Labomed Lx 400 User manual

To ensure proper use of this instrument as well as to avoid injury while operating
instrument, understanding this manual completely before use is highly recommended.
User Manual
Lx 400
Research Microscopy
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CONTENTS
9 ASSEMBLY 13-14
12 TROUBLESHOOTING GUIDE 21-22
13 SPECIFICATIONS 32
11 DETAILED OBSERVATION PROCEDURE 16-20
10 SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE 15
8 INITIAL SETUP 12
7 OPTIONAL ACCESSORIES 9-11
6 STANDARD COMPONENTS 8
5 UNPACKING YOUR MICROSCOPE 7
4 Lx 400 TRINOCULAR 6
3 Lx 400 BINOCULAR 5
2 SAFETY INFORMATION 2-4
1 INTRODUCTION 1

The Lx 400 is a research microscope reflecting a modern design as well as the latest in optical and mechanical
advancements. Designed for professionals as well as clinicians, this microscope offers many features and functions for a
diverse set of applications. Here are a few points highlighting the benefits of the Lx400:
- Extra clarity and contrast is provided through a 360° rotatable Binocular body inclined at 30° with IPD adjustments.
- The pressure die cast stand consists of ball bearing, frictionless sideways focusing to avoid any loss in motion.
- The sturdy new stylish design provides a high degree of comfort as well as stability.
- The high powered objectives are spring loaded to prevent accidental damage to specimen slides.
- The reverse angle quadruple nosepiece has a comfortable ribbed grip for easy rotation that also safeguards the turret
system against any damage. All positions are par-centered and par-focalised ensuring the highest level of accuracy.
- The ball bearing mechanical stage allows smooth travel over a 78 x 54mm area with spring loaded stage clamps for retaining
the specimen at the exact position desired. A0.1mm vernier scale provides accurate location of specimen area.
- High power illumination is delivered through our well crafted Universal Power Supply and operates with any 100V- 240V AC
input. The LED configuration is operational with an in-built rechargeable battery P/N 9135000-902, which when plugged
in, draws power from a Universal Power Supply operating at 100V-240V Ac input. This ensures continuous operation even
under fluctuating voltages.
- Our Halogen bulb (6V-20W) has an average life span of up to 2,000 hours. Our LED has an average life span of up to
100,000 hours.
- The Lx 400 comes equipped with a removable N.A. 1.25 Abbe condenser with aspheric lens for brighter illumination levels,
and an iris diaphragm for better resolution and contrast control.
INTRODUCTION
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1. After the microscope has been used for observation of a specimen
containing bacteria, clean all parts coming in contact with the specimen
to prevent infection.
¡Be sure to remove the specimen before moving this product.
¡In case the specimen is damaged by erroneous operation, it is important
to clean all surfaces that may have come in contact with the specimen.
2 To avoid potential electrical hazards when replacing either halogen bulb
or LED, turn the microscopes main switch to the OFF position and
disconnect power cord from wall outlet in advance. Whenever you
replace your microscope bulb, allow lamp socket and bulb to cool before
touching (Fig. 1)
Applicable bulb/LED replacement: 6V20W Halogen bulb P/N CX-013; 6V30W Halogen bulb P/N EL-455
or LED P/N 9135000-901
3. Install microscope on a sturdy, level table or bench and avoid any restriction of air vents in the base of the unit.
Do not place microscope on a flexible surface, as this could result in blocking the air vents and cause overheating.
4. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety
performance cannot be warranted.
5. When installing the microscope, route the power cord away from the microscope frame. Should the power cord
come in contact with the microscopes base, the power cord could melt due to overexposure to heat.
6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If
the unit is not grounded, LABOMED can not warrant electrical safety.
7. Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and
damage to the microscope.
8. After operation of microscope, be sure to disconnect power cord from connector socket of the microscope or from
the wall power outlet.
Fig. 1
Safety Symbols
Symbol Explanation
This surface has a tendency to heat up and should not be touched unless
system has completely cooled down.
