MSD MESO SECTOR S 600 User manual

MESO®SECTOR S 600
www.mesoscale.com®
USER MANUAL

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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Unauthorized Use of Trademarks or Service Marks
MESO SCALE DISCOVERY, MESO SCALE DIAGNOSTICS, MSD, MSD GOLD, DISCOVERY WORKBENCH, MULTI-ARRAY, MULTI-
SPOT, QUICKPLEX, SECTOR, SECTOR PR, SECTOR HTS, SULFO-TAG, R-PLEX, S-PLEX, U-PLEX, V-PLEX, STREPTAVIDIN GOLD,
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© 2013, 2017 Meso Scale Diagnostics, LLC. All rights reserved.
Manual Part Number: IM-MN-002-C
August 2017
Printed in USA

Table of Contents
1 Introduction..............................................................................................................................................................7
1.1 Intended Audience..........................................................................................................................................7
1.2 How to Use This Manual................................................................................................................................. 7
1.3 Warnings and Caution Symbols ......................................................................................................................8
1.4 Formatting Information................................................................................................................................... 8
2 Safety Information................................................................................................................................................... 10
2.1 Regulatory and Safety Certifications .............................................................................................................. 10
2.1.1 WEEE Compliance.......................................................................................................................... 10
2.1.2 Hazardous Substances ................................................................................................................... 11
2.2 Symbols and Labels ..................................................................................................................................... 11
2.3 General Operation ........................................................................................................................................ 13
2.4 Hazards ....................................................................................................................................................... 13
2.4.1 Electrical ....................................................................................................................................... 14
2.4.2 Chemical and Biological................................................................................................................. 15
2.4.3 Mechanical.................................................................................................................................... 16
2.4.4 ESD Sensitivity .............................................................................................................................. 17
2.4.5 Electromagnetic Interference and Susceptibility ............................................................................... 17
2.5 Software and Operating System Compatibility................................................................................................ 17
2.6 Decontamination Prior to Shipping or Servicing ............................................................................................ 18
3 System Description................................................................................................................................................. 20
3.1 Intended Use................................................................................................................................................ 20
3.2 Specifications Overview................................................................................................................................ 20
3.3 Plate Compatibility....................................................................................................................................... 20
3.4 System Components..................................................................................................................................... 20
3.5 The SECTOR S 600 Instrument...................................................................................................................... 21
3.5.1 CCD Camera and Telecentric Lens .................................................................................................. 22
3.5.2 Motion Control System .................................................................................................................. 22
3.5.3 Plate Barcode Readers.................................................................................................................... 22
3.5.4 MULTI-ARRAY Plate Input/Output Stacker....................................................................................... 23
3.5.5 Stack Tubes................................................................................................................................... 23
3.5.6 Single Plate Adapter....................................................................................................................... 25
3.5.7 Stacker Cover Plate........................................................................................................................ 25
3.5.8 Status LEDs................................................................................................................................... 26
3.5.9 Power Switch and Input/Output (I/O) Panel...................................................................................... 27
3.5.10 Halt Button (on some configurations).............................................................................................. 28
3.6 Microsoft Windows-Compatible Workstations................................................................................................ 29
3.7 MSD DISCOVERY WORKBENCH Software ..................................................................................................... 29
3.8 Operational Modes ....................................................................................................................................... 30
3.9 Image Readout ............................................................................................................................................. 30
3.10 MESO SECTOR Demonstration Plate ............................................................................................................. 31
3.11 Uninterruptible Power Supply ....................................................................................................................... 32
4 Installation ............................................................................................................................................................. 34
5 Quick Start ............................................................................................................................................................. 36
5.1 Start-up ....................................................................................................................................................... 37
5.2 Setup........................................................................................................................................................... 38

