MshOt MF52-N User manual

MF52-N
Inverted Fluorescence Microscope
User Manual
Guangzhou Micro-shot Technology Co., Ltd
Website: www.m-shot.com
Email: [e-mail]

Inverted Fluorescence Microscope
MF52-N

Thank you for buying our product!
This unit is a precision optical instrument. Though with high safety design, wrong usage and overlook of this manual
can do harm to you and your property. Thus, to ensure the life of this unit and maintain it properly, please read this
manual carefully before operating.
Safety Reminder
1. Be sure to turn off the power switch and remove the power cord before installing, replacing the
bulb or fuse, plugging and unplugging the power supply.
To prevent electric shock or fire, be sure to turn off the power switch and remove the power cord
before installing this unit, replacing the bulb or fuse, plugging and unplugging the power supply.
2. Do not disassemble
Except the removable parts mentioned herein, no part of this unit shall be removed, otherwise the
performance of this unit may be reduced, or may cause an electric shock, injury or damage to this
unit. Please contact the supplier if any fault occurs.
3. Input voltage
Check if the input voltage is consistent with your local voltage supply. If not, do not operate this
unit and contact the supplier. Improper input voltage may cause a short circuit or fire thereby
causes damage to this unit.
4. Use specific bulb, fuse and power cord
Use of an improper bulb, fuse or power cord may cause damage or fire to this unit. Any extended
power cord used must be grounded (PE).
5. Protect this unit from high temperatures, dampness and foreign objects
To prevent short circuit or any other fault, do not expose this unit to any high temperatures or
dampness environment for a prolonged period of time. A suitable operating environment is
designated at a temperature of 5°C-35°C, and relative humidity of 20%-80% (at 25°C). If water splashes on this unit,
turn off the power switch and remove the power cord immediately, and then wipe the water off with dry cloth. When any
foreign object enters or drips onto this unit, please stop operating the unit and contact the supplier.
6. Heat of light source
The lighting bulb generates high temperatures during operation. Do not touch the collector lens or
lamp box when the lamp is illuminated, and do not touch the bulb within 30 minutes after the
lamp goes out due to high temperatures arising from operation. When replacing the bulb, make sure it has cooled down
properly (the lamp should be off for at least 30min).
★To prevent burn, do not touch the bulb when the lamp is illuminated or within 30min after it goes out.
★To prevent fire, do not place any fibrous product, paper, flammable or explosive material (e.g., gasoline,
petroleum ether, alcohol) near the halogen lamp housing or mercury lamp housing.
!
Warning!
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Warning!
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Warning!
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Warning!
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Warning!
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Warning!

7. Coarse/fine focusing knobs
This unit employs a coarse/fine coaxial focusing mechanism. Do not turn the left/right coarse/fine
focusing knob in the opposite direction. When the objectives lifting device reaches the limit of
motion, do not continue to turn the coarse focusing knob, otherwise the focusing mechanism may be damaged.
8. Storage place
This unit is a precision optical instrument, and improper operation or storage may cause damage or
its precision may be adversely affected. Consider the following when selecting a storage place:
※Avoid placing the unit under direct sunlight, directly under interior lighting or any other bright place.
※A suitable operating environment is designated at a temperature of 5°C-35°C, and relative humidity of 20%-80% (at
25°C). Do not expose this unit to high temperatures, dampness or dust for a prolonged period of time, otherwise mist or
mold may develop or dust may deposit on the lens, thus cause damage to this unit and shortening its life.
9. Installation of bulb
Do not touch the glass surface of the bulb directly with bare hands. When mounting the bulb, wear
gloves or wrap it with cotton material.
※Wipe off any dirt on the surface of the bulb with a clean cotton fabric dipped in alcohol. If the dirt is not
thoroughly removed, it would etch the surface of the bulb weakening its brightness and shortening its life.
※Mount the bulb with care to avoid slipping off or injuries to your fingers.
※When replacing the bulb, make sure its contact is intact. If its contact is damaged, the bulb may be disabled or
short-circuited.
※When replacing the bulb, the feet should be inserted into the holder as deeply as possible. If the feet are not
tightly inserted, the bulb may go out or short circuit.
10. Instrument handling
This precision optical instrument is heavy and should be handled with care. Strong impact and
rough handling are strictly prohibited, it may cause damage to this unit.
11. Environmental protection
Please dispose the wastes from the packaging and operation of this unit by category such as
cartoon, foam, plastic, bulb and etc. Do not discard the damaged mercury lamp carelessly in order
to avoid creating environmental poll
!
Warning!
Caution!
Caution!
Caution!