Before use, carefully read the instruction manual. Improper use could result in
injury to the user and/or damage to the equipment.
Main switch is ON.
Warning against risk of electric shock.
Main switch is OFF.
The following symbols are found on the microscope. For optimal use, it is recommended that users understand
these symbols and always use the equipment as prescribed.
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SAFETY INFORMATION
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Lx 400

1. A microscope is a precision instrument with delicate glass components.
Please handle with care.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature,humidity, dust and vibrations. (For the operating
conditions, see chapter 14, "SPECIFICATIONS")
3. The microscope is ventilated by natural convection. Be sure to leave
enough space (10 cm or more) around body when installing the unit.
4. Arm handle is provided for carrying the microscope.
To prevent damage, do not hold the microscope by the stage or
observation tube.
Be sure to remove the specimen from the stage clip while
transporting unit to avoid damage to the specimen slide.
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Getting Started
1. Clean all glass components by wiping gently with cleaning cloth provided. To remove fingerprints or oil smudges,
wipe with cleaning cloth slightly moistened with a mixture of petroleum (85%) and isopropanol (15%).
Since solvents such as petroleum and isopropanol are highly flammable, they must be handled carefully.
Be sure to keep these chemicals away from open flames or potential sources of electrical sparks - for
example, electrical equipment that is being switched “ON” or “OFF”. Also remember to always use these
chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To
clean non-glass components, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope as this could result in malfunction or mitigated performance.
4. When not using the microscope, ensure that the frame is fully cooled
before storing the unit in a dry locker or covering with a dust cover
(provided).
5. To clean the condenser/phase turret, fully loosen the securing thumb
screw(1) and remove the condenser then, wipe the front lens of the
condenser with optical cleaning solution (mixture suggested above)
and lens tissue.
The condenser can be re-attached by replacing the condenser in its
seat, tightening securing thumb screw, and raising condenser bracket
to desired position.
6. Be sure to observe your local rules/regulation for product disposal.
Fig. 2
Fig. 3
Maintenance and Storage
3
Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may not be warranted. In
addition, the equipment may also suffer damage. Always use the equipments as outlined in this instruction manual.
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3Care & Maintenance
Your microscope has been engineered for a long and safe operational life with the least amount of maintenance
required. In general, routine maintenance is limited to keeping the microscopes working parts lubricated and optics
clean.Always cover the microscope with the provided dust cover when not in use.
Optical Cleaning:
1. The objectives have been adjusted for a tight fit to prevent any damage during transportation. To remove an
objective, rotate it counterclockwise while gripping it with a rubber sheet, etc. to avoid any slippage.
2. To clean the lens surfaces, remove dust using a soft brush or compressed air (cans available at your local
electronics store). For removing finger marks or grease, soft cleaning cloth or lens tissue lightly moistened with
cleaning solution (85% petroleum ether and 15% isopropanol) should be used. For cleaning the objective optics,
use Methanol. Observe sufficient caution in handling Methanol. Place the objectives and/or eyepieces on a dust-
free surface (e.g. aluminum foil).All other optical components to be cleaned should be as accessible as possible.
3.. Blow all loose dust particles away with compressed air or mini dust blower.
4. Remove all water-soluble dirt with distilled water. If this is unsuccessful repeat using a solution of diluted hand
soap liquid. Remove any remaining residue with a dry cotton swab.
5. To remove oil, use a solution of diluted hand-soap liquid initially. If this does not produce a satisfactory result,
repeat the cleaning using a solvent (Optical Cleaning Solution 85% petroleum ether and 15% isopropanol).
6. Grease must always be removed using a solvent.
7. Cleaning is achieved by using a spiral motion from the center to the rim. Never wipe using zig-zag movements as
this will only spread the dirt. With larger optical surfaces (e.g. tube lenses) the spiral motion starts initially at the rim
before moving to the middle and is then followed by a center to rim cleaning motion. Normally several spiral wipes
are recommended.
We recommend pure, volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above.
