LABSTAC MIX37-25A User manual

OPERATION MANUAL
Thermo Shaker Incubator
SHA74-1500
OPERATION MANUAL
Vortex Mixer
MIX37-25A & MIX37-25
OPERATION MANUAL
Thermo Shaker Incubator
SHA71-1600

PREFACE
Thanks for choosing thermo shaker incubator. This operation manual describes function and
operation of the instrument. In order to use the instrument properly, please read this manual
carefully before operating the Instrument.
Opening Check
Please check the instrument and appendix with the packing list when you first open the
packing case. If anything does not match with the packing list, please contact with the vendor
or the producer.
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Thermo Shaker Incubator SHA71-1600

S fety W rnings nd Guidelines
1. Important Operation Information of The ecurity
Users should have an entire conception of how to use the instrument properly before
operating it. Please read this operation manual carefully before using the instrument.
2. ecurity:
To operation, maintenance and repair the instrument, please comply with the basic
guidelines and the remarked warnings below. Otherwise, the instrument will suffer effect
on the scheduled working life and also on the protection provided.
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It is forbidden operating before read the operation manual. Read the guidelines
and directions below and carry out the countermeasure according to them.
This product is a normal and an indoor using instrument.
Before operation, read the manual carefully. These units are
designed for using in the laboratory environments by who’re
knowledgeable in safe laboratory practices.
The operator should not open or repair the instrument by himself. Otherwise,
the instrument will lose the qualification of repair guarantee or cause
accidents. The company will repair the instrument based on warranty
description.
A.C. power’s grounding should be reliable to safeguard against an electric
shock. The 3-pin plug supplied with thermo-shaker’s power cable is a safety
device that should be matched with a suitable grounded socket.

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Thermo Shaker Incubator SHA71-1600
The temperature of metal block will be very high during the normal operation.
There will be scald or boiling of the liquid. It is strictly prohibited any part of the
body touching the instrument from scald.
Close the test tube lid before put the tube into the block. Liquids may spill out
in the block or onto the device if the tube lid is opened, which will damage the
block or the device.
Make sure the voltage is complying with the voltage required. Make sure the rated
electrical outlet load no lower than the demand. Power line should be replaced with
the same type if it any damage. Make sure there is nothing on the power line. Hold
the jack when pull out the power line. Do not pull the power line. Do not put the
power line in ambulatory place.
The instrument should be put in the place where of low temperature, little dust, no
water, no sunshine or hard light, and of good aeration, no corrosively gas or
strong disturbing magnetic field, and far away from central heating, camp stove
and other hot resource. Do not put the instrument in wet and dusty place. The
vent on the instrument is designed for aeration. Do not wall up or cover the vent.
The distance between each device should be more than 100cm when there is
more than one instrument.
Main switch is on the rear of the device. Turn to “I” to power on the
device, and Turn to “O” to power off the device.
Power off when operation finished. If long period do not use the instrument,
pull off the connector plug, cover a cloth on the instrument to prevent from
dust.
Pull the connector plug from the jack at once in the following case, and contact
the vendor.
• There is some liquid flowing into the instrument;
• Drenched or fire burned;
• Abnormal operation: such as abnormal sound or smell;
• Instrument dropping or outer shell damaged;
• The function has obviously changed.

3. The maintenance of Instrument
The well in the block should be cleaned by the cloth stained with alcohol to assure good heat
translation between the block and the test tube and no pollution. If there are smutches on the
instrument, clean them with cloth.
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Power off when cleaning the instrument.
Do not drop the clean fluid in the well when cleaning.
Corrosive clean fluid is strongly prohibited.

Index
1. Introduction............................................................................................07
2. pecifications.........................................................................................08
3. Preparations...........................................................................................10
4. Operation Guide.....................................................................................13
5. Failure Analysis And Troubleshooting....................................................20
Annex A Wiring Diagram of HA71-1600...................................................22
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Thermo Shaker Incubator SHA71-1600

