Metertech SP-8001 Quick start guide

Provide Accurate Results
Operational Manual
UV/Visible Spectrophotometer
SP-8001
Metertech Inc.
Version 1.2

Metertech Inc. provides this publication “as is” without warranty of any kind, either express or implied,
including, but not limited to the implied warranties of merchantability or fitness for aparticular purpose.
All rights are reserved, including those to reproduce this publication or parts thereof in any form without
permission in writing from Metertech Inc.
Information in this publication is subject to change without notice and does not represent acommitment on
the part of the vender.
Any errors or omissions which may have occurred in this publication despite the utmost care taken in its
production will be corrected as soon as possible, but not necessarily immediately upon detection.
Copyright© Metertech Inc. 1999
Metertech Inc. ‧63-2 Cheng Gong Rd. Sec. 1‧Nangang, Taipei, Taiwan, R.O.C.
E-Mail: [e-mail] ‧Web Site: www.metertech-inc.com
Tel.: [phone] ‧Fax.: [phone]

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Contents
2
Chapter 1 Introduction
--------------------
4
§1-1 Specification
--------------------
4
§1-2 Safety Notes
--------------------
5
Chapter 2 Installation
--------------------
6
§2-1 Unpacking
--------------------
6
§2-2 Installation
--------------------
7
Chapter 3 General
--------------------
8
§3-1 User Interface
--------------------
8
§3-2 General Function Description
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10
§3-3 Initialization
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12
Chapter 4 System setup
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14
§4-1 System Setup Menu
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14
Chapter 5 Photometric
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19
§5-1 Photometric Parameter Setup
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19
§5-2 Photometric Function Structure
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20
§5-3 Basic Photometric Measurement
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20
Chapter 6 Spectrum
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24
§6-1 Spectrum Parameter Setup
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24
§6-2 Spectrum Function Structure
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25
§6-3 Basic Spectrum Measurement
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26
Chapter 7 Timescan
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31
§7-1 TimeScan Parameter Setup
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31
§7-2 TimeScan Function Structure
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32
§7-3 Basic TimeScan Measurement
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33
Chapter 8 Kinetic
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35
§8-1 Kinetic Parameter Setup
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36
§8-2 Kinetic Function Structure
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37
§8-3 Basic Kinetic Measurement
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38
Chapter 9 Quantitative
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40
§9-1 Quantitative Parameter Setup
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40
§9-2 Quantitative Function Structure
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41
§9-3 Basic Quantitative Measurement
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42
Chapter 10 Accessories
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45
§10-1 Multi-Cell Holder
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45
§10-2 Sipper
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47
Chapter 11 Maintenance
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49
§11-1 Routine Maintenance
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49
§11-2 Replacement of Lamps
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50
Appendix A Thermal Controller Operation
Instruction
--------------------
52
Appendix B Sipper Operation Instruction
--------------------
54
Appendix C Setting of USB Serial Port
--------------------
57

