LEEC C100 User manual

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USER MANUAL
LEEC CLASSIC INCUBATOR
C100, C157 and C330
CONTENTS
1) GENERAL DESCRIPTION
2) INSTALLATION AND SWITCHING ON INCUBATOR
3) PROGRAMMING TEMPERATURE
4) OVER TEMPERATURE CUT OUT
5) CLEANING & DISINFECTING
6) WARRANTY - PREVENTATIVE MAINTENANCE
V1.0 March 2009
LEEC Limited
Private Road No. 7
Colwick Industrial Estate
Nottingham
NG4 2AJ
Tel: [phone]
Fax: [phone]
E-mail: [e-mail]
Website: www.leec.co.uk

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1.0 GENERAL DESCRIPTION
Model C100 100 litre chamber capacity
Model C157 157 litre chamber capacity
Model C330 330 litre chamber capacity
1.1 CONSTRUCTION, HEATING AND INSULATION
Even heating throughout the entire chamber is ensured by the LEEC designed heating
element. The low wattage element is bonded to the outer surface of the chamber walls
ensuring quick heat conduction and low heating element temperature, only a few
degrees above the incubator operating temperature.
New profiled resistive wire heating elements and high performance insulation ensure
optimum temperature control. All models have gentle fan assisted air circulation. This
ensures even temperature distribution throughout the chamber. Fast temperature
recovery keeps heat loss to a minimum after door opening.
1.2 TEMPERATURE CONTROLLER
A very easy to program electronic PID temperature controller is fitted to the LEEC
Classic Incubator range.
This controller is easy to use and enables quick programming of the incubator chamber
temperature.
In addition, this temperature controller has built in under temperature (low) level alarm
and over temperature (high) level alarm functions. The low and high level alarm limits
are factory set to +/- 2.0 °C above/below programmed temperature respectively after a
delay of 2 minutes.
1.3 INDEPENDENT OVER TEMPERATURE SAFETY CUT OUT
An independent fail safe over temperature safety cut out is fitted to this incubator. The
over temperature cut out is factory set to 40°C, but can be adjusted by the customer,
refer to section 4.3. In the extremely unlikely event of the incubator temperature
controller failing on, the over temperature safety cut out will disconnect the heaters.
This device, once tripped, has to be manually reset. Refer to section 3.6 for instructions
to reset.
1.4 CIRCULATION FAN
The internal fan helps to achieve accurate temperature uniformity throughout the inner
chamber. It also helps to enhance temperature recovery when the chamber is heavily
loaded. The fan is located at the top of the chamber. Air is drawn up through the
chamber, circulated around the full width ducting, and returned to the chamber floor.
The fan also improves temperature recovery after inner glass door opening. A micro
switch disconnects the fan upon door opening to minimise temperature loss to the
chamber environment during door opening.

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2.0 INSTALLATION AND SET UP
2.1 UNPACKING INCUBATOR
Instructions:
Remove the incubator from its protective cardboard packaging.
To do this, carefully undo one side of the cardboard packaging and gently slide the
incubator out.
*Position the incubator in the laboratory, either on the laboratory bench, or under the
bench. It is recommended that if the incubator is to be placed under the laboratory
bench it is raised off the floor sufficiently to reduce the risk of contamination from the
floor entering into the incubator inner chamber.
LEEC manufactures an under bench stand for the Classic Incubator range to raise the
incubator sufficiently off the floor. Please contact LEEC if this is required.
Level the incubator using the 2 independent adjustable feet at the front of the incubator
and secure this position by tightening the locking nut using a 10mm spanner.
It is recommended that a bubble spirit level is placed in the centre position of the
middle shelf in the incubator to check for level from left to right and front to rear.
* Please refer to your employers/organisation Safe Manual Handling Policy: The
Manual Handling Operations Regulations 1992, before attempting to lift the incubators.
The weights are as follows: C100 – 54kg, C157 – 70kg and C330 – 107kg.
2.2 CONNECTION TO THE ELECTRICAL SUPPLY
Your LEEC incubator is supplied with a 2 metre mains cable. Connect the moulded
plug to the 230V 50 Hz mains supply and the IEC connector to the socket at the rear of
the incubator. Please make sure that the IEC connector is pushed in securely.
The mains cable wiring is colour coded as follows: -
•BROWN = LIVE
•BLUE = NEUTRAL
•GREEN / YELLOW = EARTH
Your LEEC incubator requires a 230V 50Hz mains supply.
Once the incubator is situated in the correct position in the laboratory plug the incubator
into a 230V 50Hz mains socket.

