Labnet Enduro E0303 User manual

Instruction Manual
250 Volt
Electrophoresis Power Supply
A high current power supply for SDS-PAGE and DNA/RNA
electrophoresis applications
Catalog Number
110 volt: E0303
230 volt: E0303-230V
Copyright Nov., 2007
Version # 1

2
Table of Contents
FCC Warning 3
Safety Information 3
Package Contents 4
Product Specifications 5
Overview 6
escription of Buttons and Switches 7
Getting Started 8
Important Guidelines 8
Operation 9
Choosing Limiting Parameter Settings 11
Troubleshooting 12
Operational Electrical Parameters 13
Common Errors 15
Repair and Maintenance of EN URO 250V Power
Supply
16
Technical Service 17
Warranty 17
Equipment isposal 18

3
Warning
Federal Communications Commission Advisory
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio frequency energy and, if
not installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications. peration of this equipment in a residential area is likely
to cause harmful interference in which case the user will be required to correct the interference
at their expense. Changes or modifications not expressly approved by the party responsible
for compliance could void the user’s authority to operate the equipment.
Safety Information
Avoiding Electrical Shock
The ENDUR 250 Volt Power Supply produces up to 3 A or 250 voltage outputs which are
electrically isolated from ground to reduce the risk of electrical shock to the user. Follow the
guidelines below to ensure safe operation of the unit.
The ENDUR 250 Volt Power Supply has been designed for use with electrophoresis cells
with shielded banana plugs thus minimizing any potential shock hazard to the user. Labnet
recommends against the use of unshielded banana plugs.
To avoid electrical shock:
1. NEVER connect or disconnect wire leads from the power jacks when the red indicator light
at the Start/Stop key is on or when “RUNNING” is displayed on the screen.
2. WAIT at least 5 seconds after stopping a run before handling output leads or connected
apparatus.
3. ALWAYS make sure that hands, work area, and instruments are clean and dry before
making any connections or operating the power supply.
4. NLY connect the power supply to a properly grounded AC outlet.

4
Avoiding Damage to the Instrument
1. For proper ventilation, leave at least 10 cm of space behind the instrument, and at least 5
cm of space on each side.
2. Do not operate the power supply in high humidity environments (> 95%), or where
condensation may occur.
3. To avoid condensation after operating the power supply in a cold room, wrap the unit in a
plastic bag and allow at least 2 hours for the unit to equilibrate to room temperature before
removing the bag and operating the unit.
Symbols
Used on the ENDUR 250 Volt Power Supply to indicate an area where a
potential shock hazard may exist.
Product Contents
Types of Products
This manual is supplied with the following products:
ENDUR 250 Volt Power Supply (115 / 230 VAC switchable, 50/60 Hz)
Product Contents
Component Quantity
250V Power Supply 1 each
Instruction Manual 1 each
Extra Fuse 1 each
Power Cord 1 each
Warranty Card 1 each
pon Receiving the Instrument
Examine the unit carefully for any damage incurred during transit. Any damage claims must be
filed with the carrier, keep the supplied box for inspection. The warranty does not cover
in-transit damage.
To ensure safe, reliable operation, always operate the ENDUR 250 Volt Power
Supply in accordance with the manufacturer’s instructions. Always wear
protective gloves and safety glasses when working in a laboratory environment.

5
Product Specifications
END RO 250 Volt Power Supply Specifications
Input Power (Switchable) 115 VAC, 50-60 Hz
230 VAC, 50-60 Hz
Fuses ne 4A/250V, one extra fuse is provided
Output power max 300 watts
Output voltage range 5~250V
Output current range 10 mA~3 A
Timer ~99.99 per step
Terminal pairs 4: 4 positive voltage and 4 negative voltage
Operating Modes
Constant Voltage 1V step
Constant Current 1 mA step
Constant Power 1 watt step
Crossover Auto
isplay type LCD Graphic type
isplay size 53.64 x 15.64 mm (W x H)
Pause function Yes
Safety feature No Load Detection
Load Change Detection
verload detection
Ground Leak
Auto Restart
Programmable Yes
Store file no.: 10
Program: up to 10 steps
Stackable Yes
Housing material Flame retardant ABS
Housing size 200 x 290 x 70 mm (W x D x H)
Operating temp. 0-40°C
Operating environment 100% RH, 75 KPa-106 Kpa, Altitude not to exceed 2000 meters
Weight 2.6 kg
Certifications CE; TUV; UL
Warranty 3 years

