Motic AE30 User manual

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We are constantly endeavouri
ng to improve our instruments and to adapt them to the requirements
of modern research techniques and testing methods. This involves modification to the mechanical
structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are
subject to change without notice.

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TABLE OF CONTENTS
SECTION
INomenclature ………………………………………………………………………………………… 3
II Specification ………………………………………………………………………………………… 5
III Setting-up the Instrument ………………………………………………………………………… 6
IV Assembling the microscope Input voltage ……………………………………………………… 6
1 Installing the lamp ………………………………………………………………………………… 7
2 Mounting the condenser ………………………………………………………………………… 7
3 Installing the Objectives ………………………………………………………………………… 8
4 Mechanical Stage ………………………………………………………………………………… 8
5 Mounting the eyepieces ………………………………………………………………………… 8
VMicroscopic procedure Interpupillary disatance adjustment ……………………………… 9
1 Diopter adjustment ………………………………………………………………………………… 9
2 Centering the condenser ………………………………………………………………………… 10
3 Centering the lamp ………………………………………………………………………………… 10
4 Brightfield microscopy …………………………………………………………………………… 11
5 Phase-contrast microscopy …………………………………………………………………… 12
VI Photomicrographic procedure …………………………………………………………………… 13
VII Troubleshooting Table ……………………………………………………………………………… 14
VIII Care and maintenance ……………………………………………………………………………… 15
1 Lenses and filters ………………………………………………………………………………… 15
2 Cleaning of painted or plastic components…………………………………………………… 15
3 When not in use …………………………………………………………………………………… 15
IX Warning Lable ………………………………………………………………………………………… 15

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INomenclature
1. Lamp Socket Clamp Screw Knob
2. Lamp House Cover Clamp Screw
3. Annular Diaphragm
4. Condenser Diaphragm Lever
5. Condenser Focus Knob
6. Condenser Lens
7. Stage Plate Insert
8.Objectives
9. Revolving Nosepiece
10. Coaxial Coarse/Fine Focus Knob
11. Torque Adjusting Ring
12. Filter Retaining Ring
13. Hexagonal Centering Screwdrivers (x2)

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1. Vertical Photo Port
2. Interpupillary Distance Scale
3. Diopter Adjustment Ring
4. Eyepiece
5. Optical Path Selector Lever
6. Filter Slider
7. Lamp House
8. Field Diaphragm Lever
9. Condenser Clamp Screw
10. Phase Slider
11. Condenser Clamp Holder Screw
12. Condenser Centering Screws
13. Stage Plate
14. Fluorescence Filter cassette Mount
15. Power Switch
16. Light Intensity Control Dial
17. Brightness Indicator
(LED Segmented Display)

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II Specifications
•Magnification Ratio : 40X-600X
•Eyepiece : Objective field φ22
•Objectives :
Maganification N.A. W.D.
4X 0.1 23.5
10X 0.25 7.5
20X 0.4 7
40X 0.6 2.8
60X (Optional) 0.7 1.4
•Condenser : 1. N.A. 0.3 / W.D. 72mm
2. N.A. 0.5 / W.D. 28mm (Optional)
•LED : 6V / 30W Halogen Illumination Adjustable
•Electrical Specifications:
Input : 90-240VAC, 35W, 50-60HZ
Output : 12V, 30W
Fuse : T2.5AL250V (If the orginal fuese is damaged, please replace a new one with same specification.

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III Setting-up the Instrument
Working environment
•The location should be free from dust, moisture, chemical vapours and mechanical vibrations.
•Do not situate the instrument in a warm and/or humid environment.
•Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp
or a well-lit.
bright wall. The quality of the viewed image from the microscope will deteriorate where there is
significant ambient light.
Operating environment
•Indoor use.
•Altitude:Max 2000 meters
•Ambient temperature: 15°C to 35°C
•Maximum relative humidity: Relative Humidity of not more than 75%
•Supply voltage fluctuations: Not to exceed ±10% of the normal voltage.
•Pollution degree: 2(in according with IEC60664)
•Installation/Overvoltage category:(inaccording withIEC606Ⅱ64)
•Air Pressure of 75kPa to 106 kPa
•No hoar frost, dew, percolating water, rain
IV Assembling the microscope Input voltage
•Automatic voltage selection works with electrical outlets worldwide. However, always use a power
cord that is rated for the voltage used in your area and that has been approved to meet local safety
standards. Using the wrong power cord could cause fire or equipment damage.
•In case of using the extension cord, use only the power supply cord with the PE (protective earth)
wire.
•In order to prevent electric shock, always turn the power switch on the power supply off before
connecting the power cord.
Power Cord

