Motic BA410 Series User manual

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We are constantly endeavouring to improve our instruments and to adapt them to the
requirements of modern research techniques and testing methods. This involves
modification to the mechanical structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all
specifications are subject to change without notice.

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INFINITY CORRECTED OPTICS
In this optical concept the light beams are parallel after leaving the objective in the
direction of the eyepieces. A second optical element, the tube lens (normally located in
the eyepiece tube) is used to converge the parallel beams, resulting in an intermediate
image. The intermediate image is focussed by the eyepieces, to provide the real image
for visual observation.
The implementation of a tube lens gives the opportunity to minimize chromatic
aberrations and other “optical defects”. Further, in “Infinity Optics” the distance between
the objective and tube lens is not as strictly fixed as in the (historically older) “Finite
Optics” of 160mm tube length.
This allows additional optical components to be inserted between the objective and tube
head.
Fluorescence attachments, discussion bridges, eye level risers and other options can be
added without affecting the image quality.
In general “Infinity Optics” provides flexibility and the opportunity to add additional
optional features.

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TABLE OF CONTENTS
Section
I Nomenclature 4
II Setting up the instrument 7
Operating environment 7
III Assembling the microscope 7
Verifying Input voltage 7
1 Mechanical stage 8
2 Specimen holder 8
3 Objectives 8
4 Condenser 9
5 Eyepiece tube 9
6 Eyepieces 10
7 Illumination 10
8 Filters 11
9 Power cord 12
IV Microscopy 12
1 Coarse and fine focusing 12
2 Coarse focus torque adjustment 12
3 Coarse focus quick stop 13
4 Stage upper limit stop adjustment 13
5 Beam splitter lever 14
6 Interpupillary distance adjustment 15
7 Diopter adjustment 15
8 Centering the condenser 16
9 Use of aperture diaphragm 17
10 Use of field diaphragm 18
11 Brightness and contrast adjustment 18
V Photomicrographic procedure 19
VI Using Oil Immersion Objectives 20
VII Troubleshooting Table 21
VIII Care and maintenance 23
IX Warning label 26

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I. Nomenclature
MOTIC BA410 (30W Halogen)

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MOTIC BA410 (Trinocular) 30W Halogen

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MOTIC BA410 30W Halogen

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II. Setting up the instrument
Avoid placing the instrument in locations exposed to direct sunlight, dust, vibration, high
temperature, high humidity and where it is difficult to unplug the power supply cord.
Operating environment
yIndoor use
yAltitude: Max 2000 meters
yAmbient temperature: 15°C to 35°C
yMaximum relative humidity: 75% for temperature up to 31ºC decreasing linearly to
50% relative humidity at 40ºC
ySupply voltage fluctuations: Not to exceed ±10% of the normal voltage
yPollution degree: 2 (in according with IEC60664)
yInstallation / Overvoltage category: 2 (in according with IEC60664)
yAir Pressure of 75kPa to 106kPa
yAvoid frost, dew, percolating water, and rain
III. Assembling the microscope
Verifying Input voltage
yThe automatic voltage selection works with a broad range of settings. However,
always use a power cord that is rated for the voltage used in your area and that has
been approved to meet local safety standards. Using the wrong power cord could
cause fire or equipment damage.
yIf using an extension cord, use only a power supply cord with a protective earth (PE)
wire.
yIn order to prevent electric shock, always turn the power supply off before connecting
the power cord.

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yElectrical Specifications:
30W Halogen Bulb
Input: 90-240V~, 80VA, 50-60Hz
Bulb: 6V 30W Halogen
Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)
1. Mechanical stage
yLoosen the stage clamp screw; fit the stage into the circular dovetail mount. An
orientation notch at the back of the stage has to fit to an orientation pin at the stage
carrier. Tighten with the clamp screw facing forward.
yTorque adjustment
The torque of the y-axis and x-axis travel knobs can be adjusted by torque
adjusting knobs.
For y-axis to (+) increase or (-) decrease the torque, turn Knob Y while holding A
For x-axis to (+) increase or (-) decrease the torque, turn Knob X while holding B
2. Specimen holder
yAttach the specimen holder, using the two mounting holes. If the stage is to be used
with right right-hand controls, attach the specimen holder to the left-hand holes and
similarly, with left-hand controls to the right-hand holes.
3. Objectives
yLower the stage completely. Screw the objectives into the revolving nosepiece so
that clockwise rotation of the nosepiece brings the next higher magnification objective
into position.

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4. Condenser
yRaise the stage by turning the coarse focus knob.
yCompletely lower the condenser carrier by turning the condenser focus knob.
yInsert the condenser into the dovetail mount with aperture scale facing forward
towards the user. Secure it with the condenser clamp screw.
yTurn the condenser focus knob to raise the condenser as far as it will go.
1. Condenser focus knob
5. Eyepiece tube
Loosen the eyepiece tube clamp screw. Insert the round dovetail mount on the
eyepiece tube into the round dovetail mount on the microscope arm. Tighten the
eyepiece tube clamp screw to secure the eyepiece tube in place.
1. Eyepiece Tube Clamp Screw