Olink Signature Q100 User manual

®
Olink® Signature Q100
User Manual
1172 v1.1, 2022-01-25

Table of contents
1. About This User Manual............................................................................................................3
1.1 Safety Alert Conventions.................................................................................................3
1.2 Safety Data Sheets.............................................................................................................4
2. System Overview..........................................................................................................................5
2.1 Components of Olink Signature Q100 ......................................................................5
2.2 Olink Signature Q100 Interface Plates......................................................................6
2.3 Supported Chips.................................................................................................................6
2.4 Supported Panels...............................................................................................................7
2.5 Instrument Specifications...............................................................................................7
2.6 Air Options ............................................................................................................................7
2.7 Ethernet Option ..................................................................................................................8
2.8 Regulatory Compliance....................................................................................................9
3. Olink Signature Q100 Operation.........................................................................................11
3.1 Olink Signature Q100 System Software .................................................................11
3.2 User Accounts....................................................................................................................22
3.3 Best Practices....................................................................................................................26
4. Olink Signature Q100 Workflows.......................................................................................28
4.1 Workflow Overview ........................................................................................................28
4.2 Olink Target Workflows with the 96.96 and 48.48 chip..................................30
4.3 Olink Focus Workflow with the 24.192 chip .........................................................40
5. Troubleshooting.........................................................................................................................48
5.1 Run Instrument Checks.................................................................................................50
5.2 Export Instrument Check Logs................................................................................... 51
5.3 Replace the Fuse.............................................................................................................. 52
5.4 Shut Down the System ................................................................................................. 52
5.5 Start Up the System .......................................................................................................54
5.6 Shut Down and Turn Off the System ......................................................................54
5.7 Turn On and Start-Up the System ........................................................................... 55
5.8 Abort a Run in Progress................................................................................................ 56
5.9 Idle mode ............................................................................................................................56
6. Preventive Maintenance, Decontamination, and Disposal...................................... 57
6.1 Preventive Maintenance............................................................................................... 57
6.2 Decontamination and Disposal .................................................................................59
7. Technical support...................................................................................................................... 61
8. Revision history..........................................................................................................................62
9. Appendix: Related Documents............................................................................................63
10. Appendix: Safety .......................................................................................................................64
10.1 General Safety...................................................................................................................64
10.2 Instrument Safety............................................................................................................64
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1. About This User Manual
This user manual describes how to use the Olink® Signature Q100 system, including the instrument and the system
software. For complete instrument specifications and unpacking/installation instructions, see Olink Signature Q100 Site
Requirements.
IMPORTANT: Before using the Olink Signature Q100 system, read and understand the safety guidelines in this
document. Failure to follow these guidelines may result in undesirable effects, injury to personnel, and damage
to the instrument or property.
For complete safety information, see “10. Appendix: Safety”.
1.1 Safety Alert Conventions
Olink documentation uses specific safety alert conventions for presenting information that may require your attention.
Refer to Table 1 and Table 2 for safety alert conventions for chemicals and instruments respectively.
1.1.1 Safety Alerts for Chemicals
For hazards associated with chemicals, this document follows the United Nations Globally Harmonized System of
Classification and Labelling of Chemicals (GHS) and uses indicators that include a pictogram and a signal word that
indicates the severity level:
Table 1. Safety alerts for chemicals
Indicator Description
Pictogram (see example) consisting of a symbol on a white background within a red
diamond-shaped frame. Refer to the individual safety data sheet (SDS) for the applicable
pictograms and hazards of the chemicals used.
DANGER Signal word that indicates more severe hazards.
WARNING Signal word that indicates less severe hazards.
1.1.2 Safety Alerts for Instruments
For hazards associated with instruments, this document uses indicators that include a pictogram and signal words that
indicate the severity level:
Table 2. Safety alerts for instruments
Indicator Description
Pictogram (see example) consisting of a symbol on a white background within a black
triangle-shaped frame. Refer to the instrument user guide for the applicable pictograms
and hazards of the instrument usage.