Wipe using a spiral movement. Do not use a zig-zag motion!
2. Cleaning of painted surfaces :
Avoid the use of any organic solvent ( e.g. thinner, xylene, ether, alcohol etc.) for cleaning of painted surfaces of
the instrument. Painted surfaces can be cleaned with a very lightly moistened micro fiber cloth. Loose dust and
other dirt particles can be removed using a soft bristle brush used exclusively for this purpose.
zig-zag motion (X) spiral motion ( )
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Lx 400

5
Lx 400 Binocular
3Lx 400
X-Y movement control knob
Binocular viewing tube, 30° inclined
Observation tube
clamping screw
Coarse and
fine focus knob
Mechanical
stage
On/Off intensity regulator
Specimen holder
Revolving nosepiece
Objectives
Eyepieces
Abbe condenser
Filter holder
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Lx 400 Trinocular
4
X-Y movement control knob
On/Off intensity regulator
Eyepieces
Revolving nosepiece
Objectives
Specimen holder
Coarse and
fine focus knob
Mechanical
stage
Observation tube
clamping screw
Trinocular viewing tube
Trinocular Port
Abbe condenser
Filter holder
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UNPACKING YOUR MICROSCOPE
5Lx 400
Microscope Arm
Observation head
Eyepieces
Power Cord

8
¡After removing your microscope from its packaging, make sure that all of the following contents are present.
“Please note that the contents of your microscope may vary as the optional configuration, contrasting method or
viewing body opted for may not be of the standard configuration highlighted here”
STANDARD COMPONENTS
6Lx 400
Daylight (blue) filter
Power Cord
Allen Wrench 3mm
Paired Eyepieces
6V 20W Halogen bulb
Monocular/ Binocular/ Trinocular observation tube
Allen screw
Microscope Arm
Stage
Objectives
Eyepieces
(Fixed at 10x)
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System Diagram of Optional Accessories
Installation and Operation of Optional Accessories
1.Mount the Video adapter 1/2” (part # 3143300-912) on Trinocular
observation head.
2. Mount iVu Camera Module System on video adapter.
1iVu Camera Module System
Fig. 4
OPTIONAL ACCESSORIES
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Video adapter
Binocular head
Abbe Condenser 1.25 Abbe Condenser 0.6 Polarizing kit Phase turret Blue filter Green filter
RP 4x RP 10x RP 20x RP 40x (SL) RP 50x (SL, Oil) RP 100x (SL, Oil) RP PH 10x RP PH 20x RP PH 40x (SL) RP PH 100x (SL, Oil)
RP FL 4x RP FL 10x RP FL 20x RP FL 40x (SL) RP FL 50x (SL, Oil) RP FL 100x (SL, Oil) RP 100x,(Oil, Iris)
Trinocular head WF 10x WF 16x WF 20x
DSC adapter iVu 1500 iVu 3000
Halogen Bulb LED Mount Rechargeable Battery
iVu 5000 iVu 7000
iVu 5000
iVu 3000
iVu 7000
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1. Remove the condenser from its position by loosening the thumb screw
(1) on right side of the condenser bracket as shown in figure 6.
2. Place the phase annulus turret in position from where the condenser
has been removed.
3. Secure it with thumb screw (1). The phase turret can be adjusted at any
desired phase annulus (10x, 20x, 40x & 100x) by turning the
thumbwheel (2) to the desired position. There is also an ‘0’ setting on
the thumbwheel for bright field application
Note: Refer to the user manual provided with the Phase Contrast
kit for centering and alignment.
10X eyepieces are provided. To replace:
1. Pull out the 10x eyepieces out from the observation heads ocular tube.
2. Insert desired eyepieces in empty ocular tube.
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Phase Turret
Optional Eyepieces
Fig. 5
Fig. 6
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Lx 400
Procedure for Koehler Installation kit (figure 7-11)
1 Lay the microscope on a clean surface toward right so that the
microscope base is exposed.
2. Using a screw driver, unscrew four screws (1 to 4) from (figure 7) to
open the base of the microscope.
