Labwit ZWYC-290A User manual

User’s Manual
This Manual applies to:
Modes: ZWYC-290A
Ultimate-Cell
Stackable Shaking Incubator
Orbital Model
ZWYC-290A

Ultimate-cell Stackable Shaking Incubator V2017.2
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TABLE OF CONTENTS
1. INTRODUCTION 3
2. SAFETY PRECAUTIONS 4
3. ENVIRONMENTAL REQUIREMENTS 6
4. TECHNICAL DATA 7
4.1 Dimensions 7
4.2 Standard Functions 9
4.2.1 Main Unit 9
4.2.2 Door 9
4.2.3 Inner Working Chamber 9
4.2.4 Shaking Control 10
4.2.5 Temperature Control 10
4.2.6 Control Electronics 10
4.2.7 Front Door & Shaking Tray Operation 10
4.2.8 Shaking Speed and Shaking Diameter 10
4.2.9 Specifications 11
5. Preparation and Start-up 12
5.1 Unpacking 12
5.2 Lifting / Handling 12
5.3 Leveling the Units 13
5.4 Preliminary Cleaning 13
5.5 Electrical 13
5.6 Connect the Power Supply 14
5.7 Installation of Flask Clamps (Applies P6022 predrilled tray) 14
5.8 Installation of Sticky Mat (Applies P6023 plain tray) 14
5.9 Set of Capillary Thermostat 15
5.10 Operation of Shaking Diameter Adjustment 15
5.11 Extra For Refrigerated Units 18
5.12 Installation of Direct Steam Humidification System (Option) 18
5.13 Installation of CO2 gas supply hose (Option) 18
6. OPERATING THE UNIT 19
6.1 Home Page Summary 20
6.2 Set Menu Page Summary 22
6.3 FUNC Page Settings 23
6.3.1 Switch of Control Mode - Temperature & Speed 24
6.3.2 Switch of Keep Function-Temperature Holding Function 24
6.3.3 Option Switch of Direct Steam Humidification Function 24
6.3.4 Option Switch of CO2 Gas Control Function 24
6.3.5 Option Switch of Photosynthetic Lighting Control Function 25

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6.4 Operating Temperature and Speed System 25
6.4.1 Operating Under Fixed Value Control Mode 25
6.4.2 Operating Under Programmable Control Mode 27
6.5 Operating Photosynthetic Lighting System 30
6.5.1 Operating Under Fixed Value Control Mode 30
6.5.2 Operating Under Programmable Control Mode 31
6.6 Operating the Humidity System 33
6.7 Operating the CO2 System 34
6.8 Setting Defrosting Cycle 34
6.9 Setting Clock Time 36
6.10 Setting Door Password and Communications 37
6.10.1Door Locking Password Setting 37
6.10.2RS485 Communication Address Setting Option 38
6.11 Using USB Data Logging System 38
6.12 Using UV Light 38
6.13 Using Sticky Mat 39
6.13.1Loading/Unloading the Sticky Mat 39
6.13.2Uninstalling the Sticky Mat 39
6.13.3Adhesive Strength 39
6.13.4Safety 40
7. CALIBRATING THE UNIT 41
7.1 CALIB TEMP—Calibrating the Temperature Display 41
7.2 CALIB HUMI—Calibrating the Relative Humidity Display 42
7.3 CALIB CO2—CO2 Calibrating the CO2 Concentration Display 42
7.4 Restoring Factory Default Settings 43
8. ALARMS 44
9. MAINTAINING THE UNIT 45
9.1 Recommended Daily Maintenance Checks 45
9.2 Recommended Annual Maintenance Checks 45
9.3 Recommended as Required 45
9.4 Replacing the Fuse 45
9.5 Cleaning Cooling Condensers 46
9.6 Cleaning and Replacing the Sticky Mat 46
10. ELECTRONIC CONTROL SYSTEM 47
11. TROUBLE SHOOTINGS 48
12. AFTER-SALE SERVICE 50