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Chapter 1 Introduction
The SP-8001 UV-VIS Spectrophotometer is an instrument designed and manufactured by Metertech Inc. It
can meet the wide-ranging needs of prospective users. The main unit features fundamental photometric
functions that are ready expandable using various application software packages and attachments for
enhanced convenience and productivity. It is designed to be user-friendly and require as little engineering
knowledge as possible to adopt in users’ application.
In order to obtain maximum performance and trouble-free operation with the SP-8001, It is recommended
that you carefully read this manual before using the SP-8001, and thereafter keep it close at hand for future
reference.
§1-1 Specifications
Wavelength 190-1100nm
Bandwidth <2nm
Wavelength accuracy +/-0.5nm
Monochromator Split Beam (Internal double beam design)
Wavelength repeatability +/-0.2nm
Photometric range -0.3000-4.5000A
0.01-300 %T
0.0000-44995C
Photometric accuracy +/-0.005Abs at 1.000Abs by NIST SRM930e
Stability drifting <0.0003Abs/hr at 500nm after 1hour warm-up
Baseline flatness +/-0.001Abs (at 210-1000nm)
Stray light <0.05% at 340nm &220nm by NIST SRM2031
Light source deuterium lamp (UV range) &halogen lamp (Vis range)
Source lamp change automatic
Detector Silicon photodiodes
Wavelength scanning speed 100-3500nm/min (user-programmable)
Path-length variable at 10-100 mm
Display backlight LCD –adjustable contrast
Interface serial &parallel
Power/fuse AC input: 100/120/220/240V 50/60Hz,
Fuse: T2A for 220/240V, T4A for 100/120V
Dimension 506(W)X430(D)X220(H)mm
Weight 18kgs
Ambient temp/humidity room temperature 15-35℃,humidity <80%
Specifications subject to change without notice.
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§1-2 Safety Notes
Although this SP-8001 UV-VIS Spectrophotometer is user friendly, the instrument and its accessories
described in this manual are designed to be used by properly trained persons only. READ THIS SECTION
CAREFULLY BEFORE INSTALLING AND USING THE INSTRUMENT AND ITS ACCESSORIES.
Adjustment, maintenance, and repair of equipment must be carried out only by qualified persons who
are aware of possible hazards involved. Wherever indicated in this manual, certain maintenance may
be carried out by users, who must be fully understand and apply the following safety precautions.
For the correct and safe use of this instrument and its accessories it is essential that both operating and
servicing persons follow generally accepted safety procedures in addition to the safety precautions
specified in this manual.
Some of the chemicals used in spectrophotometer are corrosive, and/or flammable and samples may be
radioactive, toxic or potentially infective. Care should be taken to follow the normal laboratory
procedures for handling chemicals.
Only the persons who have been trained to avoid the risk of electric shock are qualified to remove the
instrument covers and accessories. The mains electricity supply to the instrument must be
disconnected at the mains supply connector and at least three minutes allowed for capacitors to
discharge.
The UV radiation from a deuterium lamp can be harmful to the skin and eyes. Always view the lamp
through protective glasses that will absorb the UV radiation and avoid looking directly at the deuterium
arc. Do not expose the skin to direct or reflected UV radiation.
Whenever it is likely that safety protection has been impaired, the instrument and/or accessories must
be made inoperative and secured against any unintended operation. The matter should then be
referred to appropriate service authority.
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Chapter 2 Installation
BEFORE installation please read safety notes in previous page carefully. Failing to abide by the regulations
may cause damages to the instrument and may obtain incorrect results.
§2-1 Unpacking
The SP-8001 (Figure 2.1 and Figure 2.2) should be delivered with intact packaging that contains one carton
with items listed in the packing list. Unpack the carton and inspect carefully. If any part is damaged or
missing, contact your local dealer immediately.
lamp compartment sample compartment
Figure 2.1 Top view of the SP-8001
Figure 2.2 Back panel of the SP-8001
Save the carton with packing materials in a proper place in case that the instrument may need to be
repacked or to be returned for service.
6
110/220V slide
power switch
fan
power receptacle
printer port
RS-232
port
LCD contrast adjuster

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§2-2 Installation
Before the first installation, please inspect carefully whether any damages or suspicious defects you may be
aware of. Do the followings before turn on the instrument. Contact your local agent for help if
encountering any difficulties during installation and operation.
A. Always place the instrument on a stable and vibration-free table. Allow two inches behind the rear
panel of the instrument for air circulation and power cord, and protect the instrument from being
located:
◆in direct sunlight
◆in drought from window, air conditioner or heater
◆near copy machine
◆near refrigerator or oven
◆near ultrasonic cleaner
◆near X-ray equipment
◆near centrifuge
B. Check and set the voltage. The SP-8001 can be performed with selective power supply at 110 or
220 Vac 50/60Hz from a properly wired and grounded single phase power outlet. Check the voltage shown
at 110/220V slide above power receptacle on rear panel. If it is not the desired, use flat screw driver to move
the slide left or right to obtain the correct voltage.
C. The room temperature of operating site should be between 15 -30℃,and humidity less than 80%.
D. Install all desired accessories and connect necessary wires and tubes properly.
E. Plug the power cord into the instrument inlet and to an appropriate wall outlet.
F. Now the system is ready for performance. Turn power on and refer details of operation to specific
sections.
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Chapter 3 General
The SP-8001 is designed to be a self-contained instrument and can work as a standalone machine or can
work with other peripherals together.
The SP-8001 has one membrane-type keyboard and a 320X240 LCD display as the user interface. User
may key in data through the keyboard and proper messages will show on the display. Alamp compartment
facilitates a tungsten halogen lamp and a deuterium lamp that users are allowed to replace those lamps
whenever needed. READ SAFETY NOTES in section §1-2 carefully and instructions in section §11-2 before
changing lamps. A sample compartment is the area for users manipulating their samples. Also it allows
users to install proper accessories for specific operations.
§3-1 User Interface
The SP-8001 keyboard with LCD display module is shown in Figure 3.1. Operations of those keys are
designed to be user friendly. In most of cases, users press keys according to instructions shown in the
corresponding screens. Otherwise, refer to specific sections of different operation modes for more details.
Figure 3.1 Keyboard with LCD display module
For operations with optional accessories, refer to proper sections in Chapter 10.
General explanation of keys is given below:
Function keys Selecting and performing supporting functions shown in the screens.
Numerical keys For entering numbers, minus, and decimal point
For entering desired wavelength.
CLEAR To clean up unwanted input digits in the entry field.
ESC To quit the current screen and go back to the previous screen, or to quit from the
current process, or to go back to upper level of function key screen.
QUICK RUN To pop up a Parameter File List (Figure 3.2) for users to select operation settings
8

