MICROLIT E-BURETTE User manual

Designed and Manufactured by MICROLIT
629, Pakramau, Kursi Road, Lucknow – 226026, UP, India
Call at: [phone], [phone]
For queries: [email protected]
For feedback: [e-mail]
www.microlit.com
OPERATION MANUAL
Motor Operated Burette with 3 Calibrated Pre-set Speeds

Intended Use of the Instrument
Product Specifications
Safety Instructions
Functions and Limitations of Use
Operating Exclusions
Storage Conditions
List of Recommended Reagents
First Steps
Initializing
Setting Up
Computer Connectivity
Operating the instrument
Data Transfer Setup
Error Limits
Calibration
Other Settings
Cleaning and Product Maintenance
Troubleshooting
Return for repair
Calibration Guidelines
Warranty
Disposal
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
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1
2
3
4
5
6
13
16
18
20
23
29
33
34
36
38
39
39
40
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40

Intended Use of the Instrument
MICROLIT E-BURETTE is a state-of-the-art Motor Operated Burette
with sophisticated features and functionality. It is intended for use in
laboratories and the field for easy, safe and accurate titrations.
1. Safety Instructions
This instrument may sometimes be used for operations involving hazardous
materials and equipment. It is beyond the scope of this manual to address all
of the potential risks associated with its use in such applications.
It is the sole responsibility of the user to consult and establish appropriate
safety and health practices and determine the applicability of regulatory
limitations, prior to the usage of this instrument.
Please read the following instructions carefully.
Read and understand this Operation Manual thoroughly before
using the instrument.
Follow general and safety instructions for hazard prevention. E.g., always wear
protective clothing, protective gear for the eyes and hands.
Carefully observe the specifications provided by reagent manufacturers.
When dispensing inflammable fluids, avoid the built up of static charge.
Make sure that you do not dispense into plastic vessels and do not wipe the
instrument/equipment with a dry cloth.
Use the instrument only for dispensing liquids, with strict regard to the defined
limitations of use and operating constraints. (Refer to section 4)
Observe operating exclusions. When in doubt, contact the
manufacturer or supplier.
Always use the instrument in such a way that neither the user nor any other
person is in danger. While dispensing, the discharge tube must always
point away from you or any other person.
Only dispense into appropriate vessels and avoid splashes.
Do not press the piston when the discharge tube closure is attached.
Do not remove the discharge tube while the dispensing cylinder is being filled.
Reagents can accumulate in the cap of the discharge tube. Thus, clean it regularly.
Do not carry the mounted instrument by the cylinder sleeve or the valve block.
Breakage or loosening of the cylinder may lead to personal injury.
3.
•
•
•
•
•
•
•
•
•
•
•
Product Specifications2.
21
Performance Data
Unit Type
Volume Ranges
Volume Accuracy
Operating Temperature
Microprocessor-controlled motorized piston lift system with
recirculation valve
0.005 ml - 99.99 ml
(Max piston lift 10 ml,
automatic addition)
A= 0.2%, CV = 0.07%
One complete battery charge gives 3 hours (approx.)
of continuous operation, (Battery capacity indicated on TFT display and
charging time 6.5 hrs.)
+10 °C to +40 °C (from 50 °F to 104 °F)
In compliance with DIN EN ISO 8655-3
4” TFT screen
Power Supply
Quality Control
Touchscreen
Mini USB Cable
Available in 4 languages (English, Spanish, German and French)
PC Interface
Languages
Battery Operation
18 V, 3 A
0.01 ml – 250 ml
(Max piston lift 25 ml,
automatic addition)
A = 0.2%, CV = 0.07%
0.01 ml – 500 ml
(Max piston lift 50 ml,
automatic addition)
A= 0.2%, CV = 0.07%
10 ml 25 ml 50 ml