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1. INTRODUCTION
LABWIT shaking incubators have achieved a good reputation and trust among various
clients for its advanced temperature controlling technology, sound framework design,
excellent molded exterior and outstanding professional workmanship. These products have
been exported through most leading companies into European, American and East Asian
markets.
The spaces in the labs are spacious and expensive. The ZWYC-290A can be stacked up to
2-3 units high to offer multiplied incubation capacity on a single unit footprint. Stacking also
can be easily managed at a later time. Each compartment unit operates independently with
cooling, TFT touch screen control panel, intelligent precise control of temperature and
shaking speed with "ramp and soak" programs and solid operating safety features with as
standard, and can be upgraded with modular controlling options of, active humidity control,
CO2 concentration controls.
This equipment is specially designed for the research experiment that involves microbiology,
pharmacy and agriculture. It is widely used for cell culturing, hybridization, cell aeration, and
solubility studies, etc.
Since the date of your purchase of this product, after-sale service will always be close to you
through your local dealer and/ or the importing company of your region.
Anyhow, no matter what questions you have using our equipment; please do not hesitate to
contact us.
LABWIT thanks you for your trust in this product!
Safety instruction!
Plea Safety instruction!
Please be sure to follow the instructions, which are really important for your safety.
se be sure to follow the instructions, which are really important for your safety
Prior to operation, this manual should be read thoroughly and completely
understood-as it might be helpful to master the operation techniques of this unit.
LABWIT shall have no obligation and responsibility for any damage on the instrument
caused by users for failing to comply with the operation instruction and the
environmental requirements stated in this specification.
LABWIT is entitled to modify the content of the specification at any time without
appropriate notification.

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2. SAFETY PRECAUTIONS
The machine can be only operated by the trained and authorized person.
The maintenance can be only conducted by trained person, LABWIT or its authorized
agency.
The maintenance spare parts shall be provided by the manufacturer and LABWIT,
and users are not allowed to replace them without authorization.
It’s prohibited to use any materials that may cause overpressure, combustion or
explosion in the equipment. Any uncontrolled cultivation of toxic or pathogenic or-
ganisms is not recommended for this equipment.
The loading volume must not exceed 1/3 of the total volume of the flask.
Reduce the loading or the shaking speed until the equipment runs smoothly, if loud
noises or strong vibrations occur.
Do not expose equipment to inappropriate ambient conditions, such as temperature,
or humidity outside the stated operating range.
The power supply of the incubator shall be well grounded so as to prevent operators
from electric striking.
The electrical supply circuit to the incubator must confirm to all national and local
electric codes. Check the serial-data plate for voltage, cycle, phase and amperage
requirements before you connect the unit.
Do not pull out the plug when the unit is in use. Never drag on the wire to unplug the
unit.
A separate branch circuit is recommended to prevent loss of samples due to over-
loading or failure of other equipment on the same circuit.
A surge protector is recommended to avoid power-related faults.
In case of malfunction or burning smell, the unit must be unplugged immediately .Use
a circuit breaker to cut off the power supply. Continuance of abnormal state will end
up with fire as a result of overheating.
The electric power supply must be cut off in following situations:
When the insulation is damaged.
When opening the door of electrical power box. Opening the top/back cover
without cutting off power supply might result in electric shock.
When replacing the fuse. Replacing the fuse without cutting off the power supply
will probably result in electric shock.
When a malfunction occurs, mishandling will result in the further damage of the
equipment or accidental injury.
If you do not use the unit for a long period of time.
Wear personal safety kits, such as safety glasses, safety gloves, when operating
WARNING!
The section outlines the important safety precautions for both
personnel protection and operation of the equipment.
Negligence of the following instructions may lead to serious
hazards and injury.

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temperature is above 50°C。
Don't open the front door while the equipment is in operation. Make sure that all
parts/shaking tray have stopped moving before opening front door.
Always keep the work place clean and tidy to avoid possible health hazards.