Content
I. Characteristics and application ......................................................................................................................................5
II. Structure and features....................................................................................................................................................1
III. Installation..................................................................................................................................................................... 1
IV. Technical specification................................................................................................... 错误！未定义书签。
V. Operation..........................................................................................................................................................................
错误！未定义书签。
●Bright field observation.......................................................................................................................4
● Phase contrast observation............................................................................................................ 8
● Fluorescence observation............................................................................................................... 9
●Operation of the photographic device.................................................................................................. 9
VI. Replace Bulb and Fuse............................................................................................................................................... 错
误！未定义书签。
VII.Maintenance................................................................................................................................................................ 错
误 ！ 未 定 义 书 签 。
VIII.Troubleshooting........................................................................................................................................................11

1
I. Characteristics and application
MF52 inverted fluorescence microscope consists of epi-fluorescence and inverted biological microscope system. It
adopts excellent infinity optical system with long WD plan field objective and WF eyepiece. Stable and compact body
along with high rigidness prevents it from shaking when observing. The rotary in-and-out condenser enables it to do
non-infected culture cell observation in high culture dish and cylindrical flask. The epi-fluorescence system adopts a
module design philosophy and can adjust the illumination system as well as switching fluorescence filter. The machine
can be applied in cell tissue and transparent liquid tissue observation, as well as bio-medical, medical detection and
disease prevention.
II. Structure and features
1. Eyepiece 2. Observation head 3. Host 4. Objective nosepiece 5. Coarse/fine focus 6. Objective 7. Stage
8. Insert plate condenser 9.Fluorescence attachment
Figure 01
1
7
6
4
2
8
5
3
9

2
III. Installation
1. representation
2.Installation steps and approach （figure 2）
(1) Remove the package. Take out body 1 and put it stably on the operation table. Take off related support and anti-ash
cover.
(2) Take out binocular eyepiece 5. Take off the anti-ash cover. Install it on the observation socket 6. Lock the head.
(3) Take off dust cover cap from eyepiece holder, put two eyepieces 4 into the holder and rotate to make them matched
well
(4) Unload the moving board and put the round glass stage board 2 in the middle of the stage if not needed.
(5) If the moving board is needed (default with installed from factory), select suitable culture dish board and put it in the
moving board frame.
(6) Connect power line to microscope body and check if all setup are safety.
figure 02
4
1
5
6
2
3
3
3
Make sure every component is clean and without any scratch.
Caution

3
(7) Power on
(8) Check all accessories and tools come together, well keep and store them.
IV. Technical specification
Eyepiece
Wide field SWF10X(field number:Φ22mm), high eyepoint, one diopter adjustable
Centering telescope
Observation tube
45˚ inclined, interpupillary distance 53~75mm, diopter adjustable
Infinity plan achromatic
objectives
Bright field
PLL 10X/0.25 Work distance:4.1mm
PLL 40X/0.45 Work distance:2.5 mm
Phase contrast objectives
PLL 10X/0.25 PHP Work distance:4.1 mm
PLL 20X/0.45 PHP Work distance:5.0 mm
Optional objectives
PLL 4X/0.12 Work distance:10.8mm
PLL 40X/0.58PHP Work distance:2.5mm
Epi-fluorescence illumination
system
12V/2A Cold LED light source, brightness continuously adjustable
3W LED lamp
Slider fluorescent filter group
UV filter group, excitation filter 330nm~380nm
Blue filter group, excitation wavelength 460nm~490nm
Green filter group, excitation wavelength 510nm~550nm
Phase contrast ring plate
10X, 20X and 40X in one unit
Focus system
Coaxial coarse/fine focus, with tension adjustable and up stop, minimum division of fine
focusing is 2μm.
Nosepiece
Quintuple nosepiece,ball bearing with anti fungus device
Stage
Fixed stage overall size is 227mmX208mm
Glass rotundity stage overall size is Ф118mm, inner size is Ф68mm
Mechanical moving device, moving range is 77mm (longitudinal)X135mm (transverse)
Culture dish holder 1
Inside locating slot size: 86mm (W)X129.5mm (L),
(suitable for circular culture dish Ф90mm)
Culture dish holder 2
Inside locating slot size: 34mm (W)X77.5mm (L),
(suitable for circular culture dish Ф68.5mm)
Culture dish holder 3
Inside locating slot size:57mm (W)X82mm (L),
(suitable for circular culture dish Ф60mm)
Culture dish holder 4
Inside locating slot size:29mm(W)X77.5mm (L),
(suitable for circular culture dish Ф35mm)
Transmitted illumination
system
9W white LED lamp with brightness adjustable
Push-pull type condenser, working distance 55mm
Camera adapter
Internal set 0.75X C-mount
Tool
Allen driver
Dust cover