Functions and Limitations of Use
MICROLIT E-BURETTE is designed for dispensing liquids directly from
the reservoir bottle. It is calibrated in accordance with the guidelines of the
DIN EN ISO 8655 – 3. When the instrument is correctly used, the dispensed
liquid comes into contact with only the following chemically resistant materials:
PTFE, FEP and Borosilicate glass.
CE Marking
This sign certifies that the product meets the requirements of the EC directive
and has been tested according to the specified test methods.
Physical Limits
MICROLIT E-BURETTE is designed for titrating liquids,
observing the following physical limits:
Maintain instrument and reagent between +10 °C to +40 °C (from 50 °F to 104 °F).
Maintain reagent vapor pressure up to 600 mbar (maximum).
Aspirate slowly above 300 mbar in order to prevent the liquid from boiling.
Kinematic viscosity upto 500 mm2/s.
(dynamic viscosity [mPas] = kinematic viscosity [mm2 /s] x density [g/cm³])
4.
A.
B.
•
•
•
Use only original accessories and spare parts.
Do not attempt to make any technical alterations. Do not dismantle
the instrument any further than is described in the Operation Manual.
Always check the instrument for visual damage before use.
If there is any sign of a potential malfunction (e.g. piston difficult to move,
stuck valve or leakage), immediately stop dispensing.
Consult the 'Troubleshooting' section of this Operation Manual and contact the
manufacturer if needed. (Refer to section 18)
•
•
•
•
43
Relative air humidity between 20% and 90%.
Use fluids with density upto 2.2 g/cm³.
Operating Limitations
Chlorinated and fluorinated hydrocarbons or chemical combinations which
form deposits may make it difficult for the piston to move or jam it.
If the piston becomes too difficult to move, immediately clean the instrument.
(Refer to section 17)
While dispensing inflammable fluids, avoid the built up of static charge.
Make sure that you do not dispense into plastic vessels and do not wipe
instruments with a dry cloth.
MICROLIT E-BURETTE is designed for general laboratory applications and
complies with relevant standards, like DIN EN ISO 8655-3.
Please check the compatibility of the instrument for a specific application
(e.g. trace material analysis, food sector etc.).
Approvals for specific applications, like production and administration of food,
pharmaceuticals and cosmetics are not available.
Operating Exclusions
Do not use the instrument with:
Liquids that attack FEP, PFA and PTFE (e.g. dissolved Sodium Azide*).
Liquids that attack borosilicate glass (e.g. Hydrofluoric acid).
Hydrochloric acid > 40% and Nitric acid >70%.
Tetrahydrofuran or Oxolane.
Trifluoroacetic acid.
Explosive liquids (e.g. Carbon Disulfide).
Suspensions (e.g. of charcoal) as the solid particles may clog or
damage the instrument.
•
•
C.
•
•
•
5.
•
•
•
•
•
•
•
•

65
Liquids that attack PP (cap).
An aggressive atmosphere, e.g. HCl fumes.
* The permissible concentration of dissolved Sodium Azide is not more than 0.1%.
•
•
Storage Conditions
Store the instrument and accessories in a clean, cool and dry place.
Storage temperature from –20 °C to +50 °C (from –4 °F to 122 °F)
is advised with relative humidity between 5% and 95%.
6.
List of Recommended Reagents
Chemicals from A to Z
The following tables enlist the most frequently used reagents.
They provide useful information for the safe and efficient use of
MICROLIT E-BURETTE. All the safety precautions and recommendations
within this Operation Manual must be followed carefully.
Code explanations
A = Good resistance
B = Acceptable with limitations
C = Not recommended
1 = Acid vapours (better resistance with lower concentration).
Do not leave the instrument on the bottle.
Rinse with distilled water after use.
2 = Risk of damage, softening or discoloration of external parts through vapours.
Do not leave the instrument on the bottle.
Rinse with distilled water after use.
3 = Chemical degradation of glass parts (plunger/barrel)
7.