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3. ENVIRONMENTAL REQUIREMENTS
Best working temperature shall be 18°C--30°C, and humidity no more than 80%RH.
In consideration of ventilation, heat dissipation and otherfactors, a minimum of 20 cm
space shall be reserved in the surrounding of the equipment during installation to
facilitate adequate convection of air around the unit.
Indoor use only. Do not locate the unit exposed to direct sunlight or near heating
/cooling ducts.
The incubator shall be placed on a flat, solid and fire-retardant platform which can
support its weight.
The unit must be kept away from electromagnetic interference sources.
There shall be no inflammable, explosive, corrosive, toxic or harmful gas that may
cause sharp temperature change in the vicinity of the equipment.
LABWIT only commits that the incubator can operate normally in the place where the
elevation is less than 2000m.
The power supply voltage of this equipment shall be allowed to change within ±10%
of the rated working voltage.
The transient over-voltage of the power supply shall be Class II.
The pollution degree is Class II. Please keep the chamber clean. Regular cleaning is
required.

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4. TECHNICAL DATA
4.1 Dimensions:
Side view of 3 units stacked with low base with doors closed
Picture. 1 (in mm)

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Side view of 3 units stacked with low base with doors open
Picture. 2 (in mm)

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4.2 Standard Functions
4.2.1 Main Unit
Picture. 3
1 Main Trip Switch 7 Control Panel
2 Illumination Light Switch 8 WiseKonect PlusTM Antenna (Option)
3 UV Light Switch 9 Door Handle
4 Drain Outlet 10 Ventilation Holes
5 Door 11 USB Interface
6 Finger Printer Sensor (Option)
4.2.2 Door
The elements on the door include
Observation Glass Window
Sliding bearing
Tray Support Rails
Illumination Light
4.2.3 Inner Working Chamber
The elements inside the inner working chamber
Stainless Steel Chamber Wall
Shaking Mechanism
Capillary Thermostat

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Ventilation Grilles (with cross flow fans behind the back wall)
PT100 Temperature Sensor (behind the back wall)
UV Sterilization Light (behind the back wall)
4.2.4 Shaking Control
Quin drive shaking system ensures smooth and reliable orbital shaking movement
with a speed range between 30-300rpm. The orbital diameter is adjustable from 1mm
to 50mm continuously. The loading weights must be low the maximum rated, other-
wise, it will prevent the shaking tray from smooth movement, and eventually increase
the wear out the bearings and joints.
4.2.5 Temperature Control
The heaters are behind the back wall. The temperature is controlled with a PT100
temperature sensor. The heat generated is distributed via 2 cross fan evenly across
the inner working chamber. Over temperature protection is in place. The melting fuse
switch off the heating at 85°C. In addition, the capillary thermostat protects the
temperature overheating with adjustable range up to 120°C.
4.2.6 Control Electronics
The microprocessor controller is equipped in the front door. It is designed to service
the precise control requirements of the working chamber environment, providing op-
timum programmable conditions for culture growth. The microcomputer is “us-
er-friendly” with 5.6’’ large touch screen to permit efficient operator entry and settings
of data.
Each stack of unit is operated independently its own controller.
4.2.7 Front Door & Shaking Tray Operation
The shaking tray of the equipment is locked in place while the door is closed. When
the door is fully open, the locking hocks release the shaking tray and in the same time
the releasing switch lifts the shaking tray out of the locking cones on the base tray.
Then the shaking tray can be pulled out over the tray support rails, and the sliding
bearings on the door. Once the shaking tray is pulled out to the end, the shaking tray
can be removed freely.
The shaking movement of the tray is interrupted as soon as the door is opened by
more than 20°of angle. The shaking tray can only be ejected once the shaking tray
stops completely. In the same time, the heater stops working at the same time when
door is opened.
Shaking movement and heating controlling resumes once the door is closed.
DANGER!
MAKE SURE THE SHAKING TRAY IS COMPLETELY
STOPPED BEFORE OPENING THE FRONT DOOR!
Failure of doing so will cause severe damage to the door
locking system, hence door ajar alarm may be activated!

