Labomed TCM 400 FLR User manual

To ensure proper use of this instrument as well as to avoid injury while operating
instrument, understanding this manual completely before use is highly recommended.
User Manual
TCM 400FLR
Inverted Microscopy
R
7127000-795
________________________________________________________________________________________________________
Issue 1.1
Printed on Aug. 2018

TCM400Flr

CONTENTS
9 TROUBLESHOOTING 8
10 TECHNICAL SPECIFICATION 9
8 OBSERVING A SPECIMEN 7
7 CENTERING THE LIGHT BEAM 7
6 MAINTAINING OPERATING LIFE 6
5 INSTALLATION 4
4 SAFETY INSTRUCTIONS 3
3 UNPACKING 2
2 PRINCIPLE 1
1 INTRODUCTION 1

PACKAGING CONTENTS
1. FL attachment in 2 parts
2. 2 Filter cube
3. Power Supply
4. Power cord
5. UV protection shield
FluorescenceAttachment User Guide 2

SAFETY INSTRUCTIONS
Fluorescent attachment is manufactured according to CE safety norms and regulations.
Fluorescent attachment is intended for use only as prescribed in this manual.
Make sure the main switch on the power supply unit is set to OFF (O) before supplying power to the unit.
The power supply unit contains high voltage components. Do not attempt to disassemble the unit when turned ON
(I).
To avoid injury, do not remove the lamp housing while the lamp is lit. Also, do not turn the lamp ON while the lamp
housing is removed to avoid damaging the instrument.
Before opening lamp housing for replacement of the lamp, set the main switch to OFF (O) and unplug the lamp
housing's connecting cord plug from the output connector on the power supply unit. Wait at least 10 minutes or until
the lamp housing cools down before any changes are made.
Do not open the lamp housing while the system is turned ON, for any user contact with internal parts may cause
injury.
A used mercury burner should be disposed of in compliance with the ordinances or regulations of your national or
local government.
The manufacturer will not accept any liability for damage caused by any user following unsafe practice of the
instrument.
FluorescenceAttachment User Guide 3
Avoid using the lamp beyond its service life. The mercury lamp seals high pressure gasses. If the lamp is used beyond its service life,
stress may accumulate inside the lamp and in the worst but very rare case, the lamp may burst.

INSTALLATION
Remove all parts from the packing carefully.
1. Remove the plastic cover (1) from the microscope to make room for
the fluorescence attachment.
Bring the nosepiece to the maximum height by rotating the coarse
knob (2) anti- clockwise. Remove the illumination power cable before
removing the cover. Apply similar pressure by thumbs on the inner
sides of the panel (3 & 4) to push it out. Refer to figure 1 and 2.
2. Join the Optical assembly and illumination assembly to complete the
FL attachment. Guide the illumination assembly to the optical
assembly. Secure both parts by the allen screws (1) provided. Refer
Fig. 3.
3. Assemble attachment in the microscope as shown in Fig. 4 and
secure it with 3 Nos of Screws. Then install filter in the guide slot as
shown in Fig. 5.
FluorescenceAttachment User Guide 4
Fig. 4
Fig. 3
Fig. 5
Fig. 1
Fig. 2
2
34
1
1

AB
INSTALLATION
Open the illumination house to install the mercury lamp. Loosen the
thumbscrew indicated by the arrow and gently pull out lamp door from lamp
housing.
To avoid any damage to the mercury vapor lamp, a plastic rod (1) is fitted in
place of lamp during transit. Remove this rod by loosening the two screws
indicated by the two arrows A and B shown in Fig. 6.
Mount the mercury vapor lamp in place of plastic rod and tighten the screws
to secure the lamp. install as shown in Fig. 6.A.
Avoid touching the lamp in order to prevent leaving finger prints on the
glass. If there is any residue on the lamp's surface, gently wipe it off with
soft tissue paper.
Install the power cable coming from the illumination house to the power unit
indicated by arrow A in Fig. 7.
(1). Connect the power cord to theAC Inlet indicated by arrow B in Fig. 7.
FluorescenceAttachment User Guide 5
Fig. 7
Fig. 6
B A
Fig. 6A

INSTALLING THE FILTER B & G
Select the filter to be used on the fluorescence attachment.
Engage the slider to the filter house and gently slide it in.
It will stop at the Iindex position.
Ensure that the power supply unit has been connected to proper power supply voltage.
Set the main switch on the power supply unit to ( | ). After the lamp is ignited, at least 5 minutes required to
stabilizelight..
To avoid shortening the power supply unit's operating life, avoid turning power ON while the lamp is not mounted
Avoid turning power ON and OFF within short intervals for this may reduce working life of the power supply.
After the lamp is turned OFF, it should not be re-ignited before the mercury vapors cool and condense. Wait
approximately 10 minutes before turning power to the fluorescent attachment O N again.
MAINTAINING OPERATING LIFE
FluorescenceAttachment User Guide 6
Fig. 8