AUTO ZERO To perform blank correction when needed. It sets the photometric value to 0ABS
Figure 3.2 Quick Run Parameter File List screen
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stored in memory. In this file list, users can view all parameter settings in
different operation modes that is distinguished by the first 2 letters in the first
column. For example, PH represents for “Photometric” mode. Filenames are
shown in the second column, while the corresponding time in the third column.
READ To start measuring process according to the current settings.
(100%T) after AUTO ZERO being pressed.
ENTER To confirm selections or entries of fields or parameters.
Arrow keys To move the cursor between entry fields or parameters.
Left/right arrow keys are also used for switching Yes/No as instructed.
CELL ∧To move sample holder one position up as needed.
CELL To move sample holder one position down as needed.
9
12:00:00 QUICK RUN 500.0nm 0.000A
Parameter file list
--------------------------------------
No Name Time
PH-01 ART01 07/01/99 10:12:32
PH-02
PH-03
PH-04
SP-05 HOLMIUM 07/12/99 09:30:12
SP-06
SP-07
SP-08
TS-09 LIGHT01 06/24/99 13:22:43
TS-10 LIGHT02 06/25/99 15:32:33
TS-11
TS-12
KI-13 GOT01 03/21/99 16:37:21
KI-14
KI-15
KI-16
Press UP DOWN key to select...

Save Param For saving acurrent settings into memory. Filename is entered
Figure 3.3 Parameter file list in photometric mode
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§3-2 General Function Description
Functions being used in the SP-8001 are listed in the corresponding modes and are shown in structured
maps. For those functions being widely used in most of the modes will be described below.
Load Param For loading existing parameter settings stored in memory. When this
function is pressed, aparameter file list screen (Figure 3.3) shows up
and users can choose from the list by using arrow keys and followed
by an ENTER key. Only the mode-relevant parameter setting files
will appear when function Load Param is pressed.
through a 4 by 9 array-type table (Figure 3.4). By using up/down
arrow keys for choosing rows and then numerical keys for choosing
column, the desired alphabet or number will be picked up. 10
alpha-numerical keys are the maximum for a filename. Using left
arrow key to delete the input. An ENTER key will close the input
procedure and the Parameter File List screen will show up. Using
up/down arrow keys to choose a place for the filename and followed
by an ENTER key will complete the Save Param procedure. A time
tag will be automatically attached to the saved filename. Press
function Load Param and you may see what has just been done.
Print Data To print data or graph from the current screen.
Sample Control To be functioning only when certain accessory being used.
10
12:00:00 PHOTOMETRIC 500.0nm 0.000A
Parameter file list
--------------------------------------
No Name Time
01 ART01 07/01/99 10:12:32
02
03
04
05
06
07
08
09
10
11
12
13
14
15
Press UP DOWN key to select...