87
List of Reagents
Butyric acid
A
A
B/2
B/2
B/2
A
B/2
B/2
A
A
B/2
A
A
A
A
B/2
B/2
A
A
A
A
A
B/2
A
A
B/2
A
A
B/2
A
B/2
A
B/2
B/2
A
C/2
B/2
A
A
A
B/2
B/2
B/2
B/2
A
A
A
A
List of Reagents
Dimethylacetamide
Covi-Ox-T70/ Mixed Tocopherol
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/1/2
B/1/2
C/1/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
(Trichloromethane)

109
List of Reagents
Methyl ethyl ketone (MEK/Butanone)
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/1
C/3
C/2
C/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
List of Reagents
Oleum (Fuming Sulfuric acid)
Potassium dihydrogen phosphate
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2Picric acid
C/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A

12
Cap
Window
4” TFT Screen
Nozzle Cover
Delivery Tube
Delivery Nozzle
Middle Housing
Adapter
Retainer Ring
Telescopic Tube
Recirculation Valve
Reservoir BottleBeakerPanel
Stylus
Chuck Nut
11
List of Reagents
P
B/2
Triethylamine
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
Sulfuric acid <10%
Sulfuric acid (10-75%)
Sulfuric acid (Cold conc.)
Sulfuric acid (Hot conc.)
A
B/1
A
B/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A

13
First Steps
First and foremost – check whether everything is there in the package.
Verify that the package includes:
Operation Manual
10, 25 or 50 ml MICROLIT E-BURETTE
(Fig. 8.1)
Control Panel
(Fig. 8.2)
Telescopic Tube
(Fig. 8.3)
8.
1.
2.
3.
4.
Fig. 8.1Fig. 8.2Fig. 8.3
14
Bottle Adapters (28, 32, 38, 40, 45 mm)
(Fig. 8.4)
USB Drive for software & driver
(Fig. 8.7)
Charging AC Adaptor
(Fig. 8.5)
Control Panel Stylus
(Fig. 8.6)
5.
6.
7.
8.
Fig. 8.4Fig. 8.5Fig. 8.6Fig. 8.7

15
Mini USB Cable
(Fig. 8.8)
14.4 V, 2000 mAh Li-ion Rechargeable Batteries
(Fig. 8.11)
Extra Amber Coloured Windows
(Fig. 8.9)
Calibration Certificate
(Fig. 8.10)
9.
10.
11.
12.
Fig. 8.8Fig. 8.9Fig. 8.10Fig. 8.11
Initializing
Wear protective clothing, protective gear for the eyes and hands.
Follow all safety instructions and observe limitations of use and
operating constraints. (Refer to section 4 and 5)
Connecting the batteries
Open the battery panel at the back
of the control panel.
(Fig. 9.1)
Connect the batteries to the panel wire by inserting
the battery connector into the panel connector firmly.
It locks with a ‘click’ sound.
(Fig. 9.2)
Close the panel
(Fig. 9.3)
9.
1.
2.
3.
Fig. 9.1Fig. 9.2Fig. 9.3
16

4.
5.
Fig. 9.4Fig. 9.5
NOTE:
In order to replace the batteries, press the lock button to disengage the battery
lock and remove the old batteries.
Press the ON/OFF button.
(Fig. 9.4)
The screen will display ‘Welcome’.
(Fig. 9.5)
17
Setting Up
Adjust the length of the Telescopic tube (FEP tube) to
fit your particular reservoir.
If you require a longer tube, it is provided on request.
(Fig. 10.1)
Fix the Telescopic tube as shown. The tube’s ends
have different diameters – fix it from the wider end.
(Fig. 10.2)
Choose the correct adapter for the bottle.
The threaded platform base of MICROLIT E-BURETTE
has a 30 mm screw thread. Five adapters are supplied
to suit containers with a 28, 32, 38, 40, 45 mm and
30 mm (inbuilt adapter) screw neck.
(Fig. 10.3)
10.
1.
2.
3.
4.
Fig. 10.1Fig. 10.2Fig. 10.3
Fix the adapter on the reservoir bottle
by screwing it in clockwise direction.
(Fig. 10.4)
Fig. 10.4
18

