Luminex Luminex 200 User manual

Luminex®200™
Installation Manual
Lum/nex ®
89-00002-00-167

© LUMINEX CORPORATION, 2001-2006. All rights reserved. No part of this publication may be reproduced,
transmitted, transcribed, or translated into any language or computer language, in any form or by any means
without prior express, written consent of:
LUMINEX CORPORATION
12212 Technology Boulevard
Austin, Texas 78727-6115
U.S.A.
Voice: [phone]
Fax: [phone]
Luminex®200™ Installation Manual
PN 89-00002-00-167 Rev. A
November 2006
Luminex Corporation (Luminex) reserves the right to modify its products and services at any time. This guide
is subject to change without notice. Although prepared to ensure accuracy, Luminex assumes no liability for
errors or omissions, or for any damages resulting from the application or use of this information.
The following are trademarks of Luminex: Luminex, Luminex 100, Luminex 100 IS, Luminex 200, xPONENT,
LabMAP, xMAP, Luminex SeroMAP, LumAvidin, Luminex XYP, Luminex FlexMAP, and Luminex SD. All other
trademarks, including Windows, Cheminert, Pentium, and Dellare trademarks of their respective companies.
The contents of this manual and the associated Luminex software are the property of Luminex and are
copyrighted. Except as specified in the End User License Agreement, any reproduction in whole or in part is
strictly prohibited.
EC REP
Paul. A. Rowden
33 Stapleford Road
Middlesbrough
Cleveland TS39ES
Angleterre
([e-mail])

Standard Terms and Conditions For Use of Product
By opening the packaging containing this product ("Product") or by using such Product in any manner,
you are consenting and agreeing to be bound by the following terms and conditions. You are also agree-
ing that the following terms and conditions constitute a legally valid and binding contract that is enforce-
able against you. If you do not agree to all of the terms and conditions set forth below, you must promptly
return the Product for a full refund prior to using them in any manner.
1. Acceptance - ALL SALES ARE SUBJECT TO AND EXPRESSLY CONDITIONED UPON THE
TERMS AND CONDITIONS CONTAINED HEREIN, AND UPON BUYER'S ASSENT
THERETO. NO VARIATION OF THESE TERMS AND CONDITIONS SHALL BE BINDING
UPON LUMINEX CORPORATION ("LUMINEX") UNLESS AGREED TO IN WRITING AND
SIGNED BY AN AUTHORIZED REPRESENTATIVE OF LUMINEX. For purposes of this agree-
ment, "Seller" shall mean the Luminex authorized reseller that sells the Product to Buyer. Buyer, by
accepting the Product shall be deemed to have assented to the terms and conditions set forth herein,
notwithstanding any terms contained in any prior or later communications from Buyer and whether or
not Seller shall specifically or expressly object to any such terms.
2. Warranties - Any warranty obligations for the Product shall be exclusively provided in writing to
Buyer directly by Seller. LUMINEX MAKES NO WARRANTY WHATSOEVER REGARDING
THE PRODUCT AND LUMINEX SPECIFICALLY DISCLAIMS ALL WARRANTIES, EXPRESS
OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FIT-
NESS FOR A PARTICULAR PURPOSE. NEITHER SELLER NOR LUMINEX SHALL IN ANY
EVENT BE LIABLE FOR INCIDENTAL, CONSEQUENTIAL OR SPECIAL DAMAGES OF ANY
KIND RESULTING FROM ANY USE OR FAILURE OF THE PRODUCT, EVEN IF SELLER OR
LUMINEX HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGE INCLUDING,
WITHOUT LIMITATION, LIABILITY FOR LOSS OF WORK IN PROGRESS, DOWN TIME,
LOSS OF REVENUE OR PROFITS, FAILURE TO REALIZE SAVINGS, LOSS OF PRODUCTS
OF BUYER OR OTHER USE OR ANY LIABILITY OF BUYER TO A THIRD PARTY ON
ACCOUNT OF SUCH LOSS, OR FOR ANY LABOR OR ANY OTHER EXPENSE, DAMAGE OR
LOSS OCCASIONED BY SUCH PRODUCT INCLUDING PERSONAL INJURY OR PROPERTY
DAMAGE UNLESS SUCH PERSONAL INJURY OR PROPERTY DAMAGE IS CAUSED BY
SELLER'S GROSS NEGLIGENCE.
3. Buyer's Use of Product -Buyer agrees that no rights or licenses under Luminex's patents shall be
implied from the sale of the Product, except as expressly provided herein, and Buyer does not receive
any right under Luminex's patent rights hereunder. Buyer acknowledges and agrees that the Product
is sold and licensed only for use with Luminex's standard fluorescently dyed microspheres. Buyer
further acknowledges that the Product have not received approval from the United States Food and
Drug Administration or other federal, state or local regulatory agencies and have not been tested by
Seller or Luminex for safety or efficacy in food, drug, medical device, cosmetic, commercial or any
other use, unless otherwise stated in Seller's technical specifications or material data sheets furnished
to Buyer. Buyer expressly represents and warrants to Seller that Buyer will properly test and use any
Product in accordance with the practices of a reasonable person who is an expert in the field and in
strict compliance with the United States Food and Drug Administration and all applicable domestic
and international laws and regulations, now and hereinafter enacted.

