Okolab LEO User manual

LEO
Handheld Analyzer for IVF Applications
Manual
IST1446_REV05
SV 3.1.64.0

This page was left blank

Index
1SYMBOL DESCRIPTION ..........................................................................................................................................................1
1.1 SYMBOLS USED IN THIS MANUAL AND ON THE PRODUCT ......................................................................................................................1
1.2 SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................1
2SAFETY NOTES..........................................................................................................................................................................2
3REGULATORY COMPLIANCE ................................................................................................................................................3
3.1 REGULATORY STATEMENT.........................................................................................................................................................................3
3.2 WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT DIRECTIVE (WEEE DIRECTIVE)..................................................................3
4PREFACE .....................................................................................................................................................................................4
4.1 SENSOR MODULES.......................................................................................................................................................................................5
5SUPPLIED EQUIPMENT..........................................................................................................................................................6
5.1 POWER SUPPLY............................................................................................................................................................................................7
5.2 SODA LIME KIT ............................................................................................................................................................................................8
5.3 MOISTURE TRAP..........................................................................................................................................................................................8
5.4 OTG-CABLE..................................................................................................................................................................................................9
5.5 FITTING TUBES ............................................................................................................................................................................................9
5.6 CALIBRATION KIT........................................................................................................................................................................................9
5.7 TUBE-A.......................................................................................................................................................................................................10
5.8 TUBE-B.......................................................................................................................................................................................................10
5.9 ADDITIONAL TUBES..................................................................................................................................................................................11
5.10 CO2-O2-MODULE (EMBEDDED IN LEO) .........................................................................................................................................11
5.11 PUMP (EMBEDDED IN LEO) ...................................................................................................................................................................11
6OPTIONAL PARTS................................................................................................................................................................. 13
6.1 T1-MODULE...........................................................................................................................................................................................13
6.2 T2-MODULE...........................................................................................................................................................................................13
7LEO CONNECTION PORTS .................................................................................................................................................. 14
8PRELIMINARY OPERATIONS............................................................................................................................................. 15
8.1 CHARGE THE BATTERY.............................................................................................................................................................................15
8.2 DATE &TIME SETTING ............................................................................................................................................................................16
8.3 MEMORY CHECK .......................................................................................................................................................................................16
9NAVIGATION MENU ............................................................................................................................................................. 17
9.1 HOMEPAGE.................................................................................................................................................................................................17
9.2 ALARM ICONS AND MEANINGS................................................................................................................................................................19
9.3 SETTINGS....................................................................................................................................................................................................19
9.3.1 Device...............................................................................................................................................................................................20
9.3.2 Calibration......................................................................................................................................................................................21
9.3.3 Pump.................................................................................................................................................................................................22
9.3.3.1 Pump Leak Test ................................................................................................................................................................................................ 22
9.3.4 Alarm ................................................................................................................................................................................................25
9.3.5 System..............................................................................................................................................................................................26
9.3.5.1 Date & Time........................................................................................................................................................................................................ 27
9.3.5.2 Power Saving..................................................................................................................................................................................................... 28
9.3.5.3 Memory ................................................................................................................................................................................................................29
9.3.5.4 Info .........................................................................................................................................................................................................................29
9.3.6 Display .............................................................................................................................................................................................30
9.3.6.1 Brightness ........................................................................................................................................................................................................... 31
9.3.6.2 Theme ...................................................................................................................................................................................................................31
9.3.6.3 Touchscreen .......................................................................................................................................................................................................32
9.3.7 Units..................................................................................................................................................................................................32
9.3.8 Factory Reset.................................................................................................................................................................................33
10 CO2-O2 MEASUREMENT PROCESS.............................................................................................................................. 34

