MICROLIT BEATUS User manual

Designed and Manufactured by MICROLIT
629, Pakramau, Kursi Road, Lucknow – 226026, UP, India
Call at: [phone], [phone]
For queries: [email protected]
For feedback: [e-mail]
www.microlit.com
OPERATION MANUAL
Bottle Top Dispenser with Recirculation Valve and Springless Valve™ Technology

Intended Use of the Instrument
Safety Instructions
Functions and Limitations of Use
Operating Exclusions
Storage Conditions
List of Recommended Reagents
First Steps
Setting Up
Priming
Volume Setting and Dispensing
Error Limits
User Calibration Procedure
Maintenance / Cleaning
Disassembling and Assembling for
Cleaning and Servicing
Autoclaving
Troubleshooting
Guidelines for Return
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
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1
2
3
3
4
11
13
15
16
18
19
20
21
24
27
28

Intended Use of the Instrument
MICROLIT BEATUS is a Bottle Top Dispenser (BTD). It is a high precision
liquid handling instrument intended for use in laboratories for dispensing
reagents and chemicals that are compatible with it. (Refer to section 6)
1.
Safety Instructions
This instrument may sometimes be used for operations involving hazardous
materials and equipment. It is beyond the scope of this manual to address all
of the potential risks associated with its use in such applications.
It is the sole responsibility of the user to consult and establish appropriate safety
and health practices and determine the applicability of regulatory limitations,
prior to the usage of this instrument.
Please read the following instructions carefully.
Read and understand this Operation Manual thoroughly before
using the instrument.
Follow general and safety instructions for hazard prevention.
E.g., always wear protective clothing, protective gear for the eyes and hands.
Carefully observe the specifications provided by reagent manufacturers.
When dispensing inflammable fluids, avoid the built up of static charge.
Make sure that you do not dispense into plastic vessels and do not wipe
the instrument/equipment with a dry cloth.
Use the instrument only for dispensing liquids, with strict regard to the defined
limitations of use and operating constraints. (Refer to section 3 and 4)
Observe operating exclusions. When in doubt, contact the
manufacturer or supplier.
Always use the instrument in such a way that neither the user nor any
other person is in danger. While dispensing, the discharge tube must always
point away from you or any other person. Only dispense into appropriate
vessels and avoid splashes.
Do not press the piston when the discharge tube closure is attached.
Do not remove the discharge tube while the dispensing cylinder is being filled.
Reagents can accumulate in the cap of the discharge tube.
Thus, clean it regularly.
Do not carry the mounted instrument by the cylinder sleeve or the valve block.
Breakage or loosening of the cylinder may lead to personal injury.
Do not use the instrument with force. Apply smooth gentle movements to
operate the piston. Use only original accessories and spare parts.
Do not attempt to make any technical alterations. Do not dismantle the instrument
any further than is described in the Operation Manual.
Always check the instrument for visual damage before use.
If there is any sign of a potential malfunction (e.g. piston difficult to move,
stuck valve or leakage), immediately stop dispensing. Consult the 'Troubleshooting'
section of this Operation Manual and contact the manufacturer if needed.
(Refer to section 16)
2.
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Functions and Limitations of Use
MICROLIT BEATUS is designed to dispense liquids directly from the
reservoir bottle. It is calibrated in accordance with the guidelines of the
DIN EN ISO 8655 – 5.
When the instrument is correctly used, the dispensed liquid comes into contact
with only the following chemically resistant materials:
PTFE, FEP and Borosilicate glass.
Physical limits
MICROLIT BEATUS is designed to be operated under the following physical limits.
Maintain instrument temperature between +15°C and +40°C (from 59°F to 104°F).
Maintain reagent vapour pressure up to 600 mbar (maximum). Aspirate slowly
above 300 mbar in order to prevent the liquid from boiling.
Kinematic viscosity upto 500 mm²/s.
(dynamic viscosity [mPas] = kinematic viscosity [mm² /s] x density [g/cm³])
Use fluids with density upto 2.2 g/cm³.
3.
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21

