MshOt MF53-N User manual

Guangzhou Micro-shot Technology Co., Ltd
MF53-N
Inverted fluorescence microscope
User manual
Website：www.m-shot.com
Tel:[phone]
Email: [e-mail]

Inverted fluorescence microscope MF53-N

Thank you for buying our product!
This unit is a precision optical instrument. Though with high safety design, wrong usage and overlook of this manual
can do harm to you and your property. Thus, to ensure the life of this unit and maintain it properly, please read this
manual carefully before operating.
Safety Reminder
1. Be sure to turn off the power switch and remove the power cord before installing, replacing the
bulb or fuse, plugging and unplugging the power supply.
To prevent electric shock or fire, be sure to turn off the power switch and remove the power cord
before installing this unit, replacing the bulb or fuse, plugging and unplugging the power supply.
2. Do not disassemble
Except the removable parts mentioned herein, no part of this unit shall be removed, otherwise the
performance of this unit may be reduced, or may cause an electric shock, injury or damage to this
unit. Please contact the supplier if any fault occurs.
3. Input voltage
Check if the input voltage is consistent with your local voltage supply. If not, do not operate this
unit and contact the supplier. Improper input voltage may cause a short circuit or fire thereby
causes damage to this unit.
4. Use specific bulb, fuse and power cord
Use of an improper bulb, fuse or power cord may cause damage or fire to this unit. Any extended
power cord used must be grounded (PE).
5. Protect this unit from high temperatures, dampness and foreign objects
To prevent short circuit or any other fault, do not expose this unit to any high temperatures or
dampness environment for a prolonged period of time. A suitable operating environment is
designated at a temperature of 5°C-35°C, and relative humidity of 20%-80% (at 25°C). If water splashes on this unit,
turn off the power switch and remove the power cord immediately, and then wipe the water off with dry cloth. When any
foreign object enters or drips onto this unit, please stop operating the unit and contact the supplier.
6. Heat of light source
The lighting bulb generates high temperatures during operation. Do not touch the collector lens or
lamp box when the lamp is illuminated, and do not touch the bulb within 30 minutes after the
lamp goes out due to high temperatures arising from operation. When replacing the bulb, make sure it has cooled down
properly (the lamp should be off for at least 30min).
★To prevent burn, do not touch the bulb when the lamp is illuminated or within 30min after it goes out.
★To prevent fire, do not place any fibrous product, paper, flammable or explosive material (e.g., gasoline,
petroleum ether, alcohol) near the halogen lamp housing or mercury lamp housing.
7. Coarse/fine focusing knobs
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Warning!
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Warning!
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Warning!
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Warning!
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Warning!
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Warning!
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Warning!

This unit employs a coarse/fine coaxial focusing mechanism. Do not turn the left/right coarse/fine focusing knob in
the opposite direction. When the objectives lifting device reaches the limit of motion, do not continue to turn the
coarse focusing knob, otherwise the focusing mechanism may be damaged.
8. Storage place
This unit is a precision optical instrument, and improper operation or storage may cause damage or
its precision may be adversely affected. Consider the following when selecting a storage place:
※Avoid placing the unit under direct sunlight, directly under interior lighting or any other bright place.
※A suitable operating environment is designated at a temperature of 5°C-35°C, and relative humidity of 20%-80% (at
25°C). Do not expose this unit to high temperatures, dampness or dust for a prolonged period of time, otherwise mist or
mold may develop or dust may deposit on the lens, thus cause damage to this unit and shortening its life.
9. Installation of bulb
Do not touch the glass surface of the bulb directly with bare hands. When mounting the bulb, wear
gloves or wrap it with cotton material.
※Wipe off any dirt on the surface of the bulb with a clean cotton fabric dipped in alcohol. If the dirt is not
thoroughly removed, it would etch the surface of the bulb weakening its brightness and shortening its life.
※Mount the bulb with care to avoid slipping off or injuries to your fingers.
※When replacing the bulb, make sure its contact is intact. If its contact is damaged, the bulb may be disabled or
short-circuited.
※When replacing the bulb, the feet should be inserted into the holder as deeply as possible. If the feet are not
tightly inserted, the bulb may go out or short circuit.
10. Instrument handling
This precision optical instrument is heavy and should be handled with care. Strong impact and
rough handling are strictly prohibited, it may cause damage to this unit.
11. Environmental protection
Please dispose the wastes from the packaging and operation of this unit by category such as
cartoon, foam, plastic, bulb and etc. Do not discard the damaged mercury lamp carelessly in order
to avoid creating environmental poll
Caution!
Caution!
Caution!