3. Remove two screws (A) and (B) from (figure 8) to open the illumination
mount and replace it with Koehler mount.
4Installing the Koehler Illumination Kit
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Fig. 8
AB
Fig. 7
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Lx 400
Fig. 9
4. Open the illumination assembly as in (figure 9), by rotating the T Ring.
Replace the Lens B with frosted lens provided with the Koehler kit.
Secure the lense assembly back.
5. Close the base cover with screws.
6. Replace the blue filter with D Lens. Refer figure 10.
7. Install the new Blue filter on the Koehler mount, figure 11.
Caution: The flat surface of the lenses will remain downwards for
Lens A and Lens B.
Fig. 10
Fig. 11
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Lens A
Frosted
Lens A
Lens Spacer
Lens B
T Ring
Rotate anti clockwise
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INITIAL SETUP
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Objectives
Observation Head
Eyepieces
Objectives are factory set. Objectives are par-centered and par-
focalised during assembly.
All objectives have been secured for a tight fit to prevent them from
coming loose during transit. To remove an objective, rotate it
counterclockwise while holding it with a rubber grip to avoid any
slippage.
Install the observation head using the following procedure:
1. Using allen wrench 3mm (provided), loosen the Head Locking
Screw (1) and remove the dust cover cap provided in dovetail
cavity as well as on observation head dovetail.
2. Mount the Observation Head by engaging the dovetail provided
at the bottom of the head into the dovetail cavity provided in the
microscopes arm.
3. Tighten the Head Locking Screw (1) after positioning the
Observation Head as desired. See figure 13.
Fig. 12
Fig. 13
Fig.14
1
Insert the eyepieces into the ocular tube of Observation Head using
following procedure:
1. Remove the protective caps from the observation tube.
2. Insert 10x eyepieces into the ocular sleeve and tighten
the clamping screw (1) using allen wrench 1.2mm. See
figure 14.
Lx 400
Lx 400
Lx 400

1Installing or Replacing the Lamp Bulb
Each standard set can be assembled by simply attaching the bulb, filter and power cord.
13
ASSEMBLY
9Lx 400
Before attaching the lamp bulb, remove the parts that may drop such as the
filter and specimen from the microscope frame, and place the microscope on
its back so that the bottom plate is exposed.
1. Pull the lock knob (1) on the bottom to open lamp housing door (fig.15).
2. Hold the halogen bulb (2) without taking it out of the polyethylene bag so
as not to taint the bulb with fingerprints and push the bulb into the pin holes
on the socket (3).After attaching, remove the polyethylene bag.
3. With the lock knob pulled out, close the lamp housing door, then push the
lock knob back to lock the cover.
Always use the designated bulb. Using a bulb other than those
specified by LABOMED may lead to a fire hazard. Fingerprints or
stains on the lamp bulb reduce its life. If contamination occurs, wipe
bulb surface with a cloth slightly moistened with alcohol.
Caution: For Bulb Replacement During Use or Right After Use
The bulb, lamp socket and areas near these will be extremely hot during and right after use. Set the main
switch to" O" (OFF), disconnect the power cord from the wall outlet, and allow the bulb and lamp socket to
cool before replacing the bulb with a new bulb of the designated type. Cooling time may vary to users
discretion.
Procedure for LED replacement (figure 16):
1. Lay the microscope on a clean surface toward right so that the
microscope base is exposed.
2. Using a screw driver, unscrew four screws (1) to open the base of the
microscope.
3. Remove three screws (2) provided on the lamp housing (use screw
driver) to open the lamp housing.
4. Open the LED mount by opening the two scres (3).
5. Replace existing LED mount with live LED mount.
6. Reverse the steps 4 to 1, to complete the process.
Applicable bulb/LED replacement: 6V20W Halogen bulb P/N CX-013; 6V30W Halogen bulb P/N EL-455
or LED P/N 9135000-901
Fig. 16
Fig. 15
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3
1
Fig. 17
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Fig. 18
Lx 400
Before replacing the fuse, remove the parts that may drop such as the filter
and specimen from the microscope frame. Turn around the microscope to
its back so that theAC inlet is visible.