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4.2.8 Shaking Speed and Shaking Diameter
The recommended speed range for different shaking diameters for multi stacking
units are as below,
4.2.9 Specifications
Machine
Shaking Unit
Outside (WxDxH)
1320x938x586mm (Side
Cooling)*
Drive Type
Quin Drive System
Inside (WxDxH)
940x570x420mm
Tray Size
850x450mm
Volume
225L
Maximum Load
25kg
Weight (with Cooling)
200kg
Speed Range
30-300 rpm
Control Panel
TFT Touch Screen
Speed Accuracy
±1 rpm
Illumination
Halogen
Timer
0-9999 mins
Operation Menu in
English
Shaking Mode
Orbital
Ambient Temperature
5-35°C
Shaking Diameter
1-50mm stepless adjustment
UV Light
≥400 mW/m2
Noise Level
<70 dB (1m above floor)
CO2
Power
1150W
Principle of Sensor
Infared, NDIR
Electricity
AC 220-240 Volt, 50/60Hz
CO2 Range
0-20%
Fingerprint System
Option
CO2 Accuracy
±0.15 @ 5.0%
Temperature
Temperature Range
25-55°C
Control Mode
Fixed Value & Program
CO2 Supply Auto
Switch
Option
Temperature Range
Ambient-15 to 60°C (Min. 4°C)#
Temperature Accuracy
0.1°C
Photosynthetic Lighting
Temperature Uniformity
±1.0°C @37°C
Light Type
LED, 50% Red, 50% Blue
Principle of Sensor
PT100
Spectrum
Red: 640-660nm, Blue: 430-450nm
Air Circulation
360m3/Hour
Light Intensity
13,000 Lux (Red: 6500 Lux, Blue:
6500 Lux)
Humidity
Humidity Range
40-80%RH, at 25-55°C
Control
Yes, Individually, from 0-100%
output
Humidity Accuracy
1%RH
Control Mode
Fixed Value & Programmable
Humidity Uniformity
±3%RH
Dimensions (WxDxH)
890x500x10mm
Principle of Sensor
Capacitive
Power
300W
Humidification Mode
Direct Steam Injection
* Without side cooling: 1080*870*590mm
Water Heater
450W
# Min. 15°C when Photosynthetic Lighting option is applied.
DANGER!
In case excessive vibration occurs during operation, stop the operation
immediately, leveling the unit/s and reduce the shaking speed or
shaking diameter.
Negligence of such vibration may cause severe hazards!

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5. PREPARATION AND START-UP
5.1 Unpacking
At delivery, examine the exterior for physical damage while the carrier’s representative is
present. If exterior damage is present, carefully unpack and inspect the equipment and all
accessories for damage. If there is no exterior damage, unpack and inspect the equipment
within five days of delivery. If you find any damage, keep the packing materials and imme-
diately report the damage to the carrier. Do not return goods to the manufacturer without
written authorization. When submitting a claim for shipping damage, request that the carrier
inspect the shipping container and equipment.
5.2 Lifting / Handling
These units are heavy and care should be taken to use appropriate lifting devices that are
sufficiently rated for these loads. Units should only be lifted from their bottom surfaces.
Doors, handles and knobs are not adequate for lifting or stabilization. The units should be
completely restrained from tipping during lifting and transport to prevent shifting and
damage.
The depth of the incubator is 934mm, when moving the incubator through pathway, the door
handle can be removed, so that the depth reduces to 867mm. To do so, please follow the
below instructions,
Open the front door to "half" open position.
DANGER!
Don't use force to open the door to full open position when
the unit is powered off.
Excessive force will cause damage to the door.

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Remove the two caps on the door,
Use the Allen key (not supplied) to unscrew the 2 x M10 Hexagon screws
The door handle can now be removed.
5.3 Leveling the Units
5.3.1 Before starting your equipment, the unit must be placed horizontally on a solid,
flat floor, and elevated and leveled with three foot blocks and one flexible block
(with adjustable bolt). The foot blocks are provided to level as well as “fix”the
unit firmly on the floor. To allocate the blocks in position, please do strictly
follow the below procedures:
5.3.2 Unscrew the safety nut on the flexible block first, screw down the adjusting bolt
of the flexible block, so that it will leave some gap when it fits underneath the
hole on the base.
5.3.3 There is one hole on each corner of base of the unit, locate each of them.
5.3.4 Use lifting tools to elevate the unit, and place the four foot blocks right un-
derneath the holes on each corner.
5.3.5 Lay down the unit, check and adjust the potions of the foot blocks and make
sure the tips of the blocks sits firmly in the holes. Make sure hands off the unit
before the unit is laid down! Do be careful of your hand!
5.3.6 Use a spanner to unscrew the adjusting bolt, raising the height of the flexible
block, until feeling some strength, and the tip of the bolt sits firmly in the hole.
5.3.7 Make sure the unit is elevated leveled and sits firmly on the four blocks. Try
setting the unit at its medium speed, e.g. 150rpm, slowly increase the speed
and adjust the adjusting bolt until no vibration is detected.
5.3.8 Finally screw the safety nut of the flexible block, lock up the adjusting bolt.
5.4 Preliminary Cleaning
Each new equipment was thoroughly cleaned prior to leaving the factory. It is recommended;
however, before installation, LABWIT recommends decontaminate all surfaces within the
interior chamber, outer door and gasket. They can be wiped down with a disinfectant of 70
percent alcohol or similar non-corrosive antimicrobial agent. After cleaning, dry all interior
Safety instruction!
Please be sure to follow the instructions, which are really im-
portant for your safety.