List of Recommended Reagents
Chemicals from A to Z
The following tables enlist the most frequently used reagents.
They provide useful information for the safe and efficient use of
MICROLIT BEATUS. All the safety precautions and recommendations
within this Operation Manual must be followed carefully.
Code explanations:
A = Good resistance
B = Acceptable with limitations
C = Not recommended
1 = Acid vapours (better resistance with lower concentration).
Do not leave the instrument on the bottle.
Rinse with distilled water after use.
2 = Risk of damage, softening or discoloration of external parts through vapours.
Do not leave the instrument on the bottle.
Rinse with distilled water after use.
3 = Chemical degradation of glass parts (plunger/barrel)
6.
Operating Exclusions
Do not use the instrument with:
Liquids that attack FEP, PFA and PTFE (e.g. dissolved Sodium Azide*)
Liquids that attack borosilicate glass (e.g. Hydrofluoric acid)
Hydrochloric acid > 40% and Nitric acid >70% | Tetrahydrofuran |
Trifluoroacetic acid
Explosive liquids (e.g. Carbon Disulfide)
Suspensions (e.g. of charcoal) as the solid particles may clog or
damage the instrument
Liquids that attack PP (cap)
* The permissible concentration of dissolved Sodium Azide is not more than 0.1%.
4.
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Storage Conditions
Store the instrument and accessories in a clean, cool and dry place.
Storage temperature may vary from – 20°C to +50°C (from – 4°F to 122°F)
5.
Operating limitations
Liquids, which form deposits, may make it difficult for the piston to move or
jam it (like crystallizing solutions or concentrated alkaline solutions).
If the piston becomes too difficult to move, immediately clean the instrument.
(Refer to section 13)
While dispensing inflammable fluids, avoid the built up of static charge.
Make sure that you do not dispense into plastic vessels and do not wipe
instruments with a dry cloth.
MICROLIT BEATUS is designed for general laboratory applications and
complies with relevant standards, like DIN EN ISO 8655. Please check the
compatibility of the instrument for a specific application (e.g. trace material
analysis, food sector etc.). Approvals for specific applications, like production
and administration of food, pharmaceuticals and cosmetics are not available.
•
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43

65
List of Reagents
Butyric acid
A
A
B/2
B/2
B/2
A
B/2
B/2
A
A
B/2
A
A
A
A
B/2
B/2
A
A
A
A
A
B/2
A
A
B/2
A
A
B/2
A
B/2
A
B/2
B/2
A
C/2
B/2
A
A
A
B/2
B/2
B/2
B/2
A
A
A
A
List of Reagents
Dimethylacetamide
Covi-Ox-T70/ Mixed Tocopherol
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/1/2
B/1/2
C/1/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
(Trichloromethane)

87
List of Reagents
Methyl ethyl ketone (MEK/Butanone)
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/1
C/3
C/2
C/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
List of Reagents
Oleum (Fuming Sulfuric acid)
Potassium dihydrogen phosphate
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2Picric acid
C/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A

109
Cap
Main Housing
Middle Housing Retainer
Recirculation Value
Delivery Pipe Cover
Delivery Nozzle
Chuck Nut
Nozzle Cap
Volume Adjustment Knob
Middle Body
Barrel
Adapter
Bottle Adapter
Telescopic Tube
List of Reagents
P
B/2
Triethylamine
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
B/2
Sulfuric acid <10%
Sulfuric acid (10-75%)
Sulfuric acid (Cold conc.)
Sulfuric acid (Hot conc.)
A
B/1
A
B/2
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A

1211
First Steps
First and foremost – check whether everything is there in the package.
Verify that the package includes:
1 Operation Manual
7.
1.
2.
3.
1 Bottle Top Dispenser
(Fig. 7.1)
1 Telescopic Tube
(Fig. 7.2)
1 Calibration Tool
(Fig. 7.3)
Fig. 7.1
4.
5.
6.
5 Bottle Adapters
(28 mm, 32 mm, 38 mm, 40 mm, 45 mm)
(Fig. 7.4)
1 Calibration Certificate
(Fig. 7.5)
Fig. 7.4Fig. 7.5
Fig. 7.2
Fig. 7.3

