Okolab UNO-COMBINED-CONTROLLER User manual

1
UNO-COMBINED-CONTROLLER
CO2Microscope Electric Top Stage Incubation System
Manual
Vers. 03.16

2
This page was left blank

3
Index
Index
1REVISION TABLE ............................................................................................................................................ 5
2PREFACE.......................................................................................................................................................... 6
3SYMBOLS DESCRIPTIONS............................................................................................................................ 7
3.1.1 Symbols in this manual and on the product:............................................................................7
3.1.2 Symbols on the product label: ............................................................................................7
4SAFETY NOTES............................................................................................................................................... 7
5AC SECTION WIRING DIAGRAM .................................................................................................................. 9
6UNO-COMBINED-CONTROLLER. DESCRIPTION. ................................................................................... 11
7EQUIPMENT AND CONNECTIONS ............................................................................................................. 12
Equipment Supplied.......................................................................................................... 12
Equipment Required (Not supplied)....................................................................................... 13
8UNO-COMBINED-CONTROLLER. OVERVIEW.......................................................................................... 13
Temperature Regulation .................................................................................................... 13
CO2Regulation ............................................................................................................... 13
Humidity Regulation.......................................................................................................... 14
9UNO-COMBINED-CONTROLLER. INSTALLATION AND USER GUIDE................................................. 15
Digital Temperature Controller connection ports. ....................................................................... 15
Tubing Connections.......................................................................................................... 16
Pressure Gauge set up and use ........................................................................................... 19
Assembling Instruction of the Bubbling Column......................................................................... 21
Cables connection............................................................................................................ 21
Incubation chamber.......................................................................................................... 24
Fine gauge thermocouple (Free Sensor-optional) ...................................................................... 27
10 CONTROL UNIT FRONT PANEL............................................................................................................ 28
11 TEMPERATURE CONTROL MECHANISM IN STANDARD CONFIGURATION ............................... 29
12 TEMPERATURE CONTROL MECHANISM IN CUSTOM CONFIGURATION AT 37.0°C ................. 31
13 UNIT OPERATION THROUGH PUSH BUTTON INTERFACE............................................................ 34
Main Page, Main Menu And Submenu Description..................................................................... 34
Button functions .............................................................................................................. 35
Main Page and Main Menu Navigation ................................................................................... 36
Changing Set Points......................................................................................................... 37
Selecting Offset Mode....................................................................................................... 37
13.5.1 Standard Offset Mode ................................................................................................ 37
13.5.2 Custom Offset Mode .................................................................................................. 40
Objective Heater Submenu................................................................................................. 41
Humidity Module Submenu................................................................................................. 44
Alarm Settings Submenu.................................................................................................... 45

4
Screen Settings Submenu .................................................................................................. 46
Product Information Submenu .......................................................................................... 47
Stop Mode.................................................................................................................. 47
UNO-TS software Active Message..................................................................................... 48
14 OBJECTIVE HEATER MANUAL CALIBRATION.................................................................................. 48
15 FLOW METER REGULATION................................................................................................................. 49
16 MAINTENANCE ........................................................................................................................................ 51
17 SUPPORT.................................................................................................................................................. 52
Web Conference For Assistance And Training.......................................................................... 52
Troubleshooting .............................................................................................................. 53
Technical Support............................................................................................................ 54
ItalyTechnical Specifications ............................................................................................... 54
18 FIGURES.................................................................................................................................................... 55

5
1Revision table
Revision Number
Additions or changes
Date
Editor
01
Edited
November 2014
Amelia Liccardo
02
Review
March 2015
Silvia Foppiano
Amelia Liccardo
03
Minor Review
June 2016
Francesco Marino

6
2Preface
UNO-COMBINED-CONTROLLER together with a stage top Electric chamber (preventing CO2
of the desired concentration from dissipating away from the sample) is a compact solution for
long term time lapse imaging. We will refer to UNO-COMBINED–CONTROLLER as ‘UNO’ from
here on. ‘UNO’ is designed to maintain all the required thermal and gas conditions for keeping
cells alive for the duration of the time lapse imaging session. “UNO”has a temperature control
range of 25-50°C. The system includes a Temperature Control Unit, a manual Gas Mixer -with
floating ball flow meters-, and a passive Humidity Module with its own heater. The UNO-
COMBINED-CONTROLLER can be connected to a stage top Electric Environmental Chamber
from here on referred to as ‘Incubator’. As OPTIONS a fine gauge thermocouple can be
added to monitor the temperature of a Reference Well located in close proximity to the
experimental one. An Objective Heater is REQUIRED when using oil immersion objectives.
Additionally, UNO-COMBINED-CONTROLLER can be equipped with an optional air pump: OKO-
AP, which is a plug and play solution for Air inlet and a convenient alternative to 100% Air
tanks/compressed Air lines. All the heated components of the ‘Incubator’are heated by 24V
DC and their temperatures monitored by High-Accuracy thermistors. Temperature sensors with
0.1°C accuracy are embedded into the following components: lid and base of the ‘Incubator’,
humidity module and objective-heating collar. A single thermal controller regulates the
temperature of the individual components above in a coordinated fashion avoiding
overshooting the target temperature. Each unit undergoes a careful factory calibration
guaranteeing that the sample temperature, inside the chamber, is kept at the desired value
within the range 25 –50 °C. The system is equipped with visual and sound alarm alert in case
the temperature limit is reached. The unit shows current and set point temperature in the
same display. ‘UNO’ includes an integrated manual CO2mixer allowing regulating Carbon
Dioxide Concentration in Air within the range 1 –20%. Before use please read this manual and
familiarize yourself with the functions and the operation of the device.