6
Introduction
Overview
The ENDUR 250 Volt Power Supply is a microprocessor-controlled power supply designed
to meet most electrophoresis needs in a single easy to use unit. The power supply’s small
foot print and stacking feature conserve valuable bench space. The power supply is capable of
running constant voltage, constant current or constant power applications and programming
mode concurrently. This instrument is ideal for DNA/RNA electrophoresis, SDS-PAGE, native
PAGE, and IEF applications. With four sets of output jacks that can be used simultaneously,
the ENDUR 250 Volt Power Supply is designed to run applications at maximal power and
efficiency. The ENDUR 250 Volt Power Supply offers three modes, Constant Voltage,
Constant Current and Constant Wattage Mode. This manual describes the setup and
operation of ENDUR 250 Volt Power Supply including important information on safety and
maintenance of the unit.
Features of the END RO 250 Volt Power Supply
Important features of the ENDUR 250 Volt Power Supply are listed below:
• Constant Voltage, Power or Current
• Capable of running multiple electrophoresis units
• Large LCD display with clear menu prompts for easy use
• Programming capabilities for limiting voltage (V), current (mA), power (W).
• Four sets of output terminals
• Capability to specify run durations by time or volt-hours
Purpose of the Manual
This manual includes the following information:
• Safety information
• Instructions for setting up the instrument
• perating instructions.
• Guidelines for repair and maintenance
Follow the recommendations and guidelines provided in this manual for your safety, best
results, and optimal performance of your ENDUR 250 Volt Power Supply.

7
Operational keys
Key Functions
STOP key: Used to stop operation from the Running Screen
START/PAUSE key: Used to start operation / temporarily interrupt power to
an operation in progress without terminating electrophoresis and to resume
power after pausing without resetting the timer.
CONSTANT key: Used to set up constant voltage or current values
MO E key: Used to choose either Constant Voltage, Constant Current
or Constant Wattage mode
own Arrow key: Used to move cursor down between parameters and to
decrease numeric values
Up Arrow key: Used to move cursor up between parameters and to increase
numeric values
Getting Started
Installing the END RO 250 Volt Power Supply
1. Check the label located near the AC inlet to ensure that the unit is compatible with locally
provided voltage.
2. Place the ENDUR 250 Volt Power Supply on a level laboratory bench. Keep the area
around the power supply clear to ensure proper ventilation of the unit.
3. For your safety: Position the unit properly such that the On-Off switch and the AC inlet
located on the rear of the unit are easily accessible.
4. Ensure the AC power switch is in the Off position.
5. Attach the power cord to the AC inlet. Use only properly grounded AC outlets and power
cords.
6. Connect the leads from the electrophoresis unit; insert the red lead (+) into the red output
jack, and the black lead (-) into the black output jack.
MODE
CONSTANT
START
PAUSE
STOP

8
Important Guidelines
Introduction
The important guidelines for operating the ENDUR 250 Volt Power Supply are provided in
this section. We recommend that you carefully review these guidelines before operating the
instrument.
Important
For best results, do NOT use the ENDUR 250 Volt Power Supply at its maximum electrical
load limits. Variations in buffer conditions can result in exceeding the power supply’s maximum
voltage, current, or power output capacity and produce undesirable variations in
electrophoretic separations.
General Operating Instructions
Follow the instructions below to operate the ENDUR 250 Volt Power Supply.
• Turn on the ENDUR 250 Volt Power Supply using the power switch on the rear of the
instrument. Upon start-up, the current settings appear on the screen.
• Use the START/PAUSE and STOP keys and output jacks for applications.
• Use the CONSTANT key to set up operation mode.
Recommendation
The duration of electrophoresis can be defined in time (hours/minutes). When using this or any
electrophoresis product, we recommend that you adhere to the protocols given in the
electrophoresis product manuals, and durations, specified in time.
Important
For best results, follow these important guidelines when running multiple gels and
electrophoresis units concurrently.
For example:
• Avoid running samples of widely differing salt concentrations or sample buffers at the same
time or on the same gel.
• Properly prepare and desalt your samples.
Note: Variations in conductivity due to differences in buffer salt concentrations can affect the run of all the
samples run at the same time.

