MBS NextGenPCR User manual

NextGenPCR™
THERMAL CYCLER | INSTRUCTIONS FOR USE
REVISION 2.0
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Important: Read these instructions for use. Failure to read, understand and follow the instructions in this document may result in damage to the
unit, injury to operating personnel, or poor device performance. Contact your local distributor or a Molecular Biology Systems technical specialist
if there are any doubts regarding the proper use of your system.
CAUTION! 
Revision History
Version Date Description
1.0 08/02/2020 Development of the IFU – intial version
2.0 09/28/2023 Following parts of the document were updated: Safety symbols,
Contact information, Product information, Safety, Footer/Header;
Manufacturer registered place of business:
Molecular Biology Systems, B.V
Diazweg 7, 4462 HG Goes, The Netherlands
T: [phone]
For Research Use Only. Not for use in diagnostic procedures.
For Professional Use Only.
is a registered trademark of Molecular Biology Systems, B.V.
All other trademarks are the sole property of their respective owners.

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NextGenPCR™ Thermal Cycler - Instructions for use, v2.0, October 2023
INSTRUCTIONS FOR USE
NextGenPCR™
THERMAL CYCLER
PREFACE 4
1. WARRANTY 7
2. PRODUCT INFORMATION 10
3. OPERATION SAFETY 16
4. ELECTRICAL SAFETY 19
5. GETTING STARTED 20
6. OPERATING THE INSTRUMENT’S FIRMWARE 22
7. MICROPLATE PREPARATION 28
8. DEVICE COMPATIBILITY 30
9. TROUBLESHOOTING 31
10. MAINTENANCE AND SERVICING 33

PREFACE
Introduction
Welcome to the user manual for the NextGenPCR™ Thermal Cycler! We are thrilled to introduce you to our

technology, intuitive controls, and unparalleled performance, the NextGenPCR™ Thermal Cycler is engineered to
meet the demands of both novice researchers and seasoned professionals alike.


genetic analyses, the NextGenPCR™ Thermal Cycler is your ideal companion for achieving reliable and reproducible
results. Let’s embark on this exciting journey to unlock the potential of your research together!
How to use this manual

the NextGenPCR™ Thermal cycler in molecular biology applications. The manual provides information on the machine’s

machine, including power requirements and environmental conditions. Step-by-step procedures are outlined for
operating the machine, such as programming PCR protocols and adjusting temperature and time parameters.
The manual includes guidelines for initial device setup, creating and optimizing PCR programs, covering

Maintenance guidelines address routine cleaning, calibration, and the replacement of consumables.

assistance is provided. Safety considerations are emphasized.
IMPORTANT! Before operating the NextGenPCR™ Thermal Cycler, carefully read and familiarize yourself with
the user manual provided by the manufacturer. Understand the device’s capabilities, limitations, and safety
precautions. Ensure that anyone using this product has received instructions in general safety practices for
laboratories and the safety information provided in this document.
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NextGenPCR™ Thermal Cycler - Instructions for use, v2.0, October 2023

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NextGenPCR™ Thermal Cycler - Instructions for use, v2.0, October 2023
Safety alert words
There are four safety symbols used in this document at points where the user shall be aware of relevant hazards.
Each safety word implies a particular level of observation or action.
IMPORTANT! Relates to important information or instructions that are not directly related to safety hazards but

schedules, special considerations.
CAUTION! Indicates a potential hazard or unsafe practice that, if not avoided, could result in minor to

carefully to prevent accidents.
WARNING! Indicates a potential hazard or unsafe practice that, if not avoided, could cause serious injury,

guidelines and precautions.
DANGER! Is a strong and urgent safety word used to indicate an immediate and severe risk that, if not

immediate attention and adherence to safety procedures.
Reporting
IMPORTANT! Any serious incident that occurs in relation to the device shall be reported to the manufacturer
and the Member State in which the user is established.
How to obtain support and more information
Resource How to contact
Local NextGenPCR™ Find our representative in your country on our nextgenpcr.com website.
representatives If no local representative is present in your country, you can contact us
directly by emailing us at [e-mail]
Technical notes and literature Technical notes and literature can be found on www.nextgenpcr.com
Technical Specialist Contacting our technical specialist is possible by emailing [e-mail]
Customer support E: [e-mail] | T: [phone]