1413
4.
5.
The instrument is all set up and ready to use.
Do not operate the piston until the entire unit is safely and fully
mounted on the reservoir bottle.
Always wear rubber gloves while operating the instrument or handling
the bottle, especially when working with hazardous liquids.
When mounted to a reagent bottle, always carry the instrument carefully.
Fig. 8.4
Fix the adapter.
(Fig. 8.4)
Fig. 8.5Fig. 8.6
Mount the instrument. Screw it to the reservoir
by applying gentle hand torque applied to the
threaded platform base only. In case of removal,
apply the same technique to the base, in the
opposite direction.
(Fig. 8.5)
Do not press the piston down when the cap is on.
Avoid splashing the reagent.
The reagent can drip out
from the discharge tube and cap.
(Fig. 8.6)
Fig. 8.1
Setting Up
Wear protective clothing, protective gear for the eyes and hands. Follow all
safety instructions and observe limitations of use and operating constraints.
(Refer to sections - 2,3,4)
8.
1.
2.
3.
Fig. 8.2
Adjust the length of the telescopic tube to
fit your particular reservoir. If you require a
longer tube, it is provided on request.
(Fig. 8.1)
Fix the telescopic tube. The tube’s ends have
different diameters. Please select the correct side
for your instrument.
(Fig. 8.2)
Fig. 8.3
Choose the correct adapter for the bottle.
The threaded platform base has a 30 mm
screw thread. Five adapters are supplied to
suit containers with a 28, 32, 38, 40, 45 mm
and 30 mm (inbuilt adapter) screw neck.
(Fig. 8.3)

1615
Fig. 9.1
Priming9.
1.
2.
3.
Fig. 9.2Fig. 9.3Fig. 9.4
First, open the cap of the dispensing tube.
For your safety, hold the discharge tube orifice against
the inner wall of the appropriate receiving vessel.
(Fig. 9.1)
Set the valve to ‘Recirculate’.
(Fig. 9.2)
Turn the valve to ‘Dispenser’.
To avoid splashes while priming, hold the discharge tube
against the inner wall of the appropriate receiving vessel.
Dispense liquid to prime the discharge tube until it is
bubble free. Wipe away any remaining drops from the
discharge tube.
Before using the instrument for the first time, ensure
that it has been rinsed carefully and discard the first few
samples dispensed. Avoid splashing.
For priming, gently pull the piston up
(approximately by 30mm) and then down rapidly,
till the lower end. (Fig. 9.3)
Repeat this procedure five times.
•
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Fig. 10.1Fig. 10.2
Volume Setting and Dispensing
Volume setting
Volume Adjustment Knob is simple and easy to operate.
There are two positions of the knob as shown:
To set the volume, follow these simple steps:
Unlock the Knob by rotating it ANTICLOCKWISE.
The slider is now loose and can be moved up and down.
Set your desired volume by aligning the pointer with the scale.
To lock the set volume, turn the Knob from Position 2 to
Position 1 by rotating it CLOCKWISE.
Over rotating the knob may lead to breakage.
10.
1.
2.
3.
4.
Position 1
Locked
(Fig. 10.1)
Position 2
Unlocked
(Fig. 10.2)

1817
Fig. 10.3Fig. 10.4Fig. 10.5
Dispensing
Wear protective clothing, protective gear for the eyes and hands.
Liquid may accumulate in the cap. To avoid splashes, dispense slowly.
Follow all safety instructions and observe limitations of use and
operating constraints.
1.
2.
3.
Error Limits
The error limits (Accuracy and Coefficient of Variation) mentioned below are
in accordance with the nominal capacity (or maximum volume) indicated on
the instrument. These are obtained by using the instrument with distilled water
at equilibrium, ambient temperature of 20 °C or 68°F, while operating the device
smoothly and steadily.
The error limits are well within the limits of DIN EN ISO 8655-5.
11.
Remove cap from the discharge tube.
(Fig. 10.3)
Before using the instrument, turn the valve
to ‘Dispensing’.
(Fig. 10.4)
Hold the discharge tube orifice against the
inner wall of the appropriate receiving vessel.
(Fig. 10.5)
Fig. 10.6Fig. 10.7
Wipe off the discharge tube against the inner wall of the receiving vessel.
4.
5.
6.
Gently lift the piston until the upper end. Press the
piston downwards slowly and steadily with minimal
force until the lower end.
(Fig. 10.6)
Reattach the cap to the discharge tube.
(Fig. 10.7)
Volume
Range
0.25-2.5 ml 0.05 ml 0.6 0.2
0.2
0.2
0.2
0.2
0.2
0.005
0.010
0.020
0.060
0.120
0.200
0.015
0.030
0.060
0.180
0.360
0.600
0.6
0.6
0.6
0.6
0.6
0.1 ml
0.2 ml
0.5 ml
1.0 ml
2.0 ml
0.5-5 ml
1-10 ml
2.5-30 ml
5-60 ml
10-100 ml
Increment
Specifications ISO 8655
Accuracy
±% ±ml
CV
±% ±ml