Transporting the instrument
When mounted to a reagent bottle, always carry
the instrument as shown and always store it in the
upright position.
(Fig. 10.9)
Fig. 10.9
20
Computer Connectivity
Insert the pen drive provided with the package in your PC/Laptop for
driver files and software.
Please note that there are two set up files in the pen drive.
CDM21228
E-Burette Setup Files
Procedure to install the driver & software:
Installing the CDM21228 driver files:
11.
•
•
1.
I.
II.
Fig. 11.2 Fig. 11.1
Double click the CDM21228 setup icon.
(Fig. 11.1)
Press the Extract tab from the
FTDI CDM driver window.
(Fig. 11.2)
Mount the instrument. Screw it to the reservoir by applying gentle hand torque
applied to the threaded platform base only. In case of removal, apply the same
technique to the base, in the opposite direction.
5.
6.
7.
8.
9.
Connect the cable from the instrument housing to the
port at the back of the control panel with a firm push,
as shown. Check for the cable position.
The flat surface of the connector with an arrow mark
will face down.
(Fig. 10.5)
Fig. 10.5
If charging is required, insert the charging pin at the
back of the control panel and connect the
power cord with the AC adaptor firmly and
then plug the two pin power cord to the
220 V power supply.
(Fig. 10.6)
Fig. 10.6
The battery indicator will show the charging symbol.
(Fig. 10.7)
Fig. 10.7
The E-Burette is now ready to be switched on.
Press the ON button (of the ON/OFF button) at the
back of the control panel to switch on the control panel.
(Fig. 10.8)
Fig. 10.8
19

21
Installing the instrument software
Click the Next button for installing the device driver.
(Fig. 11.3)
Click to accept the licence agreement.
(Fig. 11.4)
Then, click the Finish button on the
device driver installation wizard.
(Fig. 11.5)
Double click the E-BURETTE setup icon.
(Fig. 11.6)
III.
IV.
V.
2.
I.
Fig. 11.4Fig. 11.5Fig. 11.6 Fig. 11.3
22
Click Next to start the Install Shield Wizard for
MICROLIT Communication window.
(Fig. 11.7)
Click Next to choose the destination
folder of the software files.
(Fig. 11.8)
Click Install for the ready to install program window.
(Fig. 11.9)
Click Next for the user account control window.
(Fig. 11.10)
II.
III.
IV.
V
Fig. 11.8Fig. 11.9Fig. 11.10 Fig. 11.7

23
Click Finish for the Install Shield Wizard
completion window.
(Fig. 11.11)
MICROLIT Communication icon will
then appear on the desktop.
(Fig. 11.12)
When it boots, the instrument will warn the
user to switch the knob to recirculation mode
(Fig. 12.1)
VI.
VII.
Fig. 11.12Fig. 12.1 Fig. 11.11
Operating the instrument
Switching on the Control Panel
Switch on the instrument using the ON/OFF switch
at the back of the control panel.
12.
1.
2.
24
Fig. 12.2Fig. 12.3Fig. 12.4
The instrument will reset automatically. It is now ready to use.
Purging
This step is recommended before using the Burette to
ensure bubble free dispensing.
3.
4.
1.
2.
Turn the knob to re-circulation mode and click the
tick mark ( ) on the control panel screen when ready.
(Fig. 12.2)
The screen on the control panel will warn the
user to turn the knob to re-circulation mode.
(Fig. 12.4)
Click on the purging mode from the
Home Screen on the control panel.
(Fig. 12.3)