iv
5.3 Run ............................................................................................................................................................. 40
5.3.1 Operations..................................................................................................................................... 41
5.4 Results ........................................................................................................................................................ 42
6 Using the SECTOR S 600 ........................................................................................................................................ 44
6.1 Single Plate Run .......................................................................................................................................... 44
6.2 Stack Run—Multiple Plates.......................................................................................................................... 44
6.2.1 Loading the Stack Tube .................................................................................................................. 44
6.2.2 Unloading the Stack Tube............................................................................................................... 46
6.3 Robotics Integration ..................................................................................................................................... 46
7 Maintenance........................................................................................................................................................... 48
7.1 Preventive Maintenance................................................................................................................................ 48
7.2 Instrument Cleaning ..................................................................................................................................... 48
7.2.1 Adapter and Stack Tube Cleaning.................................................................................................... 49
7.3 Instrument Decontamination ......................................................................................................................... 49
8 Appendix ................................................................................................................................................................ 51
8.1 Troubleshooting Guide ................................................................................................................................. 51
8.2 Specifications .............................................................................................................................................. 54
8.2.1 SECTOR S 600............................................................................................................................... 54
8.2.2 Scientific Performance ................................................................................................................... 54
8.2.3 Environmental Specifications.......................................................................................................... 54
8.2.4 Power Requirements ...................................................................................................................... 54
8.2.5 SECTOR S 600 Physical Dimensions .............................................................................................. 55
8.2.6 Plate Standard Read Volume (per Well)........................................................................................... 55
8.2.7 Custom Bar Code Compatibility* .................................................................................................... 55
8.2.8 Plate Specifications........................................................................................................................ 55
9 Technical Support................................................................................................................................................... 57
9.1 Bug Reports and Suggestions ....................................................................................................................... 57
9.2 Problems Running SECTOR S 600 Instruments.............................................................................................. 57
9.3 How to Contact Us ....................................................................................................................................... 57

v
List of Figures
Figure 2:1 SECTOR S 600 electrical hazards.................................................................................................................... 14
Figure 3:1 SECTOR S 600 system ................................................................................................................................... 21
Figure 3:2 SECTOR S 600 components............................................................................................................................ 22
Figure 3:3 Custom Barcode Reader Selection Dialog........................................................................................................ 23
Figure 3:4 Input (right) stacker interface plate.................................................................................................................. 24
Figure 3:5 Fully loaded standard and high capacity stack tubes ........................................................................................ 24
Figure 3:6 SECTOR plate in single plate adapter .............................................................................................................. 25
Figure 3:7 SECTOR S 600 status LEDs ............................................................................................................................ 26
Figure 3:8 Power switch and I/O panel. ........................................................................................................................... 27
Figure 3:9 SECTOR S 600 halt button (on some configurations) ....................................................................................... 28
Figure 3:10 Cable attachment locations for SECTOR S 600............................................................................................... 29
Figure 3:11 The SECTOR S 600 reads 96-well plates in six sectors................................................................................... 30
Figure 3:12 MESO SECTOR Demonstration Plate ............................................................................................................. 31
Figure 3:13 Loading a demonstration plate...................................................................................................................... 32
Figure 5:1 Instrument Status window............................................................................................................................... 36
Figure 5:2 MSD DISCOVERY WORKBENCH desktop icon ................................................................................................. 37
Figure 5:3 SECTOR S 600 icon ....................................................................................................................................... 37
Figure 5:4: MSD DISCOVERY WORKBENCH splash screen............................................................................................... 38
Figure 5:5 SECTOR S 600 Window: Setup, Plate Summary, and Operations areas.............................................................. 39
Figure 5:6 Stacker cover over the output port with empty single plate adapter in the input port........................................... 39
Figure 5:7 MESO SECTOR demonstration plate in the single plate adapter ........................................................................ 40
Figure 5:8 SECTOR S 600 window, Run Options dialog box ............................................................................................. 41
Figure 5:9 Demonstration plate results, Color Map layer .................................................................................................. 42
Figure 6:1 Loading MULTI-ARRAY plates into stack tube ................................................................................................. 45
List of Tables
Table 2:1 Hazardous Substances..................................................................................................................................... 11
Table 2:2 Symbols and labels ......................................................................................................................................... 11
Table 3:1 Stack tube plate capacities............................................................................................................................... 23
Table 8:1 Troubleshooting guide..................................................................................................................................... 51
Table 8:2 Scientific performance ..................................................................................................................................... 54