Content
1. Components……………………………………………………………………………..1
2. Main observation steps………………………………………………………….....…..3
2-1、Bright field observation................................................................................................3
2-2、Phase contrat observation.............................................................................................5
2-3、Fluorescence observation..............................................................................................6
3. Installation and debugging of microscope……………………….......………………..8
4. Maintanence………...…………………………………………………………………12
5. Specification……………………………………………………………..….…………14
6. Troubleshooting……………………………………………………………………… 错
误！未定义书签。

1
1、Components
LED light source (bright field)
Phase contrast plate
Condenser
Stage
Eyepiece
Splitter lever
Stage control wheel
Focusing wheel
Coarse and fine focusing system

2
LED light control box（bright field）
12
Light barrier
Nosepiece
Broad spectrum LED lamp house
Fluorescence module
Camera port
Microscope body
Coarse and fine focusing wheel
Broad spectrum LED power control box（fluorescence）

3
2、Main observation steps
❶Turn on the bright field power supply / light intensity
adjustment switch knob (1), and the sound of "Ta"
indicates that it is on.
Adjust the light intensity: rotate the knob on the control box
of bright field power supply to adjust the light source from
"0" (off) to "100" (brightest), and adjust the light source to
the appropriate brightness.
❷Push the splitter lever ②forward and switch to the
eyepiece observation state.
The light path can be switched between eyepiece
observation and eyepiece / camera using the splitter lever.
❸Put the sample on stage ③.
❹Turn the objective nosepiece ④to turn the required
multiple objective into the light path.
①
Bright field observation
②
④
③

4
❺Adjust the aperture diaphragm adjusting rod ⑤to
change the intensity of light passing through the condenser.
The position of the aperture should correspond to the
magnification of the objective lens. For example, when
observing under the 10x objective lens, it is recommended
to adjust the adjusting rod of the aperture to one third to the right and one third to the left, so that the light
passing through is enough to ensure the best observation effect.
❻Remove the condenser barrier plate ⑥so that the
light can reach the sample through the condenser.
❼Turn the dial ⑦on the right side of the fluorescent
module to turn the neutral into the light path.
❽Adjust the focusing hand wheel ⑧to focus the
sample.
When the right hand wheel of coarse focus and fine
focus is rotated clockwise, the objective rises, otherwise
the objective falls. When the left coarse focus and fine
focus knobs are rotated clockwise, the objective
decreases, otherwise, the objective increases
⑥
⑦
⑧

5
❾On the basis of bright field observation, rotate the
objective nosepiece ④to turn the 10x phase contrast
objective into the optical path.
The shell of phase contrast objective has the word "pH"
❿Push the phase insert plate ⑤into the slot of the phase
insert plate (refer to the installation of the phase insert plate
in the installation procedure of the microscope), so that the
phase difference ring of the corresponding multiple of the
objective lens is in the optical path. Adjust the adjusting rod
of aperture diaphragm to the corresponding position.
Note: the multiple of the phase contrast ring should correspond to the multiple of the objective lens. If the
10x objective lens is used, the 10x hole should be selected for the phase contrast insert plate
⓫Remove the condenser barrier plate ⑥so that the light
can reach the sample through the condenser.
⓬Adjust the focusing hand wheel ⑧to focus the sample.
Phase contrast observation
④
⑥
⑧

6
⓭Push the condenser baffle plate into the light path.
In fluorescence observation, the baffle of the condenser can
reduce the stray light reflected by the condenser into the light
path and affect the fluorescence observation effect
⓮Gently press the word "play" on the left side of the
fluorescent power control box to turn on the fluorescent
light source, and adjust the fluorescent light intensity knob
to the appropriate brightness.
The left light intensity adjustment knob is the "UV" band
brightness adjustment knob, and the right light intensity
adjustment knob is the "B, G" band brightness adjustment knob. At this time, the bright field light source
should be turned off
⓯Turn the objective nosepiece ④to turn the required
multiple objective into the light path.
Note: do not use phase contrast objective lens when
fluorescent observation
⓰ Turn dial ⑦on the right side of the fluorescent
module to turn the desired fluorescence gear into the
optical path.
Fluorescence observation
⑥
⑦
④