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1Installing the lamp
•In order to prevent electric shock always turn the power switch off and unplug the power cord
before replacing the lamp.
•Release lamp housing cover clamp screw using a coin to remove the cover.
•Firmly insert the lamp into the socket pinholes until it reaches the limit, be careful not to tilt the
lamp when mounting.
•When installing the lamp, do not touch the glass surface of the lamp with bare fingers. Doing so
will cause fingerprints , grease, etc., to burn onto the lamp surface, reducing the illumination
provided by the lamp. If surface is contaminated, wipe it clean using lens tissue.
•Close the cover and fasten with it with lamp housing cover clamp screw.
•Insert the filter slider with mat surface of the diffuser turned towards the user.
2Mounting the condenser
•and index marks facing the front and secure it with the clamp screw.Mount the ELWD condenser
on the circular dovetail mount of the condenser holder with the aperture diaphragm lever
•Insert the Ph annular diaphragm slider with centering hexagonal socket head screws facing
the front.
•The centerable condenser mount is height adjustable with rack and pinion and is dovetail
mounted on the illuminating pillar with a clamp screw.
Lamp Housing Cover
Clamp Screw
Power Switch
C
ondenser Clamp
Screw

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3Installing the Objectives
•Remove the stage plate insert from the stage.
•Install the objectives into the nosepiece so that the magnification increases with clockwise rotation
of the revolving nosepiece.
•Replace the stage plate insert.
4Mechanical Stage
•Secure the mechanical stage to the AE30/31 plain stage using the two mounting screws located
beneath the stage on the right side
5Mounting the eyepieces
•Remove the dust caps from the eyepiece tubes.
•Insert the eyepieces into the eyepiece tubes.
•If the rubber eye guards are to be used, fit them in the groove around the eyepiece.

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VMicroscopic procedure Interpupillary distance adjustment
•Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
•Adjust the interpupillary distance so that both the right and left field of view become one.
•This adjustment will enable the user to observe the specimen with both eyes
.
1Diopter adjustment
•Diopter adjustment compensates for differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the
extent to which focusing is lost when the objective magnification is changed. In particular,
this occurs when a low power objective is used.
•Before adjusting the diopter, bring a specimen into focus using the 10x objective.
•Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to
“0” position.
•Position 40x objective into the optical path and bring the specimen image into focus by turning
the coarse and fine focus knobs.
•Position either 4x or 10x objective into optical path. Without adjusting the fine and coarse focus
knobs, turn the diopter rings on the eyepieces so that the specimen images in the left and right
eyepieces are focused individually.
•Repeat the above step twice.
Diopter adjustment “0”
position

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2Centering the condenser
•Set the Phase annular diaphragm slider in centre position (O).
•Fully open the field of view diaphragm.
•Move the aperture diaphragm lever in open “O” position.
•Bring the specimen image into focus, using the 10x objective.
•Close the field of view diaphragm to its minimum setting.
•Turn the condenser focus knob so that the image of the field diaphragm forms on the
specimen surface.
•Adjust the condenser centering screws so that the centre of the field diaphragm image
matches the centre of the field of view. This adjustment is easier to make if the field
diaphragm size is stopped down to slightly smaller than the eyepiece field of view.
•For normal observation, the size of the diaphragm should be just outside the edge of the
field of view.
3Cetering the lamp
•Set the Phase annular diaphragm slider in the centre position (O).
•Fully open the field of view diaphragm.
•Move the aperture diaphragm lever to the open “O” position.
•Using the 10x Phase contrast objective, bring the specimen image into focus.
•Remove the diffuser filter slider from the light path.
•Remove an eyepiece and insert the phase centering telescope in its place.
•Holding the knurled part of the centering telescope, rotate its eyepiece to focus on the
phase plate image of the objective.
•Release the lamp socket clamp screw using the knob.

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•Move the lamp socket with the knurled knob to bring the lamp filament image to the centre of
the phase plate image of the objective.
•After finishing the above lamp centering procedure, insert the filter slider with mat surface of
the diffuser turned towards the user.
4Brightfield microscopy
•Set the Phase annular diaphragm slider in the centre position (O).
•Bring the specimen image into focus.
•Adjust the opening of the field of view diaphragm, for normal observation the size of the
diaphragm should be just outside the edge of the field of view.
•The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of
the illuminating system of the microscope. It is important because it determines the resolution
of the image, contrast, depth of focus and brightness.
•Stopping down the aperture diaphragm will lower the resolution and brightness but increase the
contrast and depth of focus. By stopping down the N.A. of the condenser to 2/3 of the N.A. of
the objective, a good image of suitable contrast will be obtained.
Lamp
S
ocket Clamp
Screw Knob