7
3Symbols Descriptions
3.1.1 Symbols in this manual and on the product:
The following symbols identify important information to note:
CAUTION or WARNING: This symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: This symbol warns you of circumstances or
practices that can affect the instrument’s functionality and must refer to
accompanying documents.
Tip Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully setup and use the instrument.
3.1.2 Symbols on the product label:
CE MARKING: This symbol indicates a product’s compliance with EU legislation.
PRODUCT DISPOSAL: This symbol indicates that this product must not be disposed
as urban solid waste.
This symbol indicates the protection degree against ingress of solids or liquids
inside the product.
4Safety Notes
Before operating the equipment please read carefully the instructions and the safety notes. If
you have any questions, please contact Okolab.
The equipment must only be used as intended and as described in this Manual.
Technically qualified personnel should only operate equipment.
Do not start up the equipment if some of its parts are damaged.
This instrument is not intended for use in locations where flammable or explosive
gases are present.
Handle the equipment with care.
Equipment and its internal parts can be damaged by dropping and by shock.
Do not use a volatile solvent such as paint thinner to clean the instrument.
Deformation or discoloration will occur. Use a soft, dry cloth to remove stains from
IP 30

8
the instrument. Not following these instructions can result in damage or breakdown
of the device and its accessories.
The products labels can be found on the rear panel of the Main Unit.
Do not exceed voltage indicated in this manual and on the product label.
Avoid excessive induction noise, static electricity, magnetic fields.
Do not expose this instrument to rain or moisture.
Prevent throttling and kinking of cables.
Some equipment parts may reach temperatures above 50°C. Please be careful
when handling it.
Check that the cables are well inserted into their connectors so they cannot slip off
This device is not intended for medical use
The display should not be rubbed with abrasive materials or hard objects as it may
be damaged.
Power cord of unit should be unplugged from electrical outlet when left unused for
long period of time.
VENTILATION, please make sure the vents on the unit are not blocked in any way
either partially or fully.
Unit should be situated away from heat sources such as radiators, heat registers,
stoves, or other appliances or processes that produce heat
Do not start up the equipment if the supply cable is damaged.
Connect the equipment only to grounded mains power socket.
Do not disconnect cables while in operation.
Do not open the unit. Do not remove cover or back.
Prevent metal fragments or lead wire scraps from falling inside instrument. This
may cause electric shock, fire or malfunction.
The unit does not contain user serviceable parts.
Before starting, assemble the equipment while unplugged from an outlet.
Unit should never be used where it can fall or be pushed into water.

9
International caution symbol marks this device. IMPORTANT: read the
“Safety Notes” before installing, using and commissioning this device, as
the notes contain important information relating to safety and EMC. Not
following these instructions can result in damage or breakdown of the
device and its accessories.
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, FINANCIAL OR OTHER LOSSES RESULTING
FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
5AC section wiring diagram
Figure 1 shows the alternating current (AC) section, designed to connect internal power
supplies (see Figure 1 label C) in parallel to low voltage power grid.
Power Connector, OM-BZ0310
Power supplies, TDK-Lambda-ZWS150-24 and ZWS5-5.

10
3
1
2L
N
PE
F1
F2
2.5A
2.5A
Power Connector
OM-BZ03105
1.5mm
BLUE
1.5mm
BROWN
24AWG BLACK
24V-DC
24V-DC
0V
0V
0V
0V
1.5mm BLUE
1.5mm BROWN
1mmq
BLUE
1mmq
BROWN
1.5mm
G/V
1mm
BLACK
1mm
RED
5V-DC
0V
Power Supply
TDK-Lambda
ZWS150-24
85 → 265V c.a.
24V c.c.
6.5 A
Power Supply
TDK-Lambda
ZWS5-5
85 → 265V c.a.
5V c.c.
1 A
POSITIVE
CLAMPS
24 V DC
NEGATIVE
CLAMPS
24V-DC
0V
0V
0V
1.5mm G/V
5V-DC
0V
0V
0V
M
24V-DC
0.5mm
BLACK 0.5mm
RED
BLUE
RED
0V
0V
24V-DC
0V
A
B B
Figure 1 AC section wiring diagram