Motic M3600 Series User manual

M3600 Series Instruction Manual
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Motic Swiftline 3600 Series
Stage Clips
Camera Port
Head
Arm
Cord Holder
Coarse Focus
Knob
Fine Focus
Knob
Mechanical
Stage Control
Light Intensity
Control
Eyepiece
Nosepiece
Objective
Specimen
Holder
Stage
Iris Diaphragm
Illuminator
On / Off Switch

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Microscope Components
ARM - the vertical column (attached to the base) which supports the
stage and contains the coarse and fine adjusting knobs and focusing
mechanism.
BASE - the housing and platform of the instrument to which the arm is
attached. The base stands on rubber feet and contains the illuminator
assembly. The bulb replacement part number is printed on the
underside of the base.
COARSE FOCUS CONTROL - the larger focus control knob located on
either side of the microscope which facilitates rapid and heavy
movement of the focusing mechanism.
COAXIAL CONTROLS - the focusing mechanism moves the stage up and
down to bring the specimen into focus. The coaxial focusing system
combines both the coarse and fine focus into one knob located on both
sides of the microscope. The control is designed for a continuous
operation over the range of the stage movement.
CONDENSER –the condenser is mounted in the stage and it is used in
conjunction with the iris diaphragm. The function of the condenser is to
provide full illumination to the specimen plane and to enhance the
resolution and contrast of the object being viewed. The condenser can
be raised and lowered for precise light control.
CORD HOLDER (Corded models only) - the bracket installed on the back
of the arm which is used to wrap and store the electrical cord.
EYEPIECES - the upper optical element that further magnifies the
primary image of the specimen and brings the light rays in focus at the
eye point.
FINE FOCUS CONTROL - the fine focusing knobs, located on either side
of the microscope, on the sides of the coaxial focusing control. They are
used for precise focusing adjustments once the specimen has been
brought into view with the coarse focus controls.
HEAD - the top portion of the microscope that contains the refracting
prisms and the eyepiece tube. The eyepiece is locked onto the eyepiece
tube with a set screw
CAMERA PORT - the top portion of the microscope is equipped with an
optional camera port. This port allows the end user to attach a digital
camera (such as a Moticam), with the purchase of the optional C-Mount
camera lens (MA15604). Ask your microscope dealer for details. To
remove the cap, a 1.5mm hex wrench is will be required (not
provided).

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ILLUMINATOR –the built-in light source which provides the optical
system with light.
ILLUMINATOR INTENSITY CONTROL –Controls the illuminator’s
brightness.
IRIS DIAPHRAGM - a round multi-leaf device mounted below the
condenser which is controlled by a lever. It is similar to a camera
shutter and controls the amount of light entering the condenser,
allowing the user to control contrast.
MECHANICAL STAGE - an alternative to stage clips is a mechanical
stage. A mechanical stage holds the slide in place, allowing the user to
move the slide on any x/y axis through the manipulation of two control
knobs or a co-axial control mechanism.
NOSEPIECE - the revolving turret that holds the objective lenses,
permitting changes in magnification by rotating different powered
objective lenses into the optical path. The nosepiece must “click” into
place for the objectives to be in proper alignment.
OBJECTIVES - the DIN objectives are standard in the industry, with large
numerical aperture (N.A.) to permit maximum resolution. All objectives
are color-coded and parfocaled. The 40XRD and the 100XRD are sealed
to keep oil residue from seeping into the objective. These two
objectives have a spring loaded tip to prevent accidental breakage of
either the slide or front lens of the objective if they should come into
contact with each other.
ON/ OFF SWITCH - turns the illuminator on or off.
STAGE - the table of the microscope where the slide is placed for
viewing. This component moves upward and downward when the
focusing knobs are turned.
STAGE CLIPS - a pair of flexible metal clips attached by spring screws
that hold the slide in position on the stage.
IMPORTANT MICROSCOPY TERMS
APERTURE, ANGULAR - the angle (or cone) of light rays capable of
entering the front lens of the objective from a point in the object. By
increasing the angular aperture of an objective, more light rays from
the specimen can be taken in by the lens; hence the resolving power is
increased.
COMPOUND MICROSCOPE - a microscope having a primary magnifier
(the objective) and a second (the eyepiece) to both conduct light,
amplify magnification and convert the image into a field of view easily
seen by the human eye.