BUYER HEREBY GRANTS TO LUMINEX A NONEXCLUSIVE, WORLDWIDE, UNRE-
STRICTED, ROYALTY-FREE, FULLY PAID-UP LICENSE, WITH THE RIGHT TO GRANT AND
AUTHORIZE SUBLICENSES, UNDER ANY AND ALL PATENT RIGHTS IN INVENTIONS
COMPRISING MODIFICATIONS, EXTENSIONS, OR ENHANCEMENTS MADE BY BUYER
TO THE PRODUCT OR TO THE MANUFACTURE OR USE OF THE PRODUCT ("IMPROVE-
MENT PATENTS"), TO MAKE, HAVE MADE, USE, IMPORT, OFFER FOR SALE OR SELL
ANY AND ALL PRODUCT; EXPLOIT ANY AND ALL METHODS OR PROCESSES; AND
OTHERWISE EXPLOIT IMPROVEMENT PATENTS FOR ALL PURPOSES. NOTWITHSTAND-
ING THE FOREGOING, "IMPROVEMENT PATENTS" SPECIFICALLY EXCLUDES PATENT
CLAIMS CONCEIVED AND REDUCED TO PRACTICE BY BUYER CONSISTING OF METH-
ODS OF SAMPLE PREPARATION, METHODS OF CONJUGATING PRODUCT TO ANALYTES,
THE COMPOSITION OF MATTER OF THE SPECIFIC CHEMISTRIES OF THE ASSAYS
DEVELOPED BY BUYER AND METHODS OF PERFORMING THE ASSAYS (I.E., THE PRO-
TOCOL FOR THE ASSAY).
Buyer has the responsibility and hereby expressly assumes the risk to verify the hazards and to con-
duct any further research necessary to learn the hazards involved in using the Product. Buyer also has
the duty to warn Buyer's customers, employees, agents, assigns, officers, successors and any auxiliary
or third party personnel (such as freight handlers, etc.) of any and all risks involved in using or han-
dling the Product. Buyer agrees to comply with instructions, if any, furnished by Seller or Luminex
relating to the use of the Product and not misuse the Product in any manner. Buyer shall not reverse
engineer, decompile, disassemble or modify the Product. Buyer acknowledges that Luminex retains
ownership of all patents, trademarks, trade secrets and other proprietary rights relating to or residing
in the Product.
4. Buyer's Representations, Release and Indemnity - Buyer represents and warrants that it shall use the
Product in accordance with Paragraph 2, "Buyer's Use of Product," and that any such use of Product
will not violate any law, regulation, judicial order or injunction. Buyer agrees to release, discharge,
disclaim and renounce any and all claims, demands, actions, causes of action and/or suits in law or
equity, now existing or hereafter arising, whether known or unknown, against Seller and Luminex,
and their respective officers, directors, employees, agents, successors and assigns (collectively the
"Released Parties"), with respect to the use of the Product. Buyer agrees to indemnify and hold harm-
less the Released Parties from and against any suits, losses, claims, demands, liabilities, costs and
expenses (including attorney, accounting, expert witness, and consulting fees) that any of the
Released Parties may sustain or incur as a result of any claim against such Released Party based upon
negligence, breach of warranty, strict liability in tort, contract or any other theory of law or equity
arising out of, directly or indirectly, the use of the Product or by reason of Buyer's failure to perform
its obligations contained herein. Buyer shall fully cooperate with the Released Parties in the investi-
gation and determination of the cause of any accident involving the Product which results in personal
injury or property damage and shall make available to the Released Parties all statements, reports,
recordings and tests made by Buyer or made available to Buyer by others.
5. Patent Disclaimer - Neither Seller nor Luminex warrants that the use or sale of the Product will not
infringe the claims of any United States or other patents covering the product itself or the use thereof
in combination with other products or in the operation of any process.