01. Introduction
The Thermo haker Incubator is an ideal instrument for intensive mixing of samples in the
regulated temperature conditions.
haking and thermo modes (heating or cooling) can be used both simultaneously and
independently as well as time setting. It is a shaker while it is a thermostat. The main body of
the mixing block can be used with different kinds of blocks. The instrument is applicable for
DNA analysis, extraction of lipids and other cell components, DNA library creation, PCR
amplification, pre-denaturation in electrophoresis, serum solidification, etc.
Features:
1. Optimized 2D mixing control, over 3mm mixing orbit.
2. LCD display. Easy to set and use.
3. Accurately control and display time, temperature and speed.
4. Overheating protection device.
5. Temperature can be calibrated to meet user's needs.
6. Low noise and stable working.
7. Conforms to CE safety standard
8. Customized blocks are available to satisfy experimental requirement.
9. Can be used for micro test tubes, PCR plates, deep-well plates and MTPs
10. Gentle, reliable mixing with long-life brushless motor
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02. Speci*cation
1. The normal operating condition
Ambient temperature: 5°C ~30°C
The relative humidity: <70%
Power supply: 100-230V
～1.5A 50-60Hz
2. The basic parameters and specifications
Model P r meter HA74-1500
Mixing r te 200~1500 rpm
Orbit 2mm
Temper ture setting r nge 0C~100C
Temper ture control r nge
0C~100C@ Room temp. <20 °C
4C~100C@Room temp.<25C
10C~100C@ Room temp.<30C
Timing r nge 1min ~ 99h59min
Accur cy of the temper ture <0.5C
Temper ture st bility @100C:± 0.5C
He ting time
From 25 C TO 100C ^15min (The ambient
temperature is 20°C~30C)
Cooling time
^20min (ET^20C:20C~0C)
^20min (ET^25C:RT~4C)
<20min (ET^30C:RT~10C)
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Thermo Shaker Incubator SHA71-1600

St nd rd Block
A-BLOCK: 96x0.2 ml F-BLOCK:24x <012mm
tube
B-BLOCK: 54 x 0.5ml G-BLOCK:12x15ml
Falcon
C-BLOCK: 35 x 1.5ml H-BLOCK: 6x50ml Falcon
D-BLOCK: 35 x 2.0ml E-BLOCK: 20 x 1.5ml +
15 x 0.5ml
Fuse 250V 3.0A 05x20
Dimension (mm) 300(D)x225(W)x195(H)
Net weight (kg) 8.5
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03. Preparation
This chapter mainly describes the instrument’s mechanical structure, the keyboard and
functions of each key, as well as preparations before power on. Please learn this chapter well
before the Thermo haker to be operated the first time.
1. tructure Description
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Thermo Shaker Incubator SHA71-1600
St nd rd Block
Keybo rd
Power
Connector
Fuse
Power
Switch

2. Keyboard and Display panel
Display Panel:
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Current Speed
Current Temperature Remnant Time
S1 100.0 1200 25:05
S1 100.0 1200 30:00
Segment in service
Setting Segment Setting Speed
Setting Temperature Setting Time

3. Key Functions
Seg.

Key for selecting procedure segment. Five segments can be selected
( 1, 2, 3, 4, 5).
Temp.

Key for setting temperature. Press or to set the target temperature.
Press or continuously can quickly set temperature conveniently with
x10 speed. Continuously press till it displays “OFF” on the LCD, shut
the thermo function.
Speed

Key for setting speed. Press or to set the target speed. Press or
continuously can quickly set speed conveniently with x10 speed.
Continuously press till it displays “OFF” on the LCD to shut off the
shaking function. peed setting unit is 1rpm.
Time

Key for setting timing. Press or to set target timing value. Press or
continuously can quickly set timing conveniently with x10 speed.
Continuously press till it displays “OFF” on the LCD to shut off the
timing function.
Prog.
Key for programming. Press “PROG” to select segment. Default
starting segment is 1. The instrument can implement 4 programs as
1- 2，1- 2- 3，1- 2- 3- 4，1- 2- 3- 4- 5.
Short Mix
Press to shake, release to stop key. The device shakes at the speed
of current selected segment.
St rt/Stop
Key for start or stop. Press tart/ top key to start or stop the
program. To stop the program in operation need to keep pressing the
start/stop key for around 2 seconds.
He ting
The indicator light is flickered when heating or cooling. The indicator
stays light on when keeps the reaching temperature.
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Thermo Shaker Incubator SHA71-1600