9
Operational Modes
Introduction
The ENDUR 250 Volt Power Supply is designed to operate under different modes, Constant
mode or Programming Mode, depending upon your electrophoresis needs.
Use the Constant Voltage, Constant Power or Constant Current Operation for applications that
require only one specific voltage limit, current limit, and power limit continuously during the
entire duration of electrophoresis.
Start-up
n power up use the ▼▲ arrow key to choose the operational Mode (Constant Mode or
Program Mode) , then press Mode to select..
• n the isplay Screen:
• The controlling value (Volts, Amp or Watts) is displayed on the left-top side of the
display screen.
• The Timer is the first line on the right-top, and the other values (Volts, Amp or Watts)
are displayed in the second and third line on the right side of the display screen.
Constant Operation Protocol
Introduction
Instructions for operating the ENDUR 250 Volt Power Supply in the Constant Operation are
provided in this section. The Constant Voltage, Current, or Power Mode allow you to specify
a voltage limit, current limit and power limit to be used during the entire duration of
electrophoresis. Review the guidelines provided in this manual before starting
electrophoresis using the ENDUR 250 Volt Power Supply.
A basic Constant Voltage, Current Mode, or Constant Power operating procedure of the
ENDUR 250 Volt Power Supply is provided below. We recommend reading the guidelines
provided in this manual for best results before starting an operation.
1. Use the power switch on the rear side of the instrument to turn on the ENDUR 250 Volt
Power Supply. n Display Screen Constant Mode and Program Mode will appear.
2. With the Constant Mode highlighted press the Mode key
3. Use the Mode Key to navigate to needed parameter (Volt. Amp, or Wat)
4. Press the CONSTANT key to select, Constant Voltage Operation, Constant Current
Operation or Constant Power Operation from the isplay Screen.
5. Use the ▼▲ arrow keys to set either voltage (V LT)) or current (AMP) or Power (WAT) to
the appropriate values.
6. Use the Mode key to select TIME and use ▼▲ arrow key to set the time (hours/minutes) to
specify the duration of the electrophoresis.

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7. Press START/PAUSE key to start electrophoresis.
8. Press the START/PAUSE key again to temporarily interrupt power to ongoing
electrophoresis without terminating the operation along with LED flashing.
9. Press the STOP key to stop electrophoresis.
10. If the parameters need to be changed during the run you must stop electrophoresis by
pressing the Mode key. Enter the changes and then press Start/Stop once again to restart
your operation.
Note: After stopping and restarting an operation, the timer resets to selected time and does not take into
account the time that electrophoresis was in progress before it was stopped.
Basic Programming Protocol
Introduction
Instructions for operating the ENDUR 250 Volt Power Supply in the Programming Mode are
provided in this section. The Programmable Mode allows you to vary levels in voltage (V),
current (mA), and power (W) during specified periods of time as discrete changes (STEP) for
up to 10 steps, depending upon your electrophoresis needs.
A basic Programmable Mode operating procedure of the ENDUR 250 Volt Power Supply is
provided below. We recommend reading the guidelines provided in this manual for best results
before starting an operation.
Selecting a program
1. Select Program Mode by scrolling down using the ▼ key
2. When highlighted press Mode key
3. Select file number using the a▼▲ arrow keys
4. When file number is located press the Mode key
5. Select the number of steps by using the arrow ▼ ▲ arrow keys
6. To enter the parameters of the run press the Mode key
7. Voltage will appear on the display to enter voltage press Mode key
8. To change voltage, use the ▼ ▲ arrow keys.
9. Select Amperage or Watts by pressing the Mode key until desired parameter is
flashing.
10. Increase or decrease the value by using the▼▲ arrow keys
11. To set the time in hours press the Mode key
12. Increase or decrease the value by using the▼▲ arrow keys
13. Select the Mode key again to select minutes.
14. Increase or decrease the value by using the▼▲ arrow keys
15. Repeat 6 thru 14 above to program successive steps.

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Viewing a Program
1. Select the file number using the ▼▲ arrow keys when highlighted press the Mode
key.
2. Press the Mode key 3 more times to advance to the programming.
3. If multiple steps use the Mode key to advance through the program to the next step.
Editing a program
1. When a file is selected the parameters can be edited by the Mode button
2. When the parameter is selected, it will flash. Use the ▼▲ arrow keys to either
increase or decrease values.
3. Press the Mode key to migrate to the next parameter.
4. When the parameter is selected use the ▼▲ arrow keys to increase or decrease
the values.
C oosing Limiting Parameter Settings
Introduction
The ENDUR 250 Volt Power Supply is capable of operating at limiting Voltage, or limiting
Current or limiting Watts. We recommend operating the ENDUR 250 Volt Power Supply at
limiting voltage for most applications. See below for more details.
Voltage Limiting
For most electrophoresis methods resistance increases throughout the run. Limiting the
voltage provides the following advantages:
• Current and power decrease throughout the run, providing an improving margin of safety
over time.
• The same voltage setting can be used regardless of the number or thickness of gels being
electrophoresed.
Current Limiting
Discontinuous buffer systems and, to a lesser extent, continuous systems increase resistance
during the run. If you use the current limiting setting on the ENDUR 250 Volt Power Supply,
the voltage will increase as resistance increases to satisfy hm’s law (V=IR). If no voltage limit
is set and a local fault condition occurs, such as a poor connection, very high local resistance
may cause the voltage to increase to the maximum capacity of the power supply. This may
lead to local overheating and damage to the electrophoresis cell or create unsafe conditions.
When operating under constant current conditions, set a voltage limit on the power supply at or
slightly above the maximum expected voltage.