Figure 3.4 Filename input screen
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Next Screen Accept the current settings and switch into the measuring display
screen. Once get the sample(s) ready, press READ for proceeding
the measurement according to the setup. Sometimes, it is used for
tracing back to the previous level of functions or for showing the
previous graph on the screen.
Prev Screen Leave the current display screen and switch back to aprevious display
screen.
Manipulate For further manipulation of current data either from new
measurement or from existing data files.
Clear Graph To clean up the current screen. This will result in loss of data in
previous measurement. Only those data for the most current
measurement can be rebuilt with function Original under function
Manipulate.
Load Data To load data with determined parameter setup from the associated
Data File List.
Save Data To save the data of current measurement or to save the data of
chosen point in function Show Track. This will also save the settings
of parameters in that measurement. Message “No data present …”
will show if there is no data in the screen.
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12:00:00 500.0nm 0.000A
1 2 3 4 5 6 7 8 9
>>>
File Name:ART
Press UP DOWN key to select...
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
0
1
2
3
4
5
6
7
8
9

Figure 3.6 System Main Menu screen
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§3-3 Initialization
Turn on the power of the SP-8001, a start-up screen (Figure 3.5) shows up and the initialization of system
will be automatically done. The version of software coming up with the instrument is also shown in the
start-up screen. User-installed accessory will be automatically detected during the initialization.
12:00:00 0.0nm 0.000A
Metertech
Metertech Inc. SP-8001
UV/VIS SPECTROPHOTOMETER
All rights reserved.
Version 1.3
Detect Accessory...Single-Cell
Initial System.....
Figure 3.5 Start-up screen
Following the initialization, the System Main Menu screen will show up (Figure 3.6). Six items representing
six operation modes are available for users to select as required. Users may use numerical keys or arrow
keys, followed by an ENTER key, to complete the selection of desired mode.
Two functions, Quick Run and Sample Control, are available in this screen. Quick Run is a function the
12
12:00:00 500.0nm 0.000A
System Main Menu
1.Photometric
2.Spectrum
3.Timescan
4.Kinetic
5.Quantitative
6.System Setup
7.Applications
Select Number:1
Quick
Run
Sample
Control

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same as described before by using the QUICKRUN key. Sample Control will be functioning only when the
multi-cell holder or the sipper is used.
13

Figure 4.1 System Setup Menu screen
Figure 4.2 Clock Parameter Setup screen
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Chapter 4 System setup
In System Main Menu, selecting item 6 will get into the System Setup Menu screen (Figure 4.1). In this
screen, users are allowed to alter several parameters described below. In this screen only one function,
Load B-Line, is available.
Load B-Line Will reload the results of Base-line measurement.
§4-1 System Setup Menu
Setup Clock To set values (Figure 4.2) for Month, Date, Year, Hour, Minute, and
14
12:00:00 500.0nm 0.000A
System Setup Menu
1.Setup Clock
2.Setup Lamp
3.Setup Printer
4.Setup RS-232
5.Setup Hardware
6.Measure Bandwidth
7.Measure Base-line
8.Search Zero Position
Select Number:1
Load
B-Line
12:00:00 500.0nm 0.000A
Clock Parameter Setup
1.Month :08
2.Date :04
3.Year :99
4.Hour :15
5.Minute :28
6.Second :00
Number from 01 to 12...
Print
Data
Prev
Screen

Figure 4.3 Lamp Parameter Setup screen
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Second as necessary.
Setup Lamp Use function keys to set up parameters for visible and UV lamps
(Figure 4.3). Switch UV is for switching ON/OFF of UV lamp. Reset
Vis Hr is for resetting the usage time of the tungsten lamp. Reset UV
Hr is for resetting the usage time of the UV lamp. Energy Vis is for
measuring the power of the tungsten lamp. Energy UV is for
measuring the power of the UV lamp.
Setup Printer Four printers (SII DPU-41X, Epson 9/24 Pin, Epson Stylus and HP
Deskjet/Laserjet) can be selected through arrow keys as instructed
(Figure 4.4).
15
12:00:00 500.0nm 0.000A
Lamp Parameter Setup
1.Visible lamp status :ON
2.Visible lamp used hours :20
3.Visible lamp energy :100%
4.UV lamp status :ON
5.UV lamp used hours :20
6.UV lamp energy :100%
Switch
UV
Reset
Vis Hr
Reset
UV Hr
Energy
Vis
Energy
UV