DANGER Signal word that indicates an imminent hazard that will result in severe injury or death if
not avoided.
WARNING Signal word that indicates a potentially hazardous situation that could result in serious
injury or death if not avoided.
CAUTION Signal word that indicates a potentially hazardous situation that could result in minor or
moderate personal injury if not avoided.
IMPORTANT Signal word that indicates information necessary for proper use of products or successful
outcome of experiments.
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1.2 Safety Data Sheets
Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals ordered from Olink, either alone
or as part of this kit, go to olink.com and search for the SDS using either the product name or the part number.
Some chemicals referred to in this user guide may not have been provided with your system. Obtain the SDSs for
chemicals supplied by other manufacturers from those manufacturers.
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2. System Overview
The Olink Signature Q100 system includes optical, thermal cycling, and software components necessary to perform
protein expression analysis using proximity extension assay technology.
The Olink Signature Q100 system provides throughput for proteomics that is higher by orders of magnitude than
conventional platforms using Integrated Fluidic Circuits (Chip; from now on referred to as chips)—nanofluidic circuits
containing fluidic networks that automatically combine sets of samples with sets of primers. This innovative solution for
real-time PCR provides experiment densities far beyond what is possible with microplate platforms, significantly reducing
the number of liquid-handling steps and volumes per reaction.
2.1 Components of Olink Signature Q100
The Olink Signature Q100 system has a built-in computer that controls instrument operation and monitors its
performance. The onboard computer is operated only through the touchscreen.
Touchscreen
Drawer (retracted)
Power button
USB 3.0 port
Figure 1. Olink Signature Q100 front view
NOTE: The port on the front of the instrument supports USB 3.0. We recommend using a USB drive compatible
with USB 2.0 or higher.
Olink Signature Q100 side view
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POWERED BY
FLUIDIGM
LAN
AC 100–240V
50–60Hz, 8A
USBUSB HDMI
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
Compressed air input
(optional)
USB 2.0 ports
HDMI port
Ethernet port
Main power switch
Fuse
Power socket
Figure 2. Olink Signature Q100 rear view
2.2 Olink Signature Q100 Interface Plates
The Olink Signature Q100 interface plates are specific to the type of chip you are using. Store interface plates in the
storage container when not in use. The 96.96 Interface Plate comes with the Olink Signature Q100 system.
Component Label Color Supported chip
Olink Signature Q100 Interface Plate, 96.96
(96010)
Green Olink 96.96 chip for Protein Expression
Olink Signature Q100 Interface Plate, 48.48
(96011)
Blue Olink 48.48 chip for Protein Expression
Olink Signature Q100 Interface Plate, 24.192
(96012)
Red Olink 24.192 chip for Protein Expression
NOTE: Each interface plate can be purchased individually from Olink.
Figure 3. Olink Signature Q100 interface plates
2.3 Supported Chips
Olink Signature Q100 supports the following chips:
• Olink 96.96 chip for Protein Expression (95007)
• Olink 48.48 chip for Protein Expression (93007)
• Olink 24.192 chip for Protein Expression (99007)
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2.4 Supported Panels
Olink Signature Q100 supports the use of the following panels:
• Olink® Target 96 panels
• Olink® Target 48 panels
• Olink® Focus panels
2.5 Instrument Specifications
Refer to the Olink Signature Q100 Site Requirements Guide for instrument specifications and the recommended
environmental conditions.
2.6 Air Options
The Olink Signature Q100 system uses compressed air to pressurize fluids and control channels in the chip. The instrument
contains an internal compressor that draws in ambient air by default. Optionally connect it to an in-house air source.
NOTE: Use a clean, dry air (CDA) system whenever possible to help prevent corrosion within the pneumatic
system.
To use in-house compressed air instead of running the internal compressor, attach 4 mm tubing into the compressed air
inlet on the rear of the system and regulate the incoming air to 80–90 psi or 5.5–6.2 bar of CDA.