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Power Limiting
Watts is a function of voltage and current W=IV. If voltage is increased watts will also be
increased depending on the gel system, R should be a constant. Watts will be reflected by
the heat generated during a gel run. The power limiting function may be used when running
sequencing gels to remove the APS from the wells and to heat the gel to an optimal
temperature for DNA separations.
Troubles ooting
Review the information in the table below to troubleshoot operating problems.
Problem Cause Solution
The LCD screen
remains blank and
the fan does not
run
when the power is
turned on
AC power cord is not
connected
Check AC power cord connections at both
ends. Use the correct cords.
The fuse has blown Replace the fuse
peration stops
with alarm
The screen displays
“NO LOA ”
1.
Electrophoresis leads are
not connected to the power
supply.
2. The electrophoresis
unit(s), is not connected.
3. There is a broken circuit
in the electrophoresis cell
1. Check the connections to the power
supply and on your electrophoresis cell to
make sure the connection is intact; check
condition of wires in electrophoresis unit.
2. Close the circuit by reconnecting the
cables.
3. Press START/PAUSE to restart the run.
4. High resistance due to
tape left on a pre-cast gel
5. Incorrect buffer
concentration, or incorrect
buffer volumes in the
electrophoresis cell
4. Correct the condition by making sure the
tape is removed from the pre-cast gel.
5. Make sure buffers are prepared
correctly, and the recommended volume of
buffer is added to the electrophoresis unit.
6. High voltage application
is set to run on a very low
current
6. Change current setting.
peration stops
with alarm:
Circuit is interrupted
1. Verify that the running buffer is correct.
2. Verify the all cables are attached correctly

13
Display shows
“OVER VOLTAGE”
3. Turn the Power switch off and on again;
restart application.
4. If you cannot restart the instrument, turn
off the power, disconnect the power cord
from the outlet, and contact Technical
Service.
peration stops
with alarm:
Display shows
“LEAKAGE”
Ground leak detected
during run
1. Check the electrophoresis system for
improper grounding.
2. Restart the power supply by turning the
Power switch off and on.
peration stops
with alarm:
Display shows
“OVER TEMP”
Power supply is
overheating
1. Turn off power supply. Check for sufficient
airflow around the power supply fan. After
cooling down, restart the power supply.
2. If you cannot restart the instrument, turn
off the power, disconnect the power cord
from the outlet, and contact Technical
Service.

14
Operational Electrical Parameters
Power Considerations
Electrophoresis is the migration of a charged particle under the influence of an electrical field.
The power supply output parameters voltage, current, and power are related by the following
two equations:
Voltage (V) = Current (I) x Resistance (R); (V=IR)
Power (W) = Current (I) x Voltage (V); (W=IV)
Resistance
Resistance of the assembled electrophoresis cell is dependent on the conductivity of the gel
buffer, the thickness of the gel, and the number of gels being run. Although the resistance is
determined by the gel system, the resistance can vary over the course of an electrophoretic
separation. For instance, in the Tris-Glycine buffer system, the fast moving, highly conductive
chloride ions in the gel are gradually replaced by the slower moving, less conductive glycine
ions from the running buffer as the gel runs. As a result, the resistance of the gel increases as
the chloride/glycine front moves down the gel, and the current decreases.
Voltage
The velocity with which an ion moves in an electric field will vary in proportion to the field
strength (volts per unit distance). The higher the voltage the faster an ion will move.
Current
Current is a function of the number of ions passing a given cross-section of the circuit at a
given time. For a given gel/buffer system, at a given temperature, current will vary in proportion
to the field strength (voltage) and/or cross-sectional area (number and/or thickness of the gels).
Ions in solution and at a given voltage will move faster as the temperature increases,
increasing current.
Power
The power in Watts, or the rate of heat generated by the system, is directly proportional to
voltage and current (W=IV).