2019
User Calibration Procedure
MICROLIT BEATUS is laboratory calibrated at its nominal volume.
However, due to changes in environmental conditions and the viscosity
of the media / fluids which are dispensed, we recommend gravimetric
volume testing every 3-12 months.
Gravimetric volume testing, in accordance with DIN EN ISO 8655-6, is performed
as follows (for measurement conditions, see section12):
Re-calibration
Set the instrument to the nominal volume or any other volume which is most
commonly used by you. Follow the common rules for calibration used in
statistical quality control (ISO 8655-5).
Set the volume and dispense five full volumes of distilled water at 20°C on an
electronic balance to establish the actual mean volume of liquid dispensed.
If the gravitational average result varies from the volume displayed, you should
re-calibrate the instrument.
12.
1.
2.
3.
4.
Maintenance / Cleaning
MICROLIT BEATUS should be cleaned in the following situations:
Immediately when the piston is difficult to move
Before changing the reagent
Prior to long term storage
Prior to dismantling the instrument
Prior to autoclaving
Prior to changing the valve
Regularly when using liquids which form deposits (e.g. crystallizing liquids)
Regularly, when liquids accumulate in the cap.
All maintenance/cleaning procedures should be carried out while wearing
suitable eye protection and protective clothing.
When in doubt, consult your safety officer.
Make sure that the instrument is completely empty.
Place the instrument into an empty sink together with its reservoir.
Unscrew the threaded platform base from the reservoir and lift the instrument's
intake tube carefully out of the reservoir, whilst tapping it against the reservoir's
aperture to shake off any droplets from the intake tube.
Hold the dispensing nozzle over the aperture of the reservoir and apply gentle
piston strokes in order to return any contents into the reservoir.
Empty the instrument completely and flush thoroughly with distilled water.
If the piston barrel is still not completely clean, you need to disassemble
the instrument. (Refer to section 14)
13.
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Fig. 12.2
For re-calibration, pull the cap outwards to
expose the calibration nut.
(Fig. 12.1)
Using the calibration tool, turn the calibration nut
clockwise to reduce the volume and anticlockwise
to increase it.
Repeat this procedure till the desired volume is
achieved on the electronic balance.
(Fig. 12.2)
Fig. 12.1

2221
Disassembling and Assembling for Cleaning and Servicing
Procedure to disassemble the piston:
Flush all the parts of the instrument with deionized water.
14.
1.
2.
3.
4.
5.
6.
7.
8.
Fig. 14.6
Fig. 14.2 Fig. 14.1
Fig. 14.5Fig. 14.7
Fig. 14.3Fig. 14.4
Pull the cap outwards to expose the calibration nut.
(Fig. 14.1)
Unscrew the calibration nut with the help of
calibration tool to disassemble the piston and
shaft from the main housing.
(Fig. 14.2)
Clean the cylinder with a bottle-brush.
If necessary, carefully remove deposits
at the edge of the glass cylinder.
(Fig. 14.5)
Insert the piston completely into the cylinder and then
reassemble the instrument using the calibration tool
by screwing back the piston.
(Fig. 14.6)
Snap the cap back to complete the assembly.
(Fig. 14.7)
After unscrewing, pull out the shaft.
(Fig. 14.3)
Rinse the piston and shaft with deionized water.
(Fig. 14.4)