End-User License Agreement (EULA) for Luminex® Software
This Luminex End-User License Agreement ("EULA") is a legal agreement between you (either an individual or a
single entity, also referred herein as "you") the end-user and Luminex Corporation ("Luminex") regarding the use of
the Luminex software product provided to you above, which includes computer software and online or electronic
documentation and may include associated media and printed materials (if any) ("SOFTWARE PRODUCT" or
"SOFTWARE").
The SOFTWARE PRODUCT is protected by copyright laws and international copyright treaties, as well as other
intellectual property laws and treaties. The SOFTWARE PRODUCT is licensed, not sold.
1. GRANT OF LICENSE. Subject to the terms and conditions of this EULA, Luminex hereby grants to you a
nonexclusive, nontransferable, nonassignable license (without right to sublicense) under Luminex's copyrights
and trade secrets to use the SOFTWARE PRODUCT on a single computer running with a Luminex instrument/
purchased from Luminex or a Luminex partner pursuant to Luminex's terms and conditions of sale. You may
make one (1) copy of the SOFTWARE PRODUCT for backup or archival purposes only. You may also install the
SOFTWARE PRODUCT on up to three (3) additional computers for purposes of performing ancillary tasks (i.e.
preparing templates/protocols, performing further analysis or re-running previous data), provided such computers
are at a single location and are NOT connected with a Luminex instrument. In addition, You may purchase the
right to use the SOFTWARE PRODUCT on additional computers, as agreed to in writing with Luminex or its
authorized reseller, for purposes of performing ancillary tasks (i.e. preparing templates/protocols, performing
further analysis or re-running previous data), provided such computers are at a single location and are NOT
connected with a Luminex instrument Although no rights or licenses under any of Luminex's patents are granted
by or shall be implied from the license of the SOFTWARE or the sale of Luminex instrumentation to you, the
purchaser, you may obtain a license under Luminex's patents, if any, to use this unit of Luminex instrumentation
with fluorescently labeled microsphere beads authorized by Luminex by purchasing such beads from Luminex or
an authorized Luminex reseller.
2. RESTRICTIONS.
• Each SOFTWARE PRODUCT must only be installed and operated on a single Luminex instrument/
computer.
• You may not use this SOFTWARE PRODUCT for any commercial purpose, including in the
performance of testing services, unless expressly agreed to in writing by Luminex or as authorized
in writing by Luminex through an authorized reseller of the SOFTWARE PRODUCT.
• You may only use the SOFTWARE PRODUCT with microspheres manufactured by Luminex or
with kits developed, manufactured and distributed by licensees authorized in writing by Luminex.
• You must maintain all proprietary notices on all copies of the SOFTWARE PRODUCT.
• You may not distribute copies of the SOFTWARE PRODUCT to third parties.
• You may not reverse-engineer, decompile, disassemble, or otherwise attempt to derive source
code from the SOFTWARE PRODUCT.
• You may not copy (other than one backup or archival copy), distribute, sublicense, rent, lease,
transfer or grant any rights in or to all or any portion of the SOFTWARE PRODUCT.
• You must comply with all applicable laws regarding the use of the SOFTWARE PRODUCT.
• You may not modify or prepare derivative works of the SOFTWARE PRODUCT.
• You may not use the SOFTWARE PRODUCT in a computer-based service business or publicly
display visual output of the SOFTWARE PRODUCT.
• You may not transmit the SOFTWARE PRODUCT over a network, by telephone, or electronically
by any means.
3. TERM AND TERMINATION. Your rights under this EULA are effective until termination. You may terminate this
EULA at any time by destroying the SOFTWARE PRODUCT, including all computer programs and
documentation, and erasing any copies residing on your computer equipment. Luminex may terminate this EULA
upon thirty (30) days written notice to you. Your rights under this EULA automatically terminate without further
action on the part of Luminex if you do not comply with any of the terms or conditions of this EULA. Upon any