10.1 CO2-O2 MEASUREMENT PROCESS:PRELIMINARY OPERATIONS....................................................................................................35
10.1.1 Diffusion Mode –Preliminary Operations .......................................................................................................................35
10.1.2 Aspiration Mode –Preliminary Operations ....................................................................................................................37
10.1.3 CO2-O2 Single Point Measurement.....................................................................................................................................39
10.1.4 CO2-O2 Logging ...........................................................................................................................................................................40
10.1.5 Wet Mode........................................................................................................................................................................................42
11 TEMPERATURE MEASUREMENT PROCESS.............................................................................................................. 43
11.1 TMODULES –PRELIMINARY OPERATIONS..........................................................................................................................................43
11.2 TEMPERATURE SINGLE POINT MEASUREMENT ..................................................................................................................................44
11.3 TEMPERATURE LOGGING.........................................................................................................................................................................44
12 READING DATA.................................................................................................................................................................. 47
12.1 VIEWING DATA..........................................................................................................................................................................................47
12.2 DOWNLOADING DATA..............................................................................................................................................................................49
12.3 DELETING DATA .......................................................................................................................................................................................54
13 CO2-O2-MODULE CALIBRATION ................................................................................................................................. 57
13.1 VIEW CURRENT.........................................................................................................................................................................................58
13.2 SPAN CALIBRATION..................................................................................................................................................................................58
13.2.1 CO2/O2 User Calibration using Span Gas Cylinder: Preliminary Operations ..................................................59
13.2.1.1 Span Gas Cylinder with Pressure Regulator......................................................................................................................................... 59
13.2.1.2 Span Gas Cylinder with Flow Regulator................................................................................................................................................. 60
13.2.2 CO2/O2 User Calibration with Certified Gas at atmospheric pressure: Preliminary Operations ...........61
13.2.3 Span Setting ...................................................................................................................................................................................62
13.2.4 Start...................................................................................................................................................................................................64
13.2.4.1 Auto Gas calibration.......................................................................................................................................................................................64
13.2.4.2 Manual Gas Calibration ................................................................................................................................................................................65
13.3 FACTORY ....................................................................................................................................................................................................66
13.4 CO2 ZERO RESET .....................................................................................................................................................................................67
13.4.1 CO2 Zero Reset with Air...........................................................................................................................................................68
13.4.2 CO2 Zero Reset with N2 ...........................................................................................................................................................70
13.5 OFFSET .......................................................................................................................................................................................................74
14 T1 AND T2 MODULES CALIBRATION......................................................................................................................... 77
14.1 VIEW CALIBRATION..................................................................................................................................................................................77
14.2 START MULTI-POINT CALIBRATION .....................................................................................................................................................78
14.3 FACTORY CALIBRATION...........................................................................................................................................................................79
15 TOUCHSCREEN CALIBRATION ..................................................................................................................................... 81
16 CLEANING & MAINTENANCE......................................................................................................................................... 82
17 CO2-O2-MODULE EXTRACTION................................................................................................................................... 83
18 PUMP EXTRACTION ......................................................................................................................................................... 85
19 SUPPORT ............................................................................................................................................................................. 89
20 TROUBLESHOOTING........................................................................................................................................................ 90
21 TECHNICAL SPECIFICATION ......................................................................................................................................... 91
21.1 LEO.............................................................................................................................................................................................................91
21.2 CO2-O2-MODULE ................................................................................................................................................................................92
21.3 T1-MODULE...........................................................................................................................................................................................93
21.4 T2-MODULE...........................................................................................................................................................................................93
22 FIGURE LIST........................................................................................................................................................................ 94
MANUAL REVISION TABLE ........................................................................................................................................................ 96

1
1Symbol description
This paragraph describes the symbols used in this manual and on the product label.
1.1 Symbols used in this manual and on the product
The following symbols identify important information:
CAUTION or WARNING or IMPORTANT: This symbol warns of circumstances or practices that
can affect the instrument’s functionality. Please refer to accompanying documents.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
1.2 Symbols on the product label
CE MARKING: This symbol indicates the product’s compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.
This symbol indicates the protection degree against ingress of solids or liquids inside the product.
IP 40