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2.4 To switch on the incubator press in the Power button on the front facia panel. This
button will illuminate Green when power is On.
Front Facia Panel Power On
The incubator display will now light up displaying both the programmed temperature
(SET) and actual inner chamber temperature (ACT) and the Heater (H) LED on the
display indicates when the heaters are active. The incubator will now heat up to the
programmed temperature.
2.5 Fuses
A 5 Amp fuse is fitted to the Mains Plug.
At the rear of the incubator there are two internal protection fuses:
A 5 Amp internal fuse. This provides additional protection to the mains plug fuse.
A 3.15 Amp Heater fuse. This provides additional protection to the incubator heating
elements.
2.6 Precautions
A qualified electrician or other competent person must carry out any
electrical work required to install the incubator.
Do not place the incubator in direct sunlight or near a heat source.
Make sure the incubator is not standing on its electrical supply cable.
Before any cleaning or maintenance work is carried out, the mains supply
must be switched off and the plug removed from the electrical socket.

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3.0 PROGRAMMING
3.1 TEMPERATURE DISPLAY
The programmed temperature, Set Point (SET) is displayed in Green and the Actual
Chamber Temperature inside the Incubator Inner Chamber (ACT) is displayed in Red
A Red LED Indicator next to the letter H (Heaters) on the display illuminates when the
heaters are on. When the chamber temperature is very close to programmed set point
temperature, it is normal to see this LED flashing.
A Red LED Indicator next to letter A (Alarm) on the display will illuminate if the
chamber incubator temperature deviates to either the Low or High alarm limits of +/-
2.0°C after a delay of 2 minutes.
3.2 TO PROGRAMME CHAMBER TEMPERATURE:
1. Press the button on the controller once and release
2. SP1 in Flashing Red is now displayed on the screen.
3. Press either the ▲button to increase the set point temperature or the ▼button to
decrease the set point temperature.
Set Point Temperature is displayed in Green
4. Press the button again to return to the normal screen that displays both the
Chamber Temperature in Red and Programmed Set Point in Green
5. *The Temperature can be programmed between a Set Point Temperature
of 20°C to a maximum of 60°C.
*The practical minimum operation temperature of the Incubator is 5°C above
ambient temperature.
The incubator is factory set to operate at +37.0°C unless otherwise specified.
Allow the temperature to stabilise for at least 24 hours before use.

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3.3 HIGH & LOW LEVEL TEMPERATURE ALARM SETTING
The Low Temperature Alarm is factory set to - 2.0°C below the set point (programmed)
temperature.
The High Temperature Alarm is factory set to + 2.0°C above the set point
(programmed) temperature.
NB: There is a dwell period of 2 minutes factory programmed into the controller to allow
sufficient time for the chamber temperature to recover within the low / high alarm set
points; to prevent the alarm from activating immediately after the inner glass door is
closed.
3.4 AUDIBLE ALARM
A Red LED next to letter A (Alarm) on the display will illuminate if the chamber
incubator temperature deviates to either the Low or High alarm limits of
+/- 2.0°C and an audible alarm will activate.
To cancel the audible alarm Press and hold the button for 3 seconds.
If the Low / High alarm values or the 2 minutes dwell need to be adjusted then please
contact LEEC Limited for instructions.
4.0 INDEPENDENT OVER TEMPERATURE SAFETY CUT-OUT
4.1 An independent fail safe over temperature safety cut out is fitted to this incubator. The
over temperature cut out is factory set to 40°C, but can be adjusted by the customer if
higher than 37.0 °C programmed temperatures are programmed. In the extremely
unlikely event of the incubator temperature controller failing on the over temperature
safety cut out will disconnect the heaters. This device once tripped has to be manually
reset, refer to section 3.6 for resetting instructions.
There is an Audible Alarm when the independent over temperature cut out is activated;
the illuminated Green Power On Switch on the front facia panel will change to Red in
the event of the over temperature cut out being activated.
Normal Status
Green
Over T
emperature
Cut Out Activated
Red