Autoclaving
MICROLIT BEATUS is fully autoclavable at 121°C (or 250°F) and
1 bar absolute (15 psi) with a holding time of at least 15 minutes.
NOTE – Only the piston needs to be removed for autoclaving
the instrument. Piston is also autoclavable.
Disassembling for autoclaving
15.
1.
2.
3.
2423
Procedure to disassemble the Delivery Pipe
Clean the pipe with deionized water.
Procedure to re-assemble the Delivery Pipe
Fig. 14.9Fig. 14.11 Fig. 14.8Fig. 14.10
Fig. 15.2Fig. 15.3
Straighten the delivery pipe.
(Fig. 14.8)
Pull the cap outwards to expose the calibration nut.
(Fig. 15.1)
Unscrew the calibration nut with the help of
calibration tool to disassemble the piston and
shaft from the main housing.
(Fig. 15.2)
After unscrewing, pull out the shaft.
(Fig. 15.3)
Unscrew the chuck nut and pull out the delivery pipe.
(Fig. 14.9)
First push the delivery pipe into the lower
housing till it stops going in further.
(Fig. 14.10)
Screw the chuck nut to complete the assembly.
(Fig. 14.11)
1.
2.
3.
1.
2.
Fig. 15.1

Fig. 15.6
2625
Dispenser is now ready for use. Recalibration is required after autoclaving.
2.
Fig. 15.7
Insert the piston completely into the cylinder and
then reassemble the instrument.
Use the calibration tool to screwing the piston.
(Fig. 15.6)
Snap the cap back to complete the assembly.
(Fig. 15.7)
Cooling
After autoclaving, cool the dispenser at room temperature for
at least 2 hours before use.
Re-assembling after autoclaving
4.
5.
1.
Fig. 15.4Fig. 15.5
This is the piston-shaft sub-assembly.
(Fig. 15.4)
Autoclave the two sub-assemblies at 121°C and
15 psi pressure for 10-15 minutes.
(Fig. 15.5)
6. The volume adjustment knob should always be kept in the 'unlocked' position while
autoclaving (Refer fig 10.2).

Troubleshooting16. Guidelines for Return
Follow these guidelines, before sending the instrument for repair:
Please clean the instrument carefully.
On no account should the sent instrument contain any reagent.
The sent instrument can only be checked and repaired if it has
been carefully cleaned and decontaminated, beforehand.
Forcefully opening the instrument invalidates any warranty claim.
While packing the instrument for return, please enclose a detailed
description of the trouble that has occurred and the reagents that
were used with the instrument.
17.
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2827
Problem Possible cause Solution
Piston difficult to move Formation of crystals or
deposition of dirt
Stop dispensing immediately.
Loosen piston in circular motion,
but do not disassemble.
Follow the cleaning instructions.
(Refer to section13)
Air bubbles appear
in the instrument
Reagent with high vapour
pressure has been drawn
in too quickly.
The instrument has not
been primed.
Filling tube is loose or damaged.
Liquid reservoir is empty.
The filling action is too fast.
The piston is leaking.
The discharge valve is leaking
Draw the reagent slowly.
Prime the instrument
(Refer to section 9)
Push the filling tube on firmly.
If necessary, cut off approx.
1 cm of the tube at the upper
end and then re-connect it
or replace it.
Refill reservoir and
prime the unit.
Fill and dispense slowly.
Clean the piston. Refer to
section 13. If problem persists,
replace it.
Clean by flushing thoroughly
with distilled water.
(Refer to section 13)
Dispensing not possible Dispensing nozzle is blocked.
Discharge valve is stuck.
Disassemble the dispensing
nozzle and flush thoroughly
with distilled water.
Clean the unit by immersing
valve assembly in distilled water.
(Refer to section 13)
Wrong dispensing volume The instrument is not calibrated. Follow user calibration steps.
(Refer to section 12)
Barrel does not fill with liquid Inlet tube is not fitted firmly. Connect inlet tube correctly.
(Refer to section 8)
Filling not possible Adjust volume to
minimum setting.
Set to the required volume.
(Refer to section 10)