MP superfastprep-2 User manual

mpbio.com/sampleprep
Cat. No.
116012500
For Research Use Only
Protocol Revision #116012500-15OCT
Owners Manual

2mpbio.com
Table Of Contents
Precautionary Instructions........................................................................3
Electrical Safety........................................................................................................3
Safety Equipment .....................................................................................................3
Operation Precautions..............................................................................................3
Introduction ...........................................................................................................4
Product Contents...............................................................................................4
Picture of Contents..........................................................................................5
Super FastPrep-2TM Operating Instructions ...................................6
Battery Charging.......................................................................................................6
Holding the Super FastPrep-2TM ...............................................................................6
Sample Processing...................................................................................................7
Cleaning ...................................................................................................................9
Warranty and Disclaimer............................................................................10
It is recommended to keep the original packaging in case you need to return the
product for any reason to your distributor or MP Biomedicals.
Note: MP Biomedicals is not responsible for any harm or damage resulting directly/indirectly from the use of
the Super FastPrep-2 or related product components, materials, or technologies.
Some reagents in kits are irritants. Wear protective gloves and eye protection and follow the safety guidelines
and rules enacted by your research institution or facility. Refer to product/reagent specic MSDS information
from the manufacturer.

3
PRECAUTIONARY INSTRUCTIONS
Be sure to read all safety instructions in this manual carefully and thoroughly. Pay
attention to warnings before using the Super FastPrep-2. Failure to follow all
instructions may lead to injury.
Warning: Indicates a potential hazard that should be avoided.
Electrical Safety
Avoid contact with grounded surfaces.
Outlets for charging must match the plug on the battery charging station.
A voltage converter is included for countries that use 220-240 VAC.
Do not use the Super FastPrep-2 in the rain or under wet conditions.
Do not misuse the cord.
Safety Equipment
aThe Super FastPrep-2 can be very loud so it is recommended to use ear
protection during operation.
aAlways use eye protection while operating the Super FastPrep-2.
For further safety information regarding the rotary tool, battery, and charging station
please see accompanying literature.
Operation Precautions
Operation of the Super FastPrep-2 with a plastic tube at full speed for more
than 10 seconds may cause the grinding matrices within the tube to overheat,
leading to sample degradation and/or tube failure. If grinding of a sample for
over 10 seconds is desired, lower speeds should be used and/or periodic stopping
and cooling of the tube should be performed.
When using metal or ceramic grinding matrices greater than 2mm in diameter,
do not operate at a speed greater than 25 (4,000 cycles per minute) or tube
failure may result.
Although the modular design of the Super FastPrep-2 allows it t on many off-
the-shelf rotary tools from different manufacturers, using the Super FastPrep-2
with a rotary tool with an electric stop (stops immediately after switching it off) will
damage the Super FastPrep-2 and void the warranty. Use the Super FastPrep-2
only with the rotary tool supplied, which has been specically modied to disable its
electric stop and coast to a stop when switched off.

4
INTRODUCTION
The Super FastPrep-2 is an attachment for a rotary tool developed for vigorous cell
disruption (bead beating) and allows the researcher/investigator to process samples
in the eld, at the site of sample collection. It can be used to lyse microbes in soil,
sediment, sludge, and fecal samples and can effectively process tough-to-lyse fungal,
algal, plant, and animal tissues. It can be used at any remote location and in most
weather conditions when immediate sample collection, processing, and preservation are
required by the researcher. The device is compatible with most 2 mL tubes containing
lysis matrix.
The Super FastPrep-2 consists of a unique, reciprocating grinding mechanism
mounted on a rotary tool. It is designed to operate at 600 to 4,800 cycles per minute as
long as charge remains in the battery. This allows the researcher to maintain control of
the speed and time of sample lysis. Overall, the Super FastPrep-2 is ideal for
cordless, portable, and convenient processing of tough-to-lyse biological and
environmental samples, both in the lab and in the eld.
PRODUCT CONTENTS
1) One (1) Super FastPrep-2
2) One (1) Rotary tool
3) Two (2) Lithium Ion Rechargeable Batteries
4) One (1) Battery Charging Station
5) Ten (10) sample tubes
6) Four (4) tube support plugs
7) Product Manuals
8) 220-240 VAC to 100-120 VAC Voltage Converter to countries where necessary

5
Charging
Station
Rotary
Tool
Lithium Ion
Rechargeable
Batteries (2)
Lysing
Matrix
Tubes
Support
Plugs
Super FastPrep-2TM Unit Voltage converter
220-240 VAC to 100-120VAC

6
OPERATING INSTRUCTIONS
Battery Charging
Connect the battery charging station to an appropriate power outlet (use the power
converter to use the charging station outside of North America)
Place the battery in the charging station by aligning the raised ribs of the battery pack
with the slots in the charging station. The light on the charging station should ash green
while the battery is charging. The battery pack is fully charged when the green light
stops ashing and remains on.
Holding the Super FastPrep-2
During operation, hold the Super FastPrep-2 as in the picture below.