termination of this EULA, you agree to destroy the SOFTWARE PRODUCT and erase any copies residing on
your computer equipment.
4. RIGHTS IN SOFTWARE. All rights and title in and to the SOFTWARE PRODUCT and any copies thereof are
owned by Luminex or its suppliers. This EULA is not a sale and does not transfer to you any title or ownership
interest in or to the SOFTWARE or any patent, copyright, trade secret, trade name, trademark or other
intellectual property right therein. You shall not remove, alter, or obscure any proprietary notices contained on or
within the SOFTWARE and shall reproduce such notices on any back-up copy of the SOFTWARE. All title and
intellectual property rights in and to the content which may be accessed through use of the SOFTWARE
PRODUCT is the property of the respective content owner and may be protected by applicable copyright or other
intellectual property laws and treaties. This EULA grants you no rights to use such content.
5. EXPORT RESTRICTIONS. You agree that you will not export or re-export the SOFTWARE PRODUCT to any
country, person, entity, or end-user subject to U.S.A. export restrictions. You hereby warrant no state or federal
agency has suspended, revoked, or denied your export privileges.
6. NO WARRANTY. THE SOFTWARE PRODUCT IS LICENSED "AS IS." ANY USE OF THE SOFTWARE
PRODUCT IS AT YOUR OWN RISK. THE SOFTWARE PRODUCT IS PROVIDED FOR USE ONLY WITH
LUMINEX PRODUCTS. TO THE MAXIMUM EXTENT PERMITTED BY APPLICABLE LAW, LUMINEX AND ITS
SUPPLIERS DISCLAIM ALL WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED
TO, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, AND
NONINFRINGEMENT.
7. LIMITATION OF LIABILITY. IN NO EVENT SHALL LUMINEX OR ITS SUPPLIERS BE LIABLE FOR ANY
SPECIAL, INCIDENTAL, INDIRECT, OR CONSEQUENTIAL DAMAGES WHATSOEVER (INCLUDING,
WITHOUT LIMITATION, DAMAGES FOR LOSS OF BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS
OF BUSINESS INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF OR
INABILITY TO USE THE SOFTWARE PRODUCT, EVEN IF LUMINEX HAS BEEN ADVISED OF THE
POSSIBILITY OF SUCH DAMAGES.
MISCELLANEOUS. This EULA is governed by the laws of the State of Texas, U.S.A., without reference to conflicts of
laws principles. You shall not assign or sublicense or otherwise transfer the rights or license granted hereunder, by
agreement or by operation of law, without the prior written consent of Luminex, and all assignments in violation of this
prohibition shall be null and void. This EULA is the complete and exclusive agreement of Luminex and you and
supersedes all other communications, oral or written, relating to the subject matter hereof. No change to this EULA
shall be valid unless in writing and signed by the party against whom enforcement is sought. The waiver or failure of
Luminex or you to exercise in any respect any right or rights provided for herein shall not be deemed a waiver of any
further right hereunder. If any provision of this EULA is held unenforceable, the remainder of this EULA will continue
in full force and effect.
89-30000-00-122, Rev. A