2
2Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−This equipment must be used only as intended and described in this Manual.
−This equipment should be operated only by qualified personnel.
−Do not use this equipment if any parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Always transport equipment with care.
−The equipment and its internal parts may be damaged by dropping or shock.
−Do not use a volatile solvent, such as paint thinner, to clean the instrument, deformation or
discoloration will occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Not following these instructions could damage the device and its accessories.
−Do not exceed the voltages indicated in this manual or on the product label.
−Avoid excessive induction noise, static electricity, or magnetic fields.
−Do not expose this instrument to water or moisture.
−Avoid throttling and kinking of tubes.
−Check tubing from time to time for possible wear and tear.
−Check that all tubing is fully inserted into connectors, and secure.
−This device is not designed for use in medical applications.
International caution symbol marks this device. It is important to read the “Safety Notes” before
installing, using and commissioning this device, as the notes contain important information relating
to safety and EMC. Not following these instructions can result in damage or breakdown of the device
and its accessories.
We reserve the right to make technical upgrades and changes.
IN NO EVENT, SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL, OR CONSEQUENTIAL
DAMAGES OF ANY NATURE –INCLUDING: LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT,
OR THE USE OF ANY PRODUCT.

3
3Regulatory Compliance
3.1 Regulatory Statement
The product complies with the essential requirements of the LVD Directive 2014/35/EU and the EMC
Directive 2014/30/EU. The product has been tested and it complies with the requirements of the following
standards:
−European standard EN 61010-1: 2010 “Safety requirements for electrical equipment for
measurement, control and laboratory use”
−European standard EN 61326-1: 2013 “Electrical equipment for measurement, control and
laboratory use –EMC requirements”
The product meets the technical requirement of the Directive 2011/65/EU Restriction of the use of certain
hazardous substances (RoHS). The product has been tested according to European Standard EN 50581: 2012
“Technical documentation for the assessment of the electrical and electronic products with respect to the
restriction of hazardous substances”.
3.2 Waste Electrical and Electronic Equipment Directive (WEEE Directive)
The product must not be disposed as a general household waste. At the end of the product’s life, take the
product to a collection point designed for recovering and recycling of electrical and electronic devices.
In case of doubt, please return the product to Okolab s.r.l. for proper treatment.

4
4Preface
LEO (see Figure 1 for front and rear view of LEO) is a handheld meter for IVF Applications, allowing
measurement of CO2-O2 gas composition, temperature, relative humidity, gas flow of the high-top and bench-top
incubators, by using multiple sensor modules.
The CO2-O2-MODULE is embedded into LEO, and it is included standard. It measures, with a high accuracy,
the CO2-O2 volume concentration in the unit of %, and CO2-O2 partial pressure in the units of mbar, psi and KPa.
Other (external) sensor modules that can be connected to LEO’s connection ports are: T1-MODULE, T2-
MODULE, CO2-PPM-MODULE, HUMIDITY-MODULE, FLOW-RATE-MODULE. All sensor modules are calibrated by
using traceable standards, and store the calibration data in the internal memory. The sensor modules can be
delivered with traceable or accredited calibration certificate, and sent to Okolab for re-calibration. Moreover,
sensor calibration can be performed by using LEO intuitive user calibration routines.
LEO works in Diffusion Mode when the gas to be sampled is pressurized and in Aspiration Mode when it
works at atmosphere pressure, sucking the gas from the incubator by an internal pump. In both cases, if the gas
inside the incubator is humid, prior de-humidification with the Moisture Trap (included standard with LEO) is
needed to retain water molecules.
LEO can improve the CO2 and O2 reading accounting the water content of your incubator by use Wet
Mode. The temperature and relative humidity (RH) values of your incubator must be entered.
LEO can perform both single measurement and extended time logging, storing the data in its local memory.
LEO allows organization of the stored data, associating a label to each device. You can store up to 40 devices labels.
The stored data to be downloaded through the micro USB port, and analyzed by using the free LEO DATA IMPORT
Excel Macro.
LEO features a user-friendly 4.3” touch screen interface.
a b
Figure 1. LEO: front (a) and rear (b) view.