POWERED BY
FLUIDIGM
LAN
AC 100–240V
50–60Hz, 8A
USBUSB HDMI
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
Compressed air input
(optional)
Figure 4. Compressed air inlet
2.7 Ethernet Option
If you connect the Olink Signature Q100 instrument to your network, you can use domain authentication to manage user
accounts, import data directly from the instrument using the NPX Signature software, or enable remote technical support
through ethernet. For more information about domain authentication, “3.1.12 Enable Domain Authentication”.
NOTE: To enable NPX Signature remote data functions for the Q100 instrument, ensure that the instrument is
connected to the network and that TCP port 8085 (enabled by default) is not blocked.
NOTE: For information about importing run data in NPX Signature software, see the Olink NPX Signature User
Manual.
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POWERED BY
FLUIDIGM
LAN
AC 100–240V
50–60Hz, 8A
USBUSB HDMI
COMPRESSION AIR INLET
(OPTIONAL)
70–90 PSI ONLY
Ethernet port
Figure 5. Ethernet port
2.7.1 Network Requirements
When adding Signature Q100 to network, it is necessary to add it to a secured segmented network that cannot access
internet. Olink will not take any responsibilities for the risks associated with networking of the instrument.
To connect the Signature Instrument to the network the following requirements must be met:
• A DHCP enabled network with a dynamic IP-address allocation.
• The Instrument must be prevented from reaching the Internet. (See Network Segmentation)
• Communication attempts to the Instrument must allow TCP port: 8085
2.7.2 Network Segmentation
2.7.2.1 Introduction
To handle any security issues in the Local Network and making sure that the risk to the connected equipment is as small as
possible Network Segmentation is an important key factor. By segmenting the network, the following advantages can be
reached:
• Damage control and limitation in case of an incident via the smaller attack surface. A smaller subset of network devices
is within the same scope.
• Improved access control for external and internal network security.
–In this specific case the Signature Instrument should not reach the Internet and receive updates or be exposed to
threats.
• Reducing the attack plane and scope of compliance requirements related auditing
• Improved performance with less congestion on network traffic
• Better analytics around network monitoring, network access and network devices
• Endpoint device protection, especially important as IoT devices become more common
Network segmentation can boost your overall security policy by limiting access privileges to those who need it, protecting
the network from widespread cyberattacks and enabling better network performance by reducing the number of users in
specific zones.
2.7.2.2 How to implement Network Segmentation
This is heavily dependent on the flavor of Network Equipment used, and how to specifically implement segmentation can
only be answered when this is known. There are, however, a few basic technologies that is common for most solutions.
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• Firewall/router
–A firewall/router is used to handle multiple networks, and these can also be controlled through Access Rules. With
Access Rules we can prevent certain networks to reach resources they should not as well as protecting the network
resources from being reached from untrusted source. A capable firewall is also able to do much more, like web
filtering, intrusion prevention, antispam protection and preventing virus attacks.
o In a Network Segmentation Scenario, a VLAN or a separate LAN for the instrument should be created. A VLAN
(Virtual Local Area Network) has the advantage to use the same switches as other network segments – thus
reducing cost and space.
• Switch
–A Network Switch is another key component and utilizing VLANs can be used to segment the network. By setting
specific VLANs on different ports the switch can be segmented, and the different networks can be isolated.
Together with the Firewall, traffic between the networks can be established if the scenario requires it.