i
About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
The Luminex 200 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Hardware. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
xMAP Technology Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Required Laboratory Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Luminex Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Safety Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Warnings and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
FCC Label. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Luminex 200 Analyzer Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Barcode Reader Laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Mechanical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Biological . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Heat. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Blue Indicator Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Luminex Website . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Luminex User Manuals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Luminex 200 System Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Connect the Luminex 200 Analyzer and Luminex XYP to
the PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Connect Sheath and Waste Containers. . . . . . . . . . . . . . . . . . . 12
Install the Luminex XYP Instrument Sample Probe . . . . . . . . 12
Adjust the Sample Probe Vertical Height. . . . . . . . . . . . . . . . . 13
Install the Luminex XYP Instrument Reservoir. . . . . . . . . . . . 14
Calibrate and Verify the System. . . . . . . . . . . . . . . . . . . . . . . . 14
Install the SD System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Install the Luminex XYP Instrument Heater Block . . . . . . . . . 17
Network Installation Advisory . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Prepare System for First Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Equipment Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Installation Drawing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Contents

ii
Luminex 200 Installation Manual

1
About This Manual This manual provides installation procedures for hardware and online
documentation. A Luminex®Certified Installer performs initial
system installation for new systems.
The manual’s text and figures offer examples when necessary.
Procedures are presented as step-by-step instructions.
The conventions in this manual assume that the reader has a basic
familiarity with computers, xMAP®technology, and a knowledge of
Microsoft®Windows®software.
The Luminex 200
System The Luminex 200TM benchtop system consists of the Luminex 200
analyzer, Luminex software, computer, monitor, keyboard, mouse,
Luminex XY Platform instrument (Luminex XYP™), Luminex
Sheath Delivery System (Luminex SD™), barcode reader, sheath and
waste containers, and xMAP technology reagents.
Hardware The Luminex 200 system includes the following hardware:
• Luminex 200 analyzer
• Computer (PC), monitor, and accessories
• Luminex XYP instrument
• Luminex Sheath Delivery System (Luminex SD™)
• Power cables
• Two long sample probes
• Reservoir
•Shield
•Heaterblock
• Sheath fluid container
• Waste container
• Sheath fluid line
• Air line
• Sheath fluid intake line
• Communications: 1 serial communication cable
• Communications: 1 USB communication cable
• Communications: 1 CANBUS communication cable
• Barcode reader
• Sample probe height alignment kit
• 3/32 Hexdrive, Balldriver wrench

Luminex 200 Installation Manual
2
xMAP Technology
Reagents • Classification calibration microspheres (CAL1)
• Reporter calibration microspheres (CAL2)
• Classification control microspheres (CON1)
• Reporter control microspheres (CON2)
• xMAP sheath fluid
Required Laboratory
Reagents The following items are necessary to run the Luminex 200 system.
They are not provided by Luminex.
• Household bleach
• 70% isopropanol or 70% ethanol
• Mild detergent
• Distilled water
Luminex Software Luminex software provides complete control of the system and
performs data analysis. The PC accompanying your system is
preloaded with the Luminex software. However, we supply a
software CD should you need to reinstall the software.
This software requires a dedicated system. Unauthorized additional
software is prohibited and may result in improper operation of the
system.
Safety Symbols Please become familiar with the information in this chapter before
installing the equipment. Do not perform procedures on your
Luminex 200 system that are not specifically contained in this
manual, unless you are directed to do so by Luminex Technical
Support.
These symbols describe warnings, cautions, and general information
used in the operation of this instrument. Some of these symbols are
further defined under “Safety Precautions.”

Luminex 200 Installation Manual
3
Warnings and Notes Informational notes and warnings appear in this manual.
Note: A note provides general helpful information. No safety or
performance issues are involved.
Caution: This message is used in cases where the hazard is minor or
only potential hazard is present. Failure to comply with the caution
may result in potentially hazardous conditions.
Warning: This message is used in cases where danger to the
operator or to the performance of the instrument is present. Failure to
comply with the warning may result in incorrect performance,
instrument failure, invalid results, or hazard to the operator.
Danger: This message is used in cases where significant risk of
serious injury or death is present.
Symbol Description Symbol Description Symbol Description
Alternating current
(ac)
Warning
(refer to manual) Warning (refer to manual)
Protective ground Warning (refer to manual) Warning (refer to manual)
On Warning (refer to manual) Warning (refer to manual)
Off Catalog Number Consult instructions for use
In vitro Diagnostic
Medical Device Batch Code Serial Number
Temperature
Limitation Expiration Date Date of Manufacture
Manufacturer Underwriter’s
Laboratory Canadian Underwriter’s
Laboratory
European Union
Conformity WEEE - Do not throw in trash
US