04. Operation Guide
1. Setting Single Temper ture, Speed nd Timing
a) The instrument enters into the initial program as the chart
on the right with a beep when power on.
b) After 6 seconds, the LCD display program, e.g. as the right chart.
“ 1” is the segment run in last operation. “30.0” indicates current
temperature of the block. “37.0” is setting temperature, “1000”
is setting speed, “10:00” is setting time in last operation.
Temperature unit is °C, speed unit is rpm, and time unit
is hour:minute.
c) Press or of Temp, the temperature setting value will
increase or decrease from decimal digit, unit digit, tens digit
to hundreds digit.
Press or of peed or Time to set shaking speed or time.
Continuously press or to increase or decrease the value
from unit to tens digit, to hundreds digit quickly.
Instrument confirms and autosave the setting value.
After finishing setting program 1,
press “ tart/ top” to run 1.
When program finished, it will alarm a beep five times.
haking will be stopped while temperature kept
at the setting value.
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System-Testing
......
S1 30.0 0 00:00
S1 37.0 1000 10:00
Temp. 
S1 30.0 0 00:00
S1 40.0 1000 10:00 M
Speed 
S1 40.0 0 00:00
S1 40.0 800 10:00 M
S1 40.0 0 00:00
S1 40.0 800 10:30M
Start/Stop
Start single temperature procedure
Time 

NOTICE: Press or of Temp will utost rt the instrument to the setting
temper ture. If no pressing or , h ve to press “St rt/Stop” to st rt
oper tion.
d) Press or of eg. to select egment.
et the segment’s values according to
c). Total five segments can be set for operation.
2. How to Set Multi-progr m Connecting
a) Press “Prog.” to connect segment 1, 2, 3,
4, 5 to operate as 1- 2, 1- 2- 3,
1- 2- 3- 4, 1- 2- 3- 4- 5.
NOTICE: Multi-progr m st rt from S1.
b) E.g., to connect programs 1- 2- 3- 4.
Press “Prog.”, LCD display as the right chart.
“ 1” is the starting segment which could not be changed.
“ 2” is the ending segment. Press of eg. till it shows “ 4”.
Then press “Prog.” to confirm the value. “ 1” changes to
“ 14” as the right chart. Multi-program set is 1- 2- 3- 4.
c) Press “ tart/ top” to operate the multi-program 1- 2- 3- 4.
NOTICE: After fix the segment, press “St rt/Stop” to oper te the multi-
progr m.
3. How to Shut off the Temper ture, Speed nd Timing Function
a) Press or of eg. to select one segment from
1, 2, 3, 4, 5.
b) Press of Temp. till it displays “OFF” on the LCD
to shut off the thermo function. imilarly, press of peed
or Time to shut off the shaking or timing function.
PROG to vali ate
Star:S1 En :S2
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Thermo Shaker Incubator SHA71-1600
Prog. 
S1 30.0 0 00:00
S14 60.0 1000 01:00
Seg. 
S2 40.0 0 00:00
S2 70.0 1000 21:00
Start/Stop
Operate the Multi-Program
S1 30.0 0 00:00
S1 OFF 1000 01:00

NOTICE:
1) Shut off timing function, the timing time is ∞. “CON” displ yed on the LCD
in this c se.
2) In multi-progr m oper ting, if timing function is shut off in one segment,
progr m will oper te t th t segment continuously. Keep press “St rt/Stop”
to stop the segment, oper te fter re-timing.
4. Short Mix
Keep press “ hort Mix”, the instrument starts to shake,
release “ hort Mix”, shaking stops.
In the short mix mode, LCD displays as the right chart.
“600RPM” is the shaking speed. “023 ” is operated time,
which means the instrument already operated 23 seconds.
The time is counted in seconds, more than 999 seconds display 999 .
NOTICE:
1) The m ximum short mix speed c n be set ccording to requirement in
the current segment.
2) After multi-progr m complete, the m ximum short mix speed is the
speed of segment S1.
5. Temper ture C libr tion
The temperature of the instrument has been adjusted before it is sold out. If there is deviation
between the actual temperature and the displayed temperature, you can do as follows to
calibrate it.
NOTICE:
1) The instrument h s 3 c libr tion temper ture points to ensure the
ver city. It is line rly djusted on 10 C, 40 C nd 100 C. The temper ture
ccur cy will be within ±0.5C fter temper ture c libr tion.
2) Both the environment l nd the block temper ture should be lower th n
30C when c libr tion.
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Short Mix is running
600RPM 023S

Adjustment Methods:
a) Power on the instrument, it enters into waiting interface. Make sure the current
temperature in display is below 30 C. If the temperature is higher than 30 C, please wait until
it down below 30C.
b) Inject olefin oil into one of block well, and then put a thermometer into this well (the
precision of the thermometer should be 0.1 C and the temperature ball should be absolutely
immerged into the olefin oil in the block well). Adiabatic material is needed on the block to
separate it from the circumstance. (refer to below Figure A)
NOTICE: To ensure the c libr tion precision, re d the ctu l temper ture
v lue fter the temper ture re ches c libr tion points for t le st 20
minutes.
c) Press and of eg. simultaneously when the
instrument is not operating. The program turns to interface
as the right chart. Practical temperature is behind “P:”
which shows 20.5, and the program auto control the
temperature to 10C. At the meantime, the sign“” flickers
ceaselessly. The value behind “AdjTemP” is calibration
temperature.
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Thermo Shaker Incubator SHA71-1600
Thermometer
Adiabatic Board
eating Cover
Olefin Oil
Figure A
P:10.1 ADJ 
A jTemP= 10.0
Block Well
P:20.5 ADJ 
A jTemP= 10.0