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14
components with a sterile cloth as necessary. Absolutely no chlorinated or halogen
materials are to be used in the chamber.
5.5 Electrical
Each unit is equipped with a trip switch to avoid power-related faults. Whereas, the electrical
supply circuit to the incubator must conform to all national and local electrical codes. Plug
the power plug securely into a grounded power source. VOLTAGE SHOULD NOT VARY
MORE THAN 10% FROM SERIAL PLATE RATINGS. A separate branch circuit is
recommended to prevent possible loss due to overloading or failure of other equipment on
the same circuit.
5.6 Connect the Power Supply
Connect AC power via power wire, turn on the trip switch to start up the equipment after the
connected power supply meets the above stipulated requirements.
5.7 Installation of Flask Clamps (Applies P6022 predrilled tray)
The tray is predrilled with threaded holes for different sizes of Erlenmeyer flask clamps*.
To install the clamps onto the platform tray, please follow the instructions below,
Stop the unit from shaking and pull out the platform tray
Use the screw driver to fix the O clamps with screws onto the platform. (Flask
Clamp≥1000ml, 4 screws at corners, Flask Clamp<1000ml, 1 screw in the center)
Please make sure all screws are securely fastened before turning the unit back on.
* Only use Labwit "O Clamps" for this tray.
Maximum loading capacity with P6022 predrilled tray
Flask (Pcs)
50ml
100ml
250ml
500ml
1000ml
2000ml
3000ml
5000ml
Max capacity
91
70
40
26
15
11
8
5
For Erlenmeyer Flasks only. Flask dimensions may reduce max. capacity
5.8 Installation of Sticky Mat (Applies P6023 plain tray)
The tray is specially designed for use with Labwit Sticky Mats. The Sticky Mat is used for
attaching vessels, such as flasks, bottles, dishes etc onto the shaking platforms for all
shaking equipments, for example, shakers, rockers as well as shaking incubators. It is as
standard 20 x 20 cm in size and can be used to cover the shaking platforms replacing the
traditional flask clamps. The Sticky Mat can be easily removed and repositioned by simply
peeling off the shakingtray surface.With propercare, the StickyMat can be used for several
years.
Before installing the Sticky Mat, clean the surface of the platform tray, making sure the
platform is completely undamaged, clean and oil-free.
Moisturize the platform tray by spray bottle or wet towel.
Remove one of the two plastic sheet covers of the Sticky Mat. Dip the sticky mat with
water.
Install the Sticky Mat onto the platform tray. Use the ruler or hard card to scrape and
squeeze out all the water and bubbles.

Ultimate-cell Stackable Shaking Incubator V2017.2
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Wait for 12 hours and peel off the top sheet cover.
The Sticky Mat is now ready for loading.
Maximum loading capacity with P6023 plain tray with Sticky Mats
Flask
50ml
100ml
250ml
500ml
1000ml
2000ml
3000ml
5000ml
Max Load
98
72
50
32
18
11
8
6
For Erlenmeyer Flasks only. Flask dimensions may reduce max. capacity
5.9 Set of Capillary Thermostat
The capillary thermostat inside the working chamber works as a protection against over
temperature. It has a temperature set range of up to 120°C . It is set at "80°C " as default.
Set the thermostat to 65°C .
Switch on the main unit and press "Set" key
Press the "FIXED"
Press "Temp" to set the temperature to the maximum
working temperature, e.g."40.0"°C
Press "Back" to the home page.
Press "Run" to start the temperature control system
Wait until the displayed temperature in the home page
reaches the set temperature.
Open the door, turn the thermostat knob anti-clockwise
slowly until hearing a clicking sound from the thermo-
stat.
Turn the thermostat knob clockwise 5°C higher.
Now the thermostat is set, and always set the thermo-
stat some °C higher than the maximum working tem-
perature to ensure continuous operation.
5.10 Operation of Shaking Diameter Adjustment
The incubator has an adjustable shaking diameter of Ø1-50mm. To adjust it, follow the
instructions below,
Attention:
Power supply must be disconnected before performing the following
operations!
.

