off position.
5. Attach the power cord to the AC inlet. Use only properly grounded AC outlets and power
cords.
6. Connect the leads from the electrophoresis unit; insert the red lead (+) into the red output
jack, and the black lead (-) into the black output jack.
MODE
CONSTANT
START
PAUSE
STOP

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Important Guidelines
Introduction
The important guidelines for operating the ENDUR 250 Volt Power Supply are provided in
this section. We recommend that you carefully review these guidelines before operating the
instrument.
Important
For best results, do NOT use the ENDUR 250 Volt Power Supply at its maximum electrical
load limits. Variations in buffer conditions can result in exceeding the power supply’s maximum
voltage, current, or power output capacity and produce undesirable variations in
electrophoretic separations.
General Operating Instructions
Follow the instructions below to operate the ENDUR 250 Volt Power Supply.
• Turn on the ENDUR 250 Volt Power Supply using the power switch on the rear of the
instrument. Upon start-up, the current settings appear on the screen.
• Use the START/PAUSE and STOP keys and output jacks for applications.
• Use the CONSTANT key to set up operation mode.
Recommendation
The duration of electrophoresis can be defined in time (hours/minutes). When using this or any
electrophoresis product, we recommend that you adhere to the protocols given in the
electrophoresis product manuals, and durations, specified in time.
Important
For best results, follow these important guidelines when running multiple gels and
electrophoresis units concurrently.
For example:
• Avoid running samples of widely differing salt concentrations or sample buffers at the same
time or on the same gel.
• Properly prepare and desalt your samples.
Note: Variations in conductivity due to differences in buffer salt concentrations can affect the run of all the
samples run at the same time.

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Operational Modes
Introduction
The ENDUR 250 Volt Power Supply is designed to operate under different modes, Constant
mode or Programming Mode, depending upon your electrophoresis needs.
Use the Constant Voltage, Constant Power or Constant Current Operation for applications that
require only one specific voltage limit, current limit, and power limit continuously during the
entire duration of electrophoresis.
Start-up
n power up use the ▼▲ arrow key to choose the operational Mode (Constant Mode or
Program Mode) , then press Mode to select..
• n the isplay Screen:
• The controlling value (Volts, Amp or Watts) is displayed on the left-top side of the
display screen.
• The Timer is the first line on the right-top, and the other values (Volts, Amp or Watts)
are displayed in the second and third line on the right side of the display screen.
Constant Operation Protocol
Introduction
Instructions for operating the ENDUR 250 Volt Power Supply in the Constant Operation are
provided in this section. The Constant Voltage, Current, or Power Mode allow you to specify
a voltage limit, current limit and power limit to be used during the entire duration of
electrophoresis. Review the guidelines provided in this manual before starting
electrophoresis using the ENDUR 250 Volt Power Supply.
A basic Constant Voltage, Current Mode, or Constant Power operating procedure of the
ENDUR 250 Volt Power Supply is provided below. We recommend reading the guidelines
provided in this manual for best results before starting an operation.
1. Use the power switch on the rear side of the instrument to turn on the ENDUR 250 Volt
Power Supply. n Display Screen Constant Mode and Program Mode will appear.
2. With the Constant Mode highlighted press the Mode key
3. Use the Mode Key to navigate to needed parameter (Volt. Amp, or Wat)
4. Press the CONSTANT key to select, Constant Voltage Operation, Constant Current
Operation or Constant Power Operation from the isplay Screen.
5. Use the ▼▲ arrow keys to set either voltage (V LT)) or current (AMP) or Power (WAT) to
the appropriate values.
6. Use the Mode key to select TIME and use ▼▲ arrow key to set the time (hours/minutes) to
specify the duration of the electrophoresis.

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7. Press START/PAUSE key to start electrophoresis.
8. Press the START/PAUSE key again to temporarily interrupt power to ongoing
electrophoresis without terminating the operation along with LED flashing.
9. Press the STOP key to stop electrophoresis.
10. If the parameters need to be changed during the run you must stop electrophoresis by
pressing the Mode key. Enter the changes and then press Start/Stop once again to restart
your operation.
Note: After stopping and restarting an operation, the timer resets to selected time and does not take into
account the time that electrophoresis was in progress before it was stopped.
Basic Programming Protocol
Introduction
Instructions for operating the ENDUR 250 Volt Power Supply in the Programming Mode are
provided in this section. The Programmable Mode allows you to vary levels in voltage (V),
current (mA), and power (W) during specified periods of time as discrete changes (STEP) for
up to 10 steps, depending upon your electrophoresis needs.
A basic Programmable Mode operating procedure of the ENDUR 250 Volt Power Supply is
provided below. We recommend reading the guidelines provided in this manual for best results
before starting an operation.
Selecting a program
1. Select Program Mode by scrolling down using the ▼ key
2. When highlighted press Mode key
3. Select file number using the a▼▲ arrow keys
4. When file number is located press the Mode key
5. Select the number of steps by using the arrow ▼ ▲ arrow keys
6. To enter the parameters of the run press the Mode key
7. Voltage will appear on the display to enter voltage press Mode key
8. To change voltage, use the ▼ ▲ arrow keys.
9. Select Amperage or Watts by pressing the Mode key until desired parameter is
flashing.
10. Increase or decrease the value by using the▼▲ arrow keys
11. To set the time in hours press the Mode key
12. Increase or decrease the value by using the▼▲ arrow keys
13. Select the Mode key again to select minutes.
14. Increase or decrease the value by using the▼▲ arrow keys
15. Repeat 6 thru 14 above to program successive steps.