2.8 Regulatory Compliance
The following directives and harmonized standards were used to evaluate the safety and performance of the Olink
Signature Q100 system:
2.8.1 General Regulations and Requirements
• 2014/35/EU European Parliament Low Voltage Directive
• 2014/30/EU European Parliament Directive: Electromagnetic Compatibility
• 2011/65/EU Restrictions on the use of certain hazardous substances in Electrical and Electronic Equipment (RoHS)
• 2012/19/EU Waste Electrical & Electronic Equipment (WEEE)
• EU Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH), Regulation (EC) No 1907/2006
2.8.2 Harmonized Standards
• EN 61326-1
• IEC 61010-1 (including EN, CAN/CSA22.2, UL national deviations)
• IEC 61010-2-010
• IEC 61010-2-081 (including CAN/CSA22.2 national deviations)
2.8.3 Conformity Symbols on the Instrument
The instrument has the following conformity markings:
Conformity mark Description
Indicates conformity with safety requirements for Canada and the UnitedStates.
Indicates conformity with European Union requirements for safety and
electromagnetic compatibility.
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3. Olink Signature Q100
Operation
3.1 Olink Signature Q100 System Software
3.1.1 Unlock the Touchscreen
Swipe toward the unlock icon to unlock the touchscreen.
3.1.2 Log In to the Olink Signature Q100 System
If required, enter your username and password to log in to the system.
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After logging in, you have the following options:
• Home screen: Start a new run.
• History screen: View run logs for previous runs, export run data, and delete runs.
• Settings screen:
–System: View system information (GUI version, IP address), enable or disable user authentication at login, change
the length of time before the screen locks, change the scheduled time for the automatic periodic instrument checks
to run, change the time settings for the system.
–User Accounts: Create users, change user permissions, disable or delete user accounts, and reset user passwords.
To access the User Accounts settings, you must have an account that has Manager permissions (see section "3.2
User Accounts").
–Instrument Check: Run instrument checks on the Olink Signature Q100 instrument and export instrument logs.
For more information, see section “5.1 Run Instrument Checks”.
–Maintenance: The Maintenance settings can only be accessed by service personnel.
3.1.3 Start a New Run
To start a new run, tap the appropriate format for your chip type (Target 96, Target 48, or Focus). See “4. Olink Signature
Q100 Workflows” for instructions about specific workflows.
• For the Target 96 and Target 48 runs, see “4.2 Olink Target Workflows with the 96.96 and 48.48 chip”.
• For the Focus run, see “4.3 Olink Focus Workflow with the 24.192 chip”.
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3.1.4 Onscreen Help
Olink Signature Q100 includes an on-screen help system. During the process steps of a run, tap to open a new window
that contains instructions for how to perform the tasks required for that step of the workflow.
To close the on-screen help window, tap in the top right corner.
3.1.5 View Run Logs and Export Data
Tap History to
• view run details for previous runs
• export run data
• export comprehensive logs
• delete run logs. Runs that have been exported show a checkmark ( ) in the right column.
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3.1.5.1 Export Data from a Run
1. Insert a USB drive into the USB port on the front of the instrument.
2. Identify the desired run log in the History screen.
3. Select and export the run files:
–Tap or in the Export column to select a run, then tap ,
or
–Tap a run to view the run details screen, then tap Export.
The files are copied to the USB drive. A Success message is displayed when the export is complete.
If there was a problem with the run, Technical Support might request the raw data files for that run. Insert a USB drive
into the USB port on the instrument’s front, then tap Service Export to export the raw data. Send the files to Technical
Support.
NOTE: The service export files can be up to 2 or 3 GB. The percent completed is displayed on the screen while
the files are copied to the USB drive.
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3.1.5.2 Export Data from Multiple Runs
1. Insert a USB drive into the USB port on the front of the instrument.
2. In the History screen, tap or in the Export column to select each run you want to export.
3. Tap (Export).
The files are copied to the USB drive. A Success message is displayed when the export is complete.
3.1.6 Delete Run Files
After you export run files, you can delete them from the instrument to free up storage.
The hard drive of the system can hold approximately 350 runs. The system displays a message when you have 15%
capacity remaining.
1. In the run details screen, tap .
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2. Tap Yes, delete to delete the selected run folder. If you do not want to delete the run, tap No. A Success message is
displayed when the deletion is complete.
