Motic K400 User manual

STEREO MICROSCOPE
MODEL K400 / K500 / K700
INSTRUCTION MANUAL

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Contents
1. Introduction …………………………………………………………….…..2
2. Nomenclature and Function…………………………………………..…..3
3. Assembly……………………………………………………..………….....5
4. Operating the Microscope……………………………………….……..…6
4.1 Interpupillary Distance Adjustment…………………………..………..…6
4.2 Diopter Adjustment………………………………………………………...6
4.3 Focusing………………………………………………………………….....7
4.4 Working Distance………………………………………………….……….8
5. Care of the Microscope………………………………………………...….8
6. Accessories………………………………………………….……………...9

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1. Introduction
A simple change in magnification for still objects has been provided in
stereomicroscopes developed in the recent past i.e. with a common primary
objective for the light in both tubes. The light beams do not project co-axially,
but diverge from the optical axis. Thus the two images produced are not
identical, giving a truly three–dimentional appearance.
In this adaptation, separated intermediate objectives are placed above
the common primary objective to produce the two beams and a magnified
image. The intermediate objectives, placed above the main objective in the
tube, can be easily manipulated to facilitate the change in magnification. It is
important that the distance from the object is not altered when the
magnification is changed.
A further refinement to this design is provided with the introduction of
pancratic intermediate objectives in the tube. The use of graduated changes
in magnification provides a continuous progression of magnification. The
most significant advantage of stereomicroscopes, with a common primary
objective, is that the distance between the objective and the object remains
constant at all magnifications.
To ensure correct usage, please read this manual completely before
operating the instrument.

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2. Nomenclature and Function
Model K 400 Stereo Microscope
Eyepiece
Diopter Ring
Diopter Scale
Binocular Eyepiece Tube
Magnification Changer
Objective
Focusing Knob
Focusing Mount
Stage Plate

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2. Nomenclature and Function
Model K 500 Stereo Microscope
Model K 700 Stereo Microscope
Eyepiece Tube
Clamp Screw
Zoom Magnification
Changer

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3. Assembly
1. The base plate of the incident light stand (and the base of the
transmitted light stand with light source) with support column, in their
standard variants, are a typical working stand. The focusing mount
moves along the vertical column and is fixed in position by the focusing
mount clamp screw. The safety support collar maintains the adjusted
height and is secured below the focusing mount by the clamp screw
along the vertical column.
2. Tilt the binocular eyepiece tube and mount it on the optical head with
the magnification changer - aligning the groove with the positioning pin
and fasten the eyepiece tube clamp screw.
The binocular tube may be turned through 180° and each eyepiece
tube is equipped with a diopter adjustment ring.
3. Insert the eyepieces into the eyepiece tubes. Set the diopter scale on
each eyepiece to “0”.
4. Fit the stage plate, while pushing
it against the clip, in the rim of the
opening in the base.
The black or opal surface is to be
used according to the specimen
to be observed. For transmitted
light base, use the ground glass
plate.

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4. Operating the Microscope
4.1 Interpupillary Distance Adjustment
This adjustment should be
performed every time the
observer changes as the
interpupillary distance differs
between the individuals.
Interpupillary distance is changed
by simply spreading the eyepiece
tubes. They will hold their position
at the selected setting.
This will allow the view field of
each eye to merge into one.
Interpupillary distance values
range from 54mm and 76mm.
4.2 Diopter Adjustment
Both eyepieces can be focussed independently to compensate for refractive
differences of the eyes.
a. Turn the diopter rings on both eyepieces to set them at the “0”
position.
b. Turn the magnification changer to maximum. Focus on the sample
using the focus knob.
c. Turn the magnification changer to minimum. Look through the left
eyepiece with the left eye, focus on the specimen using the diopter
ring on the left eyepiece. Then, look through the right eyepiece
with the right eye and focus on the specimen using the diopter ring
on the right eyepiece.
d. Repeat steps 2.2 and 2.3 until the image is kept focused even
though the magnification is changed. This adjustment ensures
sharp image throughout the magnification change.

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4. Operating the Microscope
4.3 Focusing
The focus knobs are conveniently
shaped and allow accurate
focusing over a 50mm range.
The ease of movement can be
matched to the load and to your
personal needs.
Focus knob torque adjustment
It is possible to adjust the torque
"tension" of the focus knob.
To increase the torque, turn either of the torque adjustment rings
located behind the focus knobs - after slackening the Hex set screw
turn the right ring in the clockwise direction - turn the left ring in the
counter clockwise direction after slackening the Hex set screw. To
reduce the torque turn the rings in the direction opposite to the above.
Do not reduce the torque too much since the optical body will fall of its
own weight.
The roller bearing slide of the focusing mount is maintenance free.

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4. Operating the Microscope
4.4 Working Distance
The distance between the upper surface of the specimen and the
bottom of the objective, when the specimen is in focus, is called the
working distance of that objective. The working distance changes when
the objective magnification changes and when auxiliary objectives are
attached. Generally, the working distance becomes shorter as the
objective magnification increases.
When observing a particularly thick specimen, the distance between the
upper surface of the specimen and the objective may be shorter than
the working distance of the objective. If this occurs, loosen the focusing
mount clamp screw and slide the focusing mount to the upper end of the
column.
5. Care of the Microscope
Keeping all optical components clean is important for good optical
performance. The microscope should always be covered with the vinyl dust
cover provided with the instrument when not in use.
Dust on the surface of lenses may be removed by the use of a soft lens
brush i.e. before attempting to wipe the surface clean.
Persistent fingerprints and oily smears on eyepieces and other microscope
body parts may be removed with soft cloth moistened with absolute alcohol.
Painted and plastic surfaces should be cleaned with mild detergent solution.
Store the microscope in a room free from humidity and corrosive fumes.

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6. Accessories
Eyepieces
Micrometer Eyepieces
6.25X/F.N.23, 10/X/F.N.21, 15X/F.N.17.6,
20X/F.N.13.4, UW10XF.N./23, 32X/F.N.12.
10X/F.N.23 360° Protractor,
10X/F.N.23 14mm:0.2mm
10X/F.N.23 14mm:0.1mm
20XF.N.13.4 10mm:0.1mm
Auxiliary Objectives 0.3X, 0.5x, 0.625X, 1.5X, 2.0X,
Base/Stand Plan stand, Transmitted light stand, Universal
table stand, Universal clamp stand, Articulating
arm table stand, Articulating clamp stand.
Stage Inserts Gliding Stage
Photographic and
CCTV
Beam Splitter with Double iris for Photography
K400
Beam Splitter with Double iris for Photography
K500/K700
Beam Splitter with Double iris for CCTV K400
Beam Splitter with Double iris for CCTV
K500/K700
Drawing Tube For K400 can be mounted in two positions
180° apart thus suitable for left and right-hand
observers.
For K500/K700 can be mounted in two
positions 180° apart thus suitable for left and
right-hand observers.
Observation Options Dual Teaching Head with Electronic pointer
Illuminators Epi-illuminator, Fluorescent ring light,
Fiber-Optics bifurcated illuminator