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16
5.10.1 Tools needed: 17*19 spanner, 8*10 spanner, Allen key 6mm, Allen key
8mm
5.10.2 Turn off the unit, open the glass door, pull out and remove the shaking tray,
5.10.3 Use 6mm Allen key to remove the four Allen screws from the base tray.
5.10.4 Lift the base tray and support it with a long object, until seeing the center of
the shaking mechanism (as illustrated)
5.10.5 Use the 8mm Allen key to loosen the (A) central Allen screw.

Ultimate-cell Stackable Shaking Incubator V2017.2
17
5.10.6 Loosen the (C) safety nut with 17*19 spanner
5.10.7 Use the 8*10 spanner to rotate the (B) adjusting bolt clockwise to decrease
the shaking diameter, but anti-clockwise to increase.
5.10.8 When adjustment is done, tighten the (B) adjusting bolt and (A) central Allen
screw.
5.10.9 Install the base tray and shaking tray back in place.
Chart of Ratio Between Speed and Shaking Diameter
Attention:
The maximum shaking speed should decrease when the shaking
diameter is increased. Please see the chart below for the chart of ratio
between speed and shaking diameter.
.

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5.11 Extra For Refrigerated Units
After transport or moving –DO NOT USE this unit for at least 24 Hours, unless you are a
100% sure that the unit has been moved in an upright position only.
If the equipment is continuously used at low temperatures, condensate can occur inside of
the incubator. It is recommended that you wipe condensations out, or-if possible-heat up the
incubator to evaporate this small amount of condensation once every 10 days of operations.
To extend the compressors life and to maintain an excellent performance of your refrigera-
tion system, the condenser of the unit should be cleaned every month.
5.12 Installation of Direct Steam Humidification System (Option)
Before starting operation, the direct steam humidification system must be connected
to unpressurized water tank or a pressurized water source with inlet pressure not
exceed 0.3 bars.
To connect the feed hose to the system, insert the hose into the connector as much
as it goes.
5.13 Installation of CO2 gas supply hose (Option)
The inlet nozzle for CO2 gas supply is on the right side of the case wall. To connect
the CO2 gas supply hose to the nozzle, make sure:
Inlet pressure not exceed 3.0 Bar. (2.0 Bar recommended)
Gas supplied must be dry, clean, dust and oil free.
The hose must remain intact and pressure resistant.
Keep the hose secured by a hose clamp.
ATTENTION:
The nominal cooling capacity can only be reached if the ambient tempera-
ture at the cooling aggregate is below 30 °C.
Clean the shaking incubator before you put it into use and on a regular base.
The interior should be wiped down with an appropriate disinfectant, such
as 70 % ISOPROPYL ALCOHOL or equivalent.
DO NOT USE ANY CHLORINATED OR HALOGEN MATERIAL-
AS THIS IS HARMFUL TO THE POLISHED STAINLESS STEEL!!!
ATTENTION!
Use only distilled or deionized water. Using water outside this range will
void your warranty.

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19
6. OPERATING THE UNIT
Once the equipment is properly installed with all utilities connected, turn on the power switch
on the left of the equipment.
When power is applied, the incubator will first display the "welcome page" and then LCD will
display the "home page" when the system is under working conditions.
Afterwards, user can set parameters via setting menus. After users confirm the setting of
parameters, the incubator can start controlling the general parameters, such as temperature,
speed, as well as the relative humidity (Option) and CO2concentration at the value set by
the user.
Welcome Page
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