6
1
Introduction

Introduction 7
1Introduction
MESO SCALE DISCOVERY (MSD) develops, manufactures, and markets biological assays that provide cost-effective and valuable
information to scientists in drug discovery, therapeutic screening, and life science research. MSD's product portfolio is based on MULTI-
ARRAY®technology, a proprietary combination of patterned arrays and electrochemiluminescence detection that results in exceptional
sensitivity, speed, dynamic range, and convenience.
MSD develops, manufactures, and markets detection instrument systems as well as a line of assay kits for use with these instruments, a
proprietary line of reagents, and custom microplate printing and assay development services. MSD®MULTI-ARRAY microplates are
available in 96- and 384-well formats with standard or high-binding surfaces. MSD plates may be purchased uncoated or coated with
proteins such as streptavidin or avidin, with anti-species antibodies such as goat anti-mouse or goat anti-rabbit, or with antibodies
against specific analytes. Custom coatings and surface treatments are available.
MSD MULTI-ARRAY plates are available as single spot (single assay) plates and as MULTI-SPOT®plates with patterned spot arrays in
each well. MULTI-SPOT plates measure multiple analytes simultaneously in a single well, increasing throughput and enabling novel
assay panels.
For more details and information about applications, please visit the MSD website at www.mesoscale.com.
1.1 Intended Audience
This manual is for all users of the MESO SECTOR®S 600 instrument. Users should understand general computer and Microsoft Windows
terminology, and be familiar with standard laboratory practices. The intended users of the SECTOR S 600 are those conducting research
in the life sciences.
This manual describes how to operate the SECTOR S 600 and acquire data using SECTOR MULTI-ARRAY and MULTI-SPOT plates. The
analysis of this data is treated in a separate manual: DISCOVERY WORKBENCH®User’s Guide.
1.2 How to Use This Manual
This manual is organized by chapters containing main topics and subsections. Use the hyperlinked Table of Contents to find topics of
interest quickly. The List of Figures and List of Tables hyperlink to the images and tables that enhance understanding of written
information in this manual. The Appendix contains supplemental information on troubleshooting, instrument specifications, safety
symbols, regulatory information, and labels.
Tips
Symbol 
Tips provide extra information or details that help users perform functions more efficiently.
Notes
Notes provide supplemental information on the proper use of the SECTOR S 600 and its software.

Introduction 8
1.3 Warnings and Caution Symbols
WARNING
General warnings advise operators of potential hazards and highlight the procedures or information necessary to avoid personal injury
during use of the SECTOR S 600.
Symbol
Explanation
Risk exists for a mechanical, chemical, or safety hazard
Risk exists for an electrical hazard
Risk of exposure to biohazards
CAUTION
A caution note highlights procedures or information necessary to avoid damage to equipment, corruption of software, loss of data, or
invalid test results.
CAUTION: Carefully read and understand all information in this document. Failure to read, understand, and follow the
instructions in this publication may result in damage to the product, injury to operating personnel or poor instrument
performance
1.4 Formatting Information
This guide uses the following formatting conventions:
Internal hyperlinks are formatted bold/gray. Click to jump instantly to the referenced section or figure.
External hyperlinks are formatted underlined/blue. Click to create an email message or open an external web page.
Clickstreams are indicated with arrows and always start with a top-level menu item: Select Tools Plate Data
History.
Information to be entered by the user is shown in italics: Enter Administrator
When referring to a term as it appears in the software, we capitalize it exactly as it is capitalized on-screen: Select
Read from Bar Code from the Plate Type menu.

9
2
Safety Information

Safety Information 10
2Safety Information
2.1 Regulatory and Safety Certifications
The SECTOR S 600 has been tested to comply with applicable regulatory standards; it carries the cSGSus mark and is CE marked.
Regarding EN 61326-1: 2013 Electrical Equipment for Measurement, Control, and Laboratory Use – EMC Requirements:
•The SECTOR S 600 instrument is designed for operation in a controlled electromagnetic environment. Transmitters of RF
energy such as mobile (cellular) telephones should not be used in close proximity.
Regarding FCC Rules, Part 15, Subpart B, a Class A digital device:
•This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC
rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated
in a commercial environment. The equipment generates, uses, and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause interference in which case the user will be required to correct the
interference at his own expense.
Regarding Industry Canada Interference-Causing Equipment Standard:
•This Class A digital apparatus complies with CAN ICES-001(A).
•Cet appareil numérique de la Classe A est conforme à la norme NMB-001(A) du Canada.
Contact MSD Scientific Support with inquiries about the regulatory compliance of MSD instrumentation.
2.1.1 WEEE Compliance
The MESO SECTOR S 600 was placed on the market after 2005 in compliance with European Union (EU) directive 2012/19/EU, the
Waste Electrical and Electronic Equipment (WEEE) Directive.
For all inquiries regarding recycling of shipping materials and instrument disposal, contact MSD Instrument Service.