1. Use a flat head screw driver to open the fuse holder (1).
2. The fuse tray will come out with (2) live fuse and (3) spare fuse. Do not
pull out the fuse tray with force as it is locked and will not be out
completely.
3. Replace the primary fuse (2) with the spare fuse.
4. Engage the fuse tray back in.
Always use the designated Fuse. Using a fuse other than those
specified by LABOMED may lead to a fire hazard.
Caution: For Fuse replacement
Set the main switch to" O" (OFF), disconnect the power cord from
the wall outlet.
3Installing or Replacing the Fuse
Fig. 19
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This filter modifies the color of observation light into a natural (daylight
color).
• Fit the filter (1) into the bottom of the condenser (2) until it clicks into
(Figure 18).
2Mounting the Daylight (Blue) Filter
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Flip the main switch to “ON”
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen in focus.
Adjust the aperture iris diaphragm.
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the observation tube and
Adjust the interpupillary distance.
Adjust the dioptric setting.
Engage the required filters.
Adjust the brightness.
Observe Specimen.
Main switchFuse Holder
SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE
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eyepieces
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1. Flip ” ” as shown in Figure 19.
2. Rotating the light intensity adjustment knob (Fig. 20) in the direction
of the arrow increases brightness and rotating knob in the opposite
direction decreases brightness. The intensity bar next the knob
indicates the direction of intensity level.
the main switch to I(ON)
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Turning the Lamp ON
Placing specimen on the stage
Fig. 21
Fig. 22
Fig. 20
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DETAILED OBSERVATION PROCEDURE
11 Lx 400
1. Rotate the coarse adjustment knob (2) in anticlockwise direction to fully
lower the stage.
2. Open the bow-shaped lever (3) outward by pulling on lever handle (1),
place the specimen by sliding the specimen glass plate(s) on the stage
from the front toward the slide seat at the rear.
3. After positioning your specimen slides,(2 max) return the bow-shaped
lever (3) gently by slowly releasing control knob (1).
4. Rotating the upper co-axial knob controlling the Y-axis movement (4)
moves the specimen in the vertical direction. Rotating the lower knob
(5) moves the specimen in the X- axis or horizontal direction.
Do not adjust the specimen holder directly by hand, for this will
damage the rotary mechanisms.
When the specimen holder reaches stop position, the rotation
force of the X/Y knobs become stiff. Stop rotating the at this point.
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“Vernier Scales”
These scales allow for easy identification of the specimen’s position
(coordinates), making it easy to return to a particular region of interest
after scanning the slide. (Figure 23.)
1. The horizontal coordinate can be read at position (1) on the specimen
holder.
2. The vertical coordinate can be read at the index line (2).
Focusing Procedure (Figure 18)
1. Rotate the coarse adjustment knob (1) clockwise so that the objective
(3) is as close as possible to the specimen (We recommend starting with
10X).
2. While observing the specimen through the eyepieces, slowly rotate the
coarse adjustment knob (1) counterclockwise to lower the stage.
3. When coarse focusing of the specimen is obtained (an image is
observed), rotate the fine adjustment knob (2) for fine detail focusing.
Working Distance (WD)
The WD refers to the distance between each objective and the specimen,
when acute focus of the specimen is obtained.
3Adjusting the Focus
Objective
Magnification 4X 10X 40X 100X
WD (mm) 30.5 4.82 0.55 0.11
Cover glass
This is the glass plate placed on the specimen. For optimum optical
performance, the cover glass thickness, which is the distance from its
surface to the specimen surface, should be 0.17 mm.
Slide glass
This glass plate should ideally have a length of 76 mm, width of 26 mm
±1 mm and thickness between 0.9 and 1.4 mm.
Cover glass
Slide glass
Fig. 23
Fig. 24
Fig. 25
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2
1
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3
WD