CENTERING THE LIGHT BEAM
First allow the mercury vapor lamp at least five
minutes to fully illuminate for optimal use (it is very
important to allow the system enough time to fully
ignite in order to achieve a fully illuminated centering
beam). Look into the eyeppiece aanddmanipulate
the ccollector focusing knobb to make the field asd
bright as regular asapossible, then, turn the knobb
clockwise to tighten as shown in Fig.#9/Part 1.
When the lamp is fully illuminated, a beam of light is
visible through the eyepieces in the field of view.
To center this beam of light in the field of view, two
centering knobs are located on the lamp housing as
indicated by the two arrows (A & B) in Fig. 9. By
adjusting these two knobs, the focused beam will
shift. Adjust the beam position to the center of the
field of view as shown in Fig. 9. A.
OBSERVING A SPECIMEN
FluorescenceAttachment User Guide 7
1
A
B
Fig. 9
Turn the power supply ON. Allow the lamp at least 5 minutes to
fully illuminate for optimal use.
The halogen illumination of the microscope should be kept off
while working with FLR.
Place the specimen slide on stage. Select the excitation light by
installing the filter block as follows :
For G-excitation - Install red sliding block to its outermost position.
For B-excitation - Push blue sliding block in to its innermost position.
For O light (non-excited light) - Slide out the filer to parking point.
Observe the specimen slide through the microscope's eyepieces.
When no fluorescent light is required, turn the attachment's power
supply OFF and set the sliding filter block to its middle / neutral
position. To view the specimen in normal condition under the
microscope's light, turn the microscope ON.
Fig. 9A.

TROUBLESHOOTING
FluorescenceAttachment User Guide 8
Problem Cause Remedy
Optical System
1) The mercury lamp lights up, Filter slider is not engaged properly Adjust filter to required setting.
but field of view remains dark in light path.
2) Image is unclear, blurred or a) Objectives and/or filters are dirty. Clean them with tissue/lens paper.
has poor contrast. b) Field iris diaphragm is not Adjust field iris diaphragm so
fully opened. that the image circumscribes
the field of view.
3) Field of view is vignetted or a) Objective is not engaged properly Be sure when rotating nosepiece
it is not evenly illuminated. in light path. that objective clicks into position.
b) Filter slider is not engaged properly Engage required filter
in light path. properly in light path.
c) Mercury lamp is not centered Center mercury lamp correctly.
properly. This process is shown on Page 7
d) Beam focus deviates from correct Adjust the condenser knob.
position. This process is shown on Page 7
4) Mercury lamp is not illuminating Some mercury lamps may not ignite Press the Trigger button provided
after switching power supply ON. the first time the power supply is on the front panel of power supply
turned ON. unit and wait for lamp to ignite.
Electrical System
1) Lamp housing / Main switch a) Power cord is not connected Make sure power cord is firmly
indicator does not light up. properly. in place.
b) The fuse is blown. Replace with fuse of same type
and rating.
2) Main switch indicator lights up, a) Connector cable is not engaged Verify connection is secure.
yet mercury lamp remains OFF. properly.
b) Mercury lamp is not mounted. Install mercury lamp as
indicated on Page 5
3) Light demonstrates partial a) Sufficient time has not been Allow at least 5 minutes after
flickering. allowed for the lamp to fully turning power supply ON for the
illuminate. lamp to fully illuminate. If flickering
persist press Trigger button.
b) Lamp's service life has expired. Replace the mercury lamp

TECHNICAL SPECIFICATIONS
FILTER SPECIFICATIONS
Type : Transmitted Fluorescent illumination based on switching of dichroic mirror
cubes
Observation modes : B-excitation and G-excitation
Lamp type : HBO 50 W, high pressure Mercury vapor lamp
Supply Voltage : 110V AC, 60 Hz / 220V AC, 50Hz
Operating environment : Indoor use 0 0
Ambient temperature 5 C to 40 C 0
Maximum relative humidity 80% at 40 C
B-excitation
Excitation filter : Transmission 90% at wavelength 420 to 480 nm.
Transmission 0% at wavelength 485 nm onwards.
Dichroic mirror : Transmission 93% at wavelength 540 nm onwards.
Transmission 0% at wavelength 400 to 530 nm.
Barrier filter : Transmission 95% at wavelength 535 nm onwards
Transmission 0% at wavelength 400 to 500 nm.
Recommended Fluorescent Dye : Acridine Orange, both DNA & RNA
G-excitation
Excitation filter : Transmission 85% at wavelength 490 to 525 nm.
Transmission 0% at wavelength 530 nm onwards.
Dichroic mirror : Transmission 90% at wavelength 600 nm onwards.
Transmission 0% at wavelength 400 to 595 nm.
Barrier filter : Transmission 92% at wavelength 615 nm onwards
Transmission 0% at wavelength 400 to 575 nm.
TM
Recommended Fluorescent Dye : Acridine Red FM 5-95
FluorescenceAttachment User Guide 9

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Our policy is one of continuous development. Labo America, Inc., reserves the right to change design and specifications without prior notice.
LABOMED and TCM400 FLR are registered trademarks of Labo America, Inc.
With a policy of continuous development, Labo America, Inc. reserves the right to change design and specifications without prior notice.
© 2011 Labo America, Inc. | 7127000-795 08-2018
Labo America Inc.
920 Auburn Court
Fremont
CA 94538
U.S.A.
Telephone: [phone]
Fax: [phone]
[e-mail] A28240
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