25
3.
4.
1.
2.
Fig. 12.5Fig. 12.6Fig. 12.7
The E-BURETTE will purge automatically. If the device is still not properly
purged, go to step 1 and repeat the procedure till no large air bubble is
visible below the piston.
A few air bubbles up to 1 mm in size are permissible.
Titration
Wear protective clothing, protective gear for the eyes and hands. Liquid may
accumulate in the cap. Follow all safety instructions and observe limitations of
use and operating constraints. (Refer to section 4 and 5)
Turn the knob to re-circulation mode and click the
tick mark ( ) on the control panel screen when ready.
(Fig. 12.5)
Remove cap from the discharge tube.
(Fig. 12.6)
Click on the Titration mode on the home screen.
(Fig. 12.7)
26
Fig. 12.8Fig. 12.9Fig. 12.10
The screen on the control panel will warn the user to turn the knob
to titration mode.
Place the discharge tube orifice against the inner wall of a suitable receiving vessel.
Three dispensing speeds are provided, including drop-wise dispensing,
which allows the user to achieve the end point very accurately.
3.
4.
5.
6.
7.
I.
Turn the knob clockwise to set at titration mode and
click the tick ( ) mark on the screen of the control
panel when ready.
(Fig. 12.8)
Fast mode
In this mode, the dispensing speed is 2.2 ml/sec
(Fig. 12.10)
Now fill the barrel from the titration screen by clicking
the FILL button (Fig. 12.9 - left part).
By pressing the STOP button, the filling volume can be
stopped as and when you need to. (Fig. 12.9 - right part)

27
II.
III.
Fig. 12.13 Fig. 12.11Fig. 12.12
Dispensing can be performed by touching the screen and stops immediately
when the touch is removed.
8.
9.
Medium mode
In this mode, the dispensing speed is 0.75 ml/sec
(Fig. 12.11)
Dropwise mode
In this mode, the dispensing speed is 0.025 ml/sec
(Fig. 12.12)
Press the TARE button to reset to Zero & repeat.
(Fig. 12.13)
28
Fig. 12.14Fig. 12.15
Sensitive Media
For light-sensitive media (e.g., Iodine, Potassium Permanganate and
Silver Nitrate solutions), we recommend the use of ‘Amber Coloured Windows’
or the inspection windows provided in the box.
1.
2.
To replace the inspection window, unclip the default
inspection window by pressing it from its top and
then removing it.
(Fig. 12.14)
Place the Amber Coloured Window in the slot provided
at the bottom of the housing and press it down until you
hear a click sound.
(Fig. 12.15)

Data Transfer Setup
Connecting the USB Mini Cable
Software Initialization
13.
1.
2.
1.
29
Fig. 13.1Fig. 13.2Fig. 13.3
Connect the Mini USB cable from the control panel to
your PC/Laptop for transferring and storing the control
panel’s data on the latter.
(Fig. 13.1)
Insert the wider end of the USB cable in the USB port
of the PC/Laptop and the smaller end at the back of
the control panel.
(Fig. 13.2)
Double click on the Microlit Communication icon
to run the software.
(Fig. 13.3)
30
The readings will also be saved in the internal memory.
Please note that all fields are captured in the file.
2.
3.
4.
5.
6.
Fig. 13.4Fig. 13.5Fig. 13.6Fig. 13.7
From the Ports List button, select the port from
the drop down menu.
(Fig. 13.4)
Click on OPEN PORT.
(Fig. 13.5)
The window will capture the model number,
serial number and the volume range of the E-BURETTE.
(Fig. 13.6)
Click ADD from the control panel to save the readings
from the control panel to the PC/Laptop.
(Fig. 13.7)
Note:
The ADD button shall blink and the data shall be
transferred to the PC/Laptop.