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COVER GLASS - thin glass cut in circles, rectangles or squares, for
covering the specimen, usually a thickness of 0.15 to 0. I7mm. The
majority of specimens should be protected by a cover glass, and must be
covered when using 40XRD or 100XRD objectives.
DEPTH OF FOCUS - the ability of a lens to furnish a distinct image
above and below the focal plane. Depth of focus decreases with the
increase of numerical aperture or with the increase of magnification.
DIN –(Deutsche Industrial Norman) an international optical standard for
the manufacturing of most quality microscope lenses. Many DIN lenses
will be interchangeable from one DIN microscope to another.
EYE POINT or EYE RELIEF –the distance from the eyepiece lens to your
eye where a full field of view can be seen.
FIELD OF VIEW - the area of the object seen when the image is
observed. It may range in diameter from several millimeters to less than
0.1mm.
FOCAL LENGTH - parallel rays of light after refraction through a lens
will converge to a focus at the focal point. The distance from the
optical center of the lens to the focal point is the focal length.
NUMERICAL APERTURE (NA) –a measure of an objective’s light
gathering capabilities. The concept may be compared to the F-valve in
photographic lenses. Generally speaking, objectives with N.A. values of
less than 1.00 are "Dry" objectives. Values of 1.00 or greater require oil
as a medium. Please note that condensers are part of the optical system
and are also assigned an N.A. value. That value must be at least as high
as that of the highest objective used.
PARFOCAL - a term applied to objectives and eyepieces when
practically no change in focus has to be made when an objective of one
power is substituted for another. The DIN objectives on your Swift
M3600 Series Microscope are parfocaled at the factory to a standard
45mm distance, so that only a slight turn of the fine adjustment is
required when a change is made from a lower to higher power.
RESOLVING POWER - the ability of a lens to clearly separate fine detail.
Resolving power is directly proportional to the numerical aperture of
the optical system.
WORKING DISTANCE –the distance from the lens of the objective to the
cover slip on the slide, when the specimen is in focus.
CORDLESS OPERATION (Cordless models)
The rechargeable battery should be fully charged for approximately 8
hours before the initial use. It can be charged by using the 4.5 volt A/C
adapter included with the microscope. The battery can be used to

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power the illumination system for approximately 40 hours. If the
microscope is used in the same location, the A/C adapter can remain
plugged-in without damage to the battery or recharging system.
A battery power indicator meter is located in the front of the base.
Each blue LED light indicates the level of remaining battery power.
When the battery is fully charged, all 5 LED lights will be illuminated.
The battery will need to be recharged when there is only 1 illuminated
LED remaining on the meter.
USING YOUR SWIFT MICROSCOPE
1. Use the stage clips or slide holder mechanism to secure the slide in
place. Be sure the specimen is centered over the opening in the
stage.
2. Rotate the nosepiece to place the lowest power (4X) objective over
the specimen. Be sure the objective “clicks” into position.
3. Turn on the illumination by pressing the on/off switch towards “1”
4. Open the iris diaphragm to its largest aperature.
5. While viewing through the eyepiece(s), rotate the coarse focus knob
slowly and carefully to bring the specimen into focus. The specimen
may require some centering in the field of view at this time. By
using the fine focusing knob, slowly and carefully refine the focus to
clearly observe the fine details of the specimen.
6. If the image of the specimen appears pale, the aperture of the iris
diaphragm should be slowly closed until the details of the specimen
are sharply defined. If the specimen appears dark, slightly open the
diaphragm.
Please note: a smaller iris diaphragm aperture (opening) increases the
contrast in the image while a larger aperture decreases the contrast.
(The diaphragm is not intended for controlling the brightness of the
illumination). A good procedure to follow in selecting the proper
opening is to start with a large aperature and reducing it until the fine
detail of the specimen is in exact focus. Using an inappropriate aperture
results in a “washing out” of the image. Care must be exercised not to
reduce the aperture too much to gain high contrast, as then the fine
structure in the image of the specimen will be destroyed. Reducing the
aperture increases contrast and depth of focus, but it also reduces
resolution and causes diffraction. Example: The aperture for the 10X
objective will not be the same as for the 40XRD objective, since the
angle of the required light is determined by the numerical aperture
(N.A.) of the objective. The proper aperture of the diaphragm can be
easily achieved after minimal experience with the microscope.