Luminex 200 Installation Manual
4
Safety Precautions Read the following safety information before setting up or using the
Luminex 200 system. A user should be present during operation.
This system contains electrical, mechanical, and laser components
which, if handled improperly, are potentially harmful. In addition,
biological hazards may be present during system operation.
Therefore, we recommend that all system users become familiar with
the specific safety advisories below, in addition to adhering to
standard laboratory safety practices. The protection provided by the
equipment may be impaired or the warranty voided if the system is
used in a manner not specified by the instructions or by Luminex
Corporation. Various informational labels appear on the back of
Luminex system components. Refer to the Luminex System User
Manual for more details about the labels.
This caution label appears on the back of the Luminex 200 analyzer
and on the Luminex XYP instrument.
.
Figure 1. Fuse Caution Label
Do not perform any maintenance or cleaning of the system’s
electrical components, with the exception of replacing fuses.
This label appears on the back panel of the Luminex 200 analyzer,
the Luminex XYP instrument, and the Luminex SD system
Figure 2. CE Label
The Luminex 200 analyzer, XYP instrument, and SD system comply
with European Union (EU) safety requirements and, therefore, may
be marketed in the Europe Single Market.
The Luminex 200 analyzer, XYP instrument, and SD system have
been tested by Underwriter Laboratories, Inc.® (UL).

Luminex 200 Installation Manual
5
This voltage label appears on the back of the Luminex 200 analyzer:
Figure 3. Luminex 200 Serial Number Label
This label appears on the back of the Luminex XYP instrument.
Figure 4. Luminex XYP Serial Number Label
FCC Label This equipment has been tested and found to comply with the limits
for a Class B digital device, pursuant to part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against
harmful interference in a residential installation. This equipment
generates, uses and can radiate radio frequency energy and, if not
installed and used in accordance with the instructions, may cause
harmful interference to radio communications. However, there is no
guarantee that interference will not occur in a particular installation.
If this equipment does cause harmful interference to radio or
television reception, which can be determined by turning the
equipment off and on, the user is encouraged to try to correct the
interference by one or more of the following measures:
• Reorient or relocate the receiving antenna.
• Increase the separation between the equipment and the
receiver.
• Connect the equipment into an outlet on a circuit different
from that to which the receiver is connected.
• Consult the dealer or an experienced radio/TV technician for
help.

Luminex 200 Installation Manual
6
The following label appears on the back of all system components:
Figure 5. FCC Label
Fluidics In the event of a fluid leak, turn off all power to the system and
disconnect all power cords.
Monitor waste levels manually. Do not allow the waste container to
overflow! Empty the waste container each time you fill the sheath
fluid container. Do not place the waste container on top of the
instrument.
Luminex 200 Analyzer
Laser The Luminex 200 system classifies per FDA 21 CFR 1040.10 and
1040.11 as a Class II laser product consisting of a Class I laser
product (Luminex 200 analyzer) and a Class II laser product
(barcode reader).
Figure 6. Laser Product Class Label
Figure 7. Laser Radiation Caution Label
Warning: If biological samples are tested with the system, use
standard laboratory safety practices when handling system waste.

Luminex 200 Installation Manual
7
Under NO circumstances should you remove the Luminex 200
analyzer cover! When performing routine maintenance, turn power
to the Luminex 200 analyzer OFF and the disconnect the power cord.
All laser apertures are located within the Luminex 200 analyzer and
are contained within a protective housing.
This label appears on the optics cover within the Luminex 200
analyzer.
Figure 8. Laser Class Label
This label appears above the laser apertures located inside the optics
enclosure inside the Luminex 200 analyzer.
Figure 9. Avoid Exposure Label
Barcode Reader Laser Do not stare into the beam or shine it into other people’s eyes. This
label is attached to the barcode reader.
Warning: Use of controls or adjustments or performance of proce-
dures other than those specified herein may result in hazardous
radiation exposure.
Attention — L’utilisation des commandes ou réglages ou
l’exécution des procédures autres que celles spécifiées dans les
présentes prescriptions peuvent entraîner d’une exposition à un
rayonnement dangereux.