31
Memory
Click ADD from the control panel to save the readings from the
control panel to the internal memory.
1.
2.
3.
Fig. 13.8 AFig. 13.8 BFig. 13.9
To recall the previous recorded reading (s),
press the RECALL button.
(Fig. 13.8 A)
Please note that the recall grid displays the last
20 recorded readings. The LAST saved reading
will be displayed on the number 1 position
of the table.
(Fig. 13.8 B)
For quick recall of the last reading,
press the LAST button.
(Fig. 13.9)
Please note that the last saved readings will be
displayed till the time LAST button is pressed.
32
Creating a File Name
Printing the data
Refresh
Fig. 13.10Fig. 13.11Fig. 13.12
The data recorded while operating the E-BURETTE
can be printed directly from the software by clicking
the PRINT tab.
(Fig. 13.11)
For this, select the printer and print the readings.
You can also access the saved .xls file to print.
To start afresh for new readings on the software,
click on the REFRESH tab.
(Fig. 13.12)
The readings on the grid shall be deleted and
new readings can now be transferred in it.
1. Select the port from the port list dropdown menu.
2. Click Open port.
3. The Software captures the Model No., Sr. no. and
volume range.
4. After transferring the data from control panel to the
software window we can save the file by clicking the
“Save as Excel File”.

Click the settings icon on the bottom
right of the Home Screen.
(Fig. 15.1)
From the settings menu, choose Calibration.
(Fig. 15.2)
Fill the nominal volume of double distilled
de-ionized water by clicking the FILL button
on the calibration screen of the operating panel.
(Fig. 15.3)
34
Calibration
User Calibration Procedure
The E-BURETTE has been laboratory calibrated at its nominal volume.
However, due to changes in environmental conditions and the viscosity
of the media which is dispensed, we recommend gravimetric testing
every 3-12 months. Gravimetric volume testing according to
DIN EN ISO 8655-6 is performed as follows:
15.
1.
2.
3.
Fig. 15.1Fig. 15.2Fig. 15.3
33
Exit
Click on the tick ( ) button to proceed. The piston will empty the barrel and
go to the home position.
Press the ON/OFF button to switch OFF the control panel.
1.
2.
3.
Fig. 13.13
To end working with the E-BURETTE,
press the Exit button
(Fig. 13.13).
The panel will warn the user to turn the
knob in the re-circulation mode.
Error Limits
The error limits (Accuracy and Coefficient of Variation) mentioned in
Product Specifications (Page 1) are in accordance with the nominal capacity
(or maximum volume) indicated on the instrument.
These are obtained by using the instrument with distilled water at
equilibrium, ambient temperature of 20 °C, while operating the device
smoothly and steadily.
The error limits are well within the limits of DIN EN ISO 8655-3.
14.
10
Capacity (ml) Increment Accuracy
±% ±ml
0.2
CV
±% ±ml
25
50
0.005 ml
0.01 ml
0.01 ml
0.2 0.07 0.007
0.2 0.05 0.07 0.0175
0.2 0.1 0.07 0.035

Click on the settings icon from the home screen.
(Fig. 16.1)
In the settings windows, click the BRIGHTNESS icon.
(Fig. 16.2)
From the BRIGHTNESS window, select the level of
brightness and tick mark to save it.
(Fig. 16.3)
Other Settings
Control panel brightness setting
User can change brightness from setting screen on the control panel.
16.
1.
2.
3.
Fig. 16.1Fig. 16.2Fig. 16.3
3635
Dispense the filled liquid by clicking on the
DISPENSE button on the calibration screen
of the control panel.
(Fig. 15.4)
The factory reset is for the MICROLIT factory settings
which is password protected and intended to be used
at the manufacturing facility. (Fig. 15.5)
If the reset button is pressed, the user calibrated settings
shall be reset with the default factory settings & the CAL
label will disappear from the screens.
Measure the dispensed liquid on a balance and enter the value in mg using
the provided electronic keyboard. Click the tick mark ( ) to proceed.
Note: CAL label will start appearing on top of all screens.
Repeat this procedure until the nominal volume is achieved
on the electronic balance.
Factory Reset
4.
5.
6.
Fig. 15.4Fig. 15.5