5
4.1 Sensor Modules
The available Sensor Modules (see paragraph 10 and paragraph 13.4)
−CO2-O2-MODULE (embedded into LEO)
−T1-MODULE (external)
−T2-MODULE (external)
−CO2-PPM-MODULE (external)
−HUMIDITY-MODULE (external)
−FLOW-RATE-MODULE (external)
Figure 2. LEO with T1-MODULE and T2-MODULE

6
5Supplied equipment
Figure 3 shows the Hard Case containing LEO’s equipment. The optional and spare parts can be purchased
from Okolab s.r.l. Please see www.oko-lab.com or contact [e-mail] for more details.
A
B
C
D
G
H
F
E
I
J
K
L
M
Figure 3. LEO Hard Case.
A LEO. Hand-held meter for IVF applications.
B Power Supply (see paragraph 5.1).
C USB Cable.
D Soda Lime Kit (see paragraph 5.2).
E Moisture Trap, for humid gas measurement (see paragraph 5.3).
F Filter
G OTG Cable (see paragraph 5.4).
H Lanyard.
I Fitting Tubes (see paragraph 5.5).
J Tube-B (see paragraph 5.8).
K Tube-A (see paragraph 5.7).

7
L Calibration Kit (see paragraph 5.6).
M Additional tubes
5.1 Power Supply
LEO is powered by a rechargeable battery. A power supply is included with the device for charging the
battery from a power outlet. Connect the USB cable between the LEO MICRO-USB port and the power adapter to
charge the internal battery in LEO (see Figure 4).
MICRO-USB
PORT USB CABLE POWER ADAPTER
Connect to a live
electrical outlet
Figure 4. LEO connection for battery recharging.
Figure 5. Power Adapters supplied.

8
Do not use a different power supply to recharge LEO. Do not attach a different USB cable to the
power adapter.
5.2 Soda Lime Kit
The Soda Lime Kit (D in Figure 3) is used for the CO2 Zero Reset procedure with ambient air (see paragraph
13.4.1). It consists of a Soda Lime filter (1 in Figure 6) generally used to remove CO2 from the ambient air.
The Soda Lime Kit must be connected to Tube-A to start the CO2 Zero Reset procedure using ambient air.
The clear connector (2 in Figure 6) of Soda Lime Kit has to be connected to the red connector of Tube-A (1 in
Figure 10).
Note ►The Soda Lime kit has to be replaced when the Soda Lime filter (1 in Figure 6) changes color turning
purple. Contact Okolab to purchase a new Soda Lime Kit.
Note ►Remove the protection cap from the Soda Lime filter before using the Soda Lime Kit.
Figure 6. Soda Lime Kit.
5.3 Moisture Trap
The Moisture Trap (E in Figure 3) is used for humid gas measurements.
Figure 7 shows the Moisture Trap components:
1. A condensation trap allowing for the reduction of sampled gas temperature and the collection of
condensed water from the sampled gas (4 in Figure 7). The condensation trap top (3 in Figure 7)
allowing reducing the sampling gas time during the Single Point measurement must be removed when
Gas Logging occurs.
2. A Selemion Tube that reduces the relative humidity of sampled gases to a value lower than 40% (5 in
Figure 7).
The connector marked in green is the Gas Inlet port of the Moisture Trap (1 in Figure 7). Please use
Tube-B to connect the sampling Gas Port of the incubator to Gas Inlet port of the Moisture Trap.
The connector marked in red is the Gas Outlet port for the Moisture Trap (2 in Figure 7). Please use
the Tube-A to connect the Gas Outlet port of the Moisture Trap to the Gas Inlet port of LEO (see Configuration
b in Figure 46 for the measurements in Diffusion Mode or Configuration b in Figure 48 for the measurements
in Aspiration Mode).
1 2