7
The angular position of the rotary tool relative to the Super FastPrep-2 may be adusted
by removing the 3 screws at the base of the Super FastPrep-2, rotating the adapter to the
desired position, and then replacing the 3 screws. See the picture below.
Sample Processing
aSample Sources – The Super FastPrep-2 device can be used to effectively lyse
microbes in soil, sediment, sludge, and fecal samples and can effectively process tough-
to-lyse fungal, algal, plant, and animal tissue. The Super FastPrep-2 is compatible with
skirted, 2 mL tubes. Two mL tubes having conical bases are compatible with the Super
FastPrep-1, however support plug (included) must be installed to help prevent failure of
those tubes.
aOperating Temperatures – (Optimum -20oC to 45oC) Severe cold conditions
can drain the battery and affect the number of samples that can be processed with the
Super FastPrep-2. Keeping the battery warm (e.g., in the pocket of a jacket or parka)
prior to use can extend battery life in colder climates and operating conditions.
aProcessing Time and Speed – (Generally <5 seconds at 4,000 cycles per
minute) The extent of sample lysis will vary according to the processing speed and time.
Due to the extreme pulverizing forces generated by the Super FastPrep-2, the tempera-
ture of samples can increase as a function of lysis speed and time. This may

8
affect the integrity of DNA/RNA/protein released from biological materials. If longer
processing times (>5 seconds) are required, it is recommended to let the sample cool
on ice for a minimum of two minutes before continuing with sample processing and/or to
reduce the lysis speed.
1. Remove Super FastPrep-2 and rotary tool from the box or carrying case.
2. Screw rotary tool into Super FastPrep-2 to a tight t.
3. Open lid and insert a screw-cap 2mL tube into the holder making sure that
it snaps in securely. If you are using one or more ¼” diameter metal or
ceramic grinding balls with a conical-bottomed plastic tube, use the support
plug to help prevent failure of the tube. Hold the tube and support plug
between thumb and index nger. Press them together into the holder of the
Super FastPrep-2.
It is recommended that for best results, only Lysing Matrix Tubes supplied by
MP Biomedicals be used in the Super FastPrep-2.
If you use a 2 mm (or greater) diameter metal or ceramic ball in a plastic tube,
do not run the rotary tool above the speed setting of 25 (about 4,000 cycles per
minute). Doing so may cause failure.
If you use a tube with a tethered cap, be sure to insert the tube into the holder
with the plastic loop facing down to prevent it from damaging the clear window on
the lid to the Super FastPrep-2 during operation.
Tube
Support
Plug Tube
Holder

9
4. Close the lid and set the speed control on the rotary tool to the desired speed.
Below is a table of the approximate Super FastPrep-2 speed (+/- 1,000
cycles per minute) for each corresponding position of the speed control slide
on the Dremel® 8220 rotary tool, running with an empty tube.
5. While holding the lid closed with one hand, ip the on/off switch on the rotary
tool with the other hand to run the Super FastPrep-2 for the desired period
of time.
Caution: Do not operate the Super FastPrep-2 while the lid is open.
6. After turning off the rotary tool, open the lid and remove the 2mL tube.
Continue with downstream purication/isolation procedure.
Refer to corresponding purication kit instructions for details concerning sub
sequent isolation of DNA/RNA/Protein from lysate material. For a complete listing
of FastDNA, FastRNA, and FastProtein purication and isolation kits please visit
www.mpbio.com/sampleprep.
Cleaning the Super FastPrep-2
Make sure to disconnect the rotary tool from the Super FastPrep-2 before
cleaning. Wear protective gloves (latex/nitrile) during all cleaning procedures.
Clean the Super FastPrep-2 as necessary. The exterior of the Super FastPrep-2 can be
cleaned with a damp (water, dilute ethanol) paper towel or similar. Clean the lysis cham-
ber (exposed when lid is open) by wiping down the inner walls and tube holder. Allow all
components to air dry prior to use.
Should a lysis tube fracture or break in the sample chamber during
processing, stop processing and immediately clean it as recommended above.
Slide Cycles Per Minute
5 600
10 1,400
15 1,900
20 2,300
25 4,000
30 4,800