4
V. Operation
● Bright field observation
1. Turn on lightening switch and adjust brightness
Turn on switch 1 (turn to “-”), adjust knob 2 to get suitable light for observation. (Figure 03)
2. Adjust condenser riser
Slider type condenser device, please put on phase contrast condenser 1 into the central place of light path, as figure 04
shows.
3.Reset diopter ring
Rotate the diopter ring 1, leave the FOV “0” align at the side mark line. (Figure05)
Check if the input voltage is consistent with your local
voltage supply. If not, do not operate this unit and
contact the supplier. Improper input voltage may cause
a short circuit or fire thereby causes damage to this
unit.
Figure 03
1
Figure 04
2
1
Long-term high brightness would do harm to the
life of the bulb.
Turn down the brightness when not used to
protect the unit.
Caution

5
1
2
a
b
Figure 05
4.Adjust Pupil Distance (PD)
This is for more comfortable and clearer observation. If the field appear two crossed circles as figure 05-a, rotate lens 2
to change the exit pupil center distance to reach a coincident circle as figure 05-b.
5. Place the specimen section or the dish
1) To observe the specimen on the slide, put the slide on the dish board directly. To observe culture tissue in the dish,
change the board according to the size of the dish.( Refer to the specification figure) See Figure 06.
Figure 06
1
2) Adjust the hand-wheel 1 and 2. The observation area should be right above the objective for convenient observation.
See figure 07.
6. coarse/fine focusing
(1) Use the 10X objective to focus
Rotate the nosepiece 1; put the 10 x objective in the light path. See figure 08.
(2) Rotate the coarse hand-wheel 3 or 6; raise the objective to the peak.
Observe with the eyepiece, lower the objective using the coarse hand-wheel
until the specimen image appears. See figure 08.
(3) Adjust hand-wheel 2, make the image clear. See figure 08.
(4) Lock the spacing hand-wheel 5 as figure 08 shows.
Figure 07
1
2

6
5）Coarse hand-wheel tightness adjustment: The coarse hand-wheel 3 or 6 has been preset to a suitable place. If
needed, use the hand-wheel 4 to adjust.
7.Adjust, load and unload stage moving board
(1) Stage moving ruler 1（Y) and（X）direction moving is controlled by hand wheel 2 and 3, as figure 09.
It will hard to work if adjust coarse focusing too much.
Notice
It will caused self protected status when moving stage ruler moved to its limitation,
components will rub against each other and lead the stage stop working.
Notice
Figure 09
1
2
3
Figure 08
1
2
3
4
5
6
Use the 10X objective to focus and set location. When change to higher magnification, use the
coarse hand-wheel to raise the objective to the location directly and then use the fine one to
adjust.
Caution
2

7
(2) Unload the moving board 1 when observe culture tissue in large dish. When unload, please put condenser system 4
out of the light path, take out the dish board and lower the objective. Unscrew the screw 5, place upside down on
working platform, it can not put sidelong, or it might be damaged . See figure 10.
(3) Tightness adjustment
It has been adjusted well from factory. If needed after long time using, unload the ruler, reverse it and do the steps
below.
(a) Y direction
Unscrew screw 4, unload the supporting board 3, unscrew the two screws 1 (a little bit) and then adjust screw 2 with
Allen wrench to adjust the tightness of Y transmission steel rope. See figure 11-a.
(b) X direction
Unscrew the two screws 5 and adjust screw 6 to adjust the tightness of X transmission steel rope. See figure 11-b.
Moving stage ruler is the key parts to hold slider and sample, it can not put
sidelong or without anything to support it on back after unload, please put it upside
down stable, or it be damaged on accuracy.
Notice
Figure 11
4
3
6
2
a
b
5
1
Figure 10
5
4

