Okolab H301-MINI Operation instructions

Okolab stage top incubator
H301-MINI tutorial
1

Heated & humidified 5%CO2
2
2) Open the Air and CO2regulators
(looking to the screen the regulators are on
the left wall at the end of the LSM700 row)
Down = open
Up = close AirCO2
1) Check the H2O level in the bottle
(if empty fill it with distilled H2O)

Objective heater 1/2
3
If an immersion objective will be used, mount around it the objective heater.
2) Select the objective that you will use
1) Remove the holder

Objective heater 2/2
4
Pay attention to do not entangle the cables
if you are switching to other objectives.
3) Bring the objective heater
from below the stage
(heated orange band+green
velcro tape)
4) Place the objective heater
around the objective
5) Tighten the velcro

Install the chamber 1/3
5
1) Install the multiwell plate holder
2) Place the chamber without the gas tube
(disconnect the tube if needed)

Install the chamber 2/3
6
3) Open the chamber removing the glass lid
(unscrew the two lateral screws)
4) Place the appropriate insert: 35mm dish or slide
Match the red dots

Install the chamber 3/3
7
5) Install an empty 35mm dish or a glass slide
(to empty the hole)
6) Close the chamber putting back the glass lid
7) Connect the gas tube
(pass the gas tube behind the condenser turret)

Switch ON the control unit
8
Switch on the device by pressing 5 sec
the power button
(all the components will start to heat)

Set the appropriate insert
9
Press:
1) Settings
2) Chamber & Insert
3) Insert icon
4) Select:
1x35-M single
(for 35mm dish)
or
1xGS-M single
(for a slide)
5) Press OK

Objective heater settings
10
Press:
1) Settings
2) Obj. heater
3) Enable or disable
4) Press OK
!!! Remember to disable the objective heater if not needed!!!

Install the sample
11
!!! The device should be pre-warmed at least two hours before usage!!!
1) Install the sample in the insert
2) In case you are using an immersion objective
add oil by removing the whole chamber

Switch OFF the control unit 1/2
12
1) Switch off the device by pressing 5 sec
the power button
2) Press OK
!!!If you press for less than 5 sec
only the display will turn off!!!

Switch OFF the control unit 2/2
13
AirCO2
3) Close the Air and CO2regulators
(looking to the screen the regulators are on
the left wall at the end of the LSM700 row)
Down = open
Up = close
4) Remove the sample
5) Disconnect the gas tube
6) Remove the chamber