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8. Rotate the nosepiece to the next higher power objective. A slight
turn of the fine focusing knob may be required to bring the image of
the specimen into sharp focus. Once the specimen is in focus with
the highest power objective, it will be in focus with each lower
power objective.
OIL IMMERSION (Only for models with a 100X objective)
When light rays pass through air from the specimen to the objective
lens, they are distorted slightly, a phenomenon known as refraction.
This is usually not a problem at a magnification of 400X or lower.
However, at a magnification of 1,000X and above, refraction becomes
problematic. This problem is reduced significantly by placing a thin
layer of very clear, viscous oil between the slide and tip of the objective
lens. The result is a much clearer image at 1,000X because the oil has
the same light transmitting properties as glass. Using oil slightly
increases the resolution and brightness of the image. Usually a very thin
slide (size #1) is used for oil immersion because at this magnification,
the working distance is very small and is critical to focusing the
specimen. Good quality glass (not plastic) cover slips should be used. If
their thickness is over 0.17mm, the objective will not resolve properly,
because the specimen cannot be moved close enough to the objective
lens to be in focus.
Place a tiny amount of oil (only 1 drop should be sufficient) onto the
slide prior to rotating the 100XRD objective into position. It is essential
to thoroughly clean the objective tip after use to prevent damage and
to ensure that an image can be seen clearly the next time the objective
is used.
IMPORTANT: The working distances of the 40XRD and 100XRD objectives
to the slide surface are very small and although these objectives are
sealed to prevent oil contamination, it is a good practice to avoid
dragging these objectives through an oiled slide. The 100XRD oil
immersion lens on Swift microscopes has a spring-loaded end to prevent
cracking the cover slip upon its initial contact. Once this zone of safety
is exceeded by moving the slide further toward the lens, a point can be
reached where damage will occur. Always make a practice of frequently
checking the position of the lens on the slide. Note that a 100XRD
objective requires an iris diaphragm for brightfield oil immersion
microscopy.

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HOW TO USE THE POINTMASTER® EYEPIECE RETICLE
Swift's patented POINTMASTER® eyepiece reticle, which is installed in
the eyepiece of the microscope, enables the user to easily measure the
size of the specimen
I. The numbers on the chart indicate the actual size of the
POINTMASTER® scale in millimeters. For example, the length is
1.0mm from the top of the arrow to the bottom; the total length of
the scale is 8.0mm, etc. The thickness of all the horizontal lines is
0.01mm.
2. To obtain the actual physical size of a specimen, divide the
POINTMASTER® scale readings by the magnification of the objective
lens that is in use. Divide the readings by 10 if the 10X objective is
being used, 40 if the 40XRD objective is in use, or 100 if of 100XRD
is in use.

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ADDING A DIGITAL CAMERA TO TOP CAMERA PORT
The top port of your M3600 series microscope allows the attachment of
a digital camera, such as the Moticam camera. The purchase of an
optional C-mount camera lens is necessary for the conversion
(MA15604).
1. First take your Moticam camera and remove the dust cap from
the bottom of the camera, being careful not to introduce dust.
2. Then take the MA15604 C-mount lens and attach it to the
Moticam camera. Then set to the side.
3. Remove the top camera port cover of the microscope, by
loosening the three 1.5mm hex screws. 1.5mm hex wrench
purchased separately.
4. Take the Moticam with attached C-Mount and slide down into
the open top camera port.
5. Tighten the three 1.5mm hex screws back around the C-mount
to secure it in place.
6. Then follow the camera instructions.
CARE AND CLEANING
Motic Swiftline microscopes are designed to function with minimal
maintenance, but certain components should be cleaned frequently to
ensure ease of viewing. The power switch should be turned off or the
microscope should be unplugged when not in use.
CLEANING –The front lens of the objectives (particularly the 40XRD and
100XRD) should be cleaned after use. The lens surface may be gently
cleaned with a soft camel hair brush, or blown off with clean, oil-free
air to remove dust particles. Then wipe gently with a soft lens tissue,
moistened with optical cleaner (eyeglass or camera lens) or clean
water. Immediately dry with a clean lens paper.
CAUTION - Objectives should never be disassembled by the user. If
repairs or internal cleaning should be necessary, this should only be
done by qualified, authorized microscope technician. The eyepiece(s)
may be cleaned in the same manner as the objectives, except in most
cases optical cleaner will not be required. In most instances breathing
on the eyepiece to moisten the lens and wiping dry with a clean lens
tissue is sufficient to clean the surface. Lenses should never be wiped
while dry as this will scratch or otherwise mar the surface of the glass.

