MshOt MF43-N User manual

Guangzhou Micro-shot Technology Co., Ltd
MF43-N
Research grade fluorescence microscope
User Manual
Website：www.m-shot.com
Tel:[phone]
Email: [e-mail]

Research grade fluorescence microscope MF43-N

Thank you for buying our product!
This unit is a precision optical instrument. Though with high safety design, wrong usage and overlook of this manual
can do harm to you and your property. Thus, to ensure the life of this unit and maintain it properly, please read this
manual carefully before operating.
Safety Reminder
1. Be sure to turn off the power switch and remove the power cord before installing, replacing the
bulb or fuse, plugging and unplugging the power supply.
To prevent electric shock or fire, be sure to turn off the power switch and remove the power cord
before installing this unit, replacing the bulb or fuse, plugging and unplugging the power supply.
2. Do not disassemble
Except the removable parts mentioned herein, no part of this unit shall be removed, otherwise the
performance of this unit may be reduced, or may cause an electric shock, injury or damage to this
unit. Please contact the supplier if any fault occurs.
3. Input voltage
Check if the input voltage is consistent with your local voltage supply. If not, do not operate this
unit and contact the supplier. Improper input voltage may cause a short circuit or fire thereby
causes damage to this unit.
4. Use specific bulb, fuse and power cord
Use of an improper bulb, fuse or power cord may cause damage or fire to this unit. Any extended
power cord used must be grounded (PE).
5. Protect this unit from high temperatures, dampness and foreign objects
To prevent short circuit or any other fault, do not expose this unit to any high temperatures or
dampness environment for a prolonged period of time. A suitable operating environment is
designated at a temperature of 5°C-35°C, and relative humidity of 20%-80% (at 25°C). If water splashes on this unit,
turn off the power switch and remove the power cord immediately, and then wipe the water off with dry cloth. When any
foreign object enters or drips onto this unit, please stop operating the unit and contact the supplier.
6. Heat of light source
The lighting bulb generates high temperatures during operation. Do not touch the collector lens or
lamp box when the lamp is illuminated, and do not touch the bulb within 30 minutes after the
lamp goes out due to high temperatures arising from operation. When replacing the bulb, make sure it has cooled down
properly (the lamp should be off for at least 30min).
★To prevent burn, do not touch the bulb when the lamp is illuminated or within 30min after it goes out.
★To prevent fire, do not place any fibrous product, paper, flammable or explosive material (e.g., gasoline,
petroleum ether, alcohol) near the halogen lamp housing or mercury lamp housing.
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Warning!
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Warning!
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Warning!
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Warning!
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Warning!
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Warning!

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7. Coarse/fine focusing knobs
This unit employs a coarse/fine coaxial focusing mechanism. Do not turn the left/right coarse/fine
focusing knob in the opposite direction. When the objectives lifting device reaches the limit of
motion, do not continue to turn the coarse focusing knob, otherwise the focusing mechanism may be damaged.
8. Storage place
This unit is a precision optical instrument, and improper operation or storage may cause damage or
its precision may be adversely affected. Consider the following when selecting a storage place:
※Avoid placing the unit under direct sunlight, directly under interior lighting or any other bright place.
※A suitable operating environment is designated at a temperature of 5°C-35°C, and relative humidity of 20%-80% (at
25°C). Do not expose this unit to high temperatures, dampness or dust for a prolonged period of time, otherwise mist or
mold may develop or dust may deposit on the lens, thus cause damage to this unit and shortening its life.
9. Installation of bulb
Do not touch the glass surface of the bulb directly with bare hands. When mounting the bulb, wear
gloves or wrap it with cotton material.
※Wipe off any dirt on the surface of the bulb with a clean cotton fabric dipped in alcohol. If the dirt is not
thoroughly removed, it would etch the surface of the bulb weakening its brightness and shortening its life.
※Mount the bulb with care to avoid slipping off or injuries to your fingers.
※When replacing the bulb, make sure its contact is intact. If its contact is damaged, the bulb may be disabled or
short-circuited.
※When replacing the bulb, the feet should be inserted into the holder as deeply as possible. If the feet are not
tightly inserted, the bulb may go out or short circuit.
10. Instrument handling
This precision optical instrument is heavy and should be handled with care. Strong impact and
rough handling are strictly prohibited, it may cause damage to this unit.
11. Environmental protection
Please dispose the wastes from the packaging and operation of this unit by category such as
cartoon, foam, plastic, bulb and etc. Do not discard the damaged mercury lamp carelessly in order
to avoid creating environmental poll
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Warning!
Caution!
Caution!
Caution!

