Labomed CxL Dark Field Kit User manual

CxL Dark Field Kit
User Manual
To ensure proper use of this instrument as well as to avoid injury while operating instrument,
understanding this manual completely before use is highly recommended. www.laboamerica.com
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CxL Dark Field Attachment
PACKING LIST
INTRODUCTION
Darkfield Attachment User Guide 1
Darkfield microscopy is a specialized illumination technique that capitalizes on oblique illumination to enhance contrast in specimen that
are not imaged under normal brightfield illumination conditions.After the direct light has been blocked by an opaque stop in the substage
condenser, light passing through the specimen from oblique angles at all azimuths is diffracted, refracted and reflected into the
microscope objective to form a bright image of the specimen superimposed onto a dark background.
Ideal specimen for darkfield illumination include minute living aquatic organism, diatoms, small insects, unstained bacteria, yeast and
protozoa. Non-biological specimen include mineral and chemical crystals, colloidal particles, dust-count specimens, and thin sections
of polymers and ceramics containing small inclusions.
12 3 4
1. Darkfield condenser (3126050)
2. 100X (SL, Oil) Achro objective (Iris) (9131105)
3. Immersion oil bottle (PK-035)
4. Darkfield condenser NA-1.25, oil, optional (3124044)
Pleas make sure all contents of the Phase Contrast Kit are intact. If any physical damage is visible, please
contact your local distributor.

INSTALLATION
Darkfield Attachment User Guide 2
Fig. 1
Fig. 2
Fig. 4
Fig. 5
Fig. 3
1. Remove brightfield condenser from substage assembly and gently push in
darkfield condenser in its place as shown in Fig.1
2. Secure the darkfield condenser by tightening the thumbscrew provided as
shown in Fig. 2
3. Remove the 100X objective from nosepiece adapter and attach 100X (SL, Oil)
achro objective (Iris) as shown in Fig.3
4. Bring the darkfield condenser to its top most position and pour a drop of
immersion oil (PK-035) as shown in Fig. 4
5. Firmly place the specimen slide over the darkfield condenser in a way so that
it touches the drop of the oil as shown in Fig. 5.

CALIBRATING THE INSTRUMENT
CARE & MAINTENANCE
Fig. 6
Fig. 7
Fig. 8
Note : An optional darkfield condenser can also be used in place of brightfield condenser for darkfield observation. In that case you will
have to replace normal brightfield condenser with darkfield condenser NA 1.25, oil (3124044) but result will not be so good as
with 3126050 darkfield condenser.
6.
slide, a bright ring of light will appear in field of view. Bring this ring of light in the
center of the field by manipulating two thumbscrews provided on the condenser
bracket as shown in Fig. 6
7. Again pour a drop of immersion oil on slide as shown in Fig. 7
8. Engage 100X (Oil Immersion) darkfield objective in field of view. Keep open
the Iris provided in the 100X objective by turning knurled ring clockwise.
Contrast in the darkfield can be increased or decreased by adjusting the
opening of Iris in 100X objective.
Engage 4X objective in field. while viewing through the eyepiece focus specimen
?
wiping them with lens tissue dampened with Acetone or Methanol.
?It is better to avoid leaving finger prints on the surface of darkfield condenser.
?When not in use for long time, it is recommended to store the darkfield attachment in its original packaging.
Loose particles can be removed from the surface of the darkfield condenser, diaphragm, or darkfield annulus by gently
© 2009 Labo America, Inc. | 9134050-990A 12-2009
LABOMED and CxL are registered trademarks of Labo America, Inc.
With a policy of continuous development, Labo America, Inc. reserves the right to change design and specifications without prior notice.
Labo America Inc.
Our policy is one of continuous development. Labo America, Inc., reserves the right to change design and specifications without prior notice.
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