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The finish of the microscope is hard epoxy and is resistant to acids and
reagents. Clean this surface with a damp cloth and mild detergent.
Periodically, the microscope should be disassembled, cleaned and
lubricated. This should only be done by a qualified, authorized
microscope technician.
DUST COVER AND STORAGE –All microscopes should be protected from
dust by a dust cover when in storage or not in use. A dust cover is the
most cost-effective microscope insurance you can buy. Ensure that the
storage space is tall enough to allow the microscope to be placed into
the cabinet or onto a shelf without making undue contact with the
eyepieces. Never store microscopes in cabinets containing chemicals
which may corrode your microscope. Also, be sure that the objectives
are placed in the lowest possible position and the rotating head is
turned inward and not protruding from the base. Microscopes with
mechanical stages should be adjusted toward the center of the stage to
prevent the moveable arms of the mechanical stage from being
damaged during storage in the cabinet.
LED REPLACEMENT - To prolong the life of the bulb you should always
turn off the unit when not in use. The replacement LED part number can
be found underneath the microscope on the metal base plate. The
M3600 models use a .5W LED (Part Number MA14788).
COMMON PROBLEMS IN MICROSCOPY
CAUTION –Never disassemble mechanical or optical components. This
servicing should only be done by an authorized Swift technician. The
Limited Lifetime Warranty will be null and void if the mechanical or
optical components are disassembled by a non-Swift dealer.
A. PROBLEM –No Illumination
CORRECTION -
1. Is the power plug connected to an active A.C. outlet?
2. Is the on/off power switch working properly?
3. Turn the illuminator rheostat to a brighter setting.
4. Check the bulb. Try a new bulb if you have one.
B. PROBLEM –Illumination “hot spots” and uneven brightness in the
field of view.
CORRECTION -
1. Is the Abbe condenser in the correct position?
2. Is the nosepiece and objective clicked into proper position?
C. PROBLEM –Image appears “washed out” or weak.
CORRECTION -

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1. Slightly close the diaphragm to a smaller aperture
2. Objective lens is dirty. See “Care and Cleaning” Section.
3. Eyepiece is dirty. See “Care and Cleaning” Section.
D. PROBLEM –Dust or hairs seem to be moving in the image.
CORRECTION –The iris diaphragm is not open wide enough. Slowly
open the diaphragm to increase the size of the opening allowing
for additional illumination.
E. PROBLEM - Once the specimen is in focus, it moves out of focus.
CORRECTION –Gravity is causing the stage to drift downward,
causing loss of focus. The focusing tension should be increased to
act as a brake to prevent this downward drift
F. PROBLEM –Focusing knobs turn with difficulty.
CORRECTION –
1. Loosen the tension collar adjustment. See page 10.
2. The microscope should be disassembled, cleaned and
re-lubricated by a qualified, authorized technician.

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Motic Hong Kong Limited (HONG KONG)
Unit 2002, L20, TowerTwo, Enterprise Square Five, 38 Wang Chiu Road, Kowloon Bay,Kowloon,
Hong Kong Tel: [phone] Fax: [phone]
Motic Instruments Inc. (CANADA)
130-4611 Viking Way Richmond, B.C.
V6V 2K9 Canada Tel: [phone]
Fax: [phone]
Motic Deutschland GmbH (GERMANY)
Christian-Kremp-Strasse 11, D-35578
Wetzlar, Germany Tel: [phone]
010 Fax: [phone]
Motic Europe (SPAIN)
C.Les Corts 12, Pol. Ind. Les Corts. 08349 Cabrera de Mar,
Barcelona, Spain Tel:[phone] Fax [phone]
Motic Hong Kong Limited Copyright © 2010-2017. All Rights Reserved.
Design Change : The manufacturer reserves the right to make changes in instrument design in
accordance with scientific and mechanical progress, without notice and without obligation.