7
⓱Adjust the focusing hand wheel ⑧to focus the sample.
The following procedure is applicable to all observation methods
⓲After adjustment, the coarse focusing limiting hand wheel
adjusting ring can prevent the object from colliding with the
sample. Lock the limit handwheel along the arrow "lock"
direction, that is, lock the upper limit of coarse focusing. The
device can also simplify focusing. After focusing the sample, lock
the limit handwheel, adjust the coarse focusing handwheel to the
limited position again to focus directly, and then use the fine
focusing handwheel to focus precisely
⓳Adjust the tension of the coarse focusing hand wheel
according to the user's needs. ⑩Turn the tension adjusting
ring of the coarse focusing hand wheel in the direction of the
arrow to increase the tension.On the contrary, reduce the
tension. If the objective slides down due to its own gravity, or
defocuses rapidly after focusing with the fine focusing
handwheel, it is caused by too small tension. It is necessary to turn the coarse focusing handwheel tension
adjusting ring along the direction of the arrow to increase the tension
Fluorescence
Mark
EX wavelength
（nm）
DM wavelength
（nm）
EM wavelength（nm）
UV
UV
330-380
400
420LP
Blue
B
460‒490
500
510LP
Green
G
510‒550
570
590LP
Neutral
/
/
/
/
⑧
⑨

8
⓴Adjust the pupil distance: when observing through the
eyepiece, adjust the binocular until the left and right fields of view
are completely consistent.
The indicator dot "." indicates the pupil spacing. Write down your
pupil spacing scale for next use
Adjust the diopter: after focusing the right lens tube clearly,
observe the left lens tube again. Adjust the diopter adjustment
ring on the left eyeglass tube ⑪until the image is clear in the
field of view.
3、Installation and debugging of microscope
❶Screw the objective on the objective nosepiece in order of
multiple size.
❷Place four square gaskets on the stage bracket.
The wider gasket is placed on the wide bracket, and the
narrower gasket is placed on the narrow bracket.
Do not cover the bottom screw hole.
⑪
❶
❷

9
❸Place the stage above the bracket gasket (keep the
screw holes aligned), and screw the hexagon screw into the
screw hole to fix the stage.
❹Place the drip plate over the stage.
❺Open the dust cover of the eyepiece tube and clip the
two eyepieces into the tube respectively.
❻Connect the bright field power cable on the
microscope bracket to the bright field light source control
box, and connect the adapter on the other end to power
on the microscope
❸
❹
❺
❻

10
❼Insert the phase contrast plate into the slot above the
condenser.
❽Remove one eyepiece and insert the centering
telescope into the eyepiece tube. The 10x objective is
turned into the optical path, and the phase insert plate is
pushed into the optical path corresponding to the 10x hole
position. Loosen the screw above the centering telescope,
pull out the lens properly, observe the imaging in the
centering telescope with one eye until two rings appear in
the field of vision, and then tighten the screw to fix the lens.
❾Use a hexagon key to adjust the left and right screws of
the left and right 10x holes, so that the bright ring and dark
ring in the field of view of the centering telescope become
concentric circles (a-b). After adjustment, take out the
centering telescope and replace it with eyepiece for
observation.Adjust the alignment of different multiples of
phase contrast objective respectively.
❼
❾
A
B

11
❿Clip the fluorescent baffle into the screw in front of
the microscope bracket. The fluorescent baffle can
protect the eyes of the observe.
⓫Clip the light box interface of wide spectrum LED light
source into the light box bayonet behind the microscope
bracket, and tighten the two screws on the side to fix
the light box. Note: when installing the light box, hold the
light box firmly and tighten the screws to ensure that the
light box is fixed to prevent the light box from falling.
⓬Pull out the plug of the power socket behind the
fluorescent power control box, and connect the wide
spectrum LED light source lamp box with the power
control box.
⓭Pull out the plug of the power socket behind the
fluorescent power control box, and connect the wide
spectrum LED light source lamp box with the power
control box.
❿
⓫
⓬
⓬
⓭

12
⓮Clip the fluorescent module into the module base
from the left side of the microscope, and slowly push it
along the base track to make the module clip into the
template base plate.
⓯Use a hexagon key to tighten the fixing screw on
the bottom plate of the module on the right side of
the microscope to fix the fluorescent module on the
bottom plate to avoid shaking.
⓰Loosen the side fixing screw, remove the dust
cover at the camera interface, clip the C-type CCD
adapter into the camera connection above the three
lens barrel, and tighten the side fixing screw.
Screw the camera / digital CCD into the thread above
the adapter and make sure the camera / digital CCD is
tight.
4、Maintenance
Cleaning of instruments
The surface of exposed lens shall not be touched by hand. The dust on it can be removed by soft brush
or gauze. If necessary, it can be wiped by using clean soft cloth, absorbent cotton lens paper, etc. dipped
Camera/Digital CCD
⓮
⓯
⓰