Okolab LEO Operator's manual

QUICK INSTRUCTIONS
LEO
HAND HELD METER
REV05_DECEMBER 2022
PRELIMINARY OPERATIONS
TURN LEO ON
ORGANIZING DEVICES
Press and old the power button for 2 seconds.
Press on Settings > Device .
The warming up time
for gas measurement
is 15 minutes.
A full charge requires 4
hours. The recommended
rst charging time is 6 hours.
SET DATE & TIME Press on Settings > System > Date & Time .
incub 7
incub 1
incub 1

Connect the blue labeled end of TUBE-A to LEO gas inlet port
labeled with icon .
Put the cap on LEO gas inlet port labeled with icon .
Connect the red labeled end of TUBE-A to the incubator or the point
where measurement is required.
Use the tting tubes and/or additional tube in order to allow the
compatibility of the supplied tubing with the gas port of your
incubators.
If the incubator has the return input available, use the Output Tube
to connect the LEO gas outlet port labeled with icon to the
incubator gas recycle.
Press Pump/Diffusion Mode button to select the Pump
Mode.
Start a single point or logging gas measurement.
IN
1. CO2-O2 Measurement
ASPIRATION MODE
21
4
3
1
2
3
4
5
TUBE-A
IN
OUT
Connect the blue labeled end of TUBE-A to LEO gas inlet port labeled with icon .
Put the cap on LEO gas inlet port labeled with icon .
Connect the red labeled end of TUBE-A to the incubator or the point where measurement is required.
Use the tting tubes and/or additional tube in order to allow the compatibility of the supplied tubing with the gas port
of your incubators.
Do not exceed 300 mbarg upstream to LEO.
Press Pump/Diffusion Mode button to select the Diffusion Mode, and start a single point or logging gas
measurement.
IN
DIFFUSION MODE
1
2
1
2
3
4
TUBE-A
IN
FOR HUMID GAS
TUBE-BTUBE-A
If the sampling gas is humid, connect the red labeled end of TUBE-A and the
green connector of TUBE-B to the Moisture Trap.
Connect the end of Tube-B to the incubator or to the point where measurement
is required.
Press Dry/Wet Mode button to set the Wet Mode,
and insert the humidity and temperature values of your
incubator.
Before starting a Gas Logging, unscrew the condensation trap to make sure the
condensation trap top lid has been removed.
The condensation trap top lid
allows reducing the sampling gas
time during a Gas Single Point
measurement Gas Measurement
with Moisture Trap.
2 31
Always take care
of the room
ventilation.
6
Use the tting tubes and/or additional tube in order to allow the compatibility of
the supplied tubing with the gas port of your incubators.
Please verify the correct measurement mode for each incubator.

SINGLE POINT MEASUREMENT
LOGGING MEASUREMENT
DATA ANALYSIS
Press on CO2-O2 single point measurement icon .
Select for an automatic or for a
manual measurement.
: the CO2-O2 Single Point measurement
terminates automatically when the gas reading value
becomes stable.
: you have to click again on the CO2-O2 Single
Point Measurement Icon to stop the measurement.
CO2 and O2 gas concentration values appear on the
Homepage.
Press on CO2-O2 logging measurement icon .
Insert the measurement times using + and – :
Each: the time between measurements. It is the
acquisition frequency. The time can be inserted in
minutes and seconds.
For: is the logging function duration. Leo will perform
measurements and log them in the memory For the time
selected here.
Pump Time*: the time, in minutes, the pump is active and
aspirates gas, prior to saving a reading. This parameter is
available only if the Aspiration mode is selected.
Press .
CO2 and O2 gas concentration values appear on the
Homepage.
*The pump settings are not available when working in Diffusion Mode.
Use the supplied OTG Cable to download the stored data on a USB ash drive.
Press View icon > Download/Delete icon and select the data to download.
1
1
2
2
3
Auto
Start
Auto
Manual
Manual
5.0
50
Completed
6.0
60
%
mbar
%
mbar
5.0
50
Logging
9:11
6.0
60
%
mbar
%
mbar
Organize and analyze the downloaded data by using
the LEO DATA IMPORT Excel Macro.
Dowload available on oko-lab.com.

oko-lab.com OKOLAB S.R.L.
LANZAROOKOLAB.COM
OKOLAB USA INC.
PETRAKOKOLAB.COM
OKOLAB SHANGHAI CO., LTD.
TONGOKOLAB.CN
2. Calibration / CO2-O2 Zero Reset / CO2 Zero Reset
Connect the blue labeled end of TUBE-A to LEO gas inlet port labeled with icon
Put the cap on LEO gas inlet port labeled with icon .
Connect the clear connector of the Calibration Kit to the red labeled end of
TUBE-A.
Connect the yellow end of Calibration Kit to the Gas Cylinder*.
Use the tting tubes and/or additional tube in order to allow the compatibility of
the supplied tubing with the gas port of your gas cylinders.
Use the OUTPUT TUBE to connect a purge tube to LEO gas outlet port. Place the
purge tube in a well ventileted environment.
Set 1.0 barg on the pressure regulator of the pure N2 tank.
Do not exceed 1.5 barg.
Start the Calibration/CO2-O2 Zero Reset procedure (refer to LEO User Manual,
paragraph 13).
* Use N2 cylinder for CO2-O2 Zero Reset procedure.
Connect the blue labeled end of TUBE-A to LEO gas inlet port labeled with icon
Put the cap on LEO gas inlet port labeled with icon .
Connect the red labeled end of TUBE-A to the Calibration Gas Regulator*.
Use the tting tubes and/or additional tube in order to allow the compatibility of
the supplied tubing with the gas port of your gas cylinders.
Use the OUTPUT TUBE to connect a purge tube to LEO gas outlet port. Place the
purge tube in a well ventileted environment.
Open the Calibration Gas Regulator by using the valve.
Set the ow rate value at maximum 0.4 l/min.
Start the Calibration/CO2-O2 Zero Reset procedure (refer to LEO User Manual,
paragraph 13).
GAS CYLINDER WITH PRESSURE REGULATOR
CALIBRATION / CO2-O2 ZERO RESET
GAS CYLINDER WITH FLOW REGULATOR
1
1
2
2
5
4
1
1
2
2
3
3
4
4
5
5
TUBE-A
TUBE-A
3
CALIBRATION
KIT
6
6
7
* Use N2 cylinder for CO2-O2 Zero Reset procedure.
CO2 ZERO RESET
Connect the blue labeled end of SODA LIME KIT to LEO gas inlet port labeled with
icon .
Put the cap on LEO gas inlet port labeled with icon .
Start the CO2 Zero Reset procedure (refer to LEO User Manual, paragraph 13).
1
2
3
SODA LIME
KIT
2 1
Okolab suggests to perform the Zero
Reset procedure each month.
6
4
5
3
IN
IN
IN
IN
IN
IN
The CO2 Zero Reset procedure adjusts the zero of the CO2 sensor only.