9
FRONT BA CK
34 21 5
Figure 7. Moisture Trap.
5.4 OTG-Cable
The OTG Cable (G in Figure 3) can be used to download data stored on LEO to a USB Key. USB Key is not
supplied.
Figure 8. OTG Cable
5.5 Fitting Tubes
Okolab supplies 5 different types of reduction fitting tubes (I in Figure 3) allowing the compatibility of its
tubes with many sampling gas ports of the incubators.
Okolab provides the following reduction fitting tubes:
1. from ID=1.6mm to ID=3.2mm
2. from ID=2.4mm to ID=3.2mm
3. from ID=4.0mm to ID=3.2mm
4. from ID=4.8mm to ID=3.2mm
5. from ID=6.4mm to ID=3.2mm
Three additional caps for LEO’s ports, a male and female luer connector have been also supplied.
Note ►Additional caps are used to perform the Leak test of the pump (see paragraph 9.3.3.1).
5.6 Calibration Kit
The Calibration Kit (L in Figure 3) allows calibration of CO2-O2-MODULE.
Figure 9 shows the Calibration Kit. The clear connector (1 in Figure 9) has to be connected to red
connector of TUBE-A (1 in Figure 10). The end of Calibration Kit (3 in Figure 9) has to be connected to the gas

10
cylinder. The calibrated orifice (2 in Figure 9) guarantees a gas flow rate of 150 ml/min when the output pressure
from the gas cylinder is set at 1.0 barg (14.5 psig).
The relief valve (4 in Figure 9) allows avoiding over pressurization.
For more details on the connections and the use of the Calibration Kit, see the paragraph 11.
Note ►Do not exceed 1.5 barg (22 psig) during the calibration procedure (see paragraph 11).
Note ►Remove the protection caps from Calibration Kit before using it.
1 32
4
Figure 9. Calibration Kit
5.7 Tube-A
Tube-A (K in Figure 3) is used to connect the Gas Inlet connector of LEO to the Tube-B if the gas to be
sampled is dry or to the Gas Outlet connector of the Moisture Trap, if the gas is humid.
Figure 10 shows Tube-A. Connect the red connector (1 in Figure 10) to the green end of Tube-B (1 in
Figure 11) when sampling a dry gas or to the Gas Outlet connector (labeled green) of the Moisture Trap (1 Figure
7) when sampling a humid gas. The clear connector of Tube-A (3 in Figure 10) must be connected to one of the
Gas Inlet connector of LEO (see Figure 46 for the measurement in Diffusion Mode or Figure 48 for the
measurements in Aspiration mode). A hydrophobic filter (2 in Figure 10) is fitted in order to avoid water collection
at the Gas Inlet.
1
23
Figure 10. Tube-A.
Note ►Okolab suggests to replace the filter every six months.
Note ►Remove the protection caps from Tube-A before using it.
5.8 Tube-B
Tube-B (J in Figure 3) is used to connect the sampling Gas Port to the Tube-A if the sample gas is dry, or
to the Gas Inlet connector of the Moisture Trap if the sample gas is humid.
Figure 11 shows Tube-B. The green connector (1 in Figure 11) must be connected to the red connector
of TUBE-A (1 in Figure 10) when sampling the dry gas or to the Gas Inlet (labeled green) connector of the Moisture

11
Trap (1 in Figure 7). The end of Tube-B (2 in Figure 11) must be connected to the sampling Gas Port of the
incubator.
2
1
Figure 11. Tube B
Note ►Remove the protection caps from Tube-B before using it.
5.9 Additional tubes
Okolab supplies the following additional tubes (M in Figure 3):
1. Tygon tube (OD = 6mm) with a length of 100 cm
2. Tygon tube (OD = 4 mm) with a length of 30 cm
3. Silicone tube (OD = 4mm) with a length of 10 cm
5.10 CO2-O2-MODULE (embedded in LEO)
The CO2-O2-MODULE is embedded into LEO, and it is included standard. It measures, with a high accuracy,
the CO2-O2 volume concentration in the unit of %, and CO2-O2 partial pressure in the units of mbar, psi and KPa.
CO2-O2-MODULE is temperature and pressure compensated thanks to a dedicated temperature and pressure
sensor that are included in this module.
The CO2-O2-MODULE is factory calibrated by using traceable standards, and stores the calibration data
in its internal memory. The declared accuracy is guaranteed for a period of 6 months from the factory calibration
date. The CO2-O2-MODULE is designed for an easy extraction from LEO (see paragraph 14).
The CO2-O2-MODULE lifetime is 5 years.
Figure 12. CO2-O2-MODULE
5.11 Pump (embedded in LEO)
The pump is a micro diaphragm pump that guarantee the desired gas flow rate (from 60 ml/min to 260
ml/min). The default flow rate is 150 ml/min. The pump is designed for easy extraction from LEO, see paragraph
18). Figure 13 shows an image of the pump inside LEO.