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5Phase-contrast microscopy
•Phase contrast objectives are labelled “Ph”: Ph1; Ph2; Ph3 and Ph4.
•For phase contrast microscopy, be sure to use the annular diaphragm that has the same
symbol as the objective, despite of the magnification of the objective.
•Fully open the aperture diaphragm.
•Bring the 10x (Ph1) objective into optical path.
•Position the Phase annular diaphragm slider to 10 -20. Set slider to 40 when using a
40x (Ph3) objective.
•Remove either eyepiece from the eyepiece tube and insert the phase centering telescope
in its place.
•Rotate the eyepiece of the centering telescope to focus on both the phase plate image of the
objective and the annular diaphragm image of the phase slider.
•If the objective phase plate and the annular of the slider do not coincide, use the two hexagonal
screwdrivers supplied with the microscope to bring the slider annular ring to the centre of the
phase plate, so that the image of the annular diaphragm is concentric with the phase plate image.
•If the slider annular ring image is moved from the phase plate image in the objective, a low
phase contrast image will result.
•For phase contrast microscopy at the maximum contrast, use GIF (Green interference filter)
in the optical path.
•Place the filter in the designated retaining ring above the phase annular diaphragm slider.

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VI Photomicrographic procedure
•The optical path selector lever can be used to set the optical path to either the Binocular tube 100:0
or Binocular tube/vertical tube 20:80 (observation: photo).
•Before starting photomicrography, check the following:
oThe condenser is centered.
oThe condenser annular diaphragm is centred.
oThe field of view diaphragm is stopped down to slightly just outside the edge of the
field of view.
•For photomicrographic procedures, refer to the manual of the specific camera being used.
Filter selection
Filter type Procedure
GIF (Green interference) 546nm For phase contrast and contrast adjustment with black and
white film
NCB (Neutral Colour Balance)
Blue For general microscopy and colour photomicrography
Never attempt either of the following actions, since doing so will damage the
focusing mechanism:
oRotate the left and right knob while holding the other.
oTurning the coarse and fine focus knobs further than their limit.

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VII Troubleshooting Table
As you use your microscope, you may occasionally experience problems. The troubleshooting table
below contains the most frequently encountered problems and their possible causes.
Optical and Operating Problems
Problem Possible Cause
Lamp not installed properly
Filter slider in intermediate position
Phase slider not in click-stop position
Incorrect condenser mounting
Condenser is set too low
Condenser is not centered
Field diaphragm closed too far
Aperture diaphragm closed too far
Revolving nosepiece not clicked
into position
Vignetting or uneven brightness
in the field of view or field of view
only partially visible
Optical path selector lever in
intermediate position
Aperture diaphragm closed too far
Field of view diaphragm image not
focused on specimen surface
Dust or dirt in field of view
Dust or dirt on specimen’s surface
Brightfield objective being used
Phase annular diaphragm not in
optical path
Phase annular diaphragm and
objective phase symbol do not match
Slider annular ring image has
moved away from the objective
phase plate image
Field of view diaphragm image not
focused on specimen surface
Image quality:
No image under phase contrast
or details cannot be viewed
Thickness of specimen holder is
outside the compensating range
of objective
Interpupillary distance not adjusted
Diopter adjustment not made
Inadequate illumination
Eye strain or fatigue
Field of view of left and right eyepiece differ
Electrical
Power supply not plugged in
Lamp not installed
Lamp does not light
Lamp burnt out
Inadequate brightness Specified lamp not being used
Lamp blows out immediately Specified lamp not being used
Connectors are not securely connected
Lamp near end of service life
Lamp flickers
Lamp not securely plugged into socket

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VIII Care and maintenance
1Lenses and filters
•To clean lens surfaces or filters, first remove dust using an air blower. If dust still persists, use a
soft/clean brush or gauze.
•A soft gauze or lens tissue lightly moistened with pure alcohol should only be used to remove
grease or fingerprints.
•Use petroleum benzine to clean immersion oil.
•Use petroleum benzine only to remove immersion oil from objective lenses.
•Because petroleum benzine and absolute alcohol are both highly flammable, be careful handling
around open flame.
•Do not use same area of gauze or tissue, to wipe more than once.
2Cleaning of painted or plastic components
•Do not use organic solvents (thinners, alcohol, ether, etc.). Doing so could result in discolouration
or in the peeling of paint.
•For stubborn dirt, moisten a piece of gauze with diluted detergent and wipe clean.
3When not in use
•When not in use, cover the instrument with vinyl dust cover and store in a place low in humidity
where mould is not likely to form.
•Store the objectives, eyepieces and filters in a container or desiccator with drying agent.
Note:
If equipment is used in a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
IX Warning lable
The following warning labels (or symbols) are found on the microscope, Study the meaning of the warning
labels (or symbols) and always use the equipment in the safest possible manner.
Warning Label / Symbol Explanation
Indicates that the surface becomes hot, and
should not be touched with bare hands.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Proper handling of the microscope will ensure years of trouble free service.
If repair become necessary, please contact your Motic agency or our Technical Service directly.