Motic AE30 User manual

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We are constantly endeavouri
ng to improve our instruments and to adapt them to the requirements
of modern research techniques and testing methods. This involves modification to the mechanical
structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are
subject to change without notice.

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TABLE OF CONTENTS
SECTION
INomenclature ………………………………………………………………………………………… 3
II Specification ………………………………………………………………………………………… 5
III Setting-up the Instrument ………………………………………………………………………… 6
IV Assembling the microscope Input voltage ……………………………………………………… 6
1 Installing the lamp ………………………………………………………………………………… 7
2 Mounting the condenser ………………………………………………………………………… 7
3 Installing the Objectives ………………………………………………………………………… 8
4 Mechanical Stage ………………………………………………………………………………… 8
5 Mounting the eyepieces ………………………………………………………………………… 8
VMicroscopic procedure Interpupillary disatance adjustment ……………………………… 9
1 Diopter adjustment ………………………………………………………………………………… 9
2 Centering the condenser ………………………………………………………………………… 10
3 Centering the lamp ………………………………………………………………………………… 10
4 Brightfield microscopy …………………………………………………………………………… 11
5 Phase-contrast microscopy …………………………………………………………………… 12
VI Photomicrographic procedure …………………………………………………………………… 13
VII Troubleshooting Table ……………………………………………………………………………… 14
VIII Care and maintenance ……………………………………………………………………………… 15
1 Lenses and filters ………………………………………………………………………………… 15
2 Cleaning of painted or plastic components…………………………………………………… 15
3 When not in use …………………………………………………………………………………… 15
IX Warning Lable ………………………………………………………………………………………… 15

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INomenclature
1. Lamp Socket Clamp Screw Knob
2. Lamp House Cover Clamp Screw
3. Annular Diaphragm
4. Condenser Diaphragm Lever
5. Condenser Focus Knob
6. Condenser Lens
7. Stage Plate Insert
8.Objectives
9. Revolving Nosepiece
10. Coaxial Coarse/Fine Focus Knob
11. Torque Adjusting Ring
12. Filter Retaining Ring
13. Hexagonal Centering Screwdrivers (x2)

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1. Vertical Photo Port
2. Interpupillary Distance Scale
3. Diopter Adjustment Ring
4. Eyepiece
5. Optical Path Selector Lever
6. Filter Slider
7. Lamp House
8. Field Diaphragm Lever
9. Condenser Clamp Screw
10. Phase Slider
11. Condenser Clamp Holder Screw
12. Condenser Centering Screws
13. Stage Plate
14. Fluorescence Filter cassette Mount
15. Power Switch
16. Light Intensity Control Dial
17. Brightness Indicator
(LED Segmented Display)

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II Specifications
•Magnification Ratio : 40X-600X
•Eyepiece : Objective field φ22
•Objectives :
Maganification N.A. W.D.
4X 0.1 23.5
10X 0.25 7.5
20X 0.4 7
40X 0.6 2.8
60X (Optional) 0.7 1.4
•Condenser : 1. N.A. 0.3 / W.D. 72mm
2. N.A. 0.5 / W.D. 28mm (Optional)
•LED : 6V / 30W Halogen Illumination Adjustable
•Electrical Specifications:
Input : 90-240VAC, 35W, 50-60HZ
Output : 12V, 30W
Fuse : T2.5AL250V (If the orginal fuese is damaged, please replace a new one with same specification.

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III Setting-up the Instrument
Working environment
•The location should be free from dust, moisture, chemical vapours and mechanical vibrations.
•Do not situate the instrument in a warm and/or humid environment.
•Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp
or a well-lit.
bright wall. The quality of the viewed image from the microscope will deteriorate where there is
significant ambient light.
Operating environment
•Indoor use.
•Altitude:Max 2000 meters
•Ambient temperature: 15°C to 35°C
•Maximum relative humidity: Relative Humidity of not more than 75%
•Supply voltage fluctuations: Not to exceed ±10% of the normal voltage.
•Pollution degree: 2(in according with IEC60664)
•Installation/Overvoltage category:(inaccording withIEC606Ⅱ64)
•Air Pressure of 75kPa to 106 kPa
•No hoar frost, dew, percolating water, rain
IV Assembling the microscope Input voltage
•Automatic voltage selection works with electrical outlets worldwide. However, always use a power
cord that is rated for the voltage used in your area and that has been approved to meet local safety
standards. Using the wrong power cord could cause fire or equipment damage.
•In case of using the extension cord, use only the power supply cord with the PE (protective earth)
wire.
•In order to prevent electric shock, always turn the power switch on the power supply off before
connecting the power cord.
Power Cord

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1Installing the lamp
•In order to prevent electric shock always turn the power switch off and unplug the power cord
before replacing the lamp.
•Release lamp housing cover clamp screw using a coin to remove the cover.
•Firmly insert the lamp into the socket pinholes until it reaches the limit, be careful not to tilt the
lamp when mounting.
•When installing the lamp, do not touch the glass surface of the lamp with bare fingers. Doing so
will cause fingerprints , grease, etc., to burn onto the lamp surface, reducing the illumination
provided by the lamp. If surface is contaminated, wipe it clean using lens tissue.
•Close the cover and fasten with it with lamp housing cover clamp screw.
•Insert the filter slider with mat surface of the diffuser turned towards the user.
2Mounting the condenser
•and index marks facing the front and secure it with the clamp screw.Mount the ELWD condenser
on the circular dovetail mount of the condenser holder with the aperture diaphragm lever
•Insert the Ph annular diaphragm slider with centering hexagonal socket head screws facing
the front.
•The centerable condenser mount is height adjustable with rack and pinion and is dovetail
mounted on the illuminating pillar with a clamp screw.
Lamp Housing Cover
Clamp Screw
Power Switch
C
ondenser Clamp
Screw

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3Installing the Objectives
•Remove the stage plate insert from the stage.
•Install the objectives into the nosepiece so that the magnification increases with clockwise rotation
of the revolving nosepiece.
•Replace the stage plate insert.
4Mechanical Stage
•Secure the mechanical stage to the AE30/31 plain stage using the two mounting screws located
beneath the stage on the right side
5Mounting the eyepieces
•Remove the dust caps from the eyepiece tubes.
•Insert the eyepieces into the eyepiece tubes.
•If the rubber eye guards are to be used, fit them in the groove around the eyepiece.

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VMicroscopic procedure Interpupillary distance adjustment
•Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
•Adjust the interpupillary distance so that both the right and left field of view become one.
•This adjustment will enable the user to observe the specimen with both eyes
.
1Diopter adjustment
•Diopter adjustment compensates for differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the
extent to which focusing is lost when the objective magnification is changed. In particular,
this occurs when a low power objective is used.
•Before adjusting the diopter, bring a specimen into focus using the 10x objective.
•Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to
“0” position.
•Position 40x objective into the optical path and bring the specimen image into focus by turning
the coarse and fine focus knobs.
•Position either 4x or 10x objective into optical path. Without adjusting the fine and coarse focus
knobs, turn the diopter rings on the eyepieces so that the specimen images in the left and right
eyepieces are focused individually.
•Repeat the above step twice.
Diopter adjustment “0”
position