Luminex 200 Installation Manual
8
Figure 10. Barcode Reader Laser Label
Mechanical
Biological
Heat
Blue Indicator Light The blue light above the Luminex 200 analyzer sample arm indicates
the on/off status of the analyzer; it is harmless. The blue light-
emitting diode (LED) does not emit light in the UV spectrum.
Warning: During operation, this system contains exposed, mov-
ing parts. Risk of personal injury is present. Observe all warnings
and cautions.
Warning: During operation, this system contains exposed, mov-
ing parts which could result in puncture hazard. Risk of personal
injury is present. Keep hands and fingers away from the Luminex
XYP instrument slot during operation.
Warning: During operation, this system contains exposed, mov-
ing parts which could result in pinch point hazard. Risk of per-
sonal injury is present. Keep hands and fingers away from the
Luminex XYP instrument slot during operation.
Note: Access doors must be
closed while operating the
Luminex 200 analyzer; the
operator must be present
during operation.
Warning: Human and animal samples may contain biohazardous
infectious agents. Where exposure (including aerosol) to potentially
biohazardous material exists, follow appropriate biosafety proce-
dures and use personal protective equipment, such as gloves, gowns,
laboratory coats, face shields, or mask and eye protection, and venti-
lation devices.
Observe all local, state, and federal biohazard handling regulations
when disposing of biohazardous waste material.
Warning: The heater plate of the Luminex XYP instrument may be
hot and could cause personal injury if touched. Do not touch the
heater plate.

Luminex 200 Installation Manual
9
Technical Support Luminex Technical Support representatives are ready to help you,
particularly when the system or software cause any questions or
problems. If the question or problem relates to materials from the
assay kit, you should contact the kit provider directly.
Luminex Technical Support is available to users in the U.S. and
Canada by calling 1-877-785-BEAD (-2323). Users outside of the
U.S. and Canada can contact us at [phone]. Inquiries may
also be sent by email to [e-mail].
Luminex Website Additional information is available on the Luminex website. Search
on the desired topic or navigate through menus. Also, review the
website’s FAQ section. Enter http://www.luminexcorp.com in your
browser’s address field. Click Support>Support Login to log into
the Support FAQ site, then click on the Support tab.
Luminex User
Manuals User manuals for Luminex software and hardware are available
online in PDF format. To download a manual, enter http://
www.luminexcorp.com in your browser’s address field. Click
Support>Support Login to log into the Support FAQ site, then click
on the Support tab. You can perform a search using the search
engine in the browser for the manual, or scroll down through the
Title menu to find the manual you want to download. Manuals are
available in English, Spanish, Italian, French, German, and Chinese.
Luminex 200
System Setup Before connecting the system components, ensure that the facility
complies with all system and safety requirements. Read the safety
information that begins on page 3. Position the instrument to
minimize temperature fluctuations.
The following sections describe how to connect the Luminex 200
system components. An Installation Drawing (page 20) provides
clearances and other related information. Figure 11 shows the
components of the system and how they should be placed.
Perform the following procedures to set up the system. Each of these
steps can be found on the page number shown in parentheses:
1. Connect the Luminex 200 Analyzer and Luminex XYP to the PC
(page 10).
2. Connect Sheath and Waste Containers (page 12).
3. Install the Luminex XYP Instrument Sample Probe (page 12).

Luminex 200 Installation Manual
10
4. Adjust the Sample Probe Vertical Height (page 14).
5. Install the Luminex XYP Instrument Reservoir (page 15).
6. Calibrate and Verify the System (page 15).
7. Install the SD System (page 15).
8. Install the Luminex XYP Instrument Heater Block (page 18).
Figure 11. Luminex 200 System Setup
Connect the Luminex 200
Analyzer and Luminex
XYP to the PC
You must set up and calibrate the Luminex 200 analyzer and
Luminex XYP instrument before you can install the Luminex SD
system.
To connect and power on the system:
1. Install the PC and monitor according to the instructions provided
by the PC manufacturer. Place the monitor on top of the PC.
2. Unpack the Luminex 200 analyzer and the Luminex XYP
instrument. Review Hardware on page 1 to verify that each
system component came with the system.
3. Place the Luminex XYP instrument on a clean, flat surface to the
left of the PC.
4. Remove the blue shipping pin from the XYP. Leave the black
knob in the Luminex XYP instrument.
5. Place the Luminex 200 analyzer onto the Luminex XYP
instrument, aligning the black knob on the XYP with the hole in
the adapter plate on the bottom front of the analyzer.
1. Monitor 4. Barcode Scanner
2. PC 5. Luminex SD system
3. Luminex XYP Instrument 6. Luminex 200 Analyzer
1
2
6
5
4
3
Note: Do not place the PC or
monitor on top of the
Luminex 200 analyzer or
XYP instrument.