8
(4). Diopter regulation
Use the diopter ring 1 on the left lens to adjust diopter.
(a) Put the 40X objective in the light path, observe with right eye and
adjust till the specimen image is clear.
(b) Do the same step on left. The diopter range is N=±5. See figure
12.
● Phase contrast observation
1.Put specimen
Put the specimen on the stage board or directly on the stage, focus till the image clear.
2.centering
1)Take out one of the eyepiece, insert the eyepiece 2 into the lens as figure 13-a.
2)A dark ring 5 and a bright ring 6 would appear in the field as figure 13-c.
3)Adjust ring 1 if the edge of the dark or bright ring is not clear.
4)Adjust the plate as figure 13-c shows if the two rings not coincide with Allen wrench 3. Adjust screw 4 if it is a
pull-plate one. See figure 13-d.
5)After centering, take out centering eyepiece. And insert observation eyepiece again.
(a)
1
3
(b)
(c)
(d)
Figure 13
2
4
1
Figure 12
The objective should coincide with the plate in the condenser; otherwise the
effect of imaging would be worse.
Caution
Centering again when change magnification to ensure the imaging effect.
Caution
3
4
4

9
● Fluorescence observation
Modular designed LED epi fluorescence illuminating system takes use of LED as light source, it does not need to adjust
light central, just open to use. Before observe fluorescence, please close bright field illumination first.
1. Fix fluorescence attachment: push in the attachment to microscope bottom body and locked by screws.
2. Connect AC adapter to the attachment.
3. Choose needed excitation color light by slider, bright field (O), UV (U), blue (B), green (G).
4. Brightness control know can adjust LED lamp brightness.
Using tips:
1. Rotate brightness controller knob 2 and hear sound‘Ding’， the fluorescence light is open.
2. Default brightness is the most weak, knob 2 can control brightness.
According to sample observation needs, slider 1 is used to transfer between different filter groups, LED lamp changed
automatically.
● Operation of the photographic device
The push/pull rod is used to switch observation/camera. The camera output interface is
on the left of the body. Operation as below：
1. Unscrew screw 1 on the camera interface, take out the cover 2, see figure 15.
2. Set up the adaptor on the output interface, lock the screw. Turn on the camera
and make it work.
3. Put the 10X objective in the light path.
4. Push the rod 3, observe the specimen and adjust till the image clear.
4
1
3
2
Figure 14
2
Figure 15
1
3

10
5. Pull the rod 3; watch the monitor to check is the image is clear. If not, adjust fine knob.
6. For higher synchronization demanded, do below.
1) Push rod 3, observe specimen image. Find a target (a target easily to be recognized, as S in figure 16-a) and
remove it to the field center. Remove it to the cross point of the graduation eyepiece if you have as figure 16-b.
2) Pull rod 3, watch the image on the monitor and check if the target above is near the field center. If it is a long
distance, adjust the three screws on the output interface and remove the target to the center.
3) Move the specimen and check if the moving direction coincides with that of the monitor image. If not, adjust
the camera direction. Unscrew the screws on the interface, rotate the camera and make the two directions the same
and finally lock the screw.
VI. Replace Bulb and Fuse
This instrument fuse is used to light for transmitted lighting system, fuse 3 is integrated at the input power adapter of
host, as figure 17.
1. Replace transmission illumination halogen bulb
(1) Turn off the power switch 1, and unplug the power cord 2, as figure 17.
(2) Take out fuse basement 2 by flat screwdriver 1 as image shows, then take
off broken fuse and insert new fuse, out the fuse basement back to host input
power adapter.
(3) Re connect power to check if it works well.
2. Replace body fuse
The fuse is installed in the fuse socket 3, replace according the following
steps.
(1) Turn off the power switch 1 and unplug the power cord 2. See Fig. 29.
(2) Loose the fuse socket nut 2, remove the damaged fuse and replace a new
one. See Fig. 30.
(3) Connect the power cord and turn on the power switch to check whether the
a
b
Figure 16
Turn off the power switch and unplug the power cord before replacing the
bulb and the fuse, otherwise fire, personal injury or damage to this unit
may occur due to electric short circuit.
!
Warning!
Figure 17
1
2
3