Figure 4.5 Serial Port Parameter Setup screen
Figure 4.4 Printer Parameter Setup screen
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Setup RS-232 Parameters for RS-232 can be set up through instructions shown in
the screen (Figure 4.5).
16
12:00:00 500.0nm 0.000A
Printer Parameter Setup
1.Printer Type :EPSON STYLUS
Available printer:
1. SII DPU-411(or DPU-414)
2. Epson 9/24 Pin series
3. Epson Stylus series
4. HP Deskjet/Laserjet series
Press < > key to select...
Print
Data
Prev
Screen
12:00:00 500.0nm 0.000A
Serial Port Parameter Setup
1.Baud rate :38400
2.Bit length :8
3.Parity check :NONE
4.Stop bit :1
5.Remote status :OFF
Press < > key to select...
Print
Data
Prev
Screen

Figure 4.6 Accessory Parameter Setup screen
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Setup Hardware In this option, three adjustable items are shown (Figure 4.6). Use
arrow keys to move cursor to proper position and proceed as
instructed at the bottom of this screen. UV/VIS Change Wavelength
allows users to set switching point for UV/VIS lamps, while 363nm is
set as default. Switch to Single Cell allows users to switch
accessories between Single Cell and accessories; e.g., Multi-Cell or
Sipper. Scan Base Line at Power On allows users to determine
whether to execute base-line measurement or not right after turning
on the power.
Measure Bandwidth To perform bandwidth measurement by pressing READ. This
measurement detects spectrum on UV lamp and the result is used for
calibration (Figure 4.7). If the calibration for this instrument is
correct, there is a peak shown around wavelength 656.1nm and the
bandwidth is less than 2nm. After calibration, two functions are
available for users to choose: Print Data for printing the results, and
Prev Screen for going back to the System Setup Menu screen.
17
12:00:00 500.0nm 0.000A
Accessory Parameter Setup
1.UV/VIS change wavelength :363.0
2.Switch to Single Cell :No
3.Scan base line at POWER ON :No
Number from 330.0 to 365.0...
Print
Data
Prev
Screen

Figure 4.7 Bandwidth measurement screen
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Measure Base line To execute the base-line measurement immediately after the ENTER
key is pressed.
Search Zero Position To perform Zero Position test for hardware.
18
12:00:00 BANDWIDTH 676.0nm
100.
0
%
50.0
0.0
636 656 676 nm
1=655.59nm
=657.44nm
Bandwidth=1.85nm
max=656.12nm
Measurement completed...
Print
Data
Prev
Screen

Figure 5.1 Photometric Parameter Setup screen
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Chapter 5 Photometric
This is a simple measurement mode with fixed wavelengths. According to the parameter setup, users can
repeat a measurement and a table of the measurement results will be shown. The results can be printed
with proper settings.
In System Main Menu, selecting item 1will get into this mode. Instrument and analysis parameters are set
up in the PHOTOMETRIC Parameter Setup screen (Figure 5.1). Move the cursor to required parameter by
using up/down arrow keys and change values of parameters. Once parameters have been set, press F5
Next Screen to get into the PHOTOMETRIC array-typed screen. When samples are prepared ready, press
READ to proceed the measurement. Measuring results will be shown in the screen.
Five functions are available in the first screen of this mode for users to select: Load Param, Save Param,
Print Data, Sample Control, and Next Screen.
§5-1 Photometric Parameter Setup
Input the Number of For entering the number of wavelength (1 to 5) and the value(s) of
wavelength to be used in the Photometric mode.
Measure Mode Selecting measurement results in ABS as for absorbance, in %T as for
transmittance, or in Conc as for concentration.
Cycle Number For entering the number of cycles (1 to 999) that asample is required
to be measured. One sample being measured through all desired
wavelengths is called as one cycle.
Cycle Interval (sec) For entering the time (0 to 9999) between the end of previous cycle
19
12:00:00 PHOTOMETRIC 500.0nm
Parameter Setup
1.Input the Number of :1
1 :500.0
2.Measure mode :ABS
Have unit(Yes/No)? :Yes
Unit :
Factor(default=1) :1.000
3.Cycle number :1
4.Cycle interval(sec) :0
5.Print every cycle :No
Number from 1 to 5...
Load
Param
Save
Param
Print
Data
Sample
Control
Next
Screen