12
Figure 13. Pump

13
6Optional Parts
1. T1-MODULE
2. T2-MODULE
6.1 T1-MODULE
The T1-Module (see Figure 14) is a calibrated temperature probe that can be read and displayed by LEO.
The T1-MODULE has an external diameter of 0.13 mm and it’s suitable for temperature measurements of benchtop
and hightop incubators where the temperature monitoring port has a diameter smaller than 2mm. T1-MODULE
stores calibration data in its own local memory
Tip ►Calibration data is available in the Calibration page of LEO when T1-MODULE is connected to LEO
(see paragraph 12).
Figure 14. T1-MODULE
Note ►Okolab recommends that the T1-MODULE should be calibrated after one year of use.
6.2 T2-MODULE
The T2-MODULE (see Figure 15) is a calibrated temperature probe that can be read and displayed by LEO.
The T2-MODULE has an external diameter of 2 mm and it’s suitable for temperature measurements of benchtop
and hightop incubators where the temperature monitoring port has a diameter bigger than 2 mm. T2-MODULE
stores calibration data in its own local memory.
Tip ►Calibration data is available in the Calibration page of LEO when T2-MODULE is connected to LEO
(see paragraph 12).
Figure 15. T2-MODULE
Note ►Okolab recommends that the T2-MODULE should be calibrated after one year of use.

14
7LEO connection ports
All connection ports of LEO are illustrated in Figure 16.
Figure 16. LEO connections
LEO features:
1. Power button
There are many different press modes:
-To turn LEO on: press and hold the power button for 2 seconds.
-To turn LEO off: press and hold for 2 seconds the power button, and tap OK to confirm.
-Press and hold for 5 seconds the power button to start the Touchscreen Calibration procedure (see
paragraph 15).
-Press and hold for 10 seconds the power button to restart LEO.
-Press the power button to lock and unlock LEO’s screen during the use.
-Press the power button to display the battery charging status (see paragraph 8.1).
2. MICRO-USB port. To charge the battery with the power adapter (see paragraph 5.1) and to download
software updates
3. Gas input port labeled with icon .
4. Gas input port labeled with icon
5. Probe connector 1. To connect additional external sensor modules
6. Probe connector 2. To connect additional external sensor modules
7. Gas outlet for gas release during the gas sampling measurement
1
2
73
65
4

15
8Preliminary operations
8.1 Charge the battery
To charge the battery, connect the power adapter to the MICRO-USB port (2 in Figure 16). If the battery is
being charged while LEO is turned on, the Charging symbol appears at the top of the LEO display (1 in Figure
17). The percentage of battery charge shows besides the battery symbol (2 in Figure 17). When charging is
complete, the Battery full symbol appears at the top of display.
v
1
2
Figure 17. Homepage - battery
If LEO is switched off, press the power button to display the battery charging status and the percentage of
battery charge (see Figure 18).

16
Figure 18. Battery charging status.
Note ►If you charge LEO with computer, the screen in Figure 18 a appears that means that LEO is not
getting the proper charging power that it expects.
Note ►A full charge requires 4 hours. The recommended initial charging time is 6 hours.
Okolab recommends not to use LEO during the first charging.
Note ►Okolab suggests to use LEO while charging the battery only if the percentage of battery charge is
higher than 90%.
8.2 Date & Time setting
When turning LEO on for the first time, Date & Time have to be set because the current date & time values
are stored with every reading. To change Date & Time and their format see paragraph 9.3.5.1.
8.3 Memory Check
When you want to use LEO for a large number of measures, Okolab recommends checking the system
memory. LEO has a memory page where the stored records and the still available records are shown (see
paragraph 9.3.5.3).
In the System Memory 300 records can be stored. The memory page shows how many records can be
saved. To check memory status, follow the indications described in the paragraph 9.3.5.3.
a b