11
fuse works.
VII. Maintenance
1. The power switch of the main unit is the power control. When finish using this unit, press the switch to “0” to cut off
the power supply, unless the electric component in this unit is still operating. When this unit is not to be used for a long
time, remove the power plug from the supply socket and keep all cables properly.
2. This unit should be kept clean. Remove any oil on the lens and clean the body with clean gauze (or silk fabric or
absorbent cotton) dipped with a little alcohol. Put on the dust shield until this unit is completely cool and dry.
3. Cleaning the lens surface
Blow off or wipe off any dust on the lens with a blower ball or a soft brush; heavy dirt and fingerprints can be removed
with lens tissue or soft cloth dipped with a little mixture of alcohol and ethyl ether gently (the mix ratio is: alcohol
20-30% and ethyl ether 70-80%).
4. Cleaning the surface of this unit: Wipe it with clean soft cloth; heavy dirt may be wiped off with a neutral detergent.
5. Keeping: When this unit is not to be used for a long time, turn off the power supply of this unit, allow the bulb to cool
down sufficiently, put on the dust shield, store this unit at a dry, ventilated and clean place free from any acid, alkali or
steam, otherwise mold may develop on the lens.
6. Periodic inspection: This unit should be inspected and maintained periodically to maintain its performance.
VIII.Troubleshooting
Fault
Cause
Disposition
Electronic
system
No light shown in the
field of view
The power switch is not turned
Turn on the power switch.
The fuse is damaged.
Replace the fuse.
The connector of the electric chassis
is in bad contact.
Check and have professional
repair it.
Optical and
imaging
device
Fluorescence attachment
can not be put into the
microscope slot, has
dark area in the field of
Attachment converter is not in the
right position
Recheck converter position
Objective, eyepiece or condenser is
Clean lens by alcohol-metabolic
or change new lenses
Wiping in direction shown on the right
is easier to clean.
Caution
WRONG
RIGHT
Caution!
Do not wipe this unit with any organic solvent (e.g., alcohol, ethyl ether or its
dilute solution), otherwise the surface paint of this unit may come off. It is
suggested that a layer of non-corrosive lubricant is applied on the moving
parts of this unit before the dust shield is put on, and place the eyepiece and
the objectives in a container with desiccant.

12
view or lighting is not
even, can not see full
field of view.
not clean
Nosepiece position is not right
Lift nosepiece to the highest
position
Attachment has not been set properly
Push the attachment stick the slot
surface
Slider loosen
Tighten slider draw in clockwise
Oil or dust is found in
the field of view.
There is oil or dust on the eyepiece
lens.
Wipe the eyepiece.
Defocusing or low
resolution
The objective is damaged.
Repair the objective (by a
professional).
There is oil or dust on the surface of
the lens of the
Wipe the objective or the
eyepiece.
The aperture of the aperture
diaphragm is too small.
Adjust the aperture of the aperture
diaphragm based on the objective
magnification (or numerical
aperture) used.
The objective deviates from the light
path.
Turn the nosepiece to the fixed
position.
Sample slider cover glass is too thick
or thickness
Prepare sample slider according
to objective parameters
The focal plane of the
image is inclined
(brighter on one side and
darker on the other)。
The lighting bulb is seriously inclined.
Reposition the lighting bulb.
The specimen is not laid flatly.
Lay the specimen flatly on the
object stage and hold it stably.
Surrounding is too bright
Create darker surrounding when
observing fluorescence
Phase contrast
performance is not good
enough
Phase contrast condenser is not
matched with phase contrast ring
Choose matched times phase
contrast condenser and phase
contrast ring
Phase contrast plate central is out of
phase contrast condenser light center
Adjust phase contrast center to
make two light circle overlay to
each other.
Sample is not suitable for phase
contrast observation
Change suitable sample
Mechanical
system
The image cannot
remain clear during
observation.
The focusing mechanism flows (slides
down) automatically.
Adjust the coarse adjusting hand
wheel.
The fine focusing mechanism fails
Check and have professional

13
repair it.
The stage loosens or is inclined.
Check and have professional
repair it.
Condenser lifting system
can not be set or not
accuracy
Set screw loosen
Re set screw
Lock device is too old
Check and have professional
repair it.
Mechanical stage
Y direction moving is too slightly and
leads X direction moving at the same
time.
Adjust Y direction driver wire
tightness
X direction moving is too slightly and
leads Y direction moving at the same
time.
Adjust X direction driver wire
tightness
Driver wire break
Change new driver wire requires
professional workshop to repair it.