15
Common Errors found with Electrophoresis Power Supplies
No load
• The electrophoresis system is not connected to the power leads, check the power leads
• The electrophoresis system has a short, the Pt wire is broken or the banana connectors are
damaged
• Buffer concentration too low
• Buffer volume too low
• Short in power cord
• Current has dropped below acceptable rating (4 m )
Short circuit
• Load exceeds 500 m
• Blown fuse in the power supply
• Incorrect input voltage (check input voltage switch near power inlet)
Change in load
• Electrophoresis systems were added or removed during a run
• Buffer leaking in a connected system
• Excessive temperature increase
• Excessive buffer evaporation
• Loose connection in a connected system
• mperage set to low
Change in constant mode
• Voltage changes to amperage
1. mperage set to low. Ceiling hit and constant mode changed from voltage to amperage.
Increase amperage to 500 m .
• mperage changes to voltage
1. Voltage set to low. Ceiling hit and constant mode changed from amperage to voltage.
Increase voltage to 300 volts
The 300 volt system has automatic cross over, set voltage or amperage, and preset wattage. During the
electrophoresis process only one parameter is limiting at a time. The limiting parameter, together with
the conductivity in the electrophoresis system, and the values for the other parameters determine the
maximum output.

16
Repair and Maintenance of ENDURO 250 Volt Power
Supply
Introduction
The ENDUR 250 Volt Power Supply requires no periodic maintenance program with the
exception of an occasional dry wipe-down of the instrument.
Encountering Problems
1. Check the troubleshooting section.
2. Call Technical Service.
3. If the unit must be shipped back for repair, contact Labnet for a Return Authorization
Number and shipping instructions.
Replacing the Fuse
For additional fuses, contact Labnet International Technical Service.
To replace the fuse:
1. Turn off the main power switch at the rear of the ENDUR 250 Volt Power Supply and
detach the power cord from the rear of the ENDUR 250 Volt Power Supply.
2. pen the fuse compartment located inside the Power Entry Module by inserting a small flat
blade screwdriver into the slot below the N/ FF switch. Turn the screwdriver to gently
pry open the fuse compartment.
Note The fuse compartment will not open with the power cord in place.
3. Pull the fuse holder out of the compartment and inspect the fuse. If the fuse is burned or
there is a break in the fuse element, replace the fuse with an identical type of fuse (4A/250V)
as provided in the fuse holder (see figure below).
4. Place the fuse holder back into the compartment.
5. Snap the cover closed.

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Tec nical Service
Web Resources
Visit the Labnet’s website at www.labnetlink.com for:
• Complete technical service contact information.
• Access to Labnet’s nline Catalog, and information about accessories and related products.
• Additional product information and special offers.
Contact s For information or technical assistance contact your local Dealer or Labnet
representative.
Legal Address of Manufacturer
United States Europe
Labnet International Labnet International
31 Mayfield Ave. 31 Mayfield Ave.
Edison, NJ 08837 USA Edison, NJ 08837 USA
732-417-0700 732-417-0700
http://www.labnetlink.com http://www.labnetlink.com
Warranty
Labnet International, Inc. warrants that this product will be free from defects in material
and workmanship for a period of three (3) years from date of purchase. If a defect is present,
Labnet will, at its option, repair, replace, or refund the purchase price of this product at no
charge to you, provided it is returned during the warranty period. This warranty does not apply
if the product has been damaged by accident, abuse, misuse, or misapplication, or from
ordinary wear and tear.
For your protection, items being returned must be insured against possible damage or loss.
This warranty shall be limited to the replacement of defective products. IT IS EXPRESSLY
AGREED THAT THIS WARRANTY WILL BE IN LIEU F ALL WARRANTIES F FITNESS
AND IN LIEU F THE WARRANTY F MERCHANTABILITY.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.

18
Equipment disposal
This equipment is marked with the crossed out wheeled bin symbol to indicate that this
equipment must not be disposed of with unsorted waste.
Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by
handling it over to an authorized facility for separate collection and recycling. It's also your
responsibility to decontaminate the equipment in case of biological, chemical and or
radiological contamination, so as to protect from health hazards the persons involved in the
disposal and recycling of the equipment.
For more information about where you can drop off your waste of equipment, please contact
your local dealer from whom you originally purchased this equipment.
By doing so, you will help to conserve natural and environmental resources and you will
ensure that your equipment is recycled in a manner that protects human health.
Thank you.