Luminex 200 Installation Manual
11
6. Ensure that the power switches on the analyzer and XYP
instrument are in the off position.
7. Attach the power cord to the power input module of the Luminex
XYP instrument and attach the serial cable to the Luminex XYP
instrument. Do not plug the Luminex XYP instrument into the
power outlet.
8. Attach the power cord to the input module of the Luminex 200
analyzer, then attach the USB cable (PN 85-10011-00-046) to P1
on the analyzer. Do not plug the power cord into the power
outlet. See Figure 12. Device disconnects for the Luminex 200,
Luminex XYP, and the Luminex SD are the power cords. Do not
position these devices such that it is difficult to operate the
disconnect device.
9. Connect the Luminex 200 analyzer USB cable and the Luminex
XYP instrument serial cable to the PC. See items 11 and 13 in
Figure 12.
10. Connect the barcode reader USB cable, keyboard USB cable and
mouse to the PC.
Figure 12. Luminex 200 Connections (Luminex 200 Analyzer, Luminex
XYP Instrument, and PC)
Note: The cable that connects
the analyzer to the PC is 5 feet
long; the cable that connects the
SD to the analyzer is 2.5 feet
long.
11
13
43
7
2
6
5
1
12
9
10
14
8
1. USB Communication Cable (P1) 8. SD Cable (P2)
2. Luminex XYP Instrument 9. Mouse Cable port
3. Luminex XYP Instrument to PC Serial Cable 10.PC Power Cable
4. Power Cords 11. USB ports
5. Luminex XYP Instrument Power Switch 12. Monitor cable port
6. Luminex 200 Analyzer Power Switch 13. Luminex XYP port
7. Luminex 200 analyzer 14. Printer cable port

Luminex 200 Installation Manual
12
11. Plug the Luminex XYP instrument, Luminex 200 analyzer, PC,
and monitor power cords into approved outlets. We strongly
recommend using an uninterruptible power supply (UPS) to
protect the system from power variations. Choose one that can
provide 1050 Watts for at least 45 minutes. The UPS should be
UL listed, CSA certified, and CE marked when used
internationally.
Connect Sheath and
Waste Containers To connect the sheath and waste containers
1. Connect the color-coded tubing connectors to the Luminex 200
analyzer. Blue = sheath, green = air, orange = waste.
2. Fill the sheath bottle with sheath fluid to just below the air intake
fitting.
3. Place the sheath and waste containers to the left side of the
analyzer, on the same surface as the Luminex XYP instrument.
4. Connect the blue connector to the sheath (bottom) fitting on the
sheath bottle. Connect the green connector to the air (top) fitting
on the sheath bottle. Connect the orange connector (middle) to
the waste fitting on the waste bottle. Verify that the waste tubing
is not elevated more than 12 inches above the Luminex 200. To
maintain a stable flow rate, do not move the waste container or
waste line during system operation.
Install the Luminex XYP
Instrument Sample Probe To install the Luminex XYP instrument sample probe:
1. Verify that the Luminex 200 analyzer and Luminex XYP
instrument power switches are off.
2. Remove the light housing directly above the sample arm by
grasping and firmly pulling out. It remains attached by a wire
harness. Place it on top of the analyzer.
3. Unscrew the cap from the Cheminert fitting. See Figure 13.
Note: Do not touch the Gore-
TexTM seal in the waste bottle
cap or allow it to become wet,
as venting may become
impaired