Motic AE30-31 User manual

MOTIC INCORPORATION LTD.
Prepared by Motic Instruments Inc. Canada
Episcopic-Fluorescence Attachment EF-INV-ll
Inverted Microscope AE30-31
Instruction Manual

Motic Instruments Inc. Canada
October 06, 2006
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This manual may not be reproduced or transmitted in whole or in part without
Motic’s express permission.
Fluorescence
This instruction Manual has been prepared for users of the
AE-INV-ll Epi-Fluorescence Attachment used in conjunction
with the Motic AE30/31 Inverted Microscope.
We are constantly endeavouring to improve our instruments and
to adapt them to the requirements of modern research
techniques and testing methods. This involves modification to
the mechanical structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction
manual, including all specifications are subject to change
without notice.

Motic Instruments Inc. Canada
October 06, 2006
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The optical phenomenon that occurs when light absorbed by a material, creates a molecular
excitation that causes the material to re-emit light at a different wavelength.
Fluorescence Microscope for Epi-Fluorescence
The technique of fluorescence microscopy with epi-illuminators is based on the adaptation of
the vertical illuminator used in reflected light microscopy. The radiation emitted by the light
source first passes through the exciting filter and is incident to a short-pass filter* (dichroic beam
splitter). The radiation is then reflected into the objective, which also serves as a condenser.
The objective concentrates the exciting radiation in the object field. Fluorescent light emitted
from there is collected by the objective and returned in the opposite direction to the short-pass
filter, which directs it into the eyepiece through the barrier filter.
*A filter designed to allow the passage of radiation of wavelengths shorter than a given limit.

Motic Instruments Inc. Canada
October 06, 2006
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Table of Contents
1.0 Names of Component Parts
2.0 Setting up the Instrument
3.0 Assembly
4.0 Microscopy
5.0 Troubleshooting Table
6.0 Care and Maintenance

Motic Instruments Inc. Canada
October 06, 2006
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1.0 Names of Component Parts
Fig. 1
Epi-fluorescence
attachment EF-INV-ll
Light source Epi-fluorescence attachment
Light protective shield
Power supply unit

Motic Instruments Inc. Canada
October 06, 2006
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2.0 Setting up the Instrument
Avoid placing the instrument in areas exposed to direct sunlight, dust, vibration, high
temperature and high humidity
3.0 Assembly
3.1 Microscope assembly
Follow the instructions in the microscope manual.
For the purpose of performing simultaneous observation with phase contrast, attach the
appropriate phase contrast components referring to the specific instructions provided.
3.2 Installing the Epi-fluorescence attachment
Required tools
Allen hex keys: 1.5mm, 2.5mm and 5mm (supplied with attachment).
Please see illustrations while assembling the attachment
Turn off the microscope power switch and unplug the power cord
Before Starting

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October 06, 2006
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3.3 Protective dust covers
See Figure 2
Pull out rear dust cover, located above the Microscope AC inlet.
Fig. 2
See Figures 3a and 3b
Remove side dust covers located on each side of the microscope base.
Fig. 3a Fig. 3b
Microscope AC inlet
Rear dust cover
Right side cover
Left side cover

Motic Instruments Inc. Canada
October 06, 2006
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3.4 Attaching the Epi-fluorescence attachment sleeve
See Figure 4
Attach the main body sleeve of the epi-fluorescence attachment into the microscope
stand, with the notch on the sleeve facing left.
Fix the main body sleeve in this position by using the three 5mm Allen hexagonal
screws.
Fig. 4
Epi-fluorescence attachment
main body sleeve
Notch facing left

Motic Instruments Inc. Canada
October 06, 2006
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3.5 Mounting the Epi-fluorescence attachment main body
See Figure 5
Align the pin on the Epi-fluorescence attachment with the notch in the Epi-
fluorescence attachment sleeve, slide the main body of the Epi-fluorescence
horizontally into the sleeve until it cannot be pushed in any further. To secure,
tighten the fixing screws using a hexagonal Allen key.
Fig. 5
Shutter slider with
Filter holder

Motic Instruments Inc. Canada
October 06, 2006
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3.6 Installing the shutter slider with the filter holder
See Figure 6
•Loosen one of the slider knobs.
•With the filter slider slot to the left and facing the operator, insert the slider into the
opening of the main body of the fluorescence attachment.
•Tighten the loosened knob.
Fig. 6
Light cut-off slider
•To prevent the specimen from fading during temporary pauses in observation, slide
the shutter to the left.
•When using transmitted light illumination, slide the shutter to the left.
Block the optical path of the Epi-fluorescence by sliding
the shutter to the left.
Shutter slider open position
Filter slot