When temperature achieves 10C, “ADJ” and “” flicker
ceaselessly together, the value behind “P:” is still practical
temperature.
d) Wait for 20 minutes, the actual temperature of
thermometer is 9.8C. Press or of Temp. to amend the
value behind “AdjTemP” to 9.8. Press “ tart/ top” to
confirm.
Program saves the value.
Temperature rises to 40C automatically.
The sign “” flickers ceaselessly.
e) When practical temperature reaches 40C, “ADJ” and
“” flicker ceaselessly together.
f) Wait for 20 minutes, the actual temperature of
thermometer is 38C. Press or of Temp. to amend the
value of “AdjTemP” to 38.0.
Press “ tart/ top” to confirm.
Program saves the value.
Temperature rises to 100
C automatically.
The sign “” flickers ceaselessly.
g) When practical temperature reaches 100C, “ADJ” and
“” flicker ceaselessly together.
h) Wait for 20 minutes, the actual temperature of
thermometer is 98C. Press or of
Temp. to amend the value behind “AdjTemP” to 98.0.
Press “ tart/ top” to confirm
i) Program turns to interface for operation as the right
chart.
After temperature calibration, the temperature display is
the same as the practical temperature of block.
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P:10.1 ADJ 
A jTemP= 40.0
P：40.1 ADJ 
A jTemP= 40.0
P：40.0 ADJ 
A jTemP= 38.0
Press “Start/Stop”
P:10.1 ADJ 
A jTemP= 9.8
P：100.1 ADJ 
A jTemP= 100.0
P:40.1 ADJ 
A jTemP= 100.0
P：100.0 ADJ 
A jTemP= 98.0
S1 30.0 0 00:00
S1 40.0 1000 2:00
Press “Start/Stop”

·
NOTICE:
1) During temper ture c libr tion, press nd of Seg.
simult neously to c ncel the celibr tion. The system keeps the
former c libr tion.
2) Do not simult neously press nd of Seg. unless c libr te is
re lly needed.
6. Block Repl cement
a) Open the cover and pull out the three screws
which fix the block to the heating board with the
screwdriver.
b) Take out the screws, put out the block from the
main instrument.
c) Take another block, steadily lay it aside
on the heating board. The block installment
holes should aim at the holes in main instrument.
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Thermo Shaker Incubator SHA71-1600

7. Tube St nd Fixing
a) Remove the block according to
“Block Replacement” in above item 6 first.
Push the two bulges at rear of the stand to
corresponding hollows of the base frame.
Refer to the right chart a).
b) Push and press the front part of the stand
till the “PU H” trip locks in the base frame.
Refer to the right chart b).
8. Pl te fixing
Press one long side of the plate into the base frame.
Press another side till the spring locks the plate
as the arrowhead in the right chart c) indicates.
Make sure the plate is fastened.
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05. Failure analy i and trouble hooting
Failure analysis and processing procedures
No. Phenomenon Possible C uses
Processing
Procedure
1
No sign ls on the
displ y when it
is powered on.
No power Check the power
Broken Fuse Exch nge fuse (
250V 3AФ 5x20)
Broken switch Exch nge the switch
Others Cont ct to the seller
2
The ctu l nd
displ yed
temper tures re
quite different.
Broken sensor Cont ct to the seller
3
“OPEN” in the
temper ture
displ y with the
l rm of beep.
Temper ture sensor
is broken or the
environment l
temper ture is below
0C
Cont ct to the seller
4
“SHORT” in the
temper ture
displ y with the
l rm of beep.
Temper ture sensor
is broken or the
environment l
temper ture is below
0C
Cont ct to the seller
5
“ERR” in the
temper ture
displ y with the
l rm of beep.
Temper ture sensor
is broken or the
environment l
temper ture is below
0C Cont ct to the seller
6 No he ring or
cooling
Broken sensor or
broken TE module
Cont ct to the seller
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Thermo Shaker Incubator SHA71-1600