Content
Ⅰ. Components..................................................................................................................... 1
Ⅱ. Installation and operation.............................................................................................. 2
1. Baisc operation...........................................................................................................................................................3
2. Microscope operation................................................................................................................................................ 4
3. Adjust alignment........................................................................................................................................................5
4. Adjustable aperture diaphragm............................................................................................................................... 6
5. Focusing system......................................................................................................................................................... 7
6. Stage............................................................................................................................................................................ 8
7. Adjust the tension of the x-axis and y-axis knobs...................................................................................................8
8.Adjust the alignment of fluorescent light path.....................................................................................................11
Ⅲ. Installations steps...........................................................................................................9
1. Install LED lamp house.............................................................................................................................................9
2. Install objective nosepiece.......................................................................................................................................10
3. Install cable.............................................................................................................................................................10
4. Install condenser...................................................................................................................................................... 11
5. Install stage..............................................................................................................................................................11
6.Install gripper...........................................................................................................................................................11
7. Install fluorescence attachment..............................................................................................................................12
8. Install fluorescence light box.................................................................................................................................12
9.Fluorescent module installation cable...................................................................................................................12
10.Install eyepiece tube................................................................................................................................................13
11. Installing eyepiece...................................................................................................................................................13
12.Install adapter..........................................................................................................................................................13
13.Install the power cord.............................................................................................................................................14
Ⅳ、Broad spectrum LED light source..........................................................................15
Ⅴ、Troubleshooting guide................................................................................................ 17
Ⅵ、Technical specification................................................................................................ 23

1
Field diaphragm
Eyepiece
Trinocular tube
Fluorescence filter wheel
Host
Fluorescence illuminator lamp house
Fluorescence light barrier plate
X/Y stage hand wheel
Ⅰ.Components

2
Camera adapter（need to buy separately）
Focusing wheel
Light intensity rotate knob
Fluorescence illuminator
Objective
Stage
Bright field illuminator
power button

3
Ⅱ.Installation and operation
2-1、Basic operation
1. Turn on the power supply: turn on the power
supply and turn the main switch on the side of the
microscope to the "-" state.
2. Adjust the light intensity: turn the knob to
adjust the lighting intensity.
SET LIM(light intensity control）brightness
1、Turn off LIM switch ① (when LIM
indicator light ② is off) and turn the low
magnification objective into the light path to
focus the sample.
2、Turn the brightness adjustment knob ③
to select the best observation brightness. Press
the LIM setting button (set key) to input the
current brightness.
3、Repeat the above steps to set the best
brightness for each objective.
Each objective can be set a special brightness separately. After setting the brightness
for the objective lens, when the objective lens is selected, turn on LIM switch ①, and
the brightness will be automatically adjusted to the preset value; If it is necessary

4
to reset the best brightness, turn off LIM switch
① (when LIM indicator light ② goes out), turn
the objective lens that needs to reset the best
brightness into the optical path, turn off Lim
switch, and then repeat the above steps 3 and
4.Rotating the focusing knob to lower the stage.
3. Pull the slide holder apart and place the slide.
4. Rotate the objective holder to turn the 10x objective into the optical path（Adjust the
fluorescent accessory to neutral)
5. Rotate the coarse and fine focus system to focus the sample.
6. Moving the stage control hand wheel to adjust the observation sample area
2-2 Microscope adjustment
1. Adjust the pupil distance: adjust the binocular
lens tube to the left and right till the right field of
view is completely coincident.
Adjust the diopter: turn the diopter adjusting rings
on both sides to the scale "0", turn the 40x
objective into the optical path, observe with the
right eye from the right eye lens, and focus the
sample by rotating the coarse and fine focus
spiral.Turn the 10x objective into the optical path, and turn the right diopter adjusting ring