3.1.7 Delete Multiple Run Files
You can delete the already exported run files. In the History screen, exported runs show a checkmark ( ) in the right
column.
NOTE: Run files must be exported before they are deleted.
1. Go to the History screen.
2. Tap or in the Export column to select each run you want to delete, then tap .
3. Tap Yes, delete to delete the selected run folders. If you do not want to delete the runs, tap No. A Success message is
displayed when the deletion is complete.
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3.1.8 Change System Time Settings
1. Tap Settings > System, then tap (Edit) next to Time Settings.
2. Change the time zone and the date and time, then tap OK.
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3. To view the time in 24-hour format, select the 24 Hour Format checkbox.
4. Tap Save Changes. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
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3.1.9 Change the Periodic Instrument Check Scheduled Time
1. Tap Settings > System, then tap (Edit) next to Periodic Instrument Check.
2. Change the time of day you want the automated instrument checks to occur, then tap Save Changes.
NOTE: The time interval for the automated checks is 30 days and cannot be changed.
3. Tap Yes to update the system settings. Tap Cancel if you do not want to keep your changes.
For information about running instrument checks manually, see “5.1 Run Instrument Checks”.
3.1.10 Change the Screen Lock Timeout
1. Tap Settings > System, then tap (Edit) next to Screen Lock Timeout.
Choose the amount of time you want your screen to stay on after the last user touches it, then tap Save Changes. If you do
not want the screen to lock, clear the Enabled checkbox.
2. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
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NOTE: To lock the screen before the specified time has elapsed, swipe the button toward the lock icon in the
top right corner of the screen.
3.1.11 Enable or Disable User Authentication
You can require users to log in to the instrument with a password.
IMPORTANT: To enable or disable user authentication, you must log in with an account with Manager
permissions.
1. Tap Settings > System, then tap (Edit) next to Authentication.
2. Enter a Manager username and password (contact Olink Technical Support if you are the instruments Manager and
you don't have access to username and password.).
3. Tap to enable or disable the Require Authentication checkbox, then tap Save Changes.
4. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
After enabling authentication, you can access the User Accounts settings and add or remove users and change user
information. For more information about user accounts, see “3.2 User Accounts”.
3.1.12 Enable Domain Authentication
When user authentication is enabled, you have the option to use your lab’s domain authentication system to manage user
accounts.
To enable domain authentication for your instrument:
1. Create the following user groups in the lab’s Windows Active Directory:
–Olink Instrument Operator
–Olink Instrument Manager
2. Add users to the appropriate user groups.
3. Connect an Ethernet network cable to the port on the back of the instrument.
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4. Plug the instrument Ethernet cable into an active port that can communicate with the lab’s active directory.
5. Enable authentication on the instrument:
a. Tap Settings > System, then tap (Edit) next to Authentication.
b. Tap to enable the Require Authentication checkbox, tap Save Changes, then tap Yes to update the system
settings.
After enabling authentication you can log in to the instrument using your Windows Domain credentials.
3.2 User Accounts
After enabling authentication, you can access the User Accounts settings and add or remove users and change user
information.
NOTE: To obtain or reset the Manager password, contact Technical Support to request a recovery key.
The Olink Signature Q100 system has three types of user accounts:
Account Type Description
Manager Managers can:
• Log into the system
• Start new runs and abort runs started by any user
• Add new users
• Change other users’ settings, including permissions and password
Operator Operators can:
• Log into the system
• Start new runs and abort runs that they started
Operators cannot:
• Operators cannot abort a run started by a different user.
• Delete user accounts
Disabled A user with a Disabled account is no longer allowed to log in, but the account is still in
the system for auditing purposes.
3.2.1 Add a New User
IMPORTANT: To add a new user, enable user authentication, and log in with an account with Manager
permissions.
1. Log in to the Olink Signature Q100 system as a Manager.
2. Tap Settings > User Accounts.
3. Tap (New User).
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