10
For Research Use Only
All of our products are intended for research use only. Not for use in diagnostic procedures.
Warranty
This product is guaranteed for one (1) year from the time of receipt. See below for repair/return
policy.
Returns
For repairs made under warranty, the Goods must be returned to MP Biomedicals intact (non-
tampered with, non-disassembled) with all components together and in their original packaging.
Goods may not be returned for credit except with Seller’s (i.e., MP Biomedicals) permission, and
then only in strict compliance with Seller’s return shipment instructions. Returned Goods (other
than defective products) must be returned freight prepaid by the Customer and will not be ac-
cepted without prior authorization of MP Biomedicals. The Goods must be returned in their original
packaging and in resalable condition. Any returned items may be subject to a 20% processing
(restocking) fee.
Terms of Purchase
We warrant to you, our direct customer, that our goods shall conform substantially to the descrip-
tion of such goods as provided in our catalogs and literature accompanying the goods until their
respective expiration dates. THIS WARRANTY IS EXCLUSIVE, AND WE MAKE NO OTHER
WARRANTY, EXPRESS OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANT-
ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE. Our warranty shall not be effective if
we determine, in our sole discretion, that you have altered or misused the goods or have failed to
use or store them in accordance with instructions furnished by us. Our sole and exclusive liability
and your exclusive remedy with respect to goods proved to our satisfaction (applying analytical
methods reasonably selected by us) to be defective or nonconforming shall be the replacement of
such goods free of charge, upon the return of such goods in accordance with our instructions, al-
though at our discretion we may provide a credit or refund. IN NO EVENT SHALL WE BE LIABLE
UNDER ANY LEGAL THEORY (INCLUDING BUT NOT LIMITED TO CONTRACT, NEGLIGENCE,
STRICT LIABILITY IN TORT OR WARRANTY OF ANY KIND) FOR ANY INDIRECT, SPECIAL,
INCIDENTAL, CONSEQUENTIAL OR EXEMPLARY DAMAGES (INCLUDING BUT NOT LIMITED
TO LOST PROFITS), EVEN IF WE HAD NOTICE OF THE POSSIBILITY OF SUCH DAMAGES.
If we manufacture custom goods for you based on instructions, specications, or other directions
you provide to us, we shall not be liable for the lack of sufciency, tness for purpose or quality of
the goods to the extent attributable to such instructions, specications, or other directions. We shall
not be liable for any loss, damage or penalty as a result of any delay in or failure to manufacture,
deliver or otherwise perform hereunder due to any cause beyond our reasonable control.
Patent/Trademark Disclaimer
Some technologies of the Super FastPrep-2 sample processor are patent pending. Unless stated
otherwise, no license or immunity under any patents shall be either granted or implied by the sale
of MP Biomedicals products. Dremel® is a trademark of Robert Bosch Tool Corporation.

11

Protocol Revision # 116012500-15OCT
Cat. No. 116012500
Worldwide Ordering and Technical Support
USA
Worldwide Headquarters
Tel: [phone]
Fax: [phone]
Europe
Phone: [phone]
Fax: [phone]
Australia
Tel: [phone]
Tel: [phone]
Fax: [phone]
Fax: [phone]
Belgium
Tel: [phone]
Fax: [phone]
Canada
Tel: [phone]
Fax: [phone]
China
Tel: [phone]
Fax: [phone]
France
Tel: [phone]
Fax: [phone]
Germany
Tel: [phone]
Fax: [phone]
India
Tel: [phone]
Fax: [phone]
Japan
Tel: [phone]
Tel: [phone]
Fax: [phone]
Latin America
Tel: [phone]
Fax: [phone]
The Netherlands
Tel: [phone]
Fax: [phone]
New Zealand
Tel: [phone]
Fax: [phone]
Poland
Tel: [phone]
Fax: [phone]
Russia
Tel: [phone]
Fax: [phone]
Serbia
Tel: [phone]
Fax: [phone]
Singapore
Tel: [phone]
Fax: [phone]
Switzerland
Tel: [phone]
Fax: [phone]
United Kingdom
Tel: [phone]
Fax: [phone]
mpbio.com/sampleprep
[email protected]
MP Biomedicals • 29525 Fountain Parkway • Solon, OH 44139 USA • tel: [phone] • fax: [phone]
Owners Manual