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4.2 RESETTING OVER TEMPERATURE CUT-OUT
In the unlikely event of the over temperature safety cut out activating, this can be
reset by Pressing in the Red Button at the Back of the incubator.
Over Temperature Cut Out at Rear of Incubator
Push In Red Button to Reset
4.3 PROCEDURE FOR PROGRAMMING OVER TEMPERATURE CUT OUT IF
OPERATING TEMPERATURE IS ABOVE 37.0°C
If the incubator set temperature is above 37.0°C the procedure to set the over
temperature cut-out is as follows:
1) At the rear of the incubator locate the over temperature cut out adjustment screw,
turn this screw fully clockwise to raise the cut out to maximum
2) Programme new set point temperature on temperature controller. Allow time for the
incubator to stabilise at this new set point temperature.
3) Gently turn the over temperature cut out adjustment screw back (anti-clockwise) until
the incubator over temperature cut out activates. The Power switch on the facia
panel will illuminate RED and audible alarm sounds. Then gently increase the
over temperature cut out by turning the adjustment screw a small increment
clockwise.
4) Press the red Reset button, refer to section 4.2.

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5.0 CLEANING and DISINFECTING
5.1 It is recommended that the exterior of the incubator is kept clean by wiping over with a
non abrasive soft damp cloth using a warm soapy water solution. It is very important to
thoroughly dry the exterior after cleaning
5.2 It is advisable to clean the inner chamber regularly. To clean the inner chamber use a
70% isopropanol (alcohol) 30% distilled water solution. Apply using a sterile cloth so as
not to introduce any contamination and wipe over all the surfaces of the stainless steal
inner chamber.
The inner chambers of LEEC incubators are made from the finest quality stainless
steel. However, corrosion can still result from improper use of fungicides and
bactericides. Never use any Chloride based chemicals for cleaning as this can result in
permanent damage to the stainless inner chamber and this is not covered by LEEC
warranty as no stainless steel is completely resistant to chlorine.
NB: Never use the following substances / chemicals / reagents to clean the stainless
steel inner chamber as permanent damage may result:
Sodium Azide
Aqua Regia
Iodine
Ferric Chloride
Sulphuric Acid
Always avoid any Chlorine based chemicals, including bleach.
6.0 WARRANTY - PREVENTATIVE MAINTENANCE
All LEEC Classic Incubators are supplied with a 1 year manufacturer’s warranty from
the date of installation.
Warranty Card – To register your warranty please fill in the User Guarantee
Registration Card and post to LEEC Limited, or to the Distributor that supplied your
incubator if outside the United Kingdom.
All LEEC incubators are built to the highest standards and all manufacturing processes
are compliant to ISO 9001.
All LEEC incubators are built, fully tested then factory calibrated to UKAS traceable
standards.
This Classic Incubator is built to last for many years, but to ensure this unit continues to
function at its optimum performance levels it is recommended that a Preventative
Maintenance Calibration Contract is purchased from LEEC Limited after the units 1
st
year warranty period.
If the incubator is outside the United Kingdom please contact the Distributor the unit
was